Objective:To improve the accuracy of the rotary manipulations of the neck in the treatment of neck pain, according to subjective and objective clinical findings. Methods: Two groups of 60 outpatients diagnosed with neck pain were suited to the manipulation and each group was 30 cases. The diagnosis of neck pain was made with conventional clinical evaluation, including history, palpation examinations, CT scan and X-ray. Group A received a manipulation of obliquely pulling the neck. While group B received manipulation of pulling the rotated and located neck. Subjects received a maximum of 10 treatments over a 3-week treatment period. Both treatment groups were assessed with subjective (cervicodynia, neck discomfort, upper limb numbness, vertigo and tinnitus) and objective (cracking sounds). The cracking sounds during two of the manipu1ations were recorded by a microphone positioned at both sides of the neck. The sounds were compared in patients of two groups. Statistical analysis was conducted with a one-factor within-subjects analysis of variance tests and descriptive statistic. Results: Analysis of the subjective indicated that the cases with upper limb numbness were reduced from 12 to 6 in group 2, while the cases with vertigo and tinnitus were reduced from 13 to 4 in group 1. Analysis of the objective indicated two kinds of neck rotary manipulations act not only on the rotary-side facet joints, but on the opposite to the rotary side on1y in a few cases. The results showed that manipulation of pulling the rotated and located neck has a small action range and centralized action points, while manipulation of obliquely pulling the neck has many action segments and discentra1ized action points. Conclusion: It was concluded that the rotary manipu1ations act mainly on the rotary side, not on the opposite, and that manipulation of obliquely pulling the neck has a wider action range and a better therapeutic effect on cervical upper-segment lesions, while manipulation of pulling the rotated and located neck has a limited action range and is suitable for cervical lower-segment lesions. There were some relations between the joint sounds during the manipulation and clinical curative effect.

Objective To compare the efficacy in patients with severe pancreatitis treated by minimally invasive surgery and open surgery. Methods Seventy-three cases of patients with severe pancreatitis which were treated in our hospital between June 2011 and June 2015 were divided into group A (n = 37) and group B(n =36). Group A were treated by minimally invasive surgery while group B by open surgery. The perioperative stress reactions , postoperative inflammatory reactions , incidence of complications and mortality were compared between the two groups. Results The proportion of patients with perioperative (0+1) level pain in group A (70.3%) was significantly higher than that in group B (44.4%) (P < 0.05). Seventy-two hours after surgery, the CRP, TNF-α and IL-6 in group A were lower than those before surgery and those in group B while APN in group A was higher than that before surgery and that in group B (P < 0.05). The incidence of pulmonary infection and mortality in group A (27.0%, 5.4%) were lower than those in group B (50.0%, 22.2%) (P < 0.05). Conclusions Minimally invasive surgery in the treatment of severe pancreatitis has slight trauma. The postoperative pain is mild and complications are fewer , and the mortality is low.

In terms of the characteristic of exonuclease I that can selectively catalyze the degradation of single-stranded DNA, we report a new sensitive electrochemical biosensor for the detection of breast cancer cells by using the aptamers of MUC1 that is overexpressed on the surface of MCF-7 cells. Because the binding of aptamer and MUC1 that is over-expressed on the surface of breast cancer cell MCF-7 can prevent its hybridization with the complementary probe DNA, the unhybridized single-stranded probe DNA that is immobilized on the electrode surface will be selectively digested by Exonuclease I and result in the removal of the signal molecule methylene blue. Therefore, by tracing the changes in electrochemical responses, the biosensor can be used to sensitively detect MCF-7 cells in a linear range from 103 to 106 cell/mL with a detection limit of 330 cell/mL, and easily distinguish the control islet β cells with high specificity.

Objective To investigate the clinical distribution of carbapenem-resistant Enterobacteriaceae(CRE)strains separated in this hospital and the situation of its production of carbapenem enzyme.Methods The production of carbapenem enzyme by CRE strains was confirmed by using modified Hodge test,the situation of the production of metallo-beta-lactamases by CRE strains was screened by using imipenem-EDTA double-disk synergy test,and the clinical distribution of CRE strains was retrospectively ana-lysed.Results 37 strains of CRE isolated in this laboratory were screened by using instrument method and verified by using disk diffusion (K-B)method.It showed an increasing trend from 2012 to 2014 in the amount of CRE strains.In terms of bacterial spe-cies,K.pneumonia(1 6 strains)was the main kind of carbopenems-resistant strains,followed by E.coli(6 strains),Ser.marcescens(6 strains)and E.cloacae(4 strains).CRE strains were mainly isolated from geriatric ward and intensive care unit(ICU).Sputum,u-rine and blood specimen were key sources of CRE strains.Modified Hodge test confirmed that 36 strains of CRE were the strains that can produce carbapenemase,including 4 strains of K.pneumonia,3 strains of E.cloacae,and 1 strain of E.asburiae,and strains producing metallo-beta-lactamases were confirmed by using imipenem-EDTA double-disk synergy test.Conclusion Elderly patients with underlying diseases are susceptible population of CRE hospital infection and are primary preventive targets.The principal mechanism of carbapenem-resistant CRE strains in this hospital is the production of carbapenemase and production of metallo-β-lac-tamases in a small number of strains.

Objective To discuss the value of combining the physiological and operative severity score for enumeration of mortality and morbidity(POSSUM)and acute physiology and chronic health evaluation Ⅱ(APACHE Ⅱ)in severe acute pancreatitis(sAP).Methods 60 cases of SAP were divided into surgery group(n=15)and non-surgical group(n=45),Non-surgical group was vahde with APACHE Ⅱ score,the surgery group with POSSUM score,decided to continue conservative treatment or surgery treatment,Observation of two ways with the complications and death.Results The rates of compHcafions and death were(0.37±0.08)and(0.27±0.09)in Single-operation group(n=15)higher than(0.76±0.14)and(0.61±0.15)in surgical group(n=11)(t1=3.125,t2=3.211,P＜0.01);APACHEⅡ score of mortality in operation group and non-surgical group were no significant difference(x2=2.28,x2=1.98,P＞0.05);APACHE Ⅱ score were(10.12±6.27)in survival group(n=46)were lower than (25.75±7.90)in death group(n=14)(t=2.525,P＜0.05.Conclusion The score of dynamic APACHE Ⅱ has better effect to judge the timing of surgery,and the score of POSSUM has high value to predict post-operative complications and deaths occurred in patients with severe acute pancreatitis.

ObjectiveTo investigate the biological significance and mechanism of VEGF-C in esophageal tumor development,and correlation of CNTN-1 level with VEGF-C.MethodsThe expression of VEGF-C and its receptors in esophageal squamous cancer cell (ESCC) and in corresponding noncancerous esophageal tissue specimens were detected by real-tithe PCR.Esophageal squamous cancer cell line TE-1 was transinfected by VEGF-C overexpression and gene silencing vectors,respectively,and the relative amount of C/EBP bound to CNTN-1 promoter was determined by quantitative ChIP,to explore the possibility that VEGF-C was involved in development of esophageal cancer through mediating transcription of CNTN-1.ResultsThe mRNA levels of VEGF-C was significantly higher in ESCC than in normal esophageal tissues.VEGF-C expression was significantly increased in VEGF-C-overexpressing TE-1 cells compared to untransfected cells (mock).Cells transfected with either of the VEGF-C targeting shRNA vectors,shRNA-1 and shRNA-2,showed reduced VEGF-C transcripts (P ＜ 0.01 ).Expression levels of VEGF-C and CNTN-1 mRNA correlated significantly with each other.The binding site of C/EBP in CNTN-1 was detected by ChIP,and the relative amount of C/EBP binding to CNTN-1 promoter was significantly increased in TE-1 after transfecting by VEGF-C overexpression vector ( P ＜ 0.05).ConclusionVEGF-C and its receptor are highly expressed in esophageal cancer tissues,which may be associated with ESCC carcinogenesis and development.VEGF-C may influence on growth and migration in TE-1 cells through CNTN-1.

BACKGROUND: Although hand urgical doctors have drawn the attentions to carpal diseases mainly manifesting as carpalinstability, it is still expected to carry on the researches on biomechanical properties of Chinese carpal ligaments. OBJECTIVE: To understand the biomechanical properties of partial carpal ligaments so as to provide experimental evidence for the development ofclinical hand surgery. DESIGN: A simple specimen study.SETTING:Department of Orthopaedics,Kunming General Hospital of Chengdu Military Area Command of Chinese PLA,and Institute of Clinical Anatomy, First Military Medical University of Chinese PLA.PARTICIPANTS: Sixteen fresh specimens from Chinese adult wrists were obtained from the Departmentof Anatomy, First MilitaryMedical University of Chinese PLA and the experiment was performed in the Laboratory of Biomechanics. INTERVENTIONS:The partial carpalligaments were stretched till fragmentation of ligaments by using the SWD-10materialtesting machine, at the rated velocity of 5 mm per minute so as to determine the biomechanical properties of carpal ligaments. MAIN OUTCOME MEASURES:The tolerated maximum tension and rigidity during fragmentation of radioscaphoid ligament,radioscaphocapitate (RSC) ligament, radiolunate ligament, ulnolunate (UL) ligament, ulnotriquetral(UT) ligament,ulnar bursa,scapholunate interosseous(SLI) ligament,and lunotriquetral interosseous(LTI) ligament. RESULTS: Among the ligaments attached between distal radioulnar bone and carpal bone, the maximum tension and rigidity of UL ligament were the strongest, (219.2 ±55.4) N and (65.5 ± 19.6) N/mm2, of UT ligament and ulnar bursa were the weakest, (54.0 ±25.5) N, (17.8 ± 6.0) N/mm2and (58.7 ± 17.6) N, (13.4 ±4.7) N/mm2 respectively, of SLI ligament was stronger, (286. 1 ±90.8) N, (95.5 ±40.0) N/mm2, and of LTI ligament were more stronger, (375.3 ± 52.6)N, (179.0 ± 39.0 N/mm2.CONCLUSION: Among carpal ligaments, the maximum tension and rigidity of proximal row of intercarpal ligaments were stronger than those of the ligaments attached between distal radioulnar bone and carpal bone. Of proximal row of intercarpal ligaments,the maximum tension and rigidity of LTI ligament were stronger than those of SLI ligament. Of the ligaments attached between distal radioulnar bone and carpal bone,the maximum tension and rigidity of UL ligament were the strongest.

Objective To study the clinical significance and changes of serum TNF-α、IL-6、IL-8、PAFand AGT-Ⅱ in patients with severe acute pancreatitis(SAP). Methods Serum TNF-α、IL-6、IL-8、PAF、AGT-Ⅱ values of all patients were measured by ELISA and radioimmunoassay. 30 cases with mild AP(MAP group),50 cases with SAP(SAP group) and 30 non-pancreatitis patients as control group. Results The level of TNF-α, IL-6, IL-8, PAF, AGT-Ⅱ of acute phase and recovery phase in SAP group and the MAP group were significantly higher than the normal control group(all P < 0.01); the level of TNF-α、IL-6、IL-8、PAF and AGT-Ⅱ of acute phase in SAP group were significantly higher than the MAP group(all P <0.05); the level of TNF-α、IL-6、IL-8、PAF and AGT-Ⅱ of re-covery phase in SAP group were significantly lower than the acute phase(all P <0.05);the level of TNF-α、IL-6、IL-8、PAF and AGT-Ⅱ of died in SAP group were significantly higher than the survivors (all P < 0.05～0.01). Conclu-sion The level of TNF-α、IL-6、IL-8 with the severity of SAP's condition and prognosis is closely related, The level of AGT-Ⅱ in SAP group were significantly higher than the MAP group.

Objective To study the significance and expression relationship among STAT1,STAT2 and hMLH1 ,hMSH2 proteins in hepatocellur carcinoma(HCC). Methods SABC immunohistochemistry method was used to detect the expression of STAT1,STAT2,hMLH1 and hMSH2 proteins in cancer tissues and paracancer tissue from 37 patients of HCC. Results The positive rates and expressive levels of STAT1,STAT2,and hMLH1, hMSH2 in HCC was significantly lower than those in paracancer liver tissues(P

The influence of Rosa davurica pall juice on pulmonary adenoma induced by urethan was observed in the experiment, which lasted for 20 weeks. 250 mice were divided into 4 groups at random. Group A was set as control receiving basal diet and tap water only. Another 3 groups were given basal diet, however, group B was given Rosa davurica pall juice 0.2ml/20g boody weight once a week, group C 0.1% urethan solution for drinking freely and group D 0.1% urethan solution plus the juice. The mouss body weight was weighed once a week; the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), the level of lipoperoxides (LPO), lymphocyte transformation and hemolysin were determined once a month, the pulmonary pathological examination was made also once a month. The obtained data were carried out with a 2?2 factorial analysis. The result were as follows:(1)the incidence of pulmonary adenoma of group C(100%) was significantly higher than that of group D (69%) at the e-nd of experiment (P