Liver fibrosis can be classified as a wound-healing response to a variety of chronic stimuli,and is characterized by an excessive deposition of extracellular matrix(ECM)proteins.Hepatic stellate cells(HSCs)are presently regarded as one of the key cell types involved in the progression of liver fibrosis.Following a fibrogenic stimulus,HSC changes from a quiescent to an activated,collagen-producing cell.Each cellular response to extracelluar stimuli must be framed in a scenario.Along these lines,the identification and characterization of intracellular signaling pathways activated by different stimuli in HSCs represent a mandatory step.Drug targets which aim at the extra-cellular signals or intra-cellular cascades are required for ameliorating liver fibrosis.

Hepatic fibrosis is a pathological process in which excessive deposition of extracellular matrix components, occurs due to an imbalance between the production and degradation of matrix. Fibrosis is the hallmark of most chronic liver diseases. It is also the major factor of the development of chronic hepatitis and cirrhosis. Therefore, in light of the regulative factors on different levels and mechanism of fiber synthesis and degradation, antifibrotic medicine can inhibit the synthesis of matrix or increase its degradation on different links. The present situation concerning the study of the therapeutic effect of traditional Chinese medicine and natural medicine on hepatic fibrosis is reviewed.

Based on the "Grayscales average distribution" method which equally distributes the input gray levels to output gray levels, three improved methods named: "Reduce the gray range expressed by the less significant subfields", "Low levels preset" and "Modify the exponent of inverse-gamma function" are proposed in this paper. Using these methods, the inverse-gamma relation subfields code can be obtained easily which can improve the low level expressions of AC-PDP. And a program, "gray scales distribution validate program", which can enhance the expressions of the demanded gray levels range, is also proposed in this paper.

sham (P

This paper made some reflections on the development of traditional Chinese medicine(TCM) nephrology in recent years. It analyzed the strategic position and importance of social development in the field of prevention and treatment of diseases by TCM. Problems and puzzles on this subject had been summarized and countermeasures had been put forward. Preliminary conceptions on development strategies, goals and direction of TCM nephrology had been made.

Objective: To explore the relationship between CDR1 and CDR2 expression and fluconazole-resistance in clinical Candida albicans strains, so as to explore the mechanism of drug-resistance in Candida albicans. Methods: The minimal inhibitory concentrations (MICs) of clinical Candida albicans strains to fluconazole were determined by broth microdilution method. The total RNA of fluconazole-susceptible and fluconazole-resistant Candida albicans strains were extracted, and the expression of the CDR1 and CDR2 genes was examined by real-time RT-PCR. The efflux of Rhodamine 6G was determined in the strains highly expressing CDR1 and CDR2 genes. Results: The incidences of CDR1 and (or) CDR2 overexpression in fluconazole-resistant strains were significantly higher than those in the fluconazole-sensitive stains. The efflux of Rhodamine 6G was significantly increased in the fluconazole-resistant strains when glucose was added, and overexpression of CDR1 and CDR2 were observed in theses strains. Some resistant strains showed no overexpression of CDR1 and CDR2. Conclusion: Fluconazole-resistance in clinical Candida albicans strains is related to the overexpression of ABC transporter proteins. Overexpression of ABC transporters can increase the efflux of drugs and therefore lead to drug-resistance. Other mechanisms may also participate in the development of drug resistance in the clinical Candida albicans strains.

Objective To evaluate the effect of the bone grafting through a femoral neck window at high location in the treatment of osteonecrosis of the femoral head (ONFH).Methods From January 2009 to December 2011,26 patient (35 hips,11 males and 15 females) with ONFH were treated with bone grafting through fenestration at high level of femoral head and neck,and then they were followed up for average of 24 months,with the mean age of 37.4 years (range,18-65 years).According to the Association Research Circulation Osseous (ARCO) classification,there were 6 hips,13 hips,and 16 hips on stage Ⅱ A,Ⅱ B,Ⅱ C respectively.The patients were treated with the bone grafting through fenestration at high level of femoral head and neck.The fenestrated area was located in the junction of the femoral head and neck,involved part of the femoral head cartilage.The effectiveness was evaluated according to Harris Scoring system and excellent and good rate.Results All the cases were followed up in outpatient department for 24 months,and 1 was lost to follow-up.After the treatments with the bone grafting through fenestration at high level of femoral head and neck,no infections,nerve damage and other complications were found in all patients.Harris score of overall patients before and after treatment increased from 73.42±7.30 points to 85.85±11.63 points; Harris score of patients with ARCO Ⅱ A before and after treatment increased from 74.50± 1.76 points to 91.17± 1.60 points; Harris score of patients with ARCO Ⅱ B before and after treatment the average score increased from 73.92±8.03 points to 86.00±10.49 points.Harris score of patients with ARCO Ⅱ C before and after treatment increased from 72.60±8.29 points to 83.60± 14.29 points.The overall excellent and good rate before and after treatment in patients with preoperative 36.4％ up to 84.9％.The rate of patients with ARCO Ⅱ A increased from 33.3％ to 100.0％ after treatment.The rate of patients with ARCO Ⅱ B increased from 41.7％ to 91.7％ after treatment.The rate of patients with ARCO Ⅱ C increased from 33.3％ to 73.3％ after treatment.The difference in Harris scores and excellent and good rate between untreatment and treatment were statistical significant.Conclusion Bone grafting through a femoral neck window at high location in the treatment of osteonecrosis of the femoral head were proved to have good short-terms effects which can promote the restoration of osteonecrosis and improve the clinical symptoms of hip.

Unmethylated CpG motif containing oligodeoxynucleotide(CpG ODN) can induce secretion of dendritic cells,and thus presents antigens efficiently,increases expression of costimulatory molecules,causes innate immunity response and adaptive immunity response,then plays anti-tumor roles.

A Review] The neuroendocrine-immunoregulatory network is fundamental to the maintenance of homeostasis. In this article, the authors present an overview of the role of neuroimmunoregulatory mechanism in the occurance and development of some diseases, which may have important therapeutic implication for the clinics.