Objective To explore clinical effect and safety of amiodarone combined with bisoprolol in patients with congestive heart failure and ventricular arrhythmia. Methods Selected 100 cases with congestive heart failure and ventricular arrhythmia in our hospital from January 2011 to April 2017 as research objectives and divided them into two groups randomly with 50 cases in each group. Provided amiodarone to control group and provided amiodarone combined with bisoprolol to observation group. Compared two groups' arrhythmia and cardiac function, heart rate, ejection fraction, QT time (QTc) corrected for heart rate before and after treatment as well as adverse events. Results Observation group's effective rate of arrhythmia treatment and the effective rate of cardiac function were 94.00％, 96.00％, those were significant higher than control group's 70.00％, 78.00％ (P<0.05). Two groups' heart rate and ejection fraction score after 3 month treatment were significant higher than those before treatment (P<0.05), and observation group's improvement was more significant (P<0.05). Observation group's QTc was significantly prolonged after 3 months of treatment (P<0.05), but control group's QTc did not change significantly. There was no significant difference in the incidence of adverse reactions between the two groups. Results Amiodarone combined with bisoprolol has significant clinical effect on patients with congestive heart failure and ventricular arrhythmia. It is safe and worthy to be promoted clinically.

OBJECTIVETo investigate whether bortezomib can enhance the sensitivity of human prostate cancer (PCa) cells to natural killer (NK) cell-mediated cytotoxicity, and whether it produces the same effect on different PCa cell lines.

METHODSWe treated androgen-dependent PCa LNCaP cells and androgen-independent PCa DU145 cells with bortezomib at the concentrations of 0, 5, 10, 15, 20 and 25 nmol/L for 24, 48 and 72 hours, and then detected the proliferation and apoptosis of the tumor cells by CCK-8 and Annexin V/PI, respectively.

RESULTSThe proliferation rates of the DU145 cells treated with 15, 20 and 25 nmol/L bortezomib were (82.79 +/-2.04)%, (73.59+/- 2.95)% and (74.16+/- 6. 16)% at 48 hours and (71.24+/- 5.30)%, (51.20+/- 2.91)% and (38.02+/- 2.67)% at 72 hours, and those of the LNCaP cells were (77.04+/- 7.74)% , (42.61 +/- 6.62)% and (23.85 +/-6.04)% at 48 hours and (36.45 +/-7.02)%, (14.94 +/-5.76)% and (11.65 +/-5. 87)% at 72 hours, both significantly inhibited as compared with the control group (P <0.05). At 24 hours, the apoptosis rates of the DU145 cells treated with 15, 20 and 25 nmol/L bortezomib were (14.41 +/- 1.32)% , (16.13 +/- 1.55)% and (14.48 +/- 1.42)% , and those of the LNCaP cells treated with 20 and 25 nmol/L bortezomib were (12.77 +/- 1.28)% and (14. 84 +/- 1.65)% , significantly higher than those of the control group (P <0.05) , and the DU145 cells showed an even higher sensitivity to bortezomib than the LNCaP cells. Bortezomib failed to sensitize these two cell lines to NK cell-mediated cytotoxicity in short-term assay, while long-term assay manifested that the apoptosis rates of DU145 and LNCaP cells after treated with 20 nmol/L bortezomib + NK cells were (41.83 +/- 5.06)% and (30.31 +/- 3.62)% , respectively, significantly higher

CONCLUSIONBortezomib enhances the sensitivity of than those after treated with either bortezomib or NK cells alone (P <0.05). PCa cells to NK cell-mediated cytotoxicity and adds to the effect of current cancer therapies, and it is more efficacious for androgen-independent prostate cancer.

Apoptosis ; drug effects ; Boronic Acids ; pharmacology ; Bortezomib ; Cell Line, Tumor ; Cytotoxicity, Immunologic ; drug effects ; Humans ; Killer Cells, Natural ; drug effects ; Male ; Prostatic Neoplasms ; pathology ; Pyrazines ; pharmacology

Objective To investigate the relationship between ambulatory arterial stiffness index (AASI) derived from blood pressure monitoring and early signs of renal damage in patients with primary hypertension． Methods 74 primary hypertensive outpatients were divided into two groups according to their AASI values: normal AASI group (AASI≤0.51, n=40) and high AASI group (AASI>0.51, n=32). The urinary micro-albumin, glomerular filtration rates (GFR) were measured and compared. The relationship between AASI and micro-albumin, GFR were tested with Pearson correlation and multiple Logistic regression. Results Compared with those in the normal AASI group, the patients in high AASI group showed a higher level of urinary microalbumin (P<0.05) and a reduction in GFR (P<0.01). AASI was positively correlated with urinary microalbumin （r=0.32, P<0.001）, and negatively correlated with GFR （r=0.44, P<0.001）. After adjusting the potentially confounding variables, the odd ratio (OR) of AASI to renal damage was 2.18 （P=0.008，95%CI：1.76～4.34）. Conclusion The increase of AASI is associated with early signs of renal damage in patients with primary hypertension.

Diagnostic Techniques and Procedures ; standards ; Humans ; Infant, Newborn ; Jaundice, Neonatal ; diagnosis ; Reference Standards

Objective: To observe the effects of Zhichuanxiaoke strong solution on the immunity and irritability of mice. Methods: The carbon clearance index and the content of serum hemolysin of mouse were determined by colorimetry. The survival time of mouse was measured through hypoxia tolerance test under ordinary pressure and the anti fatigue swiming test. Results: Zhichuanxiaoke Strong Solution could obviously increase the carbon clearance index and the content of serum hemolysin, enhance the anti hypoxia ability and prolong the swiming time of mouse. Conclusion: Zhichuanxiaoke Strong Solution can increase physical immunity and the ability of auti irritability.

Purpose:To investigate the variant expression of human belomerase reverse transcriptase (hTERT) and NF ?B p65 protein and their relationships in gastric carcinogenesis.Methods:A total of 41 primary gastric cancers, 15 cases dysplasia, 23 cases intestinal metaplasia and 10 cases normal gastric mucosa were taken into this study. Expression of hTERTmRNT, hTERT protein and p65 protein were determined by in situ hybridization and immunohistochemistry. Results:①Among normal mucosa intestinal metaplasia dysplasia carcinoma,p65 expression were 0?34.18%(8/23)?53.33%(8/15)?60.98%(25/41),hTERTmRNA 10.00%(1/10)?39.13%(9.23)?66.67%(10/15)?85.37%(35/41) and hTERT protein 0?30.43%(7/23)?60.00%(9/15)?78 05%(32/41),respectively. All the three parameters were significantly increased in dysplasia and carcinoma, compared to normal mucosa, while the expression level were also significantly higher in carcinoma than in intestinal metaplasia ( P

This article elucidates the relationship between the human susceptibility to obesity and gene polymorphisms such as peroxisome proliferators-activated receptors(PPARs)and PPAR?coactivator-1,along with milestones in the formation and development of capacity for fat deposition during evolutionary history of human.An biological evolutionary analysis,identifying factors favoring the energy stores,may be helpful to the development of preventive public health strategies.

Objective:To investigate the expression of estrogen receptor ? and ?(ER? and ER?) in temporomandibular joints(TMJs) of Sprague-Dawley rats.Methods:The expression of ER? and ER? in TMJs was examined by SABC technique of immunocytochemistry, meanwhile the co-expression of them was detected by double-staining technique of immunocytochemistry.Results:①Intense ER? and ER? immunoreactivity was localized in the hypertrophic layer of condyle cartilage,and some immunoreactivity was found in osteocytes of mandible and temporal bone. ②The immunoreactivity of ER? and ER? was found in both nuclei and cytoplasms. Most of immunoreactivity of ER? was localized in nuclei, while ER? was distrubuted more evenly. ③The expression of ER? was wider than that of ER?.Conclusions:TMJ is one of target organs of estrogen.The expression of ER? is different from that of ER?,which suggests there may be different mechanisms directed by ERs.