OBJECTIVE:To explore the effects of atorvastatin combined with allopurinol on blood lipid and blood uric acid in patients with hyperlipidemia and hyperuricemia. METHODS:90 patients with hyperlipidemia and hyperuricemia were randomly di-vided into group A,group B and group C. Group A was orally given 0.1 g Allopurinol tablet,once a day+30 mg Atorvastatin calci-um tablet,once a day. Group B was given Atorvastatin calcium tablet(the same dose with group A). Group C was given Allopuri-nol tablet (the same dose with group A). The treatment course for all groups was 4 weeks. TG,TC,LDL,HDL,UA,BUN, ALT and CK before and after treatment,and incidence of adverse reactions in all groups were observed. RESULTS:After treat-ment,TG,TC and LDL in all groups were significantly lower than before,group A and B were lower than group C,the differenc-es were statistically significant (P<0.05);UA in all groups was significantly lower than before,group A and B were lower than group C,group A was lower than group B,the differences were statistically significant(P<0.05);ALT in group A and B,CK in all groups and BUN levels in group A and group C were significantly higher than before,CK in group A and group B higher than group C,group A was higher than group B,the differences were statistically significant(P<0.05). And there was no significant dif-ference in the incidence of adverse reactions among all groups(P>0.05). CONCLUSIONS:Atorvastatin combined with allopurinol can significantly reduce the blood lipid and blood uric acid levels of patients,with good safety.

Objective To explore the effect of hypoxia on cultured rat Sertoli cells in vitro.Methods In a Sertoli-germ cells co-culture system,the effect of hypoxia on the morphological characteristics of Sertoli cells were observed,including the number of Sertoli cells,the cytoskeleton changes after Coomassie brilliant blue staining and the detachment of germ cells.Results The loosing and retracting of cytoskeleton in Sertoli cells was found.Both survival rate of Sertoli cells and detachment of germ cells showed time-response relation.Conclusion Hypoxia damages Sertoli cells.

OBJECTIVE To investigate probationer nurses' knowledge about nosocomial infection (NI) and clean operating-room. METHODS Survey among probationer nurses was performed by self-designed questionnaires. RESULTS 47% probationer nurses thought it very necessary to strength knowledge about NI before probation. The scores of clean operating-room knowledge was lower than that nosocomial infection (NI); The cognition rate among high educated probationer nurses were higher than low educated,P

In 2010, the artificial synthesis of Mycoplasma mycoides triggers the new era of synthetic biology. This great breakthrough is achieved mainly thanks to the powerful DNA recombinant ability of yeast. In recent years, except for the methods used for large DNA assembly on the basis of in vivo homologous recombination, various different DNA assembly methods in vitro, based on the concept of DNA ligation or polymerization, have also been developed, such as Biobrick\BglBrick, SLIC and Gibson one-step assembly. Application of these new technologies has greatly accelerated the construction of synthetic part libraries, biosynthetic pathway and even microbial chromosomes. In fact, all DNA assembly methods are derived from the combinations of DNA joining and organizational schemes. This review describes the brief introduction of the main in vivo and in vitro DNA assembly protocols developed so for, which will benefit the construction of different types of synthetic functional devices and also biosynthetic pathways in the research of synthetic biology in China.
DNA ; biosynthesis ; chemistry ; genetics ; DNA, Recombinant ; biosynthesis ; genetics ; Genetic Engineering ; methods ; Metabolic Networks and Pathways ; Synthetic Biology ; methods

Objective To study the feasibility and safety for using anesthesia gastroscopy in aged-patients over 80 years old.Methods Selecting 84 cases of aged-patients over 80 years old as anesthesia group(group 1),22 cases of aged-patients over 80 years old as normal gastroscopy group(group 2)and 100 cases of young-middle age as anesthesia control group(group 3).Low dose propofol and fentanyl combined atropine were injected into venous in anesthesia gastroscopy.The BP,HR,SpO2,patients reactions during gastroscopy,stomach peristalsis rates,awake time were detected before,during and after gastroscopy.Results Patients in group 1 felt much less discomfortable than those in group 2.Anesthesia group felt much satisfactorily than normal gastroscopy group.The BP,HR,SpO2 in group 1 were decreased transitorily during examination and resumed rapidly after examination.There were no severe complications in anesthesia gastroscopy.However,the BP and HR were increased obviously in normal gastroscopy group.The dosage of propofol in group 1 was much less than that in group 3.Conclusions The use of anesthesia gastroscopy with low dose propofol and fentanyl combined atropine intravenously in aged-patients over 80 years old is safe and comfortable.

By analying the relationship between the construction of the periodical and the management of the medical quality, the author explained the positive effect of strengthening the former on level raising of the latter, She also proposes that the value of the periodical can be raised by the quality construction including superiority orientation and creativity development. In this way we will raise the additional value of the management of medical quality and provide a high-level information platform for the quality mangement of hospital.

Objective:To analyze the delay in the publication of medical postgraduates' research papers and provide them with a guidance for the management of academic articles.Methods:A total of 293 articles were chosen from the Journal of Medical Postgraduates published from 2005 to 2006,of which,229 were original articles and 45 were reviews,including 184 funded and 109 non-funded research papers,and their delay in publication in different periods was statistically analyzed by SPSS13.0 software.Results:The total delay in the publication of the articles was(234.7 ? 116.9) d,including(57.0?36.8) d in manuscript examination,(109.4 ? 96.4) d in editing and(61.8 ? 52.4) d in suspension.The original articles were less delayed than the reviews,(224.2 ? 7.4) d vs(272.8 ? 19.3) d,P

OBJECTIVE: To prepare compound ornidazole gel and conduct its quality control. METHODS: The gel was prepared with 0.6% carbopol - 940 as base and with ornidazole and cimetidine as main ingredients. Contents of 2 constituents were determined directely by UV - spectrophotometry. The stability of the finished product was investigated as well. RE-SULTS:The gel obtained was good in appearance, shaping and spreading. The linear ranges for ornidazole and cimetidine were all 0.5-32uuuuuuuuuug/ ml. The average recovery rates of ornidazole and cimetidine were 100.58% (RSD= 1.17%) and 100.59% (RSD=1.61%), respectively. The stability investigation results were in line with the requirement of Chinese Pharmacopeia 2000 version. CONCLUSION:The formula of compound gel is reasonable; its preparation technics is simple and the quality is reliable; and which meets the requirements of clinical medication.

OBJECTIVE:To evaluate the rationality of vancomysin use in the hospital where the authors work.METH-ODS:50patients taking vancomysin between May.2005and Nov.2005were studied.The pathogenic examination,monitoring of drug concentration in blood,indices of renal function,combined use of drugs as well as adverse effects in these patients were reviewed and analyzed statistically.RESULTS:On the whole vancomysin use in our hospital was safe.CONCLUSION:Vancomysin use will be made safer by monitoring drug concentration in blood,individualizing drug administration,and timely correcting the aggravating factors of kidney injury.

Objective To compare the short-term prognostic value of glycohemoglobin (HbA1c) and admission plasma glucose in non-diabetic patients with acute ST-elevation myocardial infarction.Methods Eighty-four non-diabetic patients with acute ST-elevation myocardial infarction from January 2010 to June 2011 were included.Both HbA1c and plasma glucose was measured on admission.Cardiovascular event was followed up in 30 days.Results The average of HbA1c and admission plasma glucose was as cut-off point.The patients were divided into HbA1c ＜ 5.5％ group (40 cases) and HbA1c ≥5.5％ group (44 cases) according to HbA1c level.The patients were divided into admission plasma glucose ≤ 8.6 mmol/L group (42 cases) and admission plasma glucose ＞ 8.6 mmol/L group (42 cases) according to admission plasma glucose.The incidence of cardiovascular event in 30 days in admission plasma glucose ＞ 8.6 mmol/L group was higher than that in admission plasma glucose ≤ 8.6 mmol/L group [19.0％ (8/42) vs.2.4％ (1/42)],and there was significant difference(P＜ 0.05).There was no significant difference in the incidence of cardiovascular event in 30 days between HbA1c ≥5.5％ group and HbA1c ＜ 5.5％ group (P ＞ 0.05).Admission plasma glucose showed weak correlation with blood creatine kinase isoenzyme MB peak (r =0.233,P ＜0.05).Conclusion In non-diabetic patients with acute ST-elevation myocardial infarction,elevated admission plasma glucose levels are associated with higher cardiovascular event in 30 days.