Objective To establish a method for determination of chlorogenic acid, galuteolin and 3,5-O-dicaffeoyl quinic acid in chrysanthemums by quantitative analysis of multi-components by single marker ( QAMS ) . Methods Using chlorogenic acid as the internal standard substance, the relative correction factors ( RCFs ) of galuteolin and 3, 5-O-dicaffeoyl quinic acid were determined by HPLC.The contents of chlorogenic acid, galuteolin and 3,5-dicaffeoyl quinic acid in 12 samples of chrysanthemums were authentically determined by the external standard method, so as to to verify the accuracy of the QAMS method. Results The average relative error of contents of galuteolin and 3,5-O-dicaffeoyl quinic acid was 2.75% and 1.33%by the external standard method and QAMS, respectively. Conclusion The established RCFs have good reproducibility.In case of lack of standard substances, the QAMS method with chlorogenic acid as an internal standard for determination of 3 indicators in chrysanthemum is feasible.

Objective: To investigate the effect of silencing PI3K by small interfering RNA (siRNA) on the expression of FOXO3a in human breast cancer MCF-7 cells and the growth of xenografted breast carcinoma in nude mice. Methods: Plasmids of siRNA for PI3K gene (Psilencer1.0-U6-PI3K) were constructed and xenografted tumor model was established in nude mice by subcutaneous inoculation of MCF-7 cells. Twenty-seven nude mice with xenografted tumor were divided randomly into 3 groups. Group 1 was intratumorally administered by Psilencer1.0-U6-PI3K plasmid. Group 2 and group 3 were intratumorally given Psilencer10-U6 plasmid and physiological saline (group 1), respectively. The tumor volume and survival time of nude mice were recorded. The expressions of PI3K and FOXO3a was measured by Western blotting. Results: The tumor size was significantly decreased and the average survival time was significantly enlongated in Psilencer1.0-U6-PI3K-treated group (P <0.01). Western blotting analysis showed that the protein expression of PI3K in xenografted tumor was markedly decreased and FOXO3a was significantly elevated compared with control groups (P <0.05). Conclusion: Psilencer1.0-U6-PI3K markedly silenced the expression of PI3K gene, up-regulated the expression of tumor suppressor gene FOXO3a, inhibited the growth of xenografted tumor, finally prolonged the survival time of nude mice bearing human breast carcinoma.

Based on the lethal rate, positive and negative mutation rate, the optimal doses of UV, HNO2, UV + AO treating S. hygroscopicus NND-52-29 were determined. The mutant strain A19 that was obtained showed the derepres-sive property to glucose and the nigericin yield of A19 could be increased by 128%. When it was subcultured combined with natural screening, its stability was rather satisfied.

Objective To analyse the necessity and feasiblity about the diagnosis and classification of fibrocystic disease of breast, through using synthesize imaging methods.to increase the diseases diagnosis rate. Methods Using imaging checks (molybdenum photography,ultrasonography and infra - red scanner) for 600 breast's cases and contrast to pathogenesis if they were done operations. Results 143 cases were normal. 166 cases got other disease of breast,291 cases were fibrocystic disease of breast(48. 1%), 155 cases of them were performed operations and pathogenesis. the diagnosis similar rate between performed operations check and pathogenesis was 83. 2%. Conclusion The sensitivity of three kinds imaging check method is different each other,but one method can give some help each other. The synthesize imaging check can increase the diagnosis rate and avoid mis - diagnosis and doing needless operations.

Objective To analyse the necessity and feasiblity about the diagnosis and classification of fibrocystic disease of breast,through using synthesize imaging methods , to increase the disease ＇s diagnosis rate. Methods Using imaging checks (molybdenum photography,ultrasonography and infra-red scanner) for 600 breast＇s cases and contrast to pathogenesis if they were done operations. Results 143cases were normal,166 cases got other disease of breast,291 cases were fibrocystic disease of breast(48.1%) ,155 cases of them were performed operations and pathogenesis. the diagnosis similar rate between performed operations check and pathogcnesis was 83.2%. Conclusion The sensitivity of three kinds imaging check method is different each other,but one method can give some help each other. The synthesize imaging check can increase the diagnosis rate and avoid mis-diagnosis and doing needless operations.

BACKGROUND:The patients with neurogenic urinary incontinence could be cured to restore their urinary storage function through the implant of the suburethral sling made of appropriate materials in a reasonable way. Meanwhile the choice of materials and the safe and effective control of tension are crucial factors. OBJECTIVE:To review the implanted methods, effectiveness and safety of sling made of different materials in the surgical treatment of neurogenic stress urinary incontinence. METHODS:The PubMed database, English database, CNKI database and related English books were retrieved with computer from January 1986 to January 2012 for the articles on the urethral sling material, urethral sling placement methods, efficacy and safety. The key words were“urethral sling, neurogenic, urinary incontinence”in English and Chinese. Final y, 47 articles were included for review according to the inclusion and exclusion criteria. RESULTS AND CONCLUSION:Urethral sling is a safe, handy, and micro-invasive operation, by using autologous fascia or various synthetic materials to implant into the body. The mechanism of neurogenic urinary incontinence is mainly related with the change of bladder function or the insufficiency of urethral sphincter function. However, it is stil a big problem for us to assure the postoperative ability of such patients to store and control urine because of their complicated conditions.

Objective To evaluate the clinical efficacy and security of retroperitoneal laparoscopic ureterolithotomy(RLUL) for impacted ureteral calculi after radical cystectomy and ileal conduit.Methods 5 patients with unilateral impacted ureteral calculi after radical cystectomy and ileal conduit received RLUL were selected,and a retrospective study was performed for manner,duration of surgery,complications and length of stay and other indicators,and the treatment effect was evaluated.Results All procedures were successful and the mean operation time was (82.0±27.7)min,the extubation time was (5.4±1.1)d,hospitalization time was (10.0±2.9)d.Postoperative follow up for 6-48 months,there were no obvious complications.Conclusion The RLUL showed satisfactory availability and security for management ureteral calculi after radical cystectomy and ileal conduit.

Objective: To observe the clinical efficacy of acupuncture plus rehabilitation training in treating post-stroke deglutition disorders of qi-deficiency blood stasis pattern. Methods: Sixty-six patients with post-stroke deglutition disorders of qi-deficiency blood stasis patter were divided into an observation group and a rehabilitation group using the random number table method. The two groups both received conventional medications and supportive treatment for stroke. In addition, the observation group received acupuncture plus rehabilitation training while the rehabilitation group only received the same rehabilitation training. The interventions were conducted 3 times a week for a total of 4 weeks in both groups. They were evaluated using Kubota water swallowing test (KWST), Fujishima Ichiro food intake level scale (FILS) and symptoms score of traditional Chinese medicine (TCM) before and after treatment, and at the 1-month follow-up. The therapeutic efficacy was assessed at the 1-month follow-up. Results: The KWST grading and FILS result after treatment and at the follow-up were significantly different from those before treatment in both groups (all P<0.001); the results of these two items at the follow-up were not significantly different from those after treatment in the two groups (all P>0.05). There were significant differences in the KWST grading and FILS result between the two groups after treatment and at the follow-up (all P<0.05). The TCM symptoms score changed significantly after treatment and at the follow-up compared with that before treatment in both groups (all P<0.001). The TCM symptoms grading efficacy at the follow-up was significantly different from that after treatment in the observation group (P<0.05), while the difference was statistically insignificant in the rehabilitation group (P>0.05). The TCM symptoms grading efficacy in the observation group was significantly different from that in the rehabilitation group after treatment and at the follow-up (both P<0.05). Conclusion: Based on the conventional treatment for stroke, acupuncture plus rehabilitation training or use of rehabilitation training alone both can improve the clinical symptoms in post-stroke deglutition disorders of qi-deficiency blood stasis pattern, but acupuncture plus rehabilitation training can produce more significant efficacy and better long-term efficacy in improving TCM symptoms.

Objective:To prepare and purify siRNA targeting a proliferation-inducing ligand targeted(APRIL-siRNA),so as to provxde a basis for studying the role of APRIL in human pancreatic cancer.Methods:pET-22b-APRIL was constructed to express APRIL dsRNA of human pancreatic cancer cell line CFPAC-1 in E.coli and the product was purified by chromatography using CF-11 column.APRIL dsRNA was digested by RNaseⅢto prepare APRIL siRNA,then the reaction mixture was loaded onto a DEAE ion exchange chromatography to remove RNaseⅢfrom oligonucleotides,and size exclusion chromatography was used to purify 21 bp siRNA.The purified APRIL siRNA was used to transfect Chinese hamster ovary(CHO)cells and the expression of APRIL in CHO cells was observed under fluorescence microscope Results:APRIL dsRNA was successfully expressed in E.coli after IPTG induction and was purified by CF-11 column.dsRNA was hydrolyzed with RNaseⅢand was purified by DEAE ion exchange chromatography and size exclusion chromatography.15% nondenaturing PAGE and 12% SDS- PAGE confirmed that RNaseⅢwas removed from oligonucleotides and 21 bp siRNA was purified with size exclusion chromatography.It was also found that APRIL siRNA obviously depressed APRIL expression in CHO cells.Conclusion:We have successfully constructed APRIL siRNA targeting APRIL gene of CFPAC-1 cells with in vitro transcription,which provides a basis for knock-down of APRIL gene in CFPAC-1 cells.

Smad3 is a major transporter in the transforming growth factor β (TGF-β) signaling pathway.It is in charge of the transfer of TGF-β signal from the surface of the cell membrane into the nucleus.The TGF-β signal can be bound to the target gene in the nucleus and regulate its expression.Abnormalities in Smad3 expression level and functional status will lead to abnormal signal transduction,involving cell growth,proliferation,development,differentiation,migration,apoptosis and other basic life activities.This review focused on the differential expression of Smad3 in hepatocellular carcinoma (HCC)and the adjacent tissue.The character of Smad3 in HCC is outlined in three parts:Smad3 upstream signaling source,Smad3 self-assembly maturation and Smad3 downstream effects,which may provide a summary and reference for the follow-up study on Smad3.