Objective To study the MRI features of normal and abnormal axillary lymph nodes so as to explore the evaluation of MR breast coil on metastasis of axillary lymph nodes in patients with breast cancer before operation.Methods MR scanning was made on 119 axillas from 24 healthy volunteers,23 patients with benign breast disease and 43 patients with breast cancer with MR breast coil.The shape,border,size,number and signal intensity of axillary lymph nodes in every group were comparatively studied.The features of the axillary lymph nodes of the patients with breast cancer were compared with the histopathologic results.Results Axillary lymph nodes were detected in 15 out of the 48 healty volunteer axillas (31.25%).The shape,border,size,number and signal intensity of axillary lymph nodes had no significant difference between the patients with benign breast disease and the patients with breast cancer with no metastasis lymph nodes (LN-)and the healthy volunteers.The shape,border,size,and enhancement pattern of the patients with axillary lymph nodes metastasis(LN+)were statistically different from that of the above mentioned three groups.Conclusion MRI is an effective method to evaluate the status of axillary lymph nodes before operation.It provides useful evidences for clinical operation and treatment.

Objective To discuss clinical value of 64-slice spiral CT scanning in fast evaluation of severe multiple trauma.Methods A retrospective study was carried out on data of 72 multiple trauma patients diagnosed with systemic 64-slice spiral CT scanning in our hospital from January 2006 to June 2008.Of all,28 patients with traffic injuries,24 with falling injuries,10 with blunt injuries,6 with stab injuries and 4 with explosive injuries.Results There were 72 multiple trauma patients with mean scanning time of 10.2 seconds.The CT scanning showed both craniocerebral and thoracic injuries in 30 patients,brain injuries in 16,thoracoabdominal injuries plus pelvic injuries in 10,extremity and brain injuries in 14 and systemic soft tissue injuries in 2.Isotropic characteristics and postprocessing function of 64-slice spiral CT scanning could accurately diagnose and evaluate injury severity of multiple trauma.Conclusions 64-slice spiral CT scanning is a fast and effective method for fast evaluating injury severity of multiple trauma,for it can not only shorten checking time,reduce movements of the patients and relieve the pain of the patients,but also can avoid defects induced by respiratory movement and provide fairly integrated imaging materials by its isotropic characteristics and pestprecessing function.

Objective To investigate the relationship of single nucleotide polymorphism (SNP) on FTO gene rs9939609 with metabolism index and obesity in children. Methods One hundred and fifty-three children (age 7-11 year) were recruited in this study, 102 of them were obese and 51 of them were overweight. One hundred and sixty children with normal body weight were recruited as control. Height, weight and biochemical indicator of liver function were measured. PCR and direct sequence were applied to detect the polymorphism of rs9939609, and the frequency of the allele was calculated. Results TT or TA/AA genotype frequency on FTOgene rs9939609 was significantly different among overweight group, obesity group and normal con-trol group (χ2=23.01, P<0.001);TA/AA genotype frequency in overweight and obesity group was significant higher than that in the control group(P<0.014). The frequencies of T and A allele in overweight group, obesity group and control group was 96.25%and 3.75%, 85.29%and 14.71%, 85.78%and 14.22%respectively. There was significant difference of allelic frequency among overweight group, obesity group and control group (χ2=21.72, P<0.001). The frequency of A allele in overweight and obe-sity group was higher than that in control group (P'<0.014). Subjects with TA/AA allele had significantly higher BMI compared with subjects with TT allele. Conclusions rs9939609 of FTO gene is associated with obesity in children, and allele A on this spot may raise BMI and leads to overweight and obesity.

Objective To review our clinical experience with 41 reoperatioas of prosthetic heart valve dysfunction be-tween October 1996 and October2008. Methods There were 16 malas and 25 females with an average age of (44.5±12.6)years. All 41 patients underwent reoperation under hypothermic cardiopulmonary bypass, including 38 heart valve replace-ments, 2 disc rotations and cut of the excessive knot in 1 case. There were 13 emergency and 28 elective procedures. Results The median time for extubation was 15.3 hours and tracheostomy was needed in 5 cases. There were 6 deaths, all of which occurred in emergency cases, with a mortality rate of 15.4%. No death occurred in patients implanted with a bioprosthetic valve previously. Postoperative complications included 1 infective endocarditis, 1 intractable hiccup and 1 wound infection. Conclusion Prosthetic heart valve dysfunction is catastrophic. Early diagnosis and reoperation is mandatory.

Objective To investigate the expression of Ki-67and NF-κB in cervical lesion and their correlation with HPV infec tion.Methods 70 cases with surgically resected or cervical biopsy (70 cases cervical specimens) were selected as the research object from January 2015 to January 2017 in our hospital.The expression of Ki-67,NF-κB and PCNA were detected by immune cell chemical dyeing method,and making the correlation analysis by Spearman.Results The expression rate of Ki-67,NF-κB and PCNA in CIN and cervical invasive squamous carcinoma tissues were significantly higher than that of chronic cervicitis tissues.Spearman rank correlation analysis showed,there was a positively correlation of Ki-67 and PCNA(r=0.582,P＜0.05),there was a positively correlation of NF-κB and PCNA(r=0.603,P＜0.05),there was a positively correlation of Ki-67 and HPV infection (r=0.601,P＜ 0.05),there was a positively correlation of NF-κB and HPV infection (r=0.596,P＜0.05),here was a positively correlation of PCNA expression and HPV infection(r=0.647,P＜0.05).Conclusion There is a difference of positive expression rate of Ki-67,NFκB in different cervical lesions organizations,and Ki-67,NF-κB are associated with HPV infection,they might be involved in cervical lesions in the process,could be used as indexes for identification of cervical disease,diagnosis and evaluation.

Objective To investigate the role of a small RNA interference against VEGF in radiation sensitivity in melanoma .Methods A375 human melanoma cell lines were transplanted into nude mice ,which with malignant melanoma were randomly divided into control group , VEGF negative plasmid group and VEGF positive plasmid group, followed by 4Gy irradiation twice a week for 2 weeks.The volume of tumor was calculated twice a week, the area of tumor necrosis was assayed by HE,the expression of VEGF in tumor was determined by Western-blot and Immunohistochemical. ResuIts The expression of VEGF in VEGF positive plasmid group decreased significantly (P<0.05), VEGF positive group had more tissue necrosis, tumor growth was significantly inhibited (P<0.05).ConcIusion siRNA-VEGF in tumor injection liposome encapsulated in malignant melanoma has a role in the radiation sensitization, which provides an experimental basis for the clinical development of targeted therapy combined with radiotherapy for the treatment of VEGF gene.

Objective To analyze the occurrence of traumatic carotid cavemons fistula (TCCF) resulted from the fracture of basilaris cranii, in order to find out the related factors to outcomes and to discuss the approaches to improving prognosis.Method Data of 312 patients with the fracture of skull base complicatcd with TCCF con-firmed angiography from 1999 to 2005 were analyzed. These patients were classified into patients with disable and patients without disabed. The factors potentially impacting on outcomes were analyzed. Results The overall inci-dence of TCCF in 312 patients with fracture of basilaris cranii was 3.8% .The incideucs of TCCF occurred in pa-tients with the fracture of anterior fossa, middle fossa and posterior fossa accounted for 2.4%, 8.3 % and 1.7 %, respectively. Between two cohorts of patients, there were no difference in age, gender, number of embelization proce-dares performed and the time from injury to appearence of the first symptom except the differencc in time from ap-pearence of the first symptom to the intravascular embohzation performed (P>0.05). Conclusions A relatively high incidence of TCCF occurs in patients with middle fossa fractures, especially those with transverse or oblique fractures. Prompt diagnosis and intervention can not be emphasized in case of patients with TCCF, and non inva-sive techniques for the early detection of TCCF under certain circumstance after brain or facial trauma should be considered so as to avoid a miss in the early diagnosis of middle fossa fracture to ensure favourable outcomes.

Objective To evaluate the short-term effects of total knee arthroplasty ( TKA) , and to detect the causes and prevention of major complications. Methods It was a retrospective study since January 2010 to December 2013. There were 63 patients with 70 knees re-ceived primary TKA. KSS knee scores, HSS knee scores, and range of motion ( ROM) before the operation and during the follow-up were as-sessed and analysed. Results According to the results of follow-up and the X-ray observation, the anteroposterior radiographs showed that the artificial prosthesis were placed well and the joint gap were balanced;the lateral radiographs showed that the artificial joint prosthesis we-rer of appropriate size, good tilt angle, and good package effect. There were two cases developed peroneal nerve palsy and they gradually re-stored in 4 to 6 months after surgery. 5 cases were of poor postoperative ROM (0~60 ± 10)°. Strong analgesic medication-assisted release were conducted in 2 to 3 weeks after surgery. 1 case had patellar pain and resorted by arthroscopic lateral ligament of patellar releasing sur-gery. During the follow-up period, TKA significantly improved HSS and KSS scores compared to preoperative scores. ROM was increased from (78. 3 ± 18. 6)° preoperatively to (109. 2 ± 19. 9)° postoperative (P<0. 01). Conclusion TKA was effective to release knee pain, improve ROM and reduce the incidence of postoperative complications.

Objective: To analyze application and funding projects of National Natural Science Foundation of China (NSFC) in the discipline of burns and plastic surgery over the years, so as to provide reference for subsequent fund application and the search for new research directions in the discipline. Methods: Annual statistical reports on funding projects and Internet-based Science Information System of NSFC were used to search the application and funding situation of the science foundation projects related to the discipline of burns and plastic surgery during 1986-2018. The relevant situation was statistically analyzed during 2009-2018, including the amount of applications and grant, funding rate, and appropriation. The amount of the application and grant, funding rate, appropriation, and funding strength of multiple series (Exploration, Talent, Tools, Integration) during 2009-2018 were statistically analyzed. Main granted projects in each year during 2009-2018 were statistically analyzed. Funding situation of research direction in the discipline of burns and plastic surgery was analyzed. Results: (1) During the past 33 years, the amount of application and funding in the discipline of burns and plastic surgery were on increase trend generally. (2) During 2009-2018, the total funding rate of the science foundation was 18.63% (611/3 279), with a total appropriation of 26 167.5 ten thousand yuan. (3) During 2009-2018, the more funding projects in the discipline of burns and plastic surgery were General Program of Exploration series and Young Scientists Fund, and Fund for Less Developed Regions of Talent series in turn, their funding rates were respectively 19.11% (330/1 727), 17.72%(216/1 219), and 19.26%(47/244), with appropriations of 18 115.0, 4 450.0, and 1 780.0 ten thousand yuan and funding strength of 54.89, 20.60, and 37.87 ten thousand yuan per project respectively. In addition, the funding rate of the Key Program of Exploration series was 6.82% (3/44) with appropriation of 828.0 ten thousand yuan, and funding strength for each was 276.00 ten thousand yuan. The International (Regional) Cooperation and Exchange Programs received 15 applications, and 4 projects were funded with appropriation of 524.5 ten thousand yuan and the funding strength of 131.13 ten thousand yuan for each. National Science Fund for Distinguished Young Scholars of Talent series received 12 applications, and 1 project was funded with appropriation of 200.0 ten thousand yuan. Tools and Integration series projects received only a few or no application, and none of the application was funded. (4) The funding amount of General Programs was the most, peaking at 50 in 2012, and was decreased slightly in the past 6 years. The funding amount of Young Scientists Fund was relatively less, but it was on increase trend year by year. The funding amount of Fund for Less Developed Regions was the least relatively, with a steady increase trend in general. (5) The amount of funding projects according to the research direction in the discipline of burns and plastic surgery in the order from more to less were H1507 wound healing and scar (219, 35.84%), H1505 burns (168, 27.50%), H1508 body tissue and organs deformity, damage and repair, and regeneration (143, 23.40%), H1509 body surface tissue organ transplantation and reconstruction (53, 8.67%), H1510 cranial and maxillofacial deformities and correction (27, 4.42%), H1506 frostbite (1, 0.16%). Conclusions In recent years, the amount of science foundation projects in the discipline of burns and plastic surgery was on the rise, mainly including General Programs, Young Scientists Fund, and Fund for Less Developed Regions. In the process of science foundation application in the discipline of burns and plastic surgery, it is necessary to provide more ability training and policy support for young outstanding scientific and technological talents. At the same time, we should attach importance to international and regional academic exchanges, and provide a favorable platform for cooperative projects. Besides, research on new hotspots such as chronic wounds and prevention and treatment of scar or not in popular research such as frostbite should be strengthened.

OBJECTIVE: To discuss the clinical efficacy of adenoidectomy and tonsillectomy for the OSAHS children with sinusitis. METHOD: Reviewing 112 cases of children with OSAHS, in which the 80 patients without chronic sinusitis, 32 ones with chronic sinusitis. Among them, 103 cases of children with adenoidectomy, tonsil resection, 9 cases adenoidectomy only. We did questionnaire survey before and after surgeryand also did post-surgery clinical follow-up. RESULT: The effective rate was 90.0% for the OSAHS children without sinusitis after adenoid and tonsil ectomy surgery, while the effective rate was 46.9% for the ones with sinusitis, the efficiency was significant different in the two groups after surgery; the 16.1% children after tonsil ectomy surgery complained the incidence of pharyngeal foreign body sensation and pharyngeal lymphoid hyperplasia in children was 23.2%. CONCLUSION The efficiency was aviable for the OSAHS children without sinusitis after surgery; The efficiency was not aviable enough for the OSAHS children with sinusitis after surgery, so the latter one need adjuvant drug treatment; We also should be attention to the pharyngeal foreign body sensation and the lymphoid hyperplasia in children after tonsillectomy.
Adenoidectomy ; Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Retrospective Studies ; Sinusitis ; complications ; therapy ; Sleep Apnea, Obstructive ; complications ; surgery ; Tonsillectomy ; Treatment Outcome