0 05) Observation group was much better than control group in decreasing levels of TG and Ch(P

Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) has become an indispensable tool in macromolecule and small molecule analysis for solid and liquid samples.However, there were few studies focusing on the ionization mechanisms especially for the small molecules.In this work, the compounds of pyrene, coronene and rubrene which have similar molecular structures were used to investigate the ionization mechanism via SALDI-MS.Additionally the effect of the laser power on the product ions was also investigated.It was found that strong cluster ions peaks nM+(M=pyrene, coronene) were observed for pyrene and coronene, and daughter ions of coronene by loss of C2H2 were also observed.On the contrary, no cluster ion was obtained for rubrene, only daughter ions with the losses of nC6H5 (n=1, 2, 3) were acquired.Finally, the ionization mechanisms were discussed.The formation of clusters of pyrene and coronene was attributed to the interaction of π-π bands.For rubrene, the spatial barrier weakened the interaction of π-π bands because the four phenyl groups were not on the same plane of skeleton structure, thus impeding the formation of cluster ions.

Objective To discuss the efficacy of Lamiophlomisrotata Dripping Pill combined with intra-articular injection of medical ozone in the treatment of knee osteoarthritis.Methods Eighty patients with knee osteoarthritis were selected,the control group (39 cases) were administered Lamiophlomisrotata Dripping Pill,the observation group (41 cases) were administered Lamiophlomisrotata Dripping Pill combined with intra-articular injection of medical ozone.The efficacy of Lamiophlomisrotata Dripping Pill combined with intra-articular injection of medical ozone in the treatment of knee osteoarthritis were evaluated by efficacy,knee function,and inflammatory factor.Results After treatment,the effective rate of the observation group was higher than that of the control group (P ＜ 0.05).In the observation group,there were 38 cases of patients who felt pain,tenderness,and swelling symptom relief.There were 28 cases in the control group.After treatment,the scores of pain,activity,walking ability,and KSS in the observation group were higher than that of the control group (P ＜ 0.05).There was no statistical significance on the stability between two groups.Before treatment,there were no statistical significance on the IL-6,IL-8,and TNF-α.After treatment,the IL-6,IL-8,and TNF-αt were increased in two groups (P ＜ 0.05).And these indexes in the observation group were higher than that of the control group (P ＜ 0.05).Conclusion In summary,Lamiophlomisrotata Dripping Pill combined with medical ozone had good effect on the knee osteoarthritis.It could release the pain,improve knee function and anti-inflammatory.It was worthy of clinical use.

Objective To observe the sedative effect of dexmedetomidine in senile cataract surgery with retrobulbar nerve block.Methods Ninety-three senile cataract patients,ASA Ⅱ or Ⅲ grades,aged from 65 to 82 years old,scheduled for cataract surgery with retrobulbar nerve block were equally randomized into three groups by systematic sampling.The patients in three groups were given bolus infusion dexmedetomidine 0.5 μ g/kg before operation and group A was maintained by 0.2 μ g/ (kg· h),group B was maintained by 0.4 μ g/(kg· h) and group C was maintained by 0.6 μ g/(kg· h).Ramsay score,mean arterial blood pressure (MAP),heart rate (HR),respiratory rate,pulse oxygen saturation (SpO2) were recorded before anesthesia (T0),after retrobulbar nerve block (T1),and after sedative administration for 10 (T2),20 (T3),30 (T4),60 min (T5).Results The operation in three groups was successful.The scores of Ramsay at T2-T5 in three groups was significantly higher than that at T0 [group A:(2.6 ± 0.7),(2.5 ±0.2),(2.4 ±0.8),(2.4 ± 0.3) scores vs.(2.0 ± 0.0) scores;group B:(3.0 ± 0.7),(3.8 ± 0.7),(4.2 ± 0.2),(4.5 ± 0.2)scores vs.(2.0 ± 0.0) scores;group C:(3.8 ± 0.6),(4.7 ± 0.4),(5.2 ± 0.8),(5.6 ± 0.6) scores vs.(2.0 ± 0.0) scores].The scores of Ramsay at T3-T5 in group C was significantly higher than that in group A and group B,the scores of Ramsay in group B was significantly higher than that in group A,there was significant difference (P ＜ 0.05).The HR at T3-T5 in three groups was significantly lower than that at T0 [group A:(77 ± 5),(76 ± 7),(74 ± 12) times/min vs.(88 ± 12) times/min; group B:(72 ± 9),(70 ± 8),(70 ± 11)times/min vs.(90 ± 10) times/min;group C:(64 ±7),(55 ±7),(54 ±6) times/min vs.(88 ±9)times/min],the HR at T3-T5 in group C was significantly lower than that in group A and group B,there was significant difference (P ＜ 0.05).The level of MAP,respiratory rate in three groups and interclass had no significant difference (P＞ 0.05).There was no respiratory depression,nausea,vomiting and dizzy occurred.Conclusion Bolus infusion dexmedetomidine 0.5 μ g/kg followed by intravenous infusion at 0.2-0.4 μ g/(kg·h) is suitable for sedation of elderly patients with cataract surgery.

It is proved that there are significant differences in individual susceptibility to lung cancer.Genetic polymorphisms of several enzymes involved in the detoxification and biotransformation might be related to the lung cancer susceptibility.Cytochromes P450,N-acetyltransferases,microsomal epoxide hydrolase,NQO1 are the most frequently investigated genes in recent years.All theses genes appear to be the candidates for lung cancer susceptibility genes.The studies of the relationship between polymorphisms of metabolic enzymes and lung cancer susceptibility will help to find useful biomarkers.An improved understanding of genetic polymorphisms may help to identify individuals who are at increased risk for lung cancer and decrease the incidence of lung cancer.

BACKGROUND:Bony and structural feature often cause pulout strength decrease of pedicle screw, which induces loosening and pulout, and finaly results in fixation failure. Thus, it is very important to elevate the stability of pedicle screw.
OBJECTIVE:To detect the biomechanical stability of bone cement injectable canulated pedicle screw, and to provide reference for bone cement dosage.
METHODS: We selected T11-L4 samples of seven fresh adult corpses, containing 40 vertebral bodies. They were randomly divided into bone cement injectable canulated pedicle screw group and DTPSTM pedicle screw group (n=20). After screw implantation, 1, 2, 3 and 5 mL bone cement was injected. The diffuse distribution of bone cement was observed by imaging. The maximum axial pulout strength was measured.
RESULTS AND CONCLUSION:When the dose of bone cement was 1-3 mL, the average maximum axial pulout strength was significantly greater in the bone cement injectable canulated pedicle screw group than in the DTPSTM pedicle screw group (P < 0.05). When the bone cement dosage was 5 mL, no significant difference in the maximum axial pulout strength was detected between the two groups (P > 0.05). The regression equation was Y=25.269X+133.681 (R2=0.837) in the bone cement injectable canulated pedicle screw, and Y=32.039X+99.251 (R2=0.936) in the DTPSTM pedicle screw group. When the dosage of bone cement was 1-5 mL, the maximum axial pulout strength was highly positively correlated with bone cement dosage (|R| > 0.8). These results suggested that bone cement augmentation pedicle screw could apparently elevate the stability of the screw. The maximum axial pulout strength of the pedicle screw was positively correlated with bone cement dosage. After reaching the satisfactory fixation effects, the bone cement injectable canulated pedicle screw can reduce bone cement dosage, diminish the risk of bone cement leakage, and have more advantages than DTPSTM pedicle screw.

Objective To explore the application of near infrared fluorescent dye C-dots in cell labeling.Methods B16 melanoma cells were cultured with C-dots of various diameters.The distribution of C-dots in cells was observed by laser confocal fluorescent microscope.The proliferation of both C-dots-labeled and unlabeled cells were measured by MTT colorimetry.The viability of labeled cells was checked by trypan blue staining.Results C-dots with diameter of 5 or 10 nanometers can be used to label cells.The near infrared fluorescence of C-dots was taken into the cells and observed on the cellular membrane.No difference of cellular viability was found between the labeled and unlabeled cells.Conclusion C-dots can be used as an intracellular marker for the research on trace and proliferation of cells.

With growing dependence on picture archiving and communication systems(PACS) for viewing images,a quality assurance program to the condition of workstation displays and image quality guidelines for better cost-efficacy and diagnostic accuracy has become increasingly important.This article analyses and synthesizes the quality control of the PACS phantom workstation mainly from such aspects as the monitor resolution and brightness,the environment degree of illumination,statistics assessment method(ROC curve) and so on.

Objective To evaluate the mid-term outcomes of endovascular repair of descending thoracic aortic aneurysm(DTAA) in high-risk patients.Methods Twenty four patients with DTAA received stent-grafts implantation between January 2001 and July 2007 and their clinical data were retrospectively analyzed.All patients were considered high-risk for open surgical repair due to the location or rupture of the aneurysm,high age and severe comorbidities.Spiral computed tomography angiography and 3-dimentional reconstructions were performed at 1,3,6 and 12 months postoperatively and thereafter every year.Results The primary operational success rate was 100%.There were 2 deaths(8.3%) during perioperative period due to multiorgan failure and acute myocardial infarction respectively.Two(8.3%) patients had severe post-operational complications(1 stroke and 1 acute renal insufficiency).Eleven cases of type Ⅰ(46%) endoleak were detected immediately after the operation and among them,the endoleak disappeared after balloon dilatation in 3 cases while the other 8 patients were treated conservatively.Follow-up of 1 to 60 months(mean 18.6?4.2 months) was completed in 19 patients(79.2%).Stent displacement with type Ⅰ endoleake was found in 1 patient at 4 years after the operation and delayed type Ⅲ endoleak occured in another patient at 2 years post-operation.Both patients received endovacular repair again and the endoleaks were closed successfully.One patient died of colon cancer during the follow up.Complete thrombosis of the thoracic aneurysm sac and no stent migration or endoleak was found on the followup CT at 3 months after the operation in all the patients.The decrease in maximal aneurysm diameter was 0-18 mm(mean 6.3?3.1 mm).The prosthetic vascular grafts implanted in 4 patients with preliminary carotid subclavian bypass surgery were patent during the follow-up period.Conclusion Treatment of descending thoracic aortic aneurysm in high-risk patients with endovascular approach showed acceptable early mortality and morbidity and may be considered as a treatment alternative for carefully selected patients.

Objective To observe the anatomic landmarks in the thoracic cavity under thoracoscope in procedures for scoliosis,so as to facilitate the intraoperative localization and the prevention of complications. Methods During 23 cases of thoracoscopic anterior procedure,anatomic landmarks,including the segmental vessels,azygous vein,intervertebral discs,rib heads and great splanchnic nerve,were observed. Results The segmental vessels of T 6～10 were situated at the center of the vertebral body and their position was relatively constant.The T 7～10 intervertebral discs were a little bit prominent and white-colored,which were easily distinguished.The rib heads corresponding to their vertebral bodies were good landmarks.The great splanchnic nerve crossed over the T 7～9 vertebral bodies arrisways. Conclusions With the assistance of anatomic landmarks,accurate localization of the vertebral body is possible,which is helpful to the prevention of spinal cord injury and the decrease of frequency of radiation exposure.