Objective To study the effects of repetitive transcranial magnetic stimulation (rTMS) on rats'locomotor function after hemisectional spinal cord injury (SCI). Methods A model of hemisectional SCI was established at the T10 segment in 12 rats,which were then randomly divided into an SCI-rTMS group and an SCI-control group.Another 6 rats served as normal controls.The SCI-rTMS group received threshold rTMS daily for 4 weeks,while the SCI-control group was given sham rTMS.The normal control group was left without any treatment.BassoBeatti-Bresnahan (BBB) locomotor scores were recorded weekly.Motor evoked potential (MEP) was detected at the 28th day after the operation.Neurofilament-200 (NF-200) was detected with immunofluorescent staining of the spinal cord lesions. Results The BBB scores in the SCI-rTMS group improved more than those in the SCI-control group.MEP of the fight hindlimb was detected in the rTMS group,but not in the control group.Expression of NF-200 marker increased significantly more than in the rTMS group. Conclusions rTMS can improve locomotor function of rats after spinal cord hemisectioning,which may result from increased expression of NF-200 and the regeneration of axons.

Objectives To investigate the regularity of fluctuation and clinical senses of serum c reactive protein (CRP) level in patients with acute cerebral infarction (ACI) Methods 100 subjects (56 males and 44 females) were divided into five groups including 24 large artery arteriosclerosis (LAA), 27 small artery occlusion (SAO), 2 cardiac embolism(CE), 34 other Certain(OC) and 13 undetermined etiology according to Trial of ORG 10172 in Acute Stroke Treatment (TOAST) Through enzyme linked immunoabsorbent assay (ELISA), we detected and analyzed CRP levels of 100 inpatients Results The risk factors of stroke included coronary heart disease , hypertension, diabetes and intoxication of CO and so on Among the above mentioned, the level of CRP and rate of positive CRP of patients who poisoned by CO was the highest That of coronary heart disease group was lower However, there was no notable difference between hypertension and diabetes groups Conclusions CRP may play a certain role in the occurrence and development of ACI It represents different levels in different types of stroke

Objective To investigate the syndromes, syndrome elements and target sites of diabetic cerebral infarction. Method Firstly, literatures were searched from January 2003 to October 2013 in China Academic Journals Database (CNKI) retrieval system, then a database of 22 eligible articles was established. Syndromes of diabetic cerebral infarction were extracted from the database, then syndromes, syndrome elements and target sites were analyzed. Results Totally 2550 cases of diabetic cerebral infarction were concerned with 15 syndromes, including 659 cases of stagnation of phlegm and blood (25.84%), 445 cases of stagnation of phlegm-heat (17.45%), 366 cases of wind-phlegm syndrome (14.35%) and 341 cases of qi deficiency and blood stasis (13.37%). There were 10 syndrome elements in the 2550 cases, of which 1588 cases with phlegm (62.27%), 1026 cases with blood stasis (40.24%), 649 cases with qi deficiency (25.45%) and 539 cases with yin deficiency (21.14%). And seven target sites were encompassed, the highlights of which included 2502 cases of liver (98.12%), 2194 cases of spleen (86.04%), 2096 cases of kidney (82.20%) and 1026 cases of brain (40.24%). Conclusion The important syndrome elements of diabetic cerebral infarction were phlegm, blood stasis, qi deficiency and yin deficiency. The four major syndromes comprising stagnation of phlegm and blood, stagnation of phlegm-heat, wind-phlegm syndrome and qi deficiency and blood stasis were concluded, mainly affected the liver, spleen, kidney and brain. And there were two fundamental pathogenesis in diabetic cerebral infarction:qi and yin deficiency, phlegm and blood stasis.

Objective To investigate the change of plasma cholinesterases (CHEs) in the people with diabetes or fatty liver or overweight , and explore the role of CHE in these diseases .Methods The plasma CHEs in 2834 subjects were detected , and these subjects were divided into five groups , including diabetes , fatty liver , overweight , diabetes with fatty liver , and the normal groups . Results The plasma CHE activities in diabetes group , fatty liver group , overweight group , and diabetes with fatty liver group were all higher than the normal group [(8943 ±1896)U/L, (9716 ±1673)U/L, (8798 ±1710)U/L, (9385 ±1687)U/L vs (8028 ±1621) U/L], and the CHE level in the fatty liver group was highest among five groups .However, the CHE level in diabetes group or fatty liv-er group was not significantly different from that in the diabetes with fatty liver group .The CHE level of the people with components of metabolic syndrome (MS) was significantly higher than that without MS component [(8786 ±1514)U/L, (9141 ±1771)U/L, (9705 ±1628)U/L, (9138 ±1768)U/L, (9530 ±1607)U/L vs (7821 ±1324)U/L]),but the CHE level was not increased gradually with the increased MS component.The plasma CHE had a negative correlation with age ( P =0.00),but it had a positive correlation with triglyceride (TG), total cholesterol (TC), and body mass index (BMI)( P =0.00).Conclusions The plasma CHE activity was el-evated in diabetes group , fatty liver group , and overweight group , which might be a risk factor in these diseases .Controlling the plas-ma CHE might help to treat the metabolism diseases .

To explore indications regularity of analogous decoctions of Erchen Decoction.323 analogous decoctions of Erchen Decoction were found in Concise Thesaurus of TCM Formula.Diseases and symptoms of TCM were analyzed.22 diseases and 118 symptoms of TCM were found.The main indication of analogous decoctions of Erchen Decoction included spleen-stomach and lung system,other symptoms were broadly involved.Erchen Decoction was the most appropriate for phlegm.

Objective To investigate the cellular fragments and particle image changes of inventory aleucocytic suspending RBC produced by the storage time extension ,RBC damage or hemolysis in order to provide the revelatory experimental basis for the transfusion safety .Methods The supernatant was prepared from different stored days (3 ,7 ,14 ,21 d) of stock aleukocytic suspen‐ding RBC .The particles in supernatant were observed and morphologically analyzed by using the microscopic static image analytic technology .Results There were a small amounts of visible particles in the sample supernatant preserved for 3 ,7 d and the parti‐cles′sizes are similar to cells′;the number of particles began to significantly increase from 14 d and the diameter became smaller . The particles filled the entire field until 21 d ,showing fragmentary status .Conclusion The cellular fragments and particles in the supernatants of stock aleukocytic suspending RBC with the storage period exceeding 14 d are significantly increased and have signif‐icant difference compared with those stored for less than 14 d .These exogenous fragments and particles may become antigens and induce the body immune response ,lead to transfusion adverse reactions .It is recommended that the patients should be transfused with stock aleukocytic suspending RBC within a storage period of 14 d .

The promyelocytic leukemia (PML) protein is an important part of the PML nuclear bodies (PML-NBs) structure.PML protein is crucial for the assembly of PML-NBs and recruits more than 30 different proteins, including DAXX, ATRX, and small ubiquitin-like molecules involving SUMO to the PML-NBs region.Increased evidence has emerged that a number of different proteins is involved in regulating PML activities by post-translational modifications, such as SUMO modification, ubiquitination and phosphorylation.Here, we review recent studies on the combination of PML and different proteins in the process of apoptosis, replicative senescence and DNA damage response.

Objective To observe the expression of cytotoxicity and receptors on NK cells in breast cancer,and investigate the impact of soluble MICA(sohble MHC class Ⅰ-related molecules A,sMICA) on NK cells receptors expression and cytotoxicity.Methods ELISA was used to examine the sMICA in peripheral blood.The expressions of activated receptor(NKG2D),killer inhibitory receptor (KIR)(CD158b) and NK cells were identified by flow cytometry(FCM).Cytotoxicity of NK cells to breast cancer were tested by MTT.Results sMICA was (205.36±71.27)ng/L in breast cancer patients and 81.6 % samples were detected.There were positive correlations between sMICA levels with breast cancer stages.There were no difference of NK cells percentage between breast cancer and healthy person.The cytotoxicity of NK cells and expressions of NKG2D were obviously lower in breast cancer with sMICA(+) than in healthy person,but CD158b was higher in healthy person.After cultured with sMICA,NK cells cytotoxicity decreased from(76.2±6.7)% to(48.4±4.1)% and the expression of NKG2D reduced from(92.5±7.1)% to (62.5±6.4)%,but the the expression of CD158b increased from(10.6±3.2)% to (43.6±3.4)%.IL-15 up regulated the expression of NKG2D and NK cells cytotoxicity,but decreased the expression of CD158b by co-culturation with IL-15 and sMICA in sMICA+ patients with breast cancer.Conclusion sMICA reduced the expression of NKG2D and increased the expression of Kill,which lead to the down regulation of NK cells cytotoxicity.IL-15 can reverse this effect.

Objective To measure the equity of resources allocation in obstetric healthcare in Zhejiang Province, and provide evidence for decision making by healthcare departments of the government. Methods Lorenz curve and Gini coefficient were used to measure the equity of obstetric healthcare resources. Results The investigation found such allocation as equitable as measured by the layout of the number of live births, as the Gini coefficient of the distribution of obstetric doctors, nurses and beds was 0.1697,0.1971 and 0.1497 respectively by total birth, failing within the equitable range. However, when measured by geographic distribution, such coefficient were 0.2701, 0.3216 and 0.3341 respectively in cities, which were less equitable yet still within equitable range. Conclusions The government and health bureaus are recommended to scientifically predict the number of immigrants and rationally deploy healthcare resources according to the service radius of healthcare resources;moreover, more gynecologic doctors and nurses should be trained to improve the human resource structure in gynecologic departments. These effort can cater to the growing healthcare demands of the society resulting from a growing population of immigrants.

Objective To observe the effects of zinc-deficiency(ZD)on antioxidation system and repair of DNA damage and of hepatic cells by establishing an aging mice model induced with D-galactose. Methods Seventy 3-month-old male mice were divided randomly into 5 groups: normal young group, aging model group, aging+ZD group, aging+pair-fed group, aging+ZS group. The aging model were established with D-galactose (100mg/kg bw) by nap subcutaneous injection, while the young control group were injected with normal saline in the same way which lasted 30 days. Aging+ZD group and aging+ZS group were fed with ZD food(zinc 1.61?g/kg), others with normal food(zinc 50?g/kg). ZS food (zinc 100?g/kg)were given to aging+ZS group for the last 2 weeks in the course. On the 30 th day, all mice were killed by removing eyeballs and samples were collected immediately for later indexes detection. Results Compared with the aging control group, zinc contents and SOD activity of serum and liver decreased significantly, while MDA in the serum and liver and lipofuscin of liver increased much more in ZD mice. Comet assay also showed that the DNA damage of hepatic cells were more serious in aging ZD mice than in the control mice. The ratio of tail length/comet cell length in group ZD also increased significantly. However, moderate zinc supplement significantly improved all those indexes. Conclusions Zinc can affect the speed and degree of aging significantly. Zinc deficiency accelerates the aging progress; while moderate zinc supplement seems to be beneficial for retarding aging progress.