Objective: ①To detect the concentrations of interleukin 1?(IL 1?) and prostaglandin E 2 (PGE 2) in periapical exudates in the patients with acute or chronic periapical periodontitis; ② To examine their correlations with clinical and radiographic findings of the involved teeth.Methods: The volume of periapical exudates was quantified using the standard paper point sampling method; the concentration of IL 1? and PGE 2 was detected using enzyme linked immunosorbent assay(ELISA) or radioimmunoassay(RIA) in 40 cases with acute and in 40 with chronic periapical periodentitis. Results: ①The volume(?l) of the exudates in the cases with acute periapical periodontitis and chronic were 27.10?15.70 and 3.12?2.88( P

Objective To observe the diameters of macular hole and destroyed boundary line between inner and outer segment (IS/OS) of the photoreceptors and the correlation with the visual acuity in patients with idiopathic macular hole(IMH). Methods The clinical data of 39 eyes (37 patients) with IMH were retrospective analyzed. All the patients had undergone the examinations of visual acuity (Snellen chart), intra-acular pressure, ocular fundus (indirect ophthalmoscope), and Fourier-domain optical coherence tomography (FD-OCT) whose speed was 27 000 A scan/s, area was 6.0 mm×6.0 mm, and the mode was 512×128. The diametres of macular hole and destroyed IS/OS, and the correlations with visual acuity were detected. Results The mean IogMAR was 0.99±0. 44 (ranged from 0. 15 to 2.00), the mean diameter of macular holes was (942.0± 348. 4)μm(ranged from 171 to 1491 μm), and the mean diameter of IS/OS disruption was (1870. 35±673.2) μm(range from 463 to 3176 μm). There was a significant correlation among the diameter of the macular hole, the diameter of the IS/OS disruption, and IogMAR in IMH (P= 0. 038, 0. 002, 0. 000). In eyes with closed macular hole after surgery, the IogMAR and the diameter of the IS/OS disruption had a significant decrease. Conclusion Using FD-OCT the photoreceptor changes can be visualized in vivo. The abnormality in the IS-OS boundary line appears to be involved for a much larger area beyond the macular hole itself, and persists there with small size even after the maeular hole closure surgery.

Objective To investigate the cognition, self-management and quality of life of osteoporosis in elderly patients, and to provide the basic theory for health education in elderly patients with osteoporosis.Methods 565 elderly patients with osteoporosis in our hospital from May 2012 to Mar.2015 were enrolled.The cognition, health beliefs, self-efficacy and quality of life were investigated by questionnaires and analyzed by Microsoft Office Excel 2007.Results 565 osteoporosis patients, the symptom score was (15.6±2.0), and the score was lower in sporting knowledge (4.3 ± 1.8) than in calcium knowledge (5.7 ± 1.5) (t=2.784, P=0.011).Health beliefs score was (139.0±9.6), containing exercise benefits score of (21.5±3.1), exercise barriers score of (13.8±3.9), susceptible score of (19.4±4.2), serious score of (18.8±4.4), health motivation score of (25.0±3.0), calcium intake benefits score of (20.7±3.5) and calcium intake disorder score of (19.8 ± 4.0).Self efficacy score was (63.7 ± 19.4), containing exercise efficacy score of (58.6 ±18.7) and calcium efficacy score of (69.2± 18.5).Quality of life score included the general health (16.5± 2.9 score), social function (5.8 ± 1.3 score), vitality (14.8 ± 3.2 score), mental health (20.7± 4.2 score), physiological function (20.5 ± 4.1 score), physiological role limit (6.3 ± 1.6 score), emotional role limit (5.1 ± 1.3 score) and body pains (4.3 ± 1.8 score).Conclusions The score in cognitive function is low in elderly osteoporosis patients.It's useful to improve elderly osteoporosis awareness and quality of life by carrying out relative education.

0. 05). No significant difference was found in maternal and neonatal complications between the ANCA( + ) and ANCA(－) subgroups in S-PE subjects. But those 4 cases who developed renal function insufficiency patients were ANCA( + ). Conclusions ANCA might be associated with renal diseases in preeclampsia women, and further studies is required to determine whether ANCA is involved with the pathogenesis of preeclampsia.

In recent years, the prevalence of cardiovascular disease (CVD) in China has been increasing year by year, and it has become a major public health concern. The prevention and control of chronic CVD is inseparable from effective early screening. However, due to the imbalance in the level of economic and social development in various parts of China, the universality and accessibility of screening in some areas still need to be improved. The fundus retinal blood vessel is the only vessel in the whole body that can be directly observed. Studies have found that its morphological and functional changes are related to the occurrence and development of many CVD, and it has the potential to become a tool for assessment and prediction. This review intends to introduce the research progress of retinal blood vessels in the evaluation and prediction of cardiovascular disease in recent years.

OBJECTIVETo explore the expression of hepatitis B-virus X gene (HBX) in adenoid cystic carcinoma (ACC) and determine its clinical significance.

METHODSBetween June 2008 and October 2012, in-hospital patients with salivary gland tumors who were treated at the Department of Oral and Maxillofacial Surgery, Affiliated Stomatology Hospital of Kunming Medical University, were enrolled to this study. HBeAb-positive patients were defined as those exposed to hepatitis B virus (HBV) or harboring persistent HBV infection regardless of being HBeAg positive or negative. According to the pathological results, all patients were divided into ACC group and control group. Immunohistochemical staining and polymerase chain reaction (PCR) were used to detect HBX expression in ACC group and control group.

RESULTSHBX expression was mostly detected in the cytoplasm of ACC cells. Minimal HBX expression was detected in the nucleus. HBX expression was significantly higher in ACC than in Warthin's tumor. A significant difference was observed between the two groups.

CONCLUSIONHBX is expressed in ACC and may be associated with the development of ACC. HBX might serve important functions in the carcinogenesis and development of ACC.

OBJECTIVE:To provide reference for improving teaching effects of Medicinal Chemistry Experiment,and cultivat-ing practical talents that in line with needs of the pharmaceutical industry. METHODS:Based on the training target of medicinal preparation major in our college,the basic idea of establishing Medicinal Chemistry Experiment system was discussed,and teach-ing target,content,process and experimental evaluation results of Medicinal Chemistry Experiment were analyzed in detail. RE-SULTS:The experiment teaching system was established,with teaching target of improving knowledge,ability and quality,teach-ing contents of basic experiment,comprehensive experimental design experiment and open experiment,center of developing practi-cal teaching from before class,in class and after class,and means of diversified performance evaluation. CONCLUSIONS:The es-tablished experiment teaching system is suitable for the teaching of Medicinal Chemistry Experiment in medicinal preparation ma-jor,it has created a new situation that professional,innovation and quality education organic integrate in teaching activity,mobi-lized the students’learning enthusiasm and initiative,exercised the students' professional knowledge and practical ability,cultivat-ed students' creative ability and comprehensive quality and enhanced the effect of experiment teaching.

BACKGROUND:Closed reduction and interlocking intramedullary nailing can protect blood supply of fractured bone,decrease infection and promote bone healing.which is becoming the first choice for treating femoral shaft fracture However,it is difficult to conduct in closed reduction and keep stabilization of fractured bone.OBJECTIVE:To explore the curative effect of closed reduction and interlocking intramedullary nailing in treating femoral shaft fracture with assist of rubber tourniquet 360°elastic fixation METHODS:From May 2008 to November 2009,18 patients(14 males,4 females;aged 18-65 years)with closed femoral shaft fracture were treated at the Dongguan Hospital of Traditional Chinese Medicine.Body skin was protected by cloth,elastic rubber tourniquet was doubled and 360°wrapped around the fractured bone with the length of 10 cm from up and down.Supracondylar femur was reamed by Kernig-needle;fractured bone was dealt with countertraction and horizontal rotation.Another assistant compressed the fractured bone according to the displacement of fracture,which contributes to bone fracture reduetion and interlocking intramedullary nailing.RESULTS AND CONCLUSION:All 18 patients were followed up postoperatively,no case was found with skim necrosis and damage of nerve and blood vessel.16 cases were bone union with excellent 15 cases,fine 1 case;2 cases were still being followed up.Callus was firstly found 1 month after operation and was obviously found in 3e months postoperatively The result suggested that the treatment of closed reduction and interlocking intramedullary nailing with assist of rubber tourniquet360°elastic fixation is a simple and applicable approach.which reduces the interruption of blood supply for fractured bone and closed reduction gets good result,which reduces the chance of open reduction.

Objective To summarize the clinical outcome of direct trans-scleral drainage by puncture with 25G needles in the external-route surgeries for primary retinal detachment. Methods Fifty-four consecutive cases of primary retinal detachment were treated by external-route surgeries. The subretinal fluid of 23 cases was drained by direct scleral puncture with 25G needles (25GND group), the other 31 cases underwent the conventional scleral incision and drainage(CD group). Postoperative complications, the single-operation retinal reattachment rate and functional outcome were recorded.Results Subretinal fluid was adequately drained in all cases. there was no significant difference in the single-operation retinal reattachment rate between 25GND and CD groups. In comparision to CD, 25GND significantly decreased postoperative complication rate (?2=4.729,P

Ferulof en ( FL, 45mg and 200mg/kg im ) showed marked antipyretic effect on rabbit fever induced by typho-paratyhoid vaccine and on rat fever induced by yeast powder suspension respectively. Their potency and action phase were similar to those of aspirin ( 35mg and 100mg/kg) . Furthermore, it blocked the action potential of sciatic nerve in toad to some extent and had a certain local anesthetic effect on guinea pigs in intradermic wheal test, but much weaker than procaine and lidocaine at the same concentration. It had no obvious influence on corneal reflex in rabbits.It can be concluded from these findings that together with the results of previous paper the findings of this paper further indicated FL may be proved to be a new antiinflammatory, antipyretic and analgesic drug.