Purpose: This study is to observe the therapeutic effect of puncturing acupoints Dazhui (GV 14), Fengchi (GB 20), Jiaji (Ex-B 2), Waiguan (TE 5), Qiuxu (GB 40) and Zhaohai (KI 6) on vascular headache. Methods: 68 cases of vascular headache were divided into treatment group including 38 cases and control group including 30 cases. The patients in treatment group were treated by puncturing Dazhui (GV 14), Fengchi (GB 20), Jiaji C26 (Ex-B 2),Waiguan (TE 5), Qiuxu (GB 40) through Zhaohai (KI 6) and patients in control group were asked to take Pizotifen orally. Results: After 2 courses of treatments, 35 cases got effect in treatment group, and 22 cases got effect in control group, the therapeutic effect in treatment group was superior to that in control group (P＜ 0.05). Conclusions: Puncturing Dazhui (GV 14), Fengchi (GB 20), Jiaji C26(Ex-B 2), Waiguan (TE 5), Qiuxu (GB 40) and Zhaohai (KI 6)is effective for the vascular headache.

With reference to medical personnel's infection during the prevalence of SARS, the paper offers some general information on the risks of occupational exposure of medicine. The commonly seen risks fall into four major categories, including biological risks, such as infection of hepatitis B, hepatitis C, HTV, etc.; chemical risks, such as those from antineoplastic drugs, cleaning agents, disinfectants, narcotics, dusts; physical risks, such as radiation, injury by sharp instruments, injury from heavy burden, forced body positions, etc.; and psychological risks. The paper also puts forward some proposals for safety protection: intensifying training in occupational safety of medicine, reinforcing the protection of high-risk groups of people and high-risk body parts, augmenting the supply of protective materials and the improvement of protective facilities, and setting up and perfecting long-acting protection and supply mechanisms.

Objective To research the effects of alprostadil on nerve conduction velocity and blood flow status of 120 patients with type 2 diabetic peripheral neuropathy.Methods 120 cases of patients with type 2 diabetic peripheral neuropathy in our hospital were selected as the research objects.According to the hospital admission,they were divided into observation group and control group.The control group was treated with Mecobalamin intravenous drip treatment; on the basis of the above treatment,the observation group was treated with alprostadil intravenous drip treatment,and the treatment time was two weeks.To established the relevant clinical curative effect standard for evaluated and compared the clinical therapeutic effect of the two groups.Before and after treatment,compared the nerve conduction velocity and blood flow between the two groups.Results The total effective rate of the observation group was 86.67％,and the control group was 71.67％,the difference was statistically significant(P<0.05).Before treatment,there were no significant differences between the two groups in nerve conduction velocity and blood rheology indexes.After treatment,the median nerve MNSV,common nerve MNSV,median nerve SNCV,common nerve SNCV of the observation group were(54.14±7.29)m/s,(50.22±7.85)m/s,(48.47±7.64m/s and(47.04±6.58m/s respectively,were significantly higher than the control group(50.11±6.85)m/s,(46.24±7.08)m/s,(44.58±7.21)m/s,(43.57±6.55)m/s.The observation group of plasma viscosity,high shear viscosity and low shear viscosity levels were(2.48±0.29)Mpa/s,(4.87±0.41)Mpa/s,(33.46±2.77)Mpa/s,were significantly lower than the control group(2.83±0.33)Mpa/s,(5.23±0.44)Mpa/s,(34.83±2.54)Mpa/s,and the differences between the groups were statistically significant(P<0.05).Conclusion Alprostadil can significantly improve nerve conduction velocity and blood flow condition in patients with type 2 diabetic peripheral neuropathy,has better clinical curative effect.

Objective To describe the characteristic CT imaging of adenomyomatosis of gallbladder and to improve the diagnostic knowledge of the disease. Methods Eleven (5 males and 6 females, mean age = 43 years) patients with adenomyomatosis of gallbladder (4 diffuse, 2 segmental, and 5 fundal) confirmed by histopathology and performed by CT examination including plain, dynamic or multiphase enhancement and / or oral cholegraphic agent were reviewed. Results Correct diagnosis was made in 7 of 11 patients(3 diffuse, 1 segmental, and 3 fundal). CT findings included segmental or diffuse gallbladder wall thickening in the involved area, associated with well defined and regular borders at both of the inner and outer layers. Significant CT findings of this entity were marked enhancement of wall layers during triphase contrast CT scanning, especially at delayed scans. Rokitansky Aschoff sinuses were clearly shown by CT, presenting as characteristic "aureola " sign, when performed with application of a cholegraphic agent. Conclusion Understanding the CT findings and using the optimal methods, we can use CT scan as a valuable method to help make the diagnosis in most cases of adenomyomatosis.

Objective To explore the epidemiological features of the metabolic syndrome in a cohort study of Xiangtan Mengkuang in China.Methods The prevalence rate,means and standard deviation of various risk factors associated with metabolic syndrome were analysed on the basis of base-line survey of risk factors of 5792 subjects(aged 25 years-old～) in 2004.Results (1)The prevalence rate of metabolic syndrome was 12.9%(12.4% in males,and 13.7% in females,P

To obtain the aptamers with high affinity and specificity for cyclosporin A(CsA),a synthesized 78 nt single stranded DNA(ssDNA)random library containing 35 random sequences flanked by invariant primer was subjected to 1 1 rounds of selection against CsA by SELEX protocol.Magnetic beads were used for target immobilization and the biotin-streptavidin-horseradish peroxidase system was employed for determining the binding affinity between the aptamers and CsA. After ten rounds of selection and amplification, with an increasing affinity for each round,the selected aptamers were cloned,sequenced and analyzed for their primaryand secondary structures.The 19 aptamers were divided into five groups based on primary sequence homology.Hairpin loop is the main motif in the predicted secondary structure and is supposed to be the binding part of the aptamers to CsA.The CsA-specific aptamers will be useful for enzyme-linked assays or immunofluorescence asses of CsA.

AIM: AIM: To explore the relationship between apoptosis in the lung tissues and lung ischemia/reperfusion injury, and observe effects of panax notoginseng saponins (PNS) on apoptosis in lung ischemia/reperfusion injury. METHODS: Single lung in situ ischemia/reperfusion animal model was used. Eighty four Japanese white rabbits were randomly divided into control group (control), ischemia/reperfusion 1 h group (IR1h), IR3h, IR5h, Panax Notoginseng Saponins 1 h group (PNS1h), PNS3h and PNS5h. TUNEL, immunocytochemistry and in situ hybridization techniques were used to observe apoptosis and Fas/FasL expression in various phases of lung ischemia/reperfusion. RESULTS: Cell apoptosis in lung tissues were significantly high, Fas/FasL mRNA and its protein were up-regulated in lung tissues of lung ischemia/reperfusion injury compared with control (all of P

Objective To investigate the efficacy and safety of catheter radiofrequency ablation of paroxysmal supraventricular tachycardia.Methods From Jul 2003 to Jan 2011,1106 cases with narrow QRS complex tachycardia who were treated by catheter radiofrequency ablation were recruited from our center and followed up for the rates of successful treatment,rcurrence and complications.Results There were in total 1106 patients (atrioventricular reentrant tachycardia:588 ; atrioventricular nodal reentrant tachycardia:477; atrial tachycardia:41 ),with a sex proportion of 1∶1.Successful ablation rate was 98.3 ％ (1087/1106).Of the 1087 successful ablation cases,43 (3.9％ )were warranted repeated ablation.The recurrent rates for trioventricular nodal reentrant tachycardia,atrioventricular reentrant tachycardia,left accessory pathway and right accessory pathway were 1.5％,5.6％,3.9％,and 9.1％ respectively.Complication rate was 1.5％.The major complications included pheumothorax ( 6 cases ),pulmonary embolism ( 1 case ),transient third-degree atrioventricular block ( 2 cases ),first-degree atrioventricular block ( 3 cases ),and persistent third-degree atrioventricular block(2 cases)with pacemaker implantation.There was one case of cardiogenic sudden death 5 days after the treatment procedure.The cause of his death was chronic stroke-related but not related to the operation procedure.Conclusion Catheter ablation has high efficacy and low complication rate in long-term follow-up,and is a promising treatment for paroxysmal supraventricular tachycardia.

Objective The expression of chemokine C-X-C motif ligand 13 (CXCL13) within liver in primary biliary cholangitis (PBC) patients is significantly increased, but its origin and mechanism is not clear yet.The study aimed to investigate the expression of CXCL13 in the liver of mice through establishing a mouse model of PBC.Methods C57BL/6 mice were randomly divided into experiment group (n=20) control group(n=10).The mice in the experimental group were intraperitoneally injected with polyriboinosinic polyribocytidylic acid (Poly I:C) while the mice in control group were injected with PBS of the same volume.The level of serum AMA was quantified by ELISA and intrahepatic inflammatory cells were assessed by HE staining.Kupffer cells, liver sinusoidal endothelial cells, and infiltrating lymphocytes in the liver of mice were collected by in situ perfusion enzyme digestion and magnetic bead separation methods.The transcriptional level of intrahepatic CXCL13 in liver tissues and cell subpopulations were detected by qPCR.Results The serum AMA titers of the mice in experiment group increased gradually with the prolonging of modeling time and the positive rates at the 4th, 8th, and 12th week after the first injection of Poly I:C were 5.9%, 52.9% and 76.5% respectively.While the serum AMA titers of the mice in control group were at a lower level through the modeling process, with only 2 mice presenting a little higher level above positive cutoff value at the 12th week.The results of HE staining in liver tissues of both groups showed that there were a great amount of intensely infiltrating inflammatory cells in the mice of experimental group while no inflammatory cell infiltration were found in the mice of control group.The separation purity of Kupffer cells and liver sinusoidal endothelial cells in the mice of experiment group tested by flow cytometry were 76%-80%, 68%-72% respectively.Compared with the CXCL13 mRNA level in Kupffer cells [2.34(0.22-8.64)], the expression levels in liver sinusoidal endothelial cells and infiltrating lymphocytes declined[0.27(0.03-1.64), 0.05(0-0.22), P<0.05].Conclusion The chemokine CXCL13 is predominantly produced by Kupffer cells in the liver of PBC mice established by Poly I:C injection.