1.Application of gasless laparoscopic techniques in the treatment of abdominal trauma
Changsong ZHAO ; Defang PENG ; Wei ZHANG
Chinese Journal of Minimally Invasive Surgery 2001;0(04):-
Objective To study the role of gasless laparoscopic techniques in the treatment of abdominal trauma. Methods A retrospective analysis was made on clinical data of 52 cases of abdominal trauma considered unfit for pneumoperitoneum treated by gasless laparoscopic surgery. Results Prompt diagnosis and treatment were achieved in 43 cases. A conversion to laparoscopic exploration under pneumoperitoneum was required in 5 cases. The remaining 4 cases of severe injuries of the spleen and the bowel were cured by a conversion to open surgery after the determination of the incision site under gasless laparoscope. There were no fatal cases in the study. Conclusions Use of gasless laparoscopic techniques for the diagnosis, treatment and determination of incision site in patients with abdominal trauma considered unfit for pneumoperitoneum is feasible. It is a necessarily additional alternative to traditional laparoscopic surgery.
2.Determination of Ephedrine in Lianma Spraying by HPLC
Wei ZHAO ; Xuefeng HUANG ; Songfeng PENG
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(12):-
Objective To establish a method for determination of ephedrin in Lianma Spraying by HPLC. Method The analysis was carried out on C18 column (4.6 mm?200 mm, 5 ?m) by HPLC, the mobile phase was NaH2PO3-CH3OH-CH3CN-C3H15N (600∶320∶80∶1), the flow rate was 1.0 mL/min, the column temperature was 30 ℃, and detective wavelength was 207 nm. Result The linear range of ephedrin was 0.031 2~0.187 2 mg/mL, the average recovery rate was 98.94%, RSD was 0.8%. Conclusion The method is simple, rapid, accurate and reliable, and can be used to control the quality of the products.
3.Study of DPC Induced by Liquid Formaldehyde in the Liver and Testicles of Mice
Guangyin PENG ; Xu YANG ; Wei ZHAO
Journal of Medical Research 2006;0(11):-
0.05)and could be induced significantly after 12 h (P
5.Clinical study of the emodin thermosensitive hydrogel in the treatment of chronic periodontitis
Bing ZHAO ; Quan ZHAO ; Wei PENG ; Yu MU
Chinese Journal of Primary Medicine and Pharmacy 2010;17(16):2165-2167
Objective To evaluate the clinical effects of scaling and root planning combined with application of the emodin thermosensitive hydrogel on periodontitis. Methods Forty patients with chronic periodontitis with a total of eighty teeth were included in the study.For each patient after scaling and root planning,the teeth on one side were assigned as the test group and treated with the emodin thermosensitive hydrogel in the pocket once a week for four weeks.The teeth on the other side were assigned as the control group.All the clinical parameters were recorded at the baseline,the 6th week and 12th week after the treatment. Results At the baseline,there was no difference in the clinical parameters between the test group and the control group(P>0.05).A statistically significant improvement of all clinical parameters was observed in both groups 6w later(t = 39.06、62.76、20.50、8.05、158.32、31.45、46.53、8.43、8.08、8.03)(P<0.01=,and all clinical parameters of the test group were better than those of the control group,the differences were statistically significant(t = 31.50、27.91、3.92、7.38、20.09)(P<0.01=. Conclusion The emodin thermosensitive hydrogel could effectively control the symptoms of periodontitis.
6.Comparison of dosimetry in radiotherapy for craniospinal irradiation
Wei ZHANG ; Zhao MA ; Peng SHAO ; Wei JIANG ; Yumin GUAN
Chinese Journal of Radiological Medicine and Protection 2013;33(5):519-523
Objective To explore the dosimetry of simplified intensity modulated radiotherapy (sIMRT)in the spinal component of craniospinal irradiation(CSI)compared with conventional plan(3D-CRT).Methods Five previously treated patients were adopted to generate 3D-CRT,3-and 5-field slMRT plans.The prescribed dose was 36 Gy to the planning target volume(PTV)in 20 fractions.The dose distributions of target volume and normal tissues,and homogeneity index(HI)were ananlyzed using the dose volume histogram.The total monitor units(MUs)were also analyzed to compare the delivery time indirectly.Results For 3D-CRT plans,the region of the neighbouring fields only received 70% of the prescribed dose,and the maximum dose delivered in the normal tissues was increased to approximately 140% of the prescribed dose.The homogeneity index(HI)of 3-and 5-field sIMRT were 0.09 ± 0.01 and 0.08 ±0.01,respectively,superior to that of 3D-CRT(0.18 ± 0.02,t=7.80,7.65,P<0.05).The values of V10 for the heart of 3-and 5-field sIMRT were(8.4 ± 1.9)% and(8.4 ± 2.0)%,respectively,lower than that of 3D-CRT((36.0 ± 6.0)%(t=13.3,13.0,P<0.05).V20 for the thyroid gland were (12.4±1.5)% and(12.4±1.6)%,respectively,lower than(69.4±5.7)% of 3D-CRT(t=26.3,26.4,P<0.05).V20 for the larynx were(17.2 ± 1.2)% and(17.9 ± 1.5)%,respectively,lower than that of 3D-CRT[(89.4±7.0)%(t=25.5,26.5,P<0.05)].V30for the NTwas(4.4±1.4)%,(4.9± 1.9)%,lower than that of 3 D-CRT[(31.9 ± 6.1)%(t=8.5,10.1,P<0.05)].The averaged values of total MUs for 3-and 5-field sIMRT were 1100 ± 177 and 1160 ±204,respectively,higher than that of 3D-CRT(640 ± 151).Conclusions Compared with the 3D-CRT CSI technique,3-and 5-field sIMRT have better dose distribution and can improve target dose uniformity and protect the organs at risk.
7.Relationship between TSH and pregnancy outcomes during the first half of pregnancy in women
Fei ZHANG ; Wei PENG ; Yanlin LIN ; Yangang WANG ; Wenjuan ZHAO
Chinese Journal of Endocrinology and Metabolism 2017;33(1):52-55
[Summary] A total of 352 pregnant women were selected in Affiliated Hospital of Qingdao University. Serum levels of TSH and FT4 were determined and pregnancy outcome were observed in all subjects. According to the standard of American Thyroid Association(ATA) published in 2011 and the Chinese Guideline of Gestation Thyroid Disease published in 2012, the subjects were grouped into control(0. 1≤TSH≤2. 5 mIU/ L), observation(2. 5
8.Human placental mesenchymal stem cells promote angiogenesis
Jidong ZHAO ; Zongning MIAO ; Hanguang QIAN ; Wei PENG ; Zhiping SI
Chinese Journal of Tissue Engineering Research 2013;(23):4216-4223
10.3969/j.issn.2095-4344.2013.23.006
9.Lethal effects of nanoliposome encapsulated cisplatin on Saos-2 cells and its distribution in nude mice bearing human osteosarcoma xenografts
Guowen WANG ; Wei GUO ; Xiaodong TANG ; Changliang PENG ; Hui ZHAO
Journal of Peking University(Health Sciences) 2003;0(05):-
Objective:To investigate the killing effect of nanoliposome encapsulated cisplatin(NLE-CDDP) on human osteosarcoma cell line Saos-2 and explore the distribution of platinum(Pt) in tumor-bearing mice.Methods: Saos-2 cells were cultured at different concentrations of NLE-CDDP.MTT assay,inverted microscopic observation and flow cytometry assay(FCM)were used to observe the antiproli-ferative effect of NLE-CDDP on the human osteosarcoma cells.Antitumor effect of NLE-CDDP was determined using the xenografts models of human osteosarcoma cell Saos-2 in nude mice.The Pt concentration in the tissues of tumor-transplanted mice was determined by atomic spectrophotometer.Results: When treated at different concentrations of NLE-CDDP for 24-96 hours,the survival rate of Saos-2 cells decreased significantly(P
10.Expression of membrane inhibitor of reactive lysis in non-small cell lung carcinoma and its clinical significance
Weipeng ZHAO ; Bo ZHU ; Wei PENG ; Yuzhong DUAN ; Zhengtang CHEN
Chinese Journal of Primary Medicine and Pharmacy 2009;16(4):-
Objective To survey membrane inhibitor of reactive lysis(MIRL) expression in non-small cell lung careinoma(NSCLC) and to analyze the relationship between MIRL expression and clinical staging, adjuvant chemotherapy and disease-free survial. Methods The expression of MIRL in 8 adjacent tissues and 36 NSCLC sam-pies were determined by immunohistochemistry. Furthermore, the relationship between MIRL expression and clinical stage ,adjuvant chemotherapy and disease-free survival was assayed by follow-up. Results Among 36 samples of non-small-cell lung cancer,there were 10(27.8%) samples expressing MIRE. Out of 18 samples of squamous carcinoma, 4(22.2%) expressed MIRL,while 6(37.5%) expressed it in 16 samples of adenocarcinoma,there was no statistical significance between them(P>0.05). There were no expression in 2 samples of large cell carcinoma. There was no correlation between MIRL expression and disease-free survival(P>0.05). MIRL positive expression rate in patients with preoperational adjuvant chemotherapy was significantly lower than that of those without preoperational adjuvant chemotherapy(P<0.05). Conclusions There is great percentage of MIRE expression in NSCLC. Our present study suggests that the immunological inhibition of MIRL should be blocked when monoclonal antibody is used in the treat-merit of NSCLC.