Objective To investigate the dynamic CT features of peripheral lung cancer (PLC) and pulmonary inflammatory pseudotumor(IPT).Methods 23 patients with PLC and 6 patients with IPT were undergone plain and dynamic enhanced CT scans (beginning at 30 s after the onset of injection,serial scan were obtained at 30 s intervals within 180 s) before surgery or administration of antibiotic.Results The average CT values of plain CT,CT enhanced attenuation at 30 s and 90 s were significantly different between PLC and IPT.There were no significant decline following peak enhancement in IPT but PLC within 180 s.Conclusion The comprehensive analysis of plain CT value,enhanced CT value at 30 s,and the decreasing rate after 90 s of administration of contrast material may be useful in differential diagnosis between PLC and IPT.

Objective To study the relationship between expression of p16 and tumor angiogenesis as well as value of CT enhancement in peripheral lung cancer. Methods twenty-three patients with peripheral lung cancer were examed with dynamic CT scan before surgery.The MVD、protein expression of VEGF and p16 were measured immunohistochemically on speciments of resected tumors.Results There were negative correlation tendency between expressions of p16 and VEGF,but the difference were no significant.During the course of delaying 30 s from injecting contrast dose,the relating coefficient was the highest between enhanced CT value and MVD,P=0.9364.The mean value of MVD and CT enhancement in the course of delaying 30 s in p16 negative expression group were higher than that in the group of p16 positive expression with no significant.Conclusion p16 negative expression may improve angiogenesis in peripheral lung cancer.

BACKGROUND:Hair fol icle stem cel s derived from skin and hair have the most readily available sources, and cannot lead to serious complications and immunogenicity after col ection, which can be used for autologous transplantation. OBJECTIVE:To provide effective experiment basis for hair fol icle stem cel s in the repair of skin wound through reviewing the role of hair fol icle stem cel s in promote the repair of skin wound. METHODS:Articles related to hair fol icle stem cel s for the repair of skin wound were retrieved in CNKI and PubMed databases (1999-01/2014-12). The key words were“hair fol icle stem cel , skin repairing, regenerative medicine, tissue engineering”in Chinese and English, respectively. A total of 45 articles based on inclusion criteria and exclusion criteria were obtained for the review. RESULTS AND CONCLUSION:Hair fol icle stem cel s belong to adult stem cel s, which are easy to access, have a significant number, and possess differentiation potential and safety. Hair fol icle stem cel s can promote the repair of skin wound and provides good seed cel s for regenerative medicine and tissue engineering research through early vascularization of the skin, epidermal and appendage regeneration, signaling pathways, and transcription factors.

Objective To investigate and summarize the clinical and high-resolution computed tomography(HRCT) characteristics of invasive pulmonary fungal infections(IPFIs)in children.Methods Clinical and HRCT data of 35 cases with IPFIs admitted in our hospital between March 2007 and July 2015 were retrospectively analyzed.The clinical and HRCT characteristics were summarized.Results Thirty-five patients consisted of 23 boys and 12 girls with mean age of(3.2±1.9) years.Host factors included acute leukemia (n=12),primary immunodeficiency disease (n=4),congenital heart disease (n=2),cerebral palsy (n=2),severe influenza A infection (H1N1) (n=2),ichthyosis (n=1),acquired immunodeficiency syndrome(n=1),systemic lupus erythematous (n=1),tubercular meningitis(n=1),mechanical ventilation(n=2).All patients were treated with broad-spectrum antibiotic,ranking by descending order:third-generation cephalosporins (28 cases),carbapenems(19 cases)and vancomycin (18 cases).Seventeen cases were treated with corticosteroids systemically and 12 cases with acute leukemia took antineoplastic medicine.The symptoms of IPFIs were intermittent or persistent fever,cough and rales.HRCT results:nodules (n=25,71.4％),subpleural patchy opacities (n=24,68.6％),mass (＞3cm) (n=4,11.4％),halo sign (n=27,77.1％),cavities (n=8,22.9％),air crescent sign (n=4,11.4％),miliary nodules (n=2,5.7％),pleural effusion (n=14,40％).Conclusion There are certain specific characteristics of IPFIs in children in clinical and HRCT aspects.The possible diagnosis of IPFIs can be made based on clinical and HRCT features.

Objective To investigate the effects of 810 nm semi-conductor laser irradiation on the proliferation of olfactory ensheathing cells in vitro. Methods Olfactory ensheathing cells obtained from adult rat olfactory mucosa using the method based on different rates of attachment were irradiated with a semi-conductor laser ( wave length 810 nm; power density 10.3 mW/cm2) for 30, 60 or 120 seconds. Laser irradiation was performed 3 times with a 24 h interval. After the last irradiation, the cells were cultured. At the 3rd, 5th and 8th day of cell culture,cell proliferation was assessed with cell counts and a methylthiazoletetrazolium ( MTT) colorometric method. Results After 3 days of cell culture, the number of cells and average MTT values showed no statistically significant difference between the irradiated and control groups. At the Sth and 8th day, the differences among all the laser exposure groups and with the control group were significant, except for the average MTT values of the control group and the 30 s exposure group. Maximal effect was achieved with a 60 s exposure. Conclusions Low power laser irradiation can stimulate the proliferation of olfactory ensheathing cells in vitro, and the effect is time-dose dependent. The optimal irradiation time was found to be 60 s daily for 3 times, with a 24 h interval.

BACKGROUND: In clinical practice, the selection of dental implants with different specifications is based on the patient' s mandibular plane angle, and the long-term success rate is different in dental implants with different specifications. Therefore, it is reasonable to assume that the mandibular plane angle could affect the mandibular stress distribution of dental implants, thus affecting the success rate of dental implantation.OBJECTIVE: To perform an orthogonal experimental study on the mechanical effects of different implant specifications, mandibular plane angles and mandibular bone densities on stress distribution of the first mandibular molar implants.METHODS: A L9(33) orthogonal experiment was designed with 3 three-level factors, including mandibular plane angle (14°, 22°, 34°), implant diameter (6.6, 8.2, 9.6 mm), and mandibular bone density (types II, III, IV). Different dental implants with different combinations were implanted into an alveolar bone model, and placed onto a pressure testing machine under 500 N load. Then, strain (stress) data were collected in real-time and analyzed.RESULTS AND CONCLUSION: The optimal combination was as follows: low angle; 4.8 mm in diameter; mandibular bone with type II bone density. The relations of all the three factors were the diameter of dental implant > the mandibular plane angle > mandibular bone density. To conclude, the mandibular plane angle has some certain influences on the stability of dental implants. If the biting force is the same, dental implant bears the largest stress under the high angle,subsequently followed by the average angle and the low angle.

Objective　To investigate the effects of nifedipine, a calcium channel blocker, on cellular proliferation in gingival fibroblasts in vitro. Methods　Gingival fibroblasts were cultured and incubated with various concentrations of nifedipine (1 200.0, 360.0,108.0 and 32.4 μg/L) for up to 7 days. 3H-TdR incorporation assay in vitro taken was used to evaluate the amount of DNA synthesis. Results　Cell numbers were significantly different among these groups on sixth and seventh day, especially higher in 1 200.0 μg/L and 360.0 μg/L groups. The incorporation percentage in high concentration groups were significantly higher than those in others. Conclusion　Nifedipine can influent proliferation of gingival fibroblsts in high concentration.

Objective To detect EPPB41L3 methylation frequency difference between esophageal squamous cell carcinoma (ESCC) tissues and the normal tissues and between ESCC patients′plasma and healthy volenteers′plasma, and to analyze the correlation with clinicopathological parameters. Methods We collected esophageal squamous cell carcinoma tissues (n = 42 patients) and adjacent surrounding normal tissues (n = 42 patients), and plasma from 42 patients with ESCC and from 50 healthy individuals. We used methylation specific PCR (MSP) combined with agarose gel electrophoresis to detect the methylation status of the EPB41L3. We used the SPSS 13.0 software for statistical analysis by χ2 test and Fisher′s exact test. Results EPB41L3 frequency of methylation was significantly higher in tumor tissues than in the adjacent tissues (59.5% vs. 4.8%), the difference was statistically significant (χ2 = 28.873, P < 0.001). For plasma, EPB41L3 methylation frequency was 31.0%in cancer patients, while was not detectable in the healthy volunteers. Methylation of EPB41L3 in tissues was more frequently found in patients with tumor size of ≥ 5 cm or T3 than in patients with tumor size of < 5 cm or T1-2. Conclusions The methylation frequency of EPB41L3 is higher in ESCC tissues than in control normal tissues, and higer in plasma from ESCC patients than that from the healthy volunteers. EPB41L3 methylation is more frequently found in patients with more advanced disease.

Objective To investigate the protective effect of protocatechuic acid(PCA)on the PC12 cell model of Alzheimer’s disease(AD)and to explore its mechanism . Methods Amyloid beta peptide 1-42(Aβ1-42)fiber polymers were identified by immunofluorescence. After PC12 cells were stimulated with the Aβ1-42 fiber polymers, the cellular morphology was observed at different time points of hour 0, 3, 6, 9, 12, 24 , and the cellular viability was tested by methyl thiazolyl tetrazolium(MTT)assay to monitor the modeling condition. The effect of PCA on PC12 cells was detected after PC12 cells were pretreated with the different contentions of PCA. Autophagy-related marker Beclin1 protein level was detected by Western blotting method to investigate the protective mechanism of PCA. Results Aggregated white Aβ1-42 mass was stable at hour 12 and 24, and showed no significant difference between the two time points, the cell damage rate being 40%. Therefore, we defined culturing time being 12 and 24 hours as the modeling condition of AD model. The cell viability was increased with 200-800 μmol/L of PCA after culturing for 24 hours(P<0.01) , and the Western blotting results showed that the Beclin1 protein expression was up-regulated by PCA. Conclusion PCA prevents PC12 cells from Aβ1-42-induced toxicity, the mechanism being related with the increase of cellular autophagy.

Objective The expression of chemokine C-X-C motif ligand 13 (CXCL13) within liver in primary biliary cholangitis (PBC) patients is significantly increased, but its origin and mechanism is not clear yet.The study aimed to investigate the expression of CXCL13 in the liver of mice through establishing a mouse model of PBC.Methods C57BL/6 mice were randomly divided into experiment group (n=20) control group(n=10).The mice in the experimental group were intraperitoneally injected with polyriboinosinic polyribocytidylic acid (Poly I:C) while the mice in control group were injected with PBS of the same volume.The level of serum AMA was quantified by ELISA and intrahepatic inflammatory cells were assessed by HE staining.Kupffer cells, liver sinusoidal endothelial cells, and infiltrating lymphocytes in the liver of mice were collected by in situ perfusion enzyme digestion and magnetic bead separation methods.The transcriptional level of intrahepatic CXCL13 in liver tissues and cell subpopulations were detected by qPCR.Results The serum AMA titers of the mice in experiment group increased gradually with the prolonging of modeling time and the positive rates at the 4th, 8th, and 12th week after the first injection of Poly I:C were 5.9%, 52.9% and 76.5% respectively.While the serum AMA titers of the mice in control group were at a lower level through the modeling process, with only 2 mice presenting a little higher level above positive cutoff value at the 12th week.The results of HE staining in liver tissues of both groups showed that there were a great amount of intensely infiltrating inflammatory cells in the mice of experimental group while no inflammatory cell infiltration were found in the mice of control group.The separation purity of Kupffer cells and liver sinusoidal endothelial cells in the mice of experiment group tested by flow cytometry were 76%-80%, 68%-72% respectively.Compared with the CXCL13 mRNA level in Kupffer cells [2.34(0.22-8.64)], the expression levels in liver sinusoidal endothelial cells and infiltrating lymphocytes declined[0.27(0.03-1.64), 0.05(0-0.22), P<0.05].Conclusion The chemokine CXCL13 is predominantly produced by Kupffer cells in the liver of PBC mice established by Poly I:C injection.