1.Plasma markers of endothelial function in cardio-cerebrovascular diseases
International Journal of Cerebrovascular Diseases 2009;17(3):200-204
Endothelial cells can be activated, and develop dysfunction and damage under the pathological state. Endothelial dysfunction plays a key role in the processes of cardio-cerebrovascular diseases, and can be detected in peripheral blood. The concentration of von Willebrand factor (vWF) predicts the risk of coronary heart disease and stroke; the concentration of soluble thrombomodulin is associated with the prognoses of coronary heart disease and stroke; soluble intercellular ashesion rnolecule-1 (ICAM-1) predicts the risks of carotid athero-sclerosis, coronary heart disease, peripheral arterial disease, and diabetes mellitus; the levels of plasma soluble E-selectin in patients with carotid atherosclerosis, coronary artery disease, and diabetes mellitus are increased; the increased endothelin-1 levels predicts the elevated risk of congestive heart failure. The injection of endothelial progenitor cells is promising to become a novel approach for the treatment of cardio-cerebrovascular diseases.
2.Correlation between Carroll Upper Extremities Functional Test and Fine Motor Domain of Gesell Developmental Scale in Children with Cerebral Palsy
Chinese Journal of Rehabilitation Theory and Practice 2017;23(2):203-206
Objective To investigate if Carroll Upper Extremities Functional Test (UEFT) is a better rating scale for evaluating fine mo-tor function in children with cerebral palsy. Methods From January, 2011 to January, 2014, 104 children with cerebral palsy were divided in-to non-hemiplegic palsy group (n=76) and hemiplegic palsy group (n=28), they were assessed by two certified valuers in one week. Their fine motor function was evaluated with UEFT and the Gesell Developmental Scale. The scores of UEFT were compared respectively with the developmental age (DA) and developmental quotient (DQ) in fine motor domain of Gesell Developmental Scale. At the same time, the UEFT score of the affected limb was compared with of the unaffected limb in children with hemiplegic palsy. Results In the non-hemiplegic palsy group, the UEFT score strongly correlated with DA (r=0.752, P<0.001) and moderately correlated with DQ (r=0.456, P<0.001). In the hemiplegic palsy group, the UEFT score of the unaffected limb strongly correlated with DA (r=0.722, P<0.001), and a moderate correlation was found in the affected limb (r=0.591, P<0.001). The UEFT scores of both sides did not correlated with DQ (r<0.186, P>0.05). The UEFT score of unaffected limb was significantly higher than that of the affected limb (Z=-4.708, P<0.001). Conclusion Both of UEFT and the Ge-sell Developmental Scale could be used clinically in assessment of fine motor function in children with cerebral palsy. The UEFT was better in evaluating children with hemiplegic palsy.
3.Effects of glucose on mesenchymal stem cell proliferation in mice
Chinese Journal of Diabetes 2008;16(10):625-628
Objective To investigate effects of glucose on mesenchymal stem cells(MSCs) proliferation and the possible mechanism. Methods We evaluated the purity of MSCs,established different groups of glucose, detected proliferation by MTT, and analyzed cell cycle and apoptosis by a flow cytometry system. Results The purity of MSCs exceeded 90%.The positive rates of CD44, CD90, CD45, CD34 were 91.92%, 93.38%, 2.23%, 1.05% respectively.Glucose significantly affected MSCs proliferation(P=0.003):11 mmol/L glucose could promote MSCs proliferation, but 25 mmol/L glucose made this effect disappear. High glucose significantly increased the percent of S phase in cell cycle(P=0.002)and the rate of apoptosis(P=0.000). Conclusions Glucose has two-way regulation towards MSCs proliferation, and the balance between cell-cycle arrest and apoptosis could be responsible for this effects
4.Effect of targeted information support on the sense of uncertainty and anxiety among patients treated with percutaneous coronary intervention
Chinese Journal of Practical Nursing 2008;24(7):19-22
Objective To discuss the effect of targeted information support on the sense of uncertainty and anxiety among patients treated with percutaneous coronary intervention and its instructive significance to nursing work.Methods We randomized 118 patients treated with percutaneous coronary intervention whose sense of uncertainty and anxiety were relative intensive into the test group and the control group with 59 cases in each group.We gave routine health instructions to the control group while targeted information support besides routine health instructions to the test group.We investigated the patients in two groups by Chinese version of uncertainty scale and State Anxiety Inventory at 4 time points,that is ,the first day in hospital,night before the operation,24 hours after the operation and 1 day before leaving hospital.Results The anxiety level and sense of uncertainty about illness decreased at the night before operation,24 hours after operation and 1 day after operation compared with the control group.The sense of uncertainty and anxiety level has statistical difference between whatever two adjacent time points except for anxiety level between the time points between the first day in hospital and before operation.Drop of sense of uncertainty at 24 hours after operation was significantly different between the two groups(P<0.01).Conclusion Supplying targeted information support could decrease the sense of uncertainty and anxiety among patients treated with percutaneous coronary intervention.
5.Effect of Jianzhong Yuyang Tablets on Prostaglandin E_2 Content in Gastric Mucosa of Rats with Gastrointestinal Injury Induced by Indomethacin
Weihong KUANG ; Tongguang YANG
Journal of Guangzhou University of Traditional Chinese Medicine 2004;0(06):-
【Objective】To observe the effect of Jianzhong Yuyang Tablets(JYT) on prostaglandin E2(PGE2) content in gastric mucosa of rats with gastrointestinal injury induced by indomethacin.【Methods】One hundred and seven SD rats were randomized into 7 groups: blank control group,model group,famotidine(7.14 mg?kg-1?d-1)group,two Radix Codonopsis(RC,0.71 and 2.84 g?kg-1?d-1 respectively) groups,two JYT(0.27 and 1.08 g?kg-1?d-1 respectively) groups.Except the blank control group,the rats in other groups were given subcutaneous injection of indomethacin 20 mg/kg to induce gastrointestinal injury.PGE2 content in gastric mucosa of rats were detected with radioimmunoassay 7 hours and 7 days after modeling respectively.【Results】PGE2 content in gastric mucosa of model rats was lower than that in the blank control group 7 hours and 7 days after modeling(P
6.Evaluation of the sterilizing efficiency on absorbable poly-L-lactide screws with two sterilants
Yuexiong YANG ; Weihong YANG ; Jing LI
International Journal of Biomedical Engineering 2009;32(4):201-205
Objective To evaluate the sterilizing efficiency ofabscrbable poly-L-lactide (PLLA) screws sterilized with ethylene oxide and 60Co-γ radial. Methods One group of PLLA screws was sterilized in the ethylene oxide sterilization ease. In order to eliminate the rudimental ethylene oxide, the sterilized screws was put in the air for five days. The other group of PLLA screws was sterilized with 60Co-γ radial with absorbable dose of 15 kGy. Then all sterilized screws were characterized. Results Compared with the primal bend intensity of the PLLA screws, the bend intensity of sterilized screws did not descend remarkably (P>0.05). Compared with the primal molecular weight of the PLLA screws, the molecular weight of screws with 60Co-γ radiation sterilization re-markably decreased 11%. It indicated that the material had degraded. The molecular weight of screws with ethylene oxide sterilization ordy decreased 3%. It indicated that the material didn't degrade obviously. Conclusion Ethylene oxide sterilization is a better way for sterilizing absorbable PLLA screws.
7.Preventive effects of nitroglycerine on glucocorticoid-induced osteoporosis in growing rats.
Yuming, LI ; Yongguo, LI ; Weihong, YANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2007;27(5):528-31
The preventive effects of nitroglycerine (NG) on glucocorticoid-induced osteoporosis in growing rats were studied. Three-month-old female Wistar rats were randomly divided into control group (CON), dexamethasone group (DXM), DXM plus a low dose NG group (NG-L), DXM plus a middle dose NG group (NG-M) and DXM plus a high dose NG group (NG-H), 8 rats in each group. The rat model of osteoporosis was developed by intramuscular injection of dexamethasone twice a week. NG 0.2, 0.4 and 1.0 mg/kg was administered by oral gavages to the treatment groups every day for 12 weeks. Rats in CON group and DXM group were treated with normal saline of the same amount. After the treatment, the bone mineral density (BMD) and bone metabolism-associated biochemical markers were determined. Compared with CON group, BMD of lumbar spine and femur in DXM group was decreased significantly (P<0.05 and P<0.01 respectively), blood BGP levels and NO levels reduced (both P<0.01), and TRAP level increased (P<0.05). As compared with DXM group, BMD, serum BGP and NO were increased, and TRAP decreased in NG-L group and NG-M group, but had no significant difference in comparison to CON group. All the markers other than serum NO and TRAP levels had no significant difference between NG-H group and DXM group. It was concluded that low or middle doses of NG could prevent glucocorticoid-induced bone loss in growing rats, but high dose of NG could not. Supplement with NO donor could be considered as a preventive treatment for glucocorticoid-induced osteoporosis in a developing skeleton.
Bone Density/*drug effects
;
Dexamethasone
;
Nitric Oxide Donors/*therapeutic use
;
Nitroglycerin/pharmacology
;
Nitroglycerin/*therapeutic use
;
Osteoporosis/chemically induced
;
Osteoporosis/*prevention & control
;
Random Allocation
;
Rats, Wistar
8.Expression and purification of human cardiotrophin-1 in E.coli and identification of its biological activity
Zhengfeng ZHANG ; Weihong LIAO ; Qingfeng YANG ;
Journal of Third Military Medical University 2003;0(24):-
Objective To express recombinant human cardiotrophin 1 (huCT 1) protein with biological activity in E coli Methods huCT 1 open reading frame gene from plasmid pBSSK huCT1 was cloned into plasmid pMD18 T by PCR and T A cloning, and then cloned into prokaryotic GST fusion expression vector pGEX 2T to give pGEX2T huCT1 After pGEX2T huCT1 expression was induced by IPTG in E coli at different temperature and different time, the expression level and the proportion of the soluble protein were analyzed by SDA PAGE Then the soluble GST/huCT1 was purified by immobilized glutathione columns The GST fusion protein was cleaved by thrombin and purified again The recombinant huCT 1 with biological activity was identified according to the survival of axotomized sciatic motoneurons Results After the induction of pGEX2T huCT1 DH5? cells by IPTG at 29 ℃ for 4 h, the highest expression level of the recombinant protein was about 1/5 of total cell proteins, and the soluble portion was about 2/5 of fusion protein Purification of the soluble portion and thrombin cleaved fusion protein resulted in 85% and 80% purified recombinant GST fusion protein and huCT 1 protein, respectively Recombinant huCT 1 protected 55% motoneurons in spinal cord against sciatic axotomy in vivo in adult rats Conclusion Recombinant huCT 1 has biological activity in rat neurons
9.Laparoscopic Uterine Artery Occlusion Combined with Ascendant Myomectomy for Multiple Uterine Myomas
Weihong YANG ; Zhongping CHENG ; Hong DAI
Chinese Journal of Minimally Invasive Surgery 2005;0(10):-
Objective To evaluate the middle-term effect of laparoscopic uterine artery occlusion combined with ascendant myomectomy (LUAO-M) for multiple uterine myomas. Methods The uterine artery was isolated and occluded with Kleppinger bipolar forceps (Sabre 2400,ASPEN LABS USA) or PK forceps (Gyrus Medical Limited Inc UK) under a laparoscope. Then dissection was performed on the surface of pseudo capsule with Kleppinger unipolar needle (Sabre 2400,ASPEN LABS USA) or PK needle (Gyrus Medical Limited Inc UK),and the target myoma was stripped out of the tumor bed with the Separate-Scoop device. Afterwards,repair of the incision was carried out in one layer with interrupted single stitch by Absorbable VICRYL suture (Johnson VICRYL ETHICON USA). Results The mean operation time was (102?36) min and mean blood loss was (88.7?58.4) ml. The mean hospital stay after the operation was (7.9?0.2) d,and febrile morbidity was 5.1% (5/98). Complications included two cases of subcutaneous emphysema and one case of ileus;no other severe complications occurred. Of the patients,98 cases were followed-up for 21 to 52 months (mean,36.3 months),during the period they were visited by a mean of 3.6 times,which showed a correction rate of menstruation abnormality of 95.9% (4/98),rate of uterine volume reduction of 57.7%,and rate of recurrent myoma of 3.1% (3/98). Conclusion LUAO-M shows a good clinical outcome and middle-term effect for multiple uterine myomas.
10.Changes in dose requirements of rocuronium after right hemihepatectomy in donor for liying donor liver transplantation
Jie ZHOU ; Weihong YANG ; Xiangrui WANG
Chinese Journal of Anesthesiology 2009;29(7):585-587
Objective To investigate the changes in the dose requirements of rocuronium after right hemihepatectomy in donor of living donor liver transplantation. Methods Sixteen ASA Ⅰ patients of living donor liver transplantation, aged 21-49 yr, weighing 51-86 kg, scheduled for right hemihepatectomy, were enrolled in this study. Anesthesia was induced with iv midazolam and fentanyl, and TCI of propofol and rocuronium. Changes in adductor pollicis muscle were monitored by the train of four (TOF) stimulation. The patients were mechaincally ventilated after tracheal intubation when T1/Tc = 0. The target plasma concentration of rocuroniurn was reduced to 1.0 μg/ml after tracheal intubation and the concentration was regulated to maintain 0 < T1 ≤ 10%. Anesthesia was maintained with sevoflurane inhalation, TCI of propofol, intermittent iv injection of fentanyl. The infusion of rocuronium and inhalation of sevoflurane were stopped before the second time of cholangiography, the total time and amount of rocuronium administered and the recovery of muscle relaxation were recorded at this time (before hemihepatectomy). The infusion of rocuronium and inhalation of sevoflurune were rests-ted after right hemibepatectomy. After the peritoneum was closed, the infusion of rocuroniurn and inhalation of sevoflurane were stopped again, the total time and amount of rocuronium administered and the recovery of muscle relaxation were also recorded at this time (after hemihepatectomy). Results The amount of rocuronium administered was significantly reduced after hemihepatectomy compared with that before hernihepatectomy (P < 0.05). The amount of rocurunium administered after hemihepatectomy was (67 ± 13) % of that before hemihepatectomy. Conclusion Right hemihepatectomy can affect the metabolism of rocuronium in the donor of living donor liver transplantation.