Adult ; Aged ; Body Mass Index ; Case-Control Studies ; Humans ; Male ; Middle Aged ; Neck ; anatomy & histology ; Sleep Apnea, Obstructive ; pathology ; physiopathology ; Young Adult

Objective To explore the feasibility and reliability of laparoscopic assisted thyroidectomy. Methods A transversal 2.0 cm incision was made at the site 1.0 cm above the sternal notch. By using a harmonic scalpel under a 5 mm laparoscope, the thyroid glands and involving blood vessels were dealt with by either “coagulation-dissection-suction” technique or “dissection-coagulation-disconnection” technique to complete thyroid enucleation or lobectomy. Results There were 20 cases of adenoma (nodule) excision and 6 cases of unilateral lobectomy. The operation time was 80~130 min (mean, 100 min). The intraoperative blood loss was 10~40 ml (mean, 20 ml). No surgical complications occurred. Follow-up checkups in 23 cases for 1~)20 months (mean, 9 months) found a satisfactory cosmetic result and no recurrence. Conclusions Thyroidectomy under laparoscope is safe and reliable. This procedure offers a shorter incision, less invasion and better cosmetic results as compared with conventional thyroidectomy.

Objective To explore the safety and invasion of endoscopic thyroidectomy(ET).Methods A total of 156 cases of benign thyroid diseases were surgically treated from January 2003 to December 2005,including 64 cases of endoscopic thyroidectomy(Endoscopic Group) and 92 cases of traditional thyroidectomy(Traditional Group).The operation time,hemorrhage volume,length of incision,Visual Analogue Scale(VAS) scores for pain severity and cosmetic appearance,analgesic requirements,postoperative hospital stay,hospitalization costs,wound infection rate,and recurrent rate were compared between the two groups,respectively.Results The operation was successfully completed in both of groups.No hemorrhage,hoarseness,or hypocalcemia was encountered.Wound hydrops occurred in 4 cases in the Traditional Group.As compared with the Traditional Group,the Endoscopic Group had less blood loss(15?10 ml vs 20?15 ml;t=-2.330,P=0.021),lower VAS pain scores(2.5?1.0 vs(5.0?)1.6;t=-11.079,P=0.000),higher VAS cosmetic scores(8.0?1.5 vs 2.0?1.2;t=27.696,P=0.000),and less analgesic requirements(10 cases vs 28 cases;?~2=4.493,P=0.034),but longer operation time(80?20 min vs 70?30 min;t=2.330,P=0.021) and higher hospitalization costs(5 352.7?978 yuan vs 4 738.5?672 yuan;t=4.651,P=0.000).Follow-up observations for 2~30 months(mean,13 months) revealed recurrence in 2 cases in the Endoscopic Group and 3 cases in the Traditional Group.Conclusions As compared with traditional operation,endoscopic thyroidectomy has advantages of minimal invasion,early recovery,short hospitalization time,and high safety,being an ideal option for benign thyroid diseases.

Adenocarcinoma, Clear Cell ; pathology ; Adult ; Female ; Humans ; Thyroid Neoplasms ; pathology

Objective To observe the immunologic effect of lactoferrin on repeated respiratory infection(IRRI) in children.Methods Ninety-eight cases of IRRI were divided into two groups randomly.The control group (48 cases)were treated with routine therapy.The treatment group(50 cases) were treated with lactoferrin based on routine therapy for 2-3 months.T cell subgroup,immunoglobulin and complements were determined before and after treatment.Results Total effective rates in treatment group and control group were 86% and 22.9% respectively.The therapeutic efficacy in treatment group was significantly higher than that in control group(P

Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; Female ; Hemopneumothorax ; surgery ; Humans ; Infant ; Male ; Middle Aged ; Paracentesis ; Thoracoscopy

OBJECTIVETo investigate the reversing effect of NF-kappaB inhibitor MG-132 on chemoresistance of gastric cancer cells to vincristine.

METHODSIn vincristine-resistant human gastric cancer cells (SGC7901/VCR) and the parental sensitive clone (SGC7901), NF-kappaB-DNA binding activity was determined by electrophoreses mobility shift assay (EMSA). The inhibition level of kappaB (IkappaB-alpha) expression was measured by cellular-ELISA. Immunocytochemistry was used to detect the translocation of p65 and chemosensitivity of the cells was determined by MTT assay.

RESULTSCompared with the parental SGC7901 cells, both the baseline and VCR-induced NF-kappaB-DNA binding activities in various concentrations were all higher in the SGC7901/VCR cells. Pretreatment with MG-132, the NF-kappaB inhibitor, for 30 minutes remarkably reduced the NF-kappaB activation, IkappaB-alpha degradation and nuclear translocation of p65. As to the SGC7901/VCR cells and the parental sensitive SGC7901 cells, the IC(50) values for VCR were 40.03 mg/L and 0.26 mg/L, respectively. MG-132 (2.5 micromol/L) significantly enhanced the toxicity of VCR in SGC7901/VCR cells and decreased the resistance index from 154.0 to 16.5. However, MG-132 did not show an obvious effect on the VCR sensitivity in sensitive SGC7901 cells.

CONCLUSIONOur data indicate that inhibition of NF-kappaB activation in gastric cancer cells may reverse the drug resistance to VCR in the cancer cells and increase the efficiency of chemotherapy.

Adenocarcinoma ; pathology ; Antineoplastic Agents, Phytogenic ; pharmacology ; Cell Line, Tumor ; Drug Resistance, Neoplasm ; drug effects ; Humans ; Leupeptins ; pharmacology ; NF-kappa B ; antagonists & inhibitors ; Stomach Neoplasms ; pathology ; Vincristine ; pharmacology

Objective To explore the protective effect of rosiglitazone on insulin resistance( IR)induced by high glucose in vascular endothelial cells and its possible mechanism. Methods Human umbilical vein endothelial cells( HUVECs) was divided into 3 groups:the normal control group cultivated in DEME medium with 5. 5 mmol·L-1 glucose;the high glucose group( HG)cultivated in DEME medium with 33 mmol · L-1 glucose for 24 h after the IR model was set up;the rosiglitazone group cultivated in DEME medium with 33 mmol·L-1 glucose and 10 μmol·L-1 of rosiglitazone for 24 h after the IR model was set up. The cell viability,nitric oxide(NO),endothelin-1(ET-1),mitochondrial membrane potential,reactive oxygen species ( ROS),p-IKK and IkBa protein levels were detected. Results Compared with the normal control,the cell viability,the level of NO and the mitochondrial membrane potential were decreased,levels of ET-1 and ROS increased,p-IKK expression was up-regulated,and IκBα expression was down-regulated in HG group(all P〈0. 01). Rosiglitazone reversed these changes in a time-dependent manner(P〈0. 05). Conclusion Rosiglitazone has the protective effect on insulin resistance induced by high glucose in vascular endothelial cells via inhibiting ROS/IKK signaling pathway.

Objective To characterize the spinal motion of 6 degrees of freedom (6DOF) under the fulcrum bending in Lenke type 1 adolescent idiopathic scoliosis (AIS) patients using 3D image volume fusion technique.Methods Ten volunteers with Lenke type 1 AIS were examined by the Gemstone Spectral Imaging to obtain their T1-S1 vertebral morphological data in the supine position and fulcrum bending position.With the computer software,images of 2 different positions were fused with the 3D image to obtain in vivo kinematics data of each vertebral body in the target area.Results In the apical vertebrae (AV) region,the main range of motion in the fulcrum bending position ranged from-2.57°± 0.95° to-1.37°± 0.65°,showing significant differences between segments (P ＜ 0.05);along the vertical axis the coupling rotation ranged from-2.71° ± 0.48° to-1.27° ± 0.49°;along the coronal axis,the coupling flexion and extension ranged from 0.19° ± 0.85° to 1.51° ± 0.42°.In the upper end vertebrae (UEV) area,the main ROM ranged from-2.20° ± 1.13° to-1.91° ± 0.76°,showing no significant differences between the 3 segments (P ＞ 0.05);along the vertical axis,coupling rotation ranged from 0.18°±0.47° to 1.35°±0.52°;along the coronal axis,coupling flexion and extension ranged from 1.62° ± 0.41° to 2.07° ± 0.37°.In the lower end vertebrae (LEV) region,the main movement ranged from-0.79°± 0.63° to 0.73°± 1.09°,showing significant differences between the 3 segments (P ＜ 0.05);along the vertical axis,the coupling rotation ranged from-1.62°± 1.05° to-0.60°± 0.60°;along the coronal axis,the coupling flexion extension ranged from -1.41° ± 0.87° to-0.75° ± 0.66°.Conclusionts In the fulcrum bending position,Lenke type 1 AIS shows a unique motion and coupling mechanism in the spine.The coupling rotational movement in the UEV region is at the same side as the lateral flexion movement.The coupling rotational motion in the AV and LEV regions is opposite the lateral flexion movement.

So far there exists no corresponding quality test procedures and grading standards for the seed of Prunus humilis, which is one of the important source of base of semen pruni. Therefor we set up test procedures that are adapt to characteristics of the P. humilis seed through the study of the test of sampling, seed purity, thousand-grain weight, seed moisture, seed viability and germination percentage. 50 cases of seed specimens of P. humilis tested. The related data were analyzed by cluster analysis. Through this research, the seed quality test procedure was developed, and the seed quality grading standard was formulated. The seed quality of each grade should meet the following requirements: for first grade seeds, germination percentage ≥ 68%, thousand-grain weight 383 g, purity ≥ 93%, seed moisture ≤ 5%; for second grade seeds, germination percentage ≥ 26%, thousand-grain weight ≥ 266 g, purity ≥ 73%, seed moisture ≤9%; for third grade seeds, germination percentage ≥ 10%, purity ≥ 50%, thousand-grain weight ≥ 08 g, seed moisture ≤ 13%.
Cluster Analysis ; Germination ; Prunus ; growth & development ; Seeds ; physiology