Prescription teaching is a routine task in medical colleges.However it is not a separate course,which can be undertaken by different teaching units or courses,and is not mentioned in training program.So,there are many problems in teaching practice,such as indefiniteness in responsibility,simplification of teaching settings,maladjustment to teaching and training objective and so on.Thus administrative department and major leaders should deepen the understanding of prescription teaching,take full account of its position in planning the training program,and make it more scientific to meet the needs of professional training.

Immunocytochemistry, flowcytometry and nucleic acid in situ hybridization were used to observe the effect of sodium tanshinon Ⅱ-A silate (DS201) on proliferation of pulmonary artery smooth muscle cell (PASMC) induced by hypoxic endothelial cell condition medium (HECCM). The results showed that DS201 could obviously hinder PASMC to enter S phase from G0/G1 phase (P<0.05), and decrease the expression of PCNA and platelet-derived growth factor (PDGF)-A and B chain mRNA in PASMC respectively, (P<0.01). It was suggested that DS201 could probably inhibit the prolifertion of PASMC mediated by HECCM, through down-regulating the expression of PDGF-A and B chain gene.

Polycystic ovary syndrome (PCOS) is a common endocrine metabolic disorder of reproductive age women,often accompanied by obesity,hyperandrogenism,hyperinsulinemia,insulin resistance (IR),and other metabolic syndrome (MS).Oxidative stress (OS) is considered as a potential inducing factor in the pathogenesis of PCOS at present.Glucagon-like peptide-1 (glp-1) receptor agonist and analogues as new therapeutic agents in the treatment of type 2 diabetes mellitus (T2DM),can lose weight,improve IR,resist OS and reduce the expression of inflammatory cytokines,thus they have been widely used in the clinical application of patients with PCOS.The author reviews the latest advances of glucagon-like peptide-1 on polycystic ovary syndrome.

Objective To study the application of multi‐tumor marker protein chip for early tumor screening and diagnosis . Methods From Nov .2011 to Dec .2015 ,10 736 samples ,including people receiving physical examination and with high risk of canc‐er in Fengcheng Hospital were collected .Twelve tumor markers in serum(AFP ,CEA ,NSE ,CA125 ,CA153 ,CA242 ,CA199 ,PSA ,f‐PSA ,FER ,β‐HCG and HGH)were measured by multi‐tumor markers protein chip detective system ,and the results were analyzed . Results We found out 967 samples with positive markers in the 10 736 samples .Of which ,496 were male and 471 were female ,the positive rate were 4 .62% and 4 .39% respectively .Totally 473 were diagnosed with tumor confirming by clinical pathology ,postive diagnosis rate was 48 .91% .Conclusion The multi‐tumor marker protein chip (C12 system) can detect multiple tumor markers simultaneously to improve screening process and achieve rapid detection ,w hich has higher positive detectiong rate and clinical value on diagnosing malignant tumor in early stage .

Objective To investigate the clinical value of serum CEA, CA19-9 and CA724 in patients with colonic carcinoma. Methods Serum levels of CEA, CA19-9 and CA724 were detected with ELISA in 89 patients suffering from colonic cancer. Results The positive rates of CEA, CA19-9 and CA724 in Dukes' stages C and D patients were remarkably higher than those in Dukes' stages A and B patients(64% vs 26%,46% vs 22%,26% vs 8% respectively, P

Objective　To study the predictive value of islet cell antibody (ICA),64KD protein antibody(64KDAb) and insulin auto-antibody (IAA) in the first-degree relatives of type 1 diabetics.Methods　The determination of islet autoantibody distribution in type 1 diabetes patients,first-degree relatives without diabetes and normal individuals was made.Results　The results revealed that ICA and 64KDAb positive rate in type I diabetes patients and their first-degree relatives was significantly higher than normal individuals.The positive rate of 64KDAb is higher than that of ICA and IAA. Conclusion　These results suggest that 64KDAb's sensitivity is higher than ICA and IAA's,so 64KDAb is more valuable as the marker of predicting type 1 diabetes.The combined determination of ICA,64KDAb and IAA might improve the value for predicting diabetes.

Objective:To investigate the mechanism of association between Pregnancy induced Hypertension and HLA G.Methods:The distribution and frequencies of HLA G alleles were analyzed in 54 PIH patients and 146 random healthy controls with nonradioactive digoxigenin labeled PCR SSO method.Results:The results showed that the same nine HLA G alleles were detected and the G*01011 was the commonest allele with the frequency of 31.38% and 37.33% respectively in both PIH and controls groups,the next was G*01013,25.00% and 20.21% separately.Statistic analysis showed that the frequencies of HLA G alleles were similar to in the two groups and there was no significant difference between PIH patients and controls.Conclusion:These data suggest that there is no significant detectable relation between the HLA G polymorphism and PIH.

Objective To investigate the therapeutic efficacy of surgical treatment of myocardial bridge. Methods Fifteen patients with myocardial bridge had been treated in our hospital from January 1999 to October 2006. Thirteen patients who had typical angina had been treated regularly by medication, but their symptom remission was not satisfactory. One patient suffered from acute myocardial infarction, and another 1 patient complained of palpitation and syncope. All the patients were given surgical treatment. Surgical strategies included minimally invasive off-pump surgery in 9 patients (supraarterial myotomy in 3 patients, coronary artery bypass grafting in 2, and coronary artery bypass grafting with supraarterial myotomy in 4) and on-pump surgery in 6 patients (coronary artery bypass grafting with supraarterial myotomy in 5 patients and supraarterial myotomy in 1). Results All the operations were successfully completed. The operative duration of off-pump and on-pump surgery was 2.8?1.9 h and 3.5?1.7 h, respectively. The extracorporeal circulation time was 59?37 min. No surgery related death or complications occurred. At 3 months after operation, all the patients were free from symptoms, and electrocardiogram returned to normal in 11 patients. During a follow-up for 0.5~7 years (1.9?1.2 years), recurrent palpitation was seen in 1 patient and others reported no angina. Conclusions Surgical treatment of myocardial bridge has good immediate and long-term results.

Objective To study the predictive value of islet cell antibody (ICA),64KD protein antibody(64KDAb) and insulin auto-antibody (IAA) in the first-degree relatives of type 1 diabetics. Methods The determination of islet autoantibody distribution in type 1 diabetes patients,first-degree relatives without diabetes and normal individuals was made. Results The results revealed that ICA and 64KDAb positive rate in type I diabetes patients and their first-degree relatives was significantly higher than normal individuals. The positive rate of 64KDAb is higher than that of ICA and IAA. Conclusion These results suggest that 64KDAb's sensitivity is higher than ICA and IAA's,so 64KDAb is more valuable as the marker of predicting type 1 diabetes. The combined determination of ICA, 64KDAb and IAA might improve the value for predicting diabetes.

Objective To study the normal value on the motor conduction velocity(MCV) and F wave parameters in normal adults at different age,sex,and provide reasonable datum for clinic.Methods 155 healthy volunteers (male for 76 cases, female for 79 cases,age from 18 to 82 years old) were used as subjects.Distal latency,proximal latency, MCV,F wave latency,the duration between F and M waves (F MIPL),F wave conduction velocity (FCV) as well as the F wave ratio were recorded in bilateral median and ulnar nerves, F wave were elicited with stimulation at wrist.Results There were significant differences in M wave latency, MCV, FwPL, F MIPL and FCV between the different groups of age and sex, the latency of each individual wave was shorter in the female than in the male.the conduction velocity(CV) of the female was faster than the male. No significant differences in the F wave ratio were found between male and female, left and right limbs for the same nerve,median and ulnar nerves in the same Limb and in individuals at different age.MCV was usually faster in the proximal than in the distal.Conclusion MCV and F wave parameters changed progressively with increasing age. There were respective normal values in different age group and sex,F wave ratio might provide information in evaluating nerve conduction function in both of the proximal and distal end.