Objective To introduce an interpersonal relationship regulating technique in the static state of Qigong to enrich modern clinical psychotherapies. Methods Based on previous therapeutic experience, we introduce one specific technique of the Systematic Psychotherapy of TCM (SPT): Interpersonal relationship regulating technique in the static state of Qigong. Results Interpersonal relationship regulating technique in the static state of Qigong is a specific and practical technique of SPT, which has been gradually standardized and been mature with more than two decades of innovation and accumulation. Conclusion Interpersonal relationship regulating technique in the static state of Qigong has great clinical value.

Objective To evaluate the causes of postoperative complications in severe liver rupture and explore the prevention experienees.Methods The clinical data of 66 liver rupture patients who underwent surgical treatment in our hospital from January 2005 to July 2012 were retrospectively analyzed.The commou complications after surgery and the treatment measures of the complications were sludied.Results There were 9 deaths of severe liver rupture after surgery and related complications were found in 22 cases,including bile leakage (5 cases),intrahepatic abscess (3 eases),subphrenic infection (3 cases),pleural effusion (8 cases),intra-abdominal hemorrhage (2 cases),stress ulcer bleeding (2 cases).Conclusions Using damage control theory could improve the cure rate.Precise liver transection and postoperative excellent management was able to reduce the incidence of postoperative complications in severe liver rupture.

ObjectiveTo evaluate the learning curve of utilizing the full endoscopic technique for the surgical treatment of lumbar disc herniation.MethodsFrom August 2008 to February 2009,30 patients with lumbar disc herniation underwent lumbar discectomy by the full endoscopic-only approach were retrospectively analyzed.The patients were divided into three groups of 10 sequential cases each.Group of early time consisted of the first 10 cases,Group of medium term the subsequent 10 cases,and Group of later time the last 10 cases.The clinical evaluation data included operative time,length of hospital stay,leg and back pain visual analogue scale (VAS),and complications.ResultsAll patients were observed prospectively for (1.61±0.22) years.There was no measurable intraoperative bleeding,no postoperative infections and symptomatic recurrences in the three groups.Compared to group of early time,the operative time in group of medium term was significantly decreased(P＜0.05).The patients in group of later time had much less operative time than that in group medium term(P＜0.05).There was no significant difference with length of hospital stay between the three groups (P＞0.05).The improvement of leg and back VAS in each group was similar:there was a significant improvement 3 months post-operation compared with pre-operation (P ＜0.05),but no statistical difference between 3 months post-operation and final foliow-up(P＞0.05).The complication rate was 12.5％ for group of early time,10％ for group of medium term,and 0 for group of later time.There were two cases conversed to an open procedure for group of early time compared with 0 cases in both other groups.ConclusionExcellent clinical and minimally invasive outcomes can be obtained in the surgical treatment of lumbar disc herniation via the interlaminar approach assisted by full endoscopic technique.However,attention must be paid to the steep learning curve with use of this complex technique.Obtaining microsurgical experience and suitable patient selection can help shorten the learning curve and decrease the complications.

Objective To evaluate the efficacy of sleep regulation technique of thought imprint psychotherapy in lower resistant state (TIP) for the treatment of primary insomnia.Methods 45 hospitalized outpatients with primary insomnia from sleep medical clinic of Guang'anmen Hospital were randomized at a 2 ∶ 1 ratio into a therapy group and a control group using random table.In the therapy group,patients received sleep regudation technique of TIP twice per week.In the control group,patients were asked to take zopiclone 3.75mg per night 30 minutes before bedtime.And the maximum dose can be 7.5mg according to their condition.The treatment lasted one month.Pittsburgh sleep quality index (PSQI) was used to assess sleep quality.Results ① After 4 weeks treatment,the clinical efficacy showed significant difference between the two groups (therapy group was 80％,control group was 33.3％).② Inner group comparison:therapy group was significantly improved in PSQI sleep quality (1.20 ± 0.71),sleep latency(1.53 ±1.01),sleep time(1.23 ±0.82),sleep efficiency(0.90±1.21),sleep disorders (1.30±0.65),sleep medication(0.13±1.36),daytime function (1.67±0.99),total score(8.30±3.19),(P＜0.05); the control group was significantly improved in the PSQI sleep quality(1.60±0.91),sleep latency(1.87±0.64),(P＜0.05).③ Inter group comparison:after treatment,compared to the control group,the therapy group was superior in the sleep latency,sleep time,sleep efficiency,sleep medication,daytime function,and total score ofPSQI (P＜0.05).Conclusion Sleep regulation techmique of thought imprint psychotherapy in lower resistant state can improve sleep quality of primary insomniacs.

Objective To introduce personality-reshaping technique in the static state of Qigong to enrich modern clinic psychotherapy. Methods Based on previous therapeutic experience, we introduce personality-reshaping technique in the static state of Qigong. Results Personality-reshaping technique in the static state of Qigong can change personality of patients through series of techniques. Conclusion Personality-reshaping technique in the static state of Qigong has great clinical values.

The paper introduced a therapeutic method of lower-resistant state therapy in the treatment of hysterical aphasia.This method was rapid and effective comparing to other therapeutic methods,showing great clinical values.

Objective To evaluate the effect of the high concentration of sevoflurane wash-in in induction of anesthesia on the cardiac function in young infants.Methods ASA Ⅰ young infants,aged 28-60 days,undergoing resection of hemangioma on body surface under general anesthesia,were studied.According to the principle of randomized block design,8 blocks were designed based on the body weight (3.0-3.4 kg,3.5-3.9 g,4.0-4.4 kg,4.5-4.9 kg,5.0-5.4 kg,5.5-5.9 kg,6.0-6.4 kg,6.5-6.9 kg),with 3 infants in each block.The infants were randomly divided into 3 groups (n =8 each):anesthesia induction with 6％,7％ and 8％ sevoflurane groups (groups S1-3).In groups S1-3,the infants inhaled 6 ％,7 ％ and 8 ％ sevoflurane respectively for induction of anesthesia.Transthoracic echocardiographic examinations were performed before sevoflurane inhalation (baseline,T1) and at 4 min of sevoflurane inhalation (T2) to record the stroke volume (SV),left ventricle end-diastolic volume (LVEDV),ejection fraction (EF),stroke volume index (SI),heart rate (HR),cardiac output (CO),cardiac index (CI),peak early diastolic velocity of mitral annulus (Ea),peak late diastolic velocity of mitral annulus (Aa),peak systolic velocity of mitral annulus (Sa),and Tei index.The mitral annulus poteroseptal Ea/Aa (Ea/Aa) was calculated.Results Compared with the baseline value,SV,EF,SI,Ea/Aa and Tei index were significandy increased,HR,Sa,Ea and Aa were significantly decreased (P ＜ 0.05),and no significant change was found in LVEDV,CO and CI at 4 min of sevoflurane inhalation in groups S1-3 (P ＞ 0.05).Tei index was significantly higher in groups S2.3 than in group S1 (P ＜ 0.05).There was no significant difference in the parameters of cardiac function between group S2 and group S3 (P ＞ 0.05).Conclusion 6 ％,7 ％ and 8 ％ sevoflurane wash-in in induction of anesthesia exert no effect on the cardiac function in young infants,and the 3 concentrations can be recommended for clinical use.

Will-powered technique training in the lower resistant state is an important therapy method of TCM psychology. The therapy refers to five aspects of improving cognitive and emotional function by using TIP technique, making imaginary training in the lowered resistant state, strengthening will-power training with situation and image technique, adopting information module imprinted technique and developing training technique. The content includes cultivating abilities in independence, resolution, firmness, and self-control. The technique has application value in clinic.

Objective To introduce cause-tracing technique in the static state of Qigong to enrich modem clinic psychotherapy. Methods Based on previous therapeutic experience, we reduced resistance from patients through interview.Then the cause of diseases was detected by analyzing implicit memory. Results Cause-tracing technique in the static state of Qigong could get important relevant information by excavating implicit memory. It was a cause-tracing method characterized by low-resistance and high-speed. Conclusion Cause-tracing technique in the static state of Qigong had great clinical value.

Objective To detect the influence of marOR mutations on antibiotic resistance in Shigella spp. Methods The marOR gene with four-base deletion was amplified by overlap PCR, then inserted in a T-vector and transformed into DH5α. The clone of marOR gene with four-base deletion and three point mutations was prepared from the strain having these mutations. Electrophoresis and sequencing were preformed to certify the correction of the cloned genes. Drug susceptibility tests were preformed for the strains harbouring the different clones [DH5α, DH5α (T), DH5α (marOR), DH5α (marOR-CATT), DH5α(marOR-CATT + 3m)]. Results Compared with the control strain (DH5α-T), the antibiotic resistances of marOR with four-base deletion [DH5α (marOR-CATT)] were higher to streptomycin, tobramycin, cefazolin and cefalexin, and the antibiotic resistances of marOR with four-base deletion and three point mutations [DH5α (marOR-CATT + 3m)] were higher to streptomycin and to tetracycline. The antibiotic resistances of DH5α (marOR-CATT) and DH5α (marOR-CATT +3m) to streptomycin, tetracycline, chloramphenicol, cefazolin, levofloxacin, ciprofloxacin and norfloxacin were higher than DH5α (marOR). The diameters of the antibiotics except the trimethoprim between DH5α (marOR-CATT) and DH5α (marORCATT +3m) had not significant disparity. Conclusion The four-base deletion in 1376-1379 sites of the marOR gene increased the resistance of Shigella spp to some antibiotics. The point mutations in 1411, 1417,1435 sites of the marOR gene have little influence on the antibiotic resistance of Shigella spp.