Objective:To study the value of clinical application of 16-slice spiral CT in the diagnosis of acute chest pain.Methods:53 cases who presented with acute chest pain were included in the study.All of the patients underwent CTA examination to evaluate the coronary arteries,pulmonary arteries,thoracic and abdomen aorta.Coronary arteries angiography and interventional therapy was performed in 6 of the 53 cases.Multi-planar reconstruction(MPR),maximum intensity projection(MIP),curved-planar reconstruction(CPR)and volume rendering(VR)were used to display coronary arteries,pulmonary arteries,thoracic and abdomen aorta.The image quality was then evaluated according to whether the examinations can fulfill the clinical demand for the differential diagnosis of acute chest pain.Results:In the 53 cases,there were 33 cases with coronary arteries disease,9 cases with aortic dissection or dissecting aneurysm,2 cases with pulmonary arteries embolism,2 cases with spontaneous rupture of esophagus,2 cases with pneumothorax.Conclusion:CTA examination with 16-slice spiral CT can display the coronary arteries,pulmonary arteries,thoracic aorta in 11～13s with good image quality.It is of great value of clinical application in the etiological diagnosis for the patients with acute chest pain.

Objective To explore CT angiography(CTA) method for the influence of the cerebral angiography ,looking for dif-ferent situations using which kind of scan mode can achieve the best results .Methods 350 CTA patients were selected and tested by 4 kinds of scanning methods which were subtraction scan (236) ,experience value scan (13) ,smart tracking scan (68) ,smart tracking and subtraction combined(33) .To compare and analyze that four kinds of scanning methods ,to explore which kind of method under what circumstances can get the best examination results .Results Smart tracking and subtraction combined with scan method and subtraction scan method for excellent rate of image quality were 93 .93% and 93 .64% respectively ,there were no sig-nificant statistical differences between them .Experience value method and smart tracking method for excellent rate of image quality were only 76 .92% and 66 .18% ,there was significant difference in statistics .Conclusion Subtraction scan can be used as a conventional scan method .In low dose test bolus of time-density curve detection failure cases ,smart tracking and subtraction combined with scan method was used to get the image quality with subtraction scan method ,it is an effective complement of subtraction scan method ,is worth generalization .

The construction of on campus practice base is crucial for improving the quality of practical teaching.We put forward a construction pattern of‘grid type' pharmacy teaching comprehensive laboratory.It covers three-leveled experimental platform and professional teaching laboratory according to the research and development process of new drug.It also forms the communication network between learning and working demand of students.This provides new ideas for the construction of pharmacy teaching laboratory in newly-built university.

Objective To investigate the method and the effect of posterior approach low lumbar spinal nerve root sheath decompression under microendoscope (MED-Ⅱ). Methods Thirty-nine patients with low lumbar disc herniation, accompanying severe preoperative limb numbness and radiculalgia, who were found obvious edema of nerve root with adhesion during surgery, entered the study. In the course of lumbar discectomy, a self-made mini-scalpel was used for the incision and decompression of the nerve root sheath, in which a 3～5 mm longitudinal incision was made along the dorsal side of the nerve root. Results Decompression was achieved under microendoscope (MED-Ⅱ) in all the 39 patients. Limb numbness and radiculalgia subsided completely in 17 patients within 24 postoperative hours, while the symptoms trailed off within 2 weeks following the surgery in the remaining patients. The time to complete disappearance of the symptoms was 1～14 d (mean, 6 5 d). Conclusions Minimally invasive spinal nerve root sheath incision and decompression can give a prompt relief from limb numbness and radiculalgia, improving the recovery of nervous system. Lumbar spinal nerve root sheath incision and decompression under microendoscope (MED-Ⅱ) is a safe and effective procedure.

Objective To explore the technical feasibility and effects of ant erior cervical discectomy and interbody fusion using endoscopic technique. Metho ds A retrospective study was undertaken in 23 patients with single segmental cer vical fracture and dislocation or cervical spondylotic myelopathy. There were 14 males and 9 females with an average age of 41.5 years (range, 23 to 64). The fr acture and dislocation patients consisted of 2 cases with complete tetraplegia e ither of C3,4 or C4,5, 3 with incomplete tetraplegia of C5,6 and 2 of C4,5 free from nervous symptoms. The operative procedures included anterior cervical disce ctomy and interbody fusion with insertion of cage or/and plate fixation through an endoscopic working canal. Results All 23 cases were followed-up from 6 to 18 months. The operative incisions healed smoothly. All patients achieved interbody fusion, and no loosening or displacement of the cage and plate occurred. For th e cervical fracture and dislocation patients, by Frankel's classification, 2 cas es with complete tetraplegia showed no any improvement, 2 cases with incomplete tetraplegia improved from grade C preoperatively to grade D postoperatively, 1 c ase with incomplete tetraplegia upgrade from C to E, 2 cases without nervous sym ptoms maintained preoperative status. For cervical spondylotic myelopathy patien ts, according to Odom's scoring system, 10 cases were assessed as excellent (dis appearance of all symptoms), 5 good (alleviation of main symptoms), 1 fair (alle viation of partial symptoms). 94% patients gained excellent and good results. No complications occurred in this series. Conclusion With the improvement of the t echnique and instrument, the endoscopic procedures can be used for anterior cerv ical discectomy, interbody fusion and internal fixation. It presents microsurgic al advantages such as minimal incision, less traumatic reaction, clear intraoper ative visualization, and less postoperative discomfort comparing to traditional approach.

ObjectiveTo explore the role of CXCL16/CXCR6 axis on the metastasis of human lung cancer.MethodsImmunohistochemistry and immunocytochemistry analysis were performed to detect the expression of CXCL16/CXCR6 in human lung cancer samples as well as A549,95D and H292 cell lines,respectively.The effects of CXCL16 on the viability and invasiveness of the three lung cancer cell lines were examined by MTT and in vitro invasion assay,respectively.ResultsHuman native lung cancer cells co-expressed CXCR6 and CXCL16 protein.Compared to the normal lung tissues,there was a stronger specific staining for both CXC16 and CXCR6 protein in the lung cancer tissues.Three kinds of lung cancer cell lines,including A549,95D and H292,all expressed CXCL16 and CXCR6 protein.Furthermore,human recombinant CXCL16 significantly promoted the viability and invasiveness of A549,95D and H292 cells.The stimulated action of CXCL16 on lung cancer cell lines could be effectively blocked by CXCL16 neutralizing antibody.Conclusion CXCL16 and CXCR6 protein are co-expressed in human native lung cancer.CXCL16 is able to promote the viability and invasiveness of lung cancer cell lines,which might be the molecular mechanisms on the metastasis of lung cancer.

Objective To compare the biomechanical stability of the multiaxial inter-vertebral and vertebral pedicle screw fixation in vertebral fracture fixation of the spinal fracture model. Methods Six lumbar fracture models were made on fresh calf lumbar spine specimens at L1 -L5 to compare the stability of four transpedicular multiaxial screws and six transpedicular multiaxial screws by examining the range of motion (ROM) in flexion, extension, lateral bending and torsion. Results Biomechanical experiment found that four multiaxial screws transpedicular fixation specimen exhibited a significantly larger ROM in flexion and extension than the intact specimens; while six multiaxial transpedicular screw fixation specimen exhibited a smaller ROM than the intact specimens (t =4. 844,P ＜0. 01 ;t =3. 722,P ＜0.05 ). The ROM of six multiaxial transpedicular screw fixation specimen was significantly smaller than that of four multiaxial transpedicular screw fixation specimen in flexion, extension, lateral bending and rotation ( P ＜ 0. 01 ). Conclusions Compared with four multiaxial transpedicular screw fixation, six multiaxial transpedicular screw fixation exhibits a significantly larger stability in flexion, extension, lateral bending and rotation, as provides theoretical basis for treatment of thoracolumbar fractures with pedicular screw fixation.

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Objective To investigate if the quantum resonance spectrometer(QRS) can detect the schizophrenic symptoms in clinical applications.Methods According to the result of QRS test and ICD-10 diagnostic criteria and exclusion criteria, subjects were divided into patient( n = 65 ) and control groups( n = 100).11 schizophrenia-related symptoms were detected by QRS.Data was analyzed using SPSS13.0 statistical software statistical significance was set at P＜0.05.Results Sensitivity and specificity of QRS in the schizophrenic symptoms detection respective were hallucinations (0.887,0.851 ), lenovo undisciplined (0.847,0.868 ), thought insertion (0.800,0.843 ), logical thinking perversion ( 0.813,0.879 ), delusions ( 0.873,0.884 ), apathy ( 0.603,0.891 ), will decrease (0.883,0.886), environmental disorientation ( 0.574,0.837 ), self-disorientation ( 0.609,0.802) , double disorientation (0.603,0.843 ) and insight (0.862,0.869).Conclusion QRS detection possess higher authenticity, consistency and the high diagnostic value in the schizophrenic symptoms, special in hallucinations,thought disorder,insight symptoms.

Objective To investigate effect of a new lumbar dynamic internal fixation system(DIFS)on the stability and intradiscal pressure of lumbar segment.Methods Eight fresh calf lumbar specimens were collected to build test models,which were loaded with pure moments of 8 N·m in flexion/extension,lateral bending,and rotation planes.The test states included intactness,destabilization,dynamic fixation,semi-rigid fixation,and solid fixation.The data were collected from the third loading cycle to determine range of motion and neutral zone of corresponding segments,and the intradiscal pressure.Results Destabilization significantly increased segmental range of motion and neutral zone in three motion planes without obvious influence on intradiscal pressure.The three fixation systems were all able to stabilize and load destabilizing segments.DIFS restored the range of motion of unstable segment to 77％,60％,61％ and 70％ of the intact level in anterior flexion,posterior extension,lateral bending and rotation planes.Meanwhile,DIFS bore 45％,29％ and 40％ of tbe intradiscal load in lateral bending,rotation and flexion directions,but 100％ of the intradiscal load in back extension.The stability and intradiscal pressure of adjacent segments were not affected by the test states.Conclusion DIFS can effectively restore range of motion of unstable segments and share appropriate intradiscal load in most directions.