OBJECTIVE:To evaluate the effect of single decoction vs.mingled decoction on the content of Baicalin in Radix Scutellariae.METHOD:The separation of samples was performed on Agilent C18 column(250 mm?4.6 mm,5 ?m)with a column at room temperature.The mobile phase consisted of 0.5% formic acid-methano and 0.5% formic acid solution(55∶45)with flow rate of 1.0 mL?min-1 and detection wavelength of 274 nm.RESULTS:The linear range of Baicalin was 0.258 5～2.326 5 ?g(r=0.999 9)with an average recovery of 97.8%(RSD=1.1%,n=9).CONCLUSION:The content of Baicalin in Radix Scutellariae decocted alone is higher than in the mixture of all the single decoctions,and significantly higher than in Radix Scutellariae decocted by mingled decoction,all showing significant differences.

Objective To study the different influences of Xiaobanxiatang superfines (XBTs) made of Chinese medicinal materials and traditional decoction (XBTd) on gastrin. Method The model of mice were made with cisplatin. To observe the effect of the different forms of XBT on gastrin in serum and small intestine. Results After giving medicine, XBTd and XBTs remarkably increased the gastrin in serum and small intestine (P

By taking the evaluations made by scholars such as Wang Yongyan, et al. on status of TCM academic research as the introduction, various influencing factors in acupuncture school development are separately discussed, and the overall trend and existential state are described in modern acupuncture academic area. By displaying the opinions of acupuncture schools in recent years, the relationship among specific therapy, school and schools is analyzed to explain the similarities and differences of school and schools as well as their respective characteristics. It is proposed that the obstacles that lay before acupuncture school researchers are not the rights and wrongs of school and schools, but the evaluation criterion and development patterns that is appropriate for acupuncture research and development.
Acupuncture ; education ; trends ; Acupuncture Therapy ; methods ; trends ; Humans ; Moxibustion ; methods ; trends ; Teaching

Objective To observe the effective treatment of severe in peri-menopausal syndrome,and improve the quality of life of women in perimenopausall and postmenopausal women.Methods We review the different treatment methods in the 216 patients diagnosed with moderate to severe menopausal syndrome.Group A:simple hormone replacement therapy (HT) of 60 cases.Group B:simple treatment of traditional Chinese medicine of 90 cases.Group C:hormone replacement and comprehensive therapy of Chinese medicine of 66 cases.Follow up the improvement of symptoms and adverse reactions after the treatment one and three month respectively.Results The three groups after treatment in patients with clinical symptoms were improved after three month.Efficient comparisons among third group,the differences were statistically significant (90％ (54/60),82％ (76/90),100％ (66/66);x2 =13.160,P ＜ 0.01).Adverse reactions of 3 treatment group comparison difference had statistical significance (45％ (27/60,1％ (1/90),6％ (4/66);x2 =60.720,P ＜0.01).Compared with pure hormone treatment group,treatment group of Chinese medicine,comprehensive treatment group adverse reactions happen was less,the difference was statistically significant(P＜0.01).Chinese medicine treatment group and combined treatment group,there was no statistically significant difference (P ＞0.05).Conclusion Severe menopausal syndrome with hormone replacement therapy indications without contraindications suggestions take comprehensive treatment of traditional Chinese and Western medicine,The hormone supplement contraindications or unwilling to hormone replacement therapy,also has good effect of Chinese medicine treating.

Objective To summarize the main nursing point and experience of allogeneic small bowel transplantation.Methods One case underwent allogeneic small bowel transplantation in our hospital on the first of December 2010,the nursing measures were summarized.Results The patient passed the operation well-off,and survived the infection phase and repulsion phase in the transplantation ward.Conclusions Valid preoperative evaluation,taking active measures to prevent intraoperative hypothermia and infection,using self-made vacuum pads to prevent pressure sores during operation,are the guarantees for successful operation.

Objective To explore the difference of hand hygiene requirement between guide-line of skill practice for national examinations of doctors' qualification(clinical practitioners) and stan-dard for hand hygiene for healthcare workers in health care settings for some sterile techniques. Methods Eight sterile techniques in guideline of skill practice for national examinations of doctors' qualification(clinical practitioners) such as changing a dress and taking out stitches, aspiration, ure-thral catheterization, arteriovenous puncture, thoracentesis, abdominal puncture, lumbar puncture and bone marrow puncture were chosen and the results were compared with standard for hand hygiene for healthcare workers in health care settings. Results Three of eight sterile technics mentioned hand-washing, six of eight mentioned wearing sterile gloves, and zero of eight mentioned antiseptic han-drubbing; however, only one of six (16.67%) before wearing sterile gloves mentioned handwashing. Conclusion The requests of hand hygiene between guideline of skill practice for national examina-tions of doctors' qualification (clinical practitioners) and standard for hand hygiene for healthcare workers in healthcare settings are not concordant , which from the root leads to the inconsistence of doctors' learning at different stages of education and training . It is suggested that the examination organization departments should refer to the relevant provisions of the state in the preparation of guidelines, and regulate medical practice behavior with consistent standards.

C_(57)BL/G male mice were immunized with SRBC 9 hr after 75 mGy whole body X-irradiation. The hypothalamic M-Enk and L-Enk contents were decreased. Serum TS level kept on fluctuating over the control level. The change of testicular cAMP content was not in the same direction as that of the TS. The change of serum CS level was opposite to that of serum TS, These results suggest that the low dose radiation probably cause the changes of neuroendocrine function, leading to the decrement of hypothalamic EOP contents, the supression of CRF-ACTH-CS axis activity and the enhancement of GnRH-LH-TS axis activity.

The present study has demonstrated a lowered reactivity to Con A stimulation, and also a decreased IL-2 production by splenocytes in adult male rats which were thymectomized within 48 h after birth. Meantime, their body weights, pituitary and testicular weights, serum LH and FSH levels, serum and urine TS contents, and pituitary cAMP contents were all lower than that of the control. The hypothalamic 5-HT metabolism showed that a change of hypothalamic-pituitary-gonad axis might have occured after neonatal thymectomy in male rats.

The recent advances in research of oxygen free radicals and its relation to Parkinsons disease (PD) was reviewed. Though there is no exact knowledge of the causes of PD, the role of oxidative stress in the etiology of PD has been focused on. Investigations on patients have shown that PD is under a status of oxidative stress. The biochemical features of dopaminergic neurons in the pathologic site of PD implicate the close relation of oxygen free radicals' generation with the pathogenesis of PD. Besides the oxidative stress hypothesis, other proposed hypotheses are also direct or indirect related to oxidative stress.

Objective To study the relationship between serum Estradiol(E2 ) andβ-amyloid protein(β-AP) in patients with cer-ebral infarction .Methods A total of 46 female patients with cerebral infarction were selected from 2011 January to 2014 June into observation group ,46 cases were divided as 20 cases in mild group ,16 cases in moderate group ,10 cases in severe group on the basis of neural function defect score standard ,according to the size of infarct volume 46 patients were divided into small infarct volume group with 21 patients ,moderate infarct volume group with 14 cases ,larger infarction volume group with 11 cases .In the same peri-od ,64 healthy women were recruited into control group .Serum E2 ,β-AP levels of serum and cerebrospinal fluid were detected and compared ,and the relationship of serum E2 andβ-AP level in serum ,cerebrospinal fluid were analyzed .Results Theβ-AP levels in serum and cerebrospinal fluid in observation group were higher than those of control group ,the serum E2 level was significant lower than that of the control group ,the differences were significant(P<0 .05) .With more severe cerebral infarction ,β-AP levels in serum cerebrospinal fluid showed an upward trend ,with more severe cerebral infarction ,the serum level of E2 was significantly decreased , andβ-AP ,E2 levels in mild ,moderate ,severe group had significant differences(P<0 .05) .Theβ-AP levels of serum and cerebrospi-nal fluid in higher infarct group were higher than those in small ,moderate infarction group ,but E2 levels were lower than those in small ,moderate infarction group ,the differences had significant(P<0 .05) .There were no significant difference inβ-AP of serum and cerebrospinal fluid ,Serum E2 level between small and moderate infarction group(P>0 .05) .The expression of serum E2 andβ-AP in serum and cerebrospinal fluid were negatively correlated(r= -0 .428 ,P=0 .009;r= -0 .476 ,P=0 .005) .Conclusion β-AP levels in serum and cerebrospinal fluid in patients with cerebral infarction are high ,closely related with the severity of cerebral in-farction .β-AP levels of cerebrospinal fluid ,serum in cerebral infarction patients are negatively related to the level of serum E2 .