AIM: To compare two methods of HPLC-MS/MS and MEIA to monitor the tacrolimus concentrations for organ transplantation patient.METHODS: After developing and validating the HPLC-MS/MS method to determine the concentrations of tacrolimus,the tacrolimus samples were quantitated by two methods of HPLC-MS/MS and MEIA,respectively.Evaluate two methods by statistical analysis of quantitative results.RESULTS: The mean concentration were(4.86?0.46) ng/mL by HPLC-MS/MS and(5.52?0.43) ng/mL by MEIA.The coefficient correlation of two methods was 0.8771,and the two methods have good correlations in statistics.The mean concentration ratio was(90.3?5.3)%.CONCLUSION: The HPLC-MS/MS method is a more precise method for to determining the effective concentration of tacrolimus,which is suitable for daily TDM.

Objective To discuss the surgical technique and effectiveness of total knee arthroplasty (TKA) for the valgus knee in adult.Methods Retrospective analysis the clinical data from 2015 January to 2017 May of 38 patients with 40 knees treated in our hospital.Age 46-73(61 ±4.2) years old.The femur-tibia angle (FTA) was 7°-35 ° (11 ° ± 4.3 °),the average range of motion (ROM) was 115 ° ± 10°,the Hospital for Special Surgery (HSS) score was 21-65 (43 ± 8.6).All cases were corrected deformity and improved function by TKA.Results 38 patients were followed up 6 months to 3 years.All patients had no severe pain and dysfunction.The postoperative FTA was 1 °-7°(4 ± 3°),the average ROM was 115° ± 10°,the HSS score was 76 ± 14,showing significant difference compared with preoperative data (P ＜ 0.05).Conclusions The TKA is a conventional therapy for valgus deformed knee in adult.By appropriate approach,precise osteotomy,accurate soft tissue balance,satisfactory joint function and effectiveness can be achieved.

Objective To research clinical application of rotating platform knee prosthesis Gemini MK II in the total knee arthroplasty. Methods From August 2010 to July 2013, 87 patients (106 knees) underwent total knee arthroplasty using rotating platform knee prosthesis Gemini MK II. The average ages of the cases were 65.3 years (52-81 years). The clinical results were evaluated by the HSS knee rating score system as well as X-ray films. Results The patients were followed up for mean 24.2 months (7~40 months). The pain caused by the illness of knee obviously alleviated and the function of knee significantly improved. The complications such as osteolysis around the prosthesis, prosthesis loos-ening and dislocation of meniscus bearings were not found. The HSS knee rating score was (51.5±9.7) before total knee arthroplasty, which improved to (87.3±6.4), The difference was significant(P<0.01). Conclusion There are satisfactory results in a short time for rotating platform knee prosthesis Gemini MK II in the total knee arthroplasty. A long term follow-up is obviously necessary to make more precise statement about the knee replacement system.