The common, particular structure and the arrangement pattern of the retinula in the compound eye of the housefly Musca domestica have been investigated, and the structures of the retinula cells here been compared with an electron microscope under dark, bright and high intensity light adaptation condition.In general, the retinula of the compound eye is composed of eight retinula cells, but some retinula is composed of six, seven, or nine, ten retinula cells.The arrangement pattern of the retinula in the right, left compound eye and dorsal, ventral regions of the compound eye are the mirror image inversion. But the arrangement pattern of some retinula in the dorsal or ventral region is also the mirror image inversion.The greater part of pigment granules in the retinula cells No.1～6 move towards the rhabdomere, the pigment granules in the retinula cells No.7～8 do not move, but the pigment granules in the some retinula cells No.7～8 move towards the rhabdomere under high intensity light adaptation condition.

1. The central retinular cells R_7 in the compound eye of male housefly can be divided into two types which are called here R_(7a) and R_(7b) respectively. R_(7a) approximate many features of R_(1-6) cells, which have a larger, cell body, rhabdomere and axon, terminate in the first optic neuropil (lamina). R_(7b) approximate many features of R_8 cell and project directly to the second optic neuropil (medulla). 2. The central retinular cell R_(7a) in the compound eye of male housefly distribute mainly the dorsal region of the eye, there are a few of R_(7a) in the ventral region of the eye, which only distribute in the first and second row ommatidia under equatorial line, but not found in the compound eye of female housefly.

Objective To study and find the reason and treatment way of severe encephalocele after operation on the brain. Me thods 15 cases who have severe encephalocele were reviewed retrospec tively.Results Among 15 cases, 12 cases have been cured,3 c ases died with severe pulmonary infection.Conclusion The re asons of severe encephalocele are hydrocephalus?cerebral edema and intracranial infection and it is useful to utilize medicine and other ways to lighten hydroc ephalus?cerebral edema and intracranial infection for the patients who have sev ere encephalocele.

Objective To study the expression of thymus and activation-regulated chemokine(TARC)in the kidney of MRL/lpr mice and to elucidate the molecular pathogenesis of lupus nephritis.Methods The 24-hour urine protein of 4 MRL/lpr mice of 16 weeks of age and 4 age-and sex-matched BALB/c mice was measured.The expressions of TARC mRNA and protein in the kidneys of MRL/lpr mice and BALB/c mice were detected by reverse transcription polymerase chain reaction and Western blot,respectively.Results The 24-hour urine protein and the expression level of TARC mRNA were significantly higher in MRL/lpr mice than in BALB/c mice(P＜0.05).The expression of TARC protein was detectable only in MRL/lpr mice.Conclusion The expression of TARC increases in the kidney of mice with systemic lupus erythematosus,suggesting that TARC may be involved in lupus nephritis in mice.

Objective To investigate changes of T lymphocyte subsets in peripheral blood and ascitic fluid in patients with tuberculous peritonitis and explore changes of systemic and local immunological function of patients.Methods The levels of T lymphocyte subsets in peripheral blood and(or) ascitic fluid were detected by flow cytometer in 38 cases of tuberculous peritonitis before and after therapy, 29 cases of cirrhosis of liver complicated with ascites formation and 15 cases of normal subjects as control group.Results ①The levels of T lymphocyte(CD 3 +), helper/inducer T lymphocyte(CD 4 +)and helper T lymphocyte /suppressor T lymphocyte(CD 4 +/CD 8 +)were obviously decreased in patients of tuberculous peritonitis,as compared with normal subjects(P

Objective To evaluate the effectiveness of locking compression plate (LCP) in treatment of comminuted fractures of distal end of radius. Methods A retrospective analysis of the clinical data was done for the 24 patients with comminuted fracture of distal end of radius who had been treated by locking compression plate fixation from August 2002 to August 2003. Results The follow up duration averaged 7.8 months. The satisfactory rate of functional outcome with LCP fixation was 91.6 percent. Conclusion Although LCP can provide the outstanding stability theoretically, it has not resulted in more exciting outcomes in treatment of comminuted fractures of distal end of radius than traditional methods.

Objective To detect the positivity of circulating autoantibodies against cardiac troponin Ⅰ (cTnI) in acute myocardial infarction (AMI) and myocarditis patients and investigate the interference of the antibodies with the detection of cTnI. Methods cTnI ELISA was established for assessment of sera obtained from 121 patients with AMI, 24 with myocarditis and 210 healthy subjects. Binding specificity of cTnI antibody from positive sera by ELISA was confirmed with Western Blot. The recovery of cTnl studies was employed to evaluating the effects of cTnl autoantibodies on cTnI immunoassays. Results Thirteen of the 121 AMI patients (10.74%) and 2 of the 24 myocarditis patients (8.3%) had positive anti-cTnI antibodyas compared with none in the healthy subjects. The recovery of cTnI by adding cTnI-C fusion proteincorresponding to final cTnI concentration of 0.625-100 ug/L to sample with anti-cTnI antibody wasinhibited significantly (Spearman correlation coefficient r=0.943, P=0.005 ). There was no significantchange of recovery of cTnl when adding it to a normal sera(Spearman correlation coefficient r=0.377,P=0.461). When cTnI-C complex corresponding to 20 ug/L cTnI was added, 5 of the 15 sera with anti-cTnIantibody were found with the inhibition of recovery (<80%). Conclusions Autoantibodies against cTnIwere increasing in patients with AMI and myocarditis, yet it appears that these autoantibodies could interferenegatively with the cTnI immunoassay. Thus, it should be paid more attention to cTnI immunoassays.

Objective To study the relation of type Ⅱ topoisomerase gene mutation and mechanism of Ureaplasma urealyticum resistant to quinolones. Methods 13 isolates of Uu resistant to 6 quinolones were selected from 184 clinical isolates by using broth dilution method, and their gyrA, gyrB, parC, parE were amplified by PCR.After sequencing, results were compared with the nucleotide sequence of susceptible reference strain. ResultsMICs of resistant Uu isolates were 4 to 32 fold higher than susceptible reference strain counter parts; sequence comparison revealed a C to A change at 87nt of gyrA QRDR led to the substitution of aspartic acid by glutamic acid and a C to T change at 50nt of parC QRDR led to the substitution of serine by leucine, with no amino acid change observed in GyrB and ParE. Conclusion These results suggest that a C to A change at 87nt of gyrA QRDR and a C to T change at 50nt of parC QRDR are associated with quinolone resistance of Uu.

Objective To investigate angiographic manifestations and interventional treatment of renal arteriovenous fistulas. Methods Renal arterial angiographies were performed in 20 patients from multiple hospitals.These patients suffered from repeatedly intermittent hematuria,but ultrasound,KUB,IVU,CT and endoscopy could not contribute to the diagnosis.The angiographic manifestations and treating procedure were analyzed. Results All the 20 cases were diagnosed as renal arteriovenous fistula by means of angiography.According to the image manifestations,these cases were classified into three types:(1)deformed branches and shunt in 11 cases;(2)high discharge shunt without deformed banches in 4;(3)concealed shunt showed by superselective segmental artery angiography but negative by renal artery angiography in 5.All fistulas of the 20 cases were successfully embolized with different materials including gelfoam,sodium morrhuate,ethanol,PVA,TH-glue,coils,etc. Conclusions Superselective renal angiography and embolization are the most important and effective tools for diagnosis and treatment of renal arteriovenous fistula.

[Objective]To investigate the treatment of nonunion after Pilon fracture with fibula approach fixation of tibia and ankle joint fixation and its clinical efficacy.[Method]Six cases of nonunion after Pilon fracture were collected from 1999 to 2004,which included 4 males and 2 females,with the average age of 34.7 years(21~53 years).All fractures were found invasion to the tibia-fibula joint and ankle malformation.Fibula and tibia were opened from posterolateral approach and reconstruction plate was placed laterally on the fibula,with screws crossing the fibula into the tibia directly.Autogenous bone graft was used in fracture position and area between tibia and fibula to gain tibia-fibula fusion.[Result]All cases were followed up from 8 months to 4 years with the average of 22 months.Bone fusion were gained in 5 cases with the average time of 3.5 months.Plate broken was found in 1 case for too early weight bearing.[Conclusion]Fibula approach fixation of tibia and ankle joint fixation has advantages of promoting fracture healing,preventing complications,and correction joint malformation.It is a convenient and reliable method for clinical surgeons.