Objective To conduct the performance verification of the Stago STA-R automatic blood coagulation analysis system once every year to evaluate the analysis system for ensuring its accuracy and reliability .Methods According to the related docu-ments of the Clinical and Laboratory Standards Institute (CLSI) ,5 items of prothrombin time (PT ) ,activated partial clotting en-zyme live time (APTT) ,thrombin time (TT) ,fibrinogen (Fib) and antithrombin Ⅲ (ATⅢ) were selected to conduct the verifica-tion and preliminary evaluation on the accuracy ,imprecision ,carry-over rate ,linearity range and reference interval of the instrument analysis system .Results The relative bias(SE% ) of accuracy in each item was less than 5% .The intra-batch and inter-batch im-precisions (CV) were less than 3% and 5% respectively .The carry-over rates were within 4% .The linearity of Fib and AT Ⅲ was very well ,r2 were 0 .9980 and 0 .9979 respectively .Validated reference interval was consistent with quotative reference range . Conclusion The Stago STA-R automatic blood coagulation analysis system has good analytical performance ,can provide accurate and reliable test results for clinical doctors .

The humanistic thinking of life philosophy of traditional Chinese medicine (TCM) observed inlife activities is different from western theories and methods,which is more about life experience and feeling and rich in practical significance.The life philosophy of TCM is also different from traditional Chinese culture.The intuitive feeling of life is one of the main characteristics of life philosophy of TCM.Students of TCM soak in traditional Chinese medicine culture and are influenced by the thinking of real life caused by the care of realbody in TCM.Chinese medicine education should fully mobilize the tacit knowledge of life education in TCM,play excellent tradition of Chinese traditional life philosophy,and cultivate the life moral quality in students of TCM.

[Objective] To discuss the therapeutic effect of different methods in the treatment of intertrochanteric femur fracture. [Methods]Totally 118 middle aged or old patients with intertrochanteric femur fracture were fixed with dynamic hip screw (DHS), Gamma nail or PFNA.[Results]Totally 112 cases were followed up, and the average time was 16 months, in those 45 patients were fixed with DHS: 29 were excellent, 11 cases were good, and 5 were poor, and the excellent to good rate was 88.9%; 36 patients were treated with Gamma nail, and the excellent, good, poor results were 28, 2, 1, respectively, and the excellent to good rate was 96.8%; 36 patients were fixed with PFNA, and the excellent, good, poor results were 29, 6, 1, respectively. The differences were significant (P=0.02, analyzed by Logistic regression with SPSS software). The excellent and good superiority of Gamma nail and PFNA were 5.1552 and 2.3726 times compared to DHS, and there was significant differences between the groups.[Conclusion]Surgical treatment of intertrochanteric femur fracture can improve the functional recovery and reduce the complication incidence, and PFNA fixing method can shorten the operative time, reduce the bleeding and the therapeutic effect is reliable, and suitable for old patients combined with osteoporosis.

Objective It has been shown that ketamine and magnesium used in combination provide more effective analgesia than either drug alone. The purpose of this study was to investigate the effects and interaction of the two drugs on recombinantly expressed NMDA receptors. Methods Xenopus oocytes expressing NR1/NR2A or NR1/NR2B glutamate receptors were stimulated with glutamate/glycine and studied at a holding potential of -70mV using two electrode voltage clamp. The effects of magnesium and s ( + )-ketamine alone or in combination on NMDA signaling were determined. Results Magnesium and s( + )-ketamine alone inhibited NMDA receptors non-competitively. IC50s of magnesium were (4.2 ± 1.2)×10-4mol/L and (6.3 ± 2.4) × 10-4 mol/L on NR1/NR2A and NR1/NR2B, while IC50s of s( + )-ketamine on NR1/NR2A and NR1/NR2B were (4.1 ± 2.5) × 10-6 mol/L and (3.0 ± 0.3 ) × 10-6 mol/Lrespectively. Magnesium and s( + )-ketamine used in combination decreased IC50s more than 90% at both receptors. Isobolographic analysis demonstrated superadditive interactions. Conclusions s( + )-ketarnine and magnesium inhibit responses of recombinantly expressed NMDA recptors non-competitively, and the combinations of the two drugs produces a synergistic effect.

Objective To evaluate the efficacy of telephone follow-up on glycemic control in patients with diabetes mellitus. Methods Randomized clinical trials that assessed the effectiveness of telephone follow-up in patients with diabetes mellitus were selected in the databases of Cochrane library, JBI, PubMed, Medline, EMBASE, EBSCO, OVID, Springer, China National Knowledge Infrastructure, China Biomedical Literature Database, VIP , Wangfang Data. At the same time manual retrieval of relevant professional information to collect randomized clinical trials that met the inclusion criteria. The searching time was from database establishment to June 2016. RevMan5.2 software was used to analyze the data after quality assessment. Results A total of 12 randomized controlled trials were included in a total of 1905 patients. Meta-analyses showed that, compared with the conventional nursing group, the telephone follow-up could significantly reduce the level of glycosylated hemoglobin (weighted mean difference was-0.49, 95% confidence interval was-0.52--0.46, P<0.01), the level of fasting blood glucose (weighted mean difference was-0.99, 95% confidence interval was-1.16--0.83, P<0.01). Conclusions Telephone follow-up can improve blood glucose control in patients with diabetes, it is worth to be popularized in clinical practice.

Objective To evaluate feasibility, effectiveness , safety, and complications of superselective bronchial artery embolization with coaxial microcatheter system for hemoptysis. Methods 32 times of superselective bronchial artery embolization were performed for 29 cases with hemoptysis resulted from a variety of causes, age grade from 35 to 71(mean 54) year old. The causes of hemoptysis included bronchiectasis (9 cases), tuberculosis (12 cases), and pulmonary carcinoma (8 cases). All studies were performed with standard percutaneous transfemoral catheterization. Results The success rate of superselective catheterization was 96 9% (32/33). Hemoptysis control was achieved in 96 9% (31/32), with complete control rate of 65.6% (21/32) and partial control rate of 31.3% (10 /32),inefficacy case:only one.One patient with bronchiectasis, 6 patients with pulmonary carcinoma and 3 patients with chronic fibrosis tuberculosis were included in the partial control group. Rebleeding occurred in 7 patients (4 patients with pulmonary carcinoma and 3 patients with chronic fibrosis tuberculosis) during 2 months to 32 months followed-up period. No severe complications occurred in the procedure. Conclusions Superselective bronchial artery embolization for hemoptysis with coaxial microcatheter is a safe, effective way to control hemoptysis, has excellent long term efficacy, and without severe complications.

Type 2 diabetes mellitus is a metabolic disorder characterized by hyperglycemia caused by multifactors.The incidence of type 2 diabetes is gradually increased.Its prevention and treatment are paid more and more attention.Intestinal flora is one of the hot spots in recent years.A large number of studies have shown that intestinal flora is closely related to the occurrence and treatment of type 2 diabetes mellitus.Intestinal flora is expected to be a new direction for the improvement and treatment of type 2 diabetes mellitus.In this paper,the relationship between type 2 diabetes and intestinal microflora in recent years,pathogenesis and treatment were summarized.

Plateau environment has a wide range of diurnal temperatures and thin air. The surgical shelter needs to resist the huge temperature shock and work in harsh environments, placing higher noise immunity requirements on the microenvironment temperature control system. The traditional microenvironment control of surgical treatment shelters mostly use instrument display and switch control, with a high failure rate and functional dispersion. We have designed a plateau microenvironment temperature control system with PLC as the control center and the touch screen as the Human Machine Interface so that real-time monitoring and control of temperature is achieved and the plateau temperature shock is resisted while the heater fuel ratio can be adjusted properly to adapt to plateau environment. This paper describes the control system hardware, software and algorithm design in detail.

Objective To observe the effects of chronic levodopa administration on cerebral dopamine transporter in healthy aged SD rats.Methods Twelve healthy aged SD rats was divided into three groups,which was respectively gavaged with high-dose of levodopa (150 mg/kg), low-dose of levodopa (50mg/kg) and NS everyday for four months. After levodopa was stopped for twenty-four hours, 0.2 ml of 99mTc-TRODAT-1 (800 ?Gi) was injected into the rats through vena caudalis. Three hours later, the rats were sacrificed and the wet weight of striatum, cerebral cortex, cerebella and brain stem was weighed. We measured the counts of radiation and calculated the numerical value of ID.Results The activity of ID in striatum was ( 0.764? 0.129) in high-dose group and ( 0.928? 0.145) in low-dose group. Compared with the control group ( 1.562? 0.289), the differences were significant ( P

Objective To introduce a new CT classification system we designed for Hoffa fractures and compare the interrater reliability between the CT and X-ray classification systems.Methods A total of 20 isolated Hoffa fractures from January 2008 to December 2011 were randomly selected for the present analysis of their imaging data (anteroposterior and lateral X-ray films of the knee joint and three-dimensional CT reconstruction of the femoral condyle).At the same time,a total of 20 independent observers (clinicians with junior,intermediate and senior professional qualifications) were selected for classification of the Hoffa fractures in the same manner respectively according to the Letenneur's X-ray system and our self-designed CT system.We used Kappa statistics to evaluate the interrater reliability among the clinicians between the 2 classification systems for Hoffa fractures.Results According to the CT classification of the 20 Hoffa fractures by the 20 clinicians,type Ⅰ,Ⅱ and Ⅲ fractures accounted for 66.0％,30.5％ and 3.5％ respectively.In type Ⅰ fractures,type Ⅰb involving zone b accounted for the most (50.0％),next by type Ⅰc(31.0％) and type Ⅰa (19.0％).In type Ⅱ comminuted fractures,fracture fragments were mostly seen in zone b.The overall incidence of fractures involving zone b by CT classification was 67％.According to the X-ray classification,type Ⅰ,Ⅱ,Ⅲ and Ⅳ fractures accounted for 31.4％,14.3％,28.0％ and 26.3％,respectively.The interrater reliability for CT classification agreement (Kappa =0.681) among clinicians was higher than that for X-ray agreement (Kappa =0.261).Conclusion For Hoffa fractures,communicated ones in particular,our CT classification system may be better than the X-ray classification system.