Objective To investigation the relationship between health management and prognosis of chronic prostatitis. Methods Three hundied and forty-six cases of chronic prostatitis were divided into two groups. The control group treatment was local washing and medicine for three months. The experiment group treatment was local washing, medicine, and health management for three months. NIH-CPSI was used for the results. Results There was no significant different between the experimental group(165 cases)and the control group(154 cases)before treatment in NIH-CPSI. The effective rate of the the experimental group was 88.5%, and that of the control group was 64.9% after treatment. It is effective for cure and the prognosis of the experiment was better than the control (P<0.05). Conclusion It is suggested that it is more effective for the prognosis of chronic prostatitis after the intervention of health management.

Objective To introduce the wavelet zero -cross stimulation algorithm and based on which to design and implement the cochlear implants. Methods Through DSP and ICETEK-VC5416, the WZS speech coding strategy was simulated, and also the presented WZS strategy was compared to CIS strategy by hardware implementation and computer simulation. Results The processed audio signals, output from eight channels, were synthesized and played through loudhailer and could be heard with high fidelity. Conclusion The wavelet zero-cross stimulation(WZS) strategy, which generates stimulating pulsatile series at zero-cross points in the domain of wavelet transform, can keep the temporal correlation and coherence of speech remained in stimulating pulsatile series and reflects the characteristic of temporal coding for cochlea.

An inflatable liquid epidural balloon was used to simulate an intracranial space-occupying lesion.Two groups of dogs with 8 in each were employed.Under different conditions and time intervals,intracranial pressure(ICP)was continuously recorded,and pressure-volume index(PVI),volume-pressure response(VPR),and cerebrospinal fluid pulse pressure(CSFPP)were measured silmutaneously.It was found that PVI was in significant negative linear correlation with ICP,while VPR and CSFPP in significant positive linear correlation with IVP(P

Objective:To study the effects of LeFort I osteotomy in the treatment of old maxillary fracture.Methods:30 patients with old maxillary fracture were treated with LeFort I osteotomy,the rehabilitation of occluding relation and masticatory function were observed after operation.Results:28 patients had well-pleasing occluding relation and masticatory function; 2 patients dissatisfied with their masticatory function, because of unbalance of nerve-muscle after their occlusal plane were rebuilt.Conclusion:LeFort I osteotomy can be a method in the treatment of old maxillary fracture.

Purpose To investigate the correlation of the expression of Cyclin D1 and BRAF ( V600E) mutation in papillary thyroid carcinoma ( PTC) and their clinical significance, and to analyze their role in the pathogenesis of PTC. Methods The expression of CK34βE12 and MC protein was detected by immunohistochemical EnVision method in 52 cases of PTC and 52 cases of thyroid benign lesions (25 cases of nodular goiters, 15 cases of Hashimoto’s thyroiditis, 12 cases of follicular adenoma), and the mutation status of BRAF gene protein in above specimens were detected by polymerase chain reaction and DNA sequencing. Expression of Cyclin D1 and BRAF (V600E) mutation in papillary thyroid carcinoma and their correlation clinicopathologic features were also analyzed. Results The positive rate of Cyclin D1 in 88 cases of PTC was 84. 6%,and the immunoreactivity score (4. 6 ± 2. 4), compared with thyroid be-nign lesions (1. 3 ± 1. 6), was statistically significant difference (t=8. 525, P<0. 01). Expression of Cyclin D1 was closely related with lymph node metastasis, capsular invasion, tumor stage Ⅲ+Ⅳ and BRAF (V600E) mutation (P<0. 05), but not related with age, gender, number and diameter of tumor nodules. In 52 cases of PTC BRAF gene mutation rate was 63. 5%, but in thyroid benign lesions mutation rate was 0, with significant difference (P<0. 01). The BRAF mutation is closely related with lymph node metastasis, capsular invasion and tumor stage Ⅲ+Ⅳ(P<0. 05), but not related with age, gender, number and diameter of tumor nodules. The expression of Cyclin D1 in BRAF (V600E) mutation group was stronger, the positive expression rate was significantly higher than that of wild group (100% vs 57. 9%), and expression of the mean score was higher than that of the wild group (5. 7 ± 1. 6 vs 4. 0 ± 2. 5);the comparison between the two groups showed statistical difference (t=2. 652, P<0. 05). Conclusions The expression of Cyclin D1 and BRAF (V600E) mutation are positively related, which are also closely related with the occurrence and development of PTC, and can be used as an index to predict the invasion and metastasis in PTC.

laparoscopic technology has been widely used in the fields of surgery such as general surgery etc. It has several advantages,such as directly showing abdominal organs,demonstrating operation procedure and being easy to make and spread operation video material. Laparoscopic system should be used for intern teaching in general surgery department to improve their learning and performance.

The CD19-targeted chimeric antigen receptor (CAR) T-cell treatment has achieved clinical success in treating B-cell lineage hematopoietic malignancies. This accomplishment involved the precise and efficient elimination of tumor cells by tumor-associated an-tigen-redirected immune cells. As a result, the reinitiation of several CAR T-cell (CART) based clinical trials in solid tumors was promot-ed. However, developing a feasible and efficient CAR T based therapeutic modality for solid tumors is urgently needed given the differ-ential properties between liquid and solid tumors. In this review, we discuss the advances in the management of cytotherapeutic mo-dality for solid tumors. The aspects considered include toxicity management, target selection, and primer or conditioning treatment.

Objective To compare the clinical efficacy between laparoscopy and open cholecystectomy for patients of acute calculous cholecystitis. Methods Fifty patients of acute cholecystitis from February 2014 to February 2017 were divided into control group treated with open cholecystectomy and observation group with laparoscopic cholecystectomy;a retrospective analysis was conducted to compare time of resuming peristaltic sound ,postoperative 24h VAS score and hospitalization time. Results There were shorter time of resuming peristaltic sound,higher postoperative 24 h VAS score and shorter hospitalization time in observation group when compared with those in control group (P < 0.05). Conclusions For patients of acute calculous cholecystitis , laparoscopic cholecystectomy,is conducive to shortening patient′s recovery time,alleviating patients′ pain signifi-cantly and decreasing the incidence of complication.

The construction of on campus practice base is crucial for improving the quality of practical teaching.We put forward a construction pattern of‘grid type' pharmacy teaching comprehensive laboratory.It covers three-leveled experimental platform and professional teaching laboratory according to the research and development process of new drug.It also forms the communication network between learning and working demand of students.This provides new ideas for the construction of pharmacy teaching laboratory in newly-built university.

BACKGROUND: Previous research has indicated that both insulin-like growth factor Ⅰ (IGF-Ⅰ) and basic fibroblast growth factor (bFGF) play an important role in cell proliferation and differentiation. However, the effect on biological characteristics of human dental papilla mesenchymal cells (hDPMCs) still remains unclear. OBJECTIVE: To research the effect of IGF-Ⅰ and bFGF on the proliferation and differentiation of hDPMCs.METHODS: hDPMCs were isolated and cultured in DMEM/F12 culture media containing 1% or 10% fetal bovine serum. The fourth-passaged hDPMCs were incubated in culture media containing 0, 0.1, 1, 10 and 100 μg/L bFGF and 0, 25, 50, 75 and 100 μg/L IGF-Ⅰ(0 μg/L as control group), respectively. At 96 hours after culture, proliferative activity was measured with MTT assay. The corresponding growth factor culture media were used in 10 μg/L bFGF group, 100 μg/L IGF-Ⅰgroup, bFGF + IGF-Ⅰ group, and control group, respectively. At days 1, 3, 5, and 7 after culture, the proliferative activity was detected using MTT assay, and alkaline phosphatase (ALP) activity was measured using modified enzyme kinetics method. RESULTS AND CONCLUSION: At the 0-100 μg/L mass concentration scope, both bFGF and IGF-Ⅰcould accelerate proliferation of hDPMCs, and the proliferation ability of bFGF was superior to that of IGF-Ⅰ; moreover, the combination of bFGF and IGF-Ⅰcaused a synergetic action to proliferation of hDPMCs. The maximal valid concentration of bFGF was 10 μg/L, and the maximal action concentration of IGF-Ⅰwas 100 μg/L. At 0-7 days, the effect of bFGF on the ALP activity of hDPMCs was not obvious, but the effect of IGF-Ⅰon ALP activity of hDPMCs became greater with the time passing; furthermore, the combination of bFGF and IGF-Ⅰcould generate a synergetic action on increasing the ALP activity.