Objective To investigate the composition and drug resistance of pathogenic bacteria of nongonococcal urethritis in men,in order to give some references for clinical treatment.Methods The pathogens were cultured by Urethral secretions of nongonococcal urethritis in men and drug susceptibility test followed.Results Coagulase negative staphylococcus and mycoplasma urealytium were major pathogens of nongonococcal urethritis in men;The constituent ratio of meticillin resistance staphylococcus was above 40%.There was significant difference that the constituent ratio of meticillin resistance coagulase negative staphylococcus compared with specimen in the same period.Meticillin susceptible coagulase negative staphylococcus and meticillin susceptible Staphylococcus aureus were sensitive to cephalosporins and quinolones,excluding penicillins.The antimicrobial susceptibility of meticillin resistance staphylococcus was just opposite completely;Mycoplasma urealytium and Mycoplasma hominis were high sensitivity to josamycin、doxycyline and minocycline.Conclusion The colonization bacterium of nongonococcal urethritis in men are primarily consist of coagulase negative staphylococcus and mycoplasma urealytium.It is worthy of further researching correlation between coagulase negative staphylococcus and nongonococcal urethritis,it also suggested that antibiotics on should be rationally choosed on therapy of nongonococcal urethritis.

Endothelial cells can be activated, and develop dysfunction and damage under the pathological state. Endothelial dysfunction plays a key role in the processes of cardio-cerebrovascular diseases, and can be detected in peripheral blood. The concentration of von Willebrand factor (vWF) predicts the risk of coronary heart disease and stroke; the concentration of soluble thrombomodulin is associated with the prognoses of coronary heart disease and stroke; soluble intercellular ashesion rnolecule-1 (ICAM-1) predicts the risks of carotid athero-sclerosis, coronary heart disease, peripheral arterial disease, and diabetes mellitus; the levels of plasma soluble E-selectin in patients with carotid atherosclerosis, coronary artery disease, and diabetes mellitus are increased; the increased endothelin-1 levels predicts the elevated risk of congestive heart failure. The injection of endothelial progenitor cells is promising to become a novel approach for the treatment of cardio-cerebrovascular diseases.

Objective: To study the cloning, recombinant expression and purification of human HSP70D. Methods: The full length code region of HSP70D was amplified by RT PCR from HeLa cells. Then the PCR product was inserted into pET 24a(+), by NdeⅠ/XhoⅠ double digestion. The recombinant expression vector pET24a HSP70D was transformed into E. coli strain BL21. Positive clones were determined by NdeⅠ/XhoⅠ digestion and sequence analysis, which was used in the expression and purification of HSP70D protein. The engineered bacteria were collected after fermentation. The recombinant HSP70D was purified by using ethanol precipitation, anion exchanger, hydrophobicity interaction chromatography, and finally high resolution gel filtration chromatography. Results: The expression level of HSP70D was over 20% of total protein in the engineered bacteria. After purification, electrophoretically pure (more than 95%) rhHSP70D was obtained. Conclusion: This study setup a satisfied down stream process which can be used for production of large quantity of recombinant HSP70D.

Ten cats lightly anesthetized and D-tubocurarine immobilized were employed.The responses of a same unit to the train stimuli of square wave applied to the saphenous nerve,the greater splanchic nerve,and the two nerves simultaneously were observed.Among the 80 units recorded on the 10 cats,43 went through all the experimental procedures,33 out of the 43 could be activated by splanchic inputs.Convergence of the splanchic and saphenous inputs occurred to 26(79％) out of the 33.These 26 units could respond to the noxious stimuli on the saphenous and greater splanchic nerves in turn or simultaneously.Furthermore,they could be divided into 3 types;15 units showed similar pattern and duration of discharge only when the greater splanchic nerve was stimulated,6 showed similar discharge only when the saphenous nerve was stimulated,and 5 showed smaller responses when either of the two nerves was stimulated.When the interval between stimulations was shortened to 5 seconds,37(86％) out of the 43 units showed a decrease in discharge,which was apparently an after-inhibitory effect.The results indicate that the inputs from visceral and somatic C or A-delta afferent fibers can converge onto the units of the dorsal hippocampal CA1 area,and evoke blockage and inhibition of these units.

Objective: To determine the adjuvant activity of recombinant human HSP70D protein. Methods: Dendritic cells were cultured in vitro , rhHSP70D was added into the culture medium, the cytokine secretion and maturation of dendritic cells(DC) stimulated by rhHSP70D were measured; C57BL/6 mice were immunized with rhHSP70D OVA 257 264 complexs reconstituted in vitro , and the protective antigen specific immune response against tumor challenge were evaluated in the immunized mice. Results: rhHSP70D stimulated human DC to secrete IL 1?, IL 12 p70 and TNF ?, up regulated the HLA DR,CD86 and CD40 expression on DC and improved the antigen presentation function of DC; After immunization with the hybrid of rhHSP70D and OVA 257 264 , the mice were able to resist the challenge of E. G7 OVA, which was a tumor cell line expressing model antigen OVA. Conclusion: rhHSP70D can induce maturation of DC, immunization of rhHSP70D peptide complexs can induce antigen specific immune response, suggesting that rhHSP70D may be a new adjuvant in immunotherapy of the cancer and infection diseases.

Popularization of the computer and multimedia in the teaching management,calls for the new demand of the teaching information storage.When we widely use the new technology,we should pay attention to the storage of the electronic information,which should be stored as papers in case of the loss caused by the technology or equipment problems

This article discusses the important effect of language techniques in the pharmacology teaching,according to the peculiarity of this speciality and the experience of author,and the concrete language techniques to activate the class to attract the students' attention and to improve teaching effect from the aspects of the basic request of language expression in class,the scientificity and the logicality and the enlighten and techniques of teaching language in pharmacology class.It also explains the importance of language techniques in the pharmacology teaching from many aspects.

With the reformation of university education system,cultivating technological talents and creative talents is proposed,so the laboratory teaching in sports anatomy reformation is imperative.The laboratory teaching in sports anatomy is based on anatomical knowledge,relied on the sport practice.Its goal is to solve the practical problem in P E Work.At present,experimental class hour is limited and the experiment content does not accord with its major,and the experiment examination method is not reasonable.This paper puts forward reforming the experimental class hours,changing experiment items and reforming the examine method,which will drive the innovation of sports anatomy course system

Objective To investigate the effect of health education in the community of high risk group of pressure sore , and to provide a reference for the formulation of community health education strategies .Methods 148 subjects with NORTON score discharge assessment results less than or equal to 14 points were randomly divided into observation group and control group with 74 cases in each group.The control group received routine discharge instructions.The observation group in the conventional discharge guidance on the implementation of the home, regular and telephone guide.The self-made pressure ulcer prevention knowledge questionnaire were com-pared between the two groups at the time of discharge and intervention for caregivers to grasp the situation of pressure ulcer prevention knowledge after 3 months;The incidence rate of discharged patients in the two groups after the comparison of pressure ulcer were com-pared.Results After the intervention, the observation group significantly increased the pressure ulcer prevention, which was signifi-cantly higher than before the intervention and the control group after intervention (all p<0.05);In the observation group was discharged after 3 months, 6 months and 9 months were no bedsore.Control group 3 months after discharge pressure sore the incidence rate was 8. 11%.6 months after discharge pressure ulcer incidence rate was 16.22%.9 months after discharge pressure ulcer incidence was 22. 97%.The observation group of 3 months, 6 months, 9 months after hospital discharge rate of pressure ulcer was significantly lower than that of control group (all p<0.05).Conclusion Health education is helpful to improve caregivers knowledge related to pressure ulcer prevention, reduce the risk of high-risk population community bedsore, and is worthy of popularization and application.

The purpose is to introduce the structure and principle of the high intensity focused ultrasound and to discuss its value and future development in tumor. The high intensity focused ultrasound uses the biological heat effect which focuses the high intensity ultrasound on the lesions to destroy them. It is non-invasive means to destroy the lesions, reduce the damage of the normal tissues greatly, improve the quality of life..With the development, application and popularization of the technology on high intensity focused ultrasound, it will serve the human health career better.