2.Application of high resolution melting analysis in detecting IDH1 mutation in glioma patients
Haiyan ZHANG ; Jie WANG ; Xiaodong CHEN ; Wei ZHANG ; Jiang XIAN
Chinese Journal of Primary Medicine and Pharmacy 2013;20(22):3379-3380
Objective To evaluate the feasibility of detecting the mutation of IDH1 in glioma patients by high resolution melting(HRM) curve analysis.Methods The gene mutation of IDH1 was detected by HRM method in 9 surgical resection paraffin specimens of glioma,and the result was verified by gene sequencing.Results 6 cases of R132H(CGT > CAT) mutation and 1 case of R132C(CGT > TGT) mutation were found by HRM method.The resluts of direct gene sequencing were consistent with HRM method.Conclusion The HRM method in detecting IDH1 mutation is more efficient and convenient than direct sequencing.Moreover,it has advantages of low-cost and suitable for clinical test.
3.Effects of individual nostalgia on positive feelings and sadness of spouses of postoperative patients with esophageal cancer
Di QIN ; Xian CHEN ; Hong LU ; Wei HAN
Modern Clinical Nursing 2017;16(5):14-17
Objective To investigate the effect of individual nostalgia therapy on positive feelings and sadness of the spouses of postoperative patients with esophageal cancer. Method From June 2014 to June 2015, 60 hospitalized patients and their 60 spouses were set as control group. Routine health education and guidance were conducted once or two times a week for 7 weeks. Another 60 cases of hospitalized patients and their 60 spouses during July 2015 and June 2016 were set up as observation group. The patients were treated with the same nursing intervention as in the control group, and their spouses were subjected to individual nostalgia treatment once a week for 7 weeks. Before the intervention (postoperative day 2) and 7 weeks after the intervention, the positive aspects of caregiving (PAC) and the Margit-Meuser caregiver grief ineventary short form (MM-CGI-SF) were used to investigate their positive feelings and sadness. Result The score on spouse's positive feelings in the observation group after intervention was significantly higher than those of the control group and that of the observation before intervention (P < 0.01). Conclusion Individual nostalgia can effectively improve the feelings of the spouses of esophageal cancer patients and reduce their grief.
4.Significance of C-Reactive Protein Monitoring to Guide the Course of Treatment with Antibiotic in Neonatal Bactenal Infection
xiao-jian, ZHOU ; xian-wei, CHEN ; zhong-quan, LU
Journal of Applied Clinical Pediatrics 2004;0(08):-
0.05). There was only 2/109 cases (5.8%) need a second course of antibiotics because of likely infection and 102/109 cases (93.5%)need not any moor antibiotics. The mean period of antibiotic treatment in group Ⅰ, group Ⅱa and group Ⅱb were (1.2?0.5) days,(4.8?0.8) days and (9.3?1.8) days,respectively.There were significant differences(all P
5.Protective effects of imperatorin against cerebral ischemia/reperfusion-induced oxidative stress through Nrf2 signaling pathway in rats
HE WEI ; CHEN WEI-WEI ; HUANG XIAN-HUA ; ZHOU YU-MEI ; LIAO FANG
Chinese Journal of Pharmacology and Toxicology 2017;31(10):988-988
OBJECTIVE To investigates the effects of imperatorin on the oxidative stress in the cerebral cortex and hippocampus after focal cerebral ischemia/reperfusion injury. METHODS Transient focal cerebral ischemia/reperfusion model in male Sprague-Dawley rats was induced by 2 h middle cerebral artery occlusion followed by 24 h reperfusion. Imperatorin (1.25 and 2.5 mg·kg- 1) or vehicle were administered intraperitoneally at 1, 5 and 9 h after the onset of ischemia. At 24 h after reperfusion, the biomarkers of oxidative stress such as the levels of reactive oxygen species (ROS), lipid peroxidation products malondialdehyde (MDA), nitric oxide (NO) and total antioxidant capacity (T-AOC), the activities of inducible nitric oxide synthase (iNOS), superoxide dismutase (SOD) and catalase (CAT) in the cerebral cortex and hippocampus were observed. We also assessed the nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and the NAD(P)H-quinone oxidoreductase 1 (NQO-1) protein expression by Western blot. RESULTS As compared to vehicle-treated animals, imperatorin treatment significantly reduced the ROS, MDA, NO levels and iNOS activity, increased T-AOC and the activities of SOD and CAT. Furthermore, imperatorin treatment also significantly induced the nuclear translocation of Nrf2, enhanced the protein expression of HO-1 and NQO-1 in the cerebral cortex and hippocampus. CONCLUSION Our findings indicate that imperatorin can protect the brain against the excessive oxidative stress induced by cerebral ischemia/reperfusion through activation of Nrf2 signaling pathway.
6.Treatment of castration-resistant prostate cancer: Evidence-based clinical practice.
Wei WANG ; Rui-xian PENG ; Guo-wei SHI ; Shi-yao CHEN ; Qiang DING ; Jia-yang HE
National Journal of Andrology 2015;21(12):1121-1124
OBJECTIVETo introduce the framework of evidence-based practice with a case of castration-resistant prostate cancer (CRPC) as an example.
METHODSA clinical question was formulated according the clinical scenario. A systematic search was conducted for the published literature in the databases of PubMed, EMBASE, Cochrane Library, Clinical Trial Registries, and Web of Knowledge up to Dec 2014. The identified literature was reviewed for quality appraisal before the evidence was applied to clinical practice.
RESULTSThe treatment was effective and the patient achieved disease remission.
CONCLUSIONEvidence-based practice should be integrated with clinical scenario, current evidence, and patients' willingness, and follow a systematic framework.
Evidence-Based Medicine ; Humans ; Male ; Orchiectomy ; Prostatic Neoplasms, Castration-Resistant ; therapy
7.Expression and significance of erythropoietin in human gastric carcinoma on tissue microarry.
Chen JIANG ; Jian-xian YU ; Hua CHEN ; Hong-jun WEI ; Hai-yan MA ; Ping JI
Chinese Journal of Pathology 2006;35(9):559-560
Adenocarcinoma
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metabolism
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pathology
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Adenocarcinoma, Mucinous
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metabolism
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pathology
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Adult
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Aged
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Aged, 80 and over
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Carcinoma, Signet Ring Cell
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metabolism
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pathology
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Erythropoietin
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metabolism
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Humans
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Hypoxia-Inducible Factor 1, alpha Subunit
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metabolism
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Immunohistochemistry
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Lymphatic Metastasis
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Microvessels
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chemistry
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pathology
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Middle Aged
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Stomach
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chemistry
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pathology
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Stomach Neoplasms
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metabolism
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pathology
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Tissue Array Analysis
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methods
8.11 cases of treatment of acute food poisoning .
Su-hui SU ; Xian-min GUE ; Jiang-hua WEI ; Jie CHEN ; Dan CHEN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(3):235-236
Acute Disease
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Adolescent
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Adult
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Animals
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Child
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Child, Preschool
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Female
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Foodborne Diseases
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diagnosis
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etiology
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therapy
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Humans
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Male
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Meat
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poisoning
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Middle Aged
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Pesticides
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poisoning
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Swine
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Treatment Outcome
9.Study on Alcohol-induced Osteonecrosis of Femoral Head of Phlegm-dampness Constitution with CYP2C8 Gene Polymorphism
Haiping LIU ; Mingwang ZHOU ; Shenghua LI ; Wei CHEN ; Binglin YE ; Tiefeng GUO ; Xian CHEN
Chinese Journal of Information on Traditional Chinese Medicine 2017;24(1):23-27
Objective To study the relation between alcohol-induced osteonecrosis of femoral head (AIONFH) related with high morbidity TCM constitution type with CYP2C8 gene polymorphisms.Methods Totally 152 Han nationality NONFH cases from Feburary 2014 to September 2015 from outpatient and the inpatient departments in Gansu Province Hospital of TCM were collected. 50 AIONFH cases were set as medical case group; meanwhile, 45 healthy volunteers were enrolled as control group. Database for medical materials of all patients and volunteers was established. TCM distribution for AIONFH patients was determined. Solution DNA extraction kit was used to extract DNA, and detect the concentration and purity of DNA. The target gene was amplified by PCR and the target gene was amplified by gel electrophoresis. The length of the fragment was confirmed to conduct target gene sequencing. With the results of sequencing and gel electrophoresis, the relation of AIONFH with CYP2C8 gene polymorphism in AIONFH patients with phlegm-dampness syndrome and the control group.ResultsThe CYP2C8 gene loci rs17110453 gene polymorphism was not statistically significant between the two groups (χ2=0.253,P>0.05). There was no significant difference in allele between the two groups (χ2=0.077,P>0.05). The risk of disease in CC genotype was 1.37 times higher than the AA genotype (95%CI: 0.339-5.540), without statistical significance (P>0.05). There was no significant difference in genotype and allele distribution between AIONFH patients with phlegm-dampness and non-phlegm-dampness and the control group (P>0.05).Conclusion CYP2C8 gene loci rs17110453 gene polymorphism A/C mutation has no obvious relation with AIONFH risk. There is no clear relationship between CYP2C8 gene loci rs17110453 gene polymorphism with AIONFH.
10.Effects of disease diagnosis and operative procedure on grouping of diagnosis related groups(DRGs)
Hehong WEI ; Ming LU ; Jianjun JIAO ; Xian LI ; Jianling LI ; Yushen CHEN ; Jianming CHEN
Chinese Journal of Hospital Administration 2015;31(11):869-871
Objective To learn the impacts of major diagnosis, other diagnoses, major surgery and other surgeries on the grouping of DRGs, and to optimize the DRGs data grouping quality by analyzing the main influencing factors of DRGs.Methods Based on regrouping results of the 1 940 questionable cases which have been corrected, using SPSS 13.0 to study the impacts of the questions found on the grouping of DRGs.Results 438 Patient records affecting DRGs grouping were regrouped according to major diagnosis, other diagnoses, major surgery and other surgeries.Influences of the above four groups on the grouping vary in general For comparison between two groups, P>0.007 1 between main surgery and other surgeries;while P<0.007 1 for comparison between other groups.Conclusion The highest influence of grouping was found in questionable major diagnosis, much higher than such other factors as other diagnoses, major surgery and other surgeries.This conclusion conforms to the steps of basic DRGs grouping logic-sorting the eases according to major diagnosis first of all Correct naming of surgery influences DRGs grouping, but the influence extent of major surgery and other surgeries is close.