Objective To explore the effect of citalopram on tau phosphorylation and memory defi-cits induced by social isolation (SI) in adult rats.Methods Sixty Sprague-Dawley adult rats (8 months) were grouped or isolation reared for six-weeks.Following the initial two-week period of rearing, citalopram ( 10 mg/kg,i.p.) was administered for 4 weeks.Novel object recognition test,Western blot and ELISA were used to detect recognitive function,the levels of tau and GSK-3βprotein,and melatonin level respectively. Results In the novel object recognition test,compared with the citalopram group(0.71±0.05) and housed group(0.73±0.13),discrimination ratio(0.48±0.15) in SI group was significant decreased (P<0.05).Tau hyperphosphorylation at Tau-1 ((0.88±0.11)),Ser396 (3.94±0.74) episodes were found and almost re-versed by citalopram at Tau-1 (1.56±0.17),Ser396 (2.31±0.24) episodes in SI group.Compared with GH group,the total level of GSK-3β(1.12±0.09) was significantly increased,while the level of Ser9-phosphoryl-ated GSK-3β(inactive form) (0.47±0.11) was significantly decreased in the SI group,both of which were reversed by citalopram (GSK-3β(0.87±0.08) and Ser9-phosphorylated GSK-3β(0.80±0.07)).The me-latonin level was decreased in SI group ((359.54±18.80)pg/ml),and citalopram could partly restore the level of melatonin (418.15±18.72)pg/ml, P<0.05).Conclusion The results demonstrate that citalopram increases the level of melatonin which negatively regulates GSK-3βand attenuates tau hyperphosphorylation and memory deficits induced by SI in adult rats.

Objective To discuss the value of pregnantal ultrasonography(US) on fetus' complicate congenital heart diseases(CHD). Methods The uhrasonographic fetures of fetus complicate CHD were analyzed and compared with the pathological diagnosis. Resells Twenty fetus' complicate CHD were diagnosed by pregnantal US. Four cases with single ventricle, two eases with transposition of great arteries,two eases with double outlet right ventricle,two cases with truncus arteriosus,three cases with hypoplastic left heart syndrome, three cases with endocardial cushion defect, two cases with hypoplastic right heart syndrome,one case with tetralogy of Fallot and one case with pulmonary atresia were included. The diagnosing according rate was 100%. Conclusions Pregnantal US can fully evaluate the fetus' complicate CHD.