Objective To investigate the change of S-100B in serum in rats at varions times after exposure to different whole-brain irradiation doses and to explore the characteristics of the S-100B change in early radiation-induced brain injuries. Methods A model of brain radlation-induced injuries was estabhshed in the rat after whole-brain irradiation with a single dose of 2, 10, or 30 Gy of 4 MeV electron. The concentration of S-100B in serum was detected by means of enzyme-linked immunoserbent assay (ELISA) at different time points (1,6,12,24,3 days, 1 week, 1 month) after exposure. Results In terms of measuring time points, the serum concentration in all groups reached the fwst peak 6 hours after irradiation, declined after 12 hours, rose again after 24 hours, and started to decline again until the last time point (1 month), with the exception of the 2 Gy group, which reached the second peak at 3 days after irradiation. Conclusions The S-100B serum concentration was changed in the rats after whale-brain irradiation and these changes are positively related to the irradiation amount. It seems that the higher the dose, the higher the serum concentration and the differences between the dose groups are significant (via multiple regression models). The S-100B may be a valuable serum marker for the brain irradiation injury.

The marrow stromal cells of adult New Zealand rabbits cultivated and induced in vitro were used to form MCCAB by mixing,seeding and solidifying methods with the aid of alginate. The MCCABs were auto-transplanted intramuscularly into the rabbits for 4 to 8 weeks. The alginate-cancellous bone matrix composites or the cancellous bone matrix alone were implanted as control. The effectiveness of bone formation was assessed by means of roentgenography and histology.The results showed that the osteogeneses of MCCABs were better than those of the alginate-cancellous bone matrix composites and of the cancellous bone matrix. In the MCCABs, both intramembranous and cartilaginous osteogenesis were seen with the former predominating. In the control, only slight cartilaginous osteogenesis was seen. The results suggested that the osteogenesis of the MCCABs constructed by using tissue engineering method was obvious when transplanted intramuscularly, therefore, this kind of tissue-engineered bone could be an effective way for clinical application.

To investigate the feasibility of using coral and other materials as scaffolds for bone tissue engineering, coral, coral hydroxyapatite(CHA), cancellous bone matrix and other natural biomaterials served as culture scaffolds of osteoblasts were manufactured. The results showed, in addition to PLA, PGA, PLGA and other synthetic polymers, some natural biomaterials are also ideal scaffolds materials for bone tissue engineering.

Objective To investigate the clinicopathological features of central nervous system hemangioblastoma in von Hippel-Lindau syndrome.Methods The data of 10 patients with central nervous system hemangioblastoma in our hospital since 2013 were analyzed retro-spectively,and the clinicopathological features of central nervous system hemangioblastoma in von Hippel-Lindau syndrome were summarized. Results The macroexamination result showed that most tumor lesions were found in the cerebellum and medulla oblongata,with cystic chan-ges,size from 1 cm to 5 cm,the average size was (3.1 ±0.2)cm,clear boundary,intracapsular yellow cyst fluid.The microscopy result showed tumor foci with a rich blood supply,endothelial cell proliferation and hypertrophy in vascular,which arranged in nests or lobulated mesenchymal cells,the cytoplasm of stromal cells was abundant and lightly stained,a rich lipid was seen,with vesicular or foam.Conclusion The von Hippel-Lindau syndrome is usually cystic lesion,the microscopic examination shows tumor foci with rich blood supply,endothelial cell proliferation,hypertrophy and other changes in vascular.

Purpose: To study the treatment of metastasis of late digestive tract cancer?Methods:58 patients with late digestive tract cancer were divided into two groups. One group (reference group) of patients 28 cases were underwent chemotherapeutic treatment while another (treatment group) 30 cases were treated with DDP by CHPP. Results:Through DDP by CHPP (treatment group) the treatment show effectiveness as much 63.3% higher than that of reference group as 39.3%. This indicates the fact that there were significant differences between the groups ( P 0.05)Conclusions: DDP with Cisplatin by CHPP on patients with late digestive tract cancer, especially those patients followed by ascites, can evidently enhance the effectiveness of the treatment.

Objective: To observe the expression of urokinase-type plasminogen activator receptor (uPAR) in human tongue squamous cell carcinoma Tca 8113 cells at different dosages of heat shock. Methods:The expression of uPAR protein in Tca 8113 cells was examined using immunohistochemical technique (IH) and flow cytometry (FCM) after the cells had been treated at 37,40,43 or 45 ℃ for 40 min. Results:The fluorescent intensity of uPAR protein of Tca 8113 cells treated at 37,40,43 and 45 ℃ was 11.36?0.06,9.98?0.12, 10.37?0.05 and 11.31?0.10 respectively.Conclusion:Heat shock can reduce the expression of the uPAR protein of Tca 8113 cells.

Objective To construct the wt-p53's eukaryotic expression vector pE6/p53/GFP that was controlled by the radiation induced promoter and research its functions.Methods Radiation response element E6 was synthesized by gene synthesis.The wt-p53 cDNA sequence was prepared from pcDNA3.1(+)/p53 plasmid by PCR.IRES2-EGFP report gene segment was prepared from double cistron expression vector IRES2-EGFP by enzyme digestion.After sequenced and identified,the recombinant plasmid was transformed into H1299(p53-/-)cell with Lipofectamine 2000,and the cell lines in stable expression was screened by G418.In the H1299(p53-/-)cell transfected with the recombinant plasmid or without,wt-p53 mRNA expression was analyzed by RT-PCR,the p53 expression by Western blot when exposed to 4 Gy 8 MV X ray for 0,3,8,12,24,36 h or when exposed to 0,1,2,4,8 Gy 8 MV X ray for 12 h.The cell cycle of H1299(p53-/-)cell transfected stably with the recombinant vector was analyzed by flow cytometry after exposed to 4 Gy 8 MV X ray.Results The recombinant pE6-p53/GFP plasmid had been constructed correctly and the expression of p53 gene in the transfected H1299 cell lines had been determined.After 4 Gy X ray radiation,the expression of wt-p53 protein had a significant rise.The transfected H1299 cell lines stopped in G1 stage after radiation and their cloning efficiency decreased notably.Conclusion We had constructed successfully the recombinant pE6/p53/GFP plasmid that was regulated by radiation induced response element E6.This provides experimental data for radiation-gene therapy of non-small lung cancer.

0.05),but it in thin nasal feeding tube group was significantly lower than that in the other two groups(P

Objective To study the adjustment principle and optimizing methods of the image processing parameters of Computed Radiography(CR), including the image density, contrast degree, tissue equalization, spatial frequency, LUT curve,etc. Methods Konica REGIUS MODEL170 CR was applied to adjust various image processing parameters on its CS-1 console. Results Images of all parts of body could be optimized and improved. Conclusion High-quality medical images are available only if the adjustment methods of image processing parameters are completely comprehended and reasonably applied.

ObjectiveTo study the self-choice right of the patients who visit psychology department in TCM hospitals and provide a reference for further TCM psychological treatment application.Methods Self-choice and visiting condition inquiry enacted by ourselves was used in the survey.It is an unregistered and on-sight investigation.The inquiries were distributed by doctor.Patients were instructed by their doctors in the process.Results64.35％ of patients in psychology department of TCM hospitals select treatment methods by themselves.TCM is the first choice among 66.96％ of patients in psychology department of TCM hospitals.44.35％ of patients visiting psychology department refuse western medicine due to their side effects.6.09％ of patients in psychology department of TCM hospital know a little about psychological treatment; however,some of them believe in psychological treatment.ConclusionThe research finds out the main methods of self-choice right among patients visiting psychology department of TCM hospital.It proposes that self-choice right should be respected,at the same time,doctors are responsible to guide patients to understand the etiology of diseases.These efforts will contribute to harmony relationship between doctors and patients and facilitate their recovery and rehabilitation.