Objective To investigate the effects of different oxygen inhalation modes on retinal vessels development in neonatal rat in order to approach its etiopathogenesis and provide experimental evidence for proper oxygen therapy for premature.Methods Totally 150 postnatal day 7(P7)Wistar rats were randomly assigned into 5 groups according to different oxygen inhalation modes(n=30 in each group).Experimental group 1 were exposed to 75%,60%,50%,40% and 30% oxygen in turn for one day,followed by room air exposure for 5 d.Experimental group 2 were exposed to 30%,40%,50%,60% and 75% oxygen in turn for one day,then exposed in air for 5 d.Experimental group 3 were exposed to 75% oxygen for 5 d,then oxygen supply was suddenly stopped,followed by room air exposure for 5 d.Experimental group 4 were exposed to 75% oxygen for 5 d,then the oxygen supply was reduced by 10% per day till oxygen supply level equal to room air,finally room air exposure for 5 d.The control group were exposed to room air in the same experimental condition.The retinal vascular development and proliferation were evaluated by stretched preparation(ADPase stained retina)and eyeball cross-section.The nuclei of proliferative retinal vessels of 5 groups were counted and the vascular endothelial growth factor(VEGF)expressions in retina were detected by immunohistochemical staining.The dependability between the nuclei of proliferative retinal vessels and the VEGF expressions in retina were also analyzed.Results In the experimental groups 2 and 3,there existed retinal hypoxia and formed abnormal new vessels as retinopathy of prematurity(ROP).The VEGF expressions increased after hypoxia,mainly in endochylema and nucleus of capillary endothelium of ganglionic layer of retina and inner nuclear layer.Linear correlation analysis of the data indicated the positive correlation was between the nuclei of the proliferative vessels and the VEGF levels in retina(r=0.807,P

Objective To investigate the expression of Oct-4 as a stem cell marker in gastric carcinoma tissues and its clinical significance. Methods From June 1996 to March 2006, 63 paraffin samples of gastric carcinoma tissues were obtained, and the expression of Oct-4 was examined by immunohistochemical staining. Another 58 normal tissues adjacent to gastric carcinoma and 10 normal gastric mucosa tissues were served as controls. The clinieopathologieal data of 63 patients with gastric carcinoma were retrospectively analysed, and their relationship with the expression of Oct-4 was analysed. Fifty of these 63 patients were followed up for 8 years, Kaplan-Meier method and Log-rank teat were employed to explore the correlation between Oct-4 expression and survival, and Cox Regression analysis was performed to evaluate the possibility of Oct-4 expression as an independent prognostic factor for gastric carcinoma. Results The positive expression rate of Oct-4 in gastric carcinoma tissues was 80.95% (51/63), significantly higher than that of normal tissues adjacent to gastric carcinoma (5.2%, 3/58) and normal gastric mucosa tissues (0) (P < 0.01). The expression of Oct-4 was positively correlated to the clinical stage of gastric carcinoma(P<0.01). There was no significant difference in the expression of Oct-4 among patients with different differentiation, age, and gender (P>0.05). Patients with higher expression of Oct-4 had significantly shorter survival time (P<0.05). The expression of Oct-4 was not significantly correlated with the prognosis of gastric carcinoma(RR, 0.554; 95% CI, 0.209-1.466). Conclusion The expression of Oct-4 is correlated with the clinical stage and survival time of patients with gastric carcinoma, however, it is not an independent prognostic factor for gastric carcinoma.

Animals ; Brain ; metabolism ; Brain Ischemia ; blood ; enzymology ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Nitric Oxide ; blood ; Phytotherapy ; Rats ; Rats, Sprague-Dawley ; Superoxide Dismutase ; metabolism

Objective To investigate the the quasispecies character of 3′ untranlated region(3′ UTR) in hepatitis C virus(HCV) by analysing the nucleotide sequence polymorphism and mutation features in 3′ UTR region.Methods Patients infected with genotype 1b HCV were identified by reverse transcription-nested polymerase chain reaction(RT-PCR) and restriction fragment length polymorphism(RFLP) assay.Fragments of the cDNA of 3′ UTR were amplified using semi-nested RT-PCR,and subjected to cloning.The 12-15 clones that contained HCV 3′UTR gene fragments amplified from each patients were sequenced.Results The full-length sequence of 1b genotype HCV 3′UTR in cDNA were obtained.The 3′UTR region consists of four elements: the 5′ region,the poly(U),poly(U/C) and 98-base region.The nucleotide sequence diversity ranged from 0.2%～2.1% and the mutation points were almost distributed in the the 5′region and poly(U/C).Conclusions The HCV has complex quasispecies character in 3′ UTR.

BACKGROUND:Genotype of Kunming mice was similar to human population,thus an establishment of embryonic stem cell line is beneficial for research of transgenic animal.However,the best time to collect embryo has been less reported yet.OBJECTIVE:To find the best time to collect embryos from Kunming mice.METHODS:The embryos were collected from mother mice of 2.5,3.5,and 4.5 pregnant days.Microscope was used to evaluate the growth condition of embryos,embryo attaching rate(A/C),inner cell mass(ICM)growing rate(I/C),embryonic stem cells (ESCs)clone growing rate(P1/C)and ESCs subclone growing rate(P2/C).The cells were then stained with alkaline phosphatase.RESULTS AND CONCLUSION:Most of the 2.5-pregnant-day embryos were 16-cell-phase embryos.The 3.5-pregnant-day embryos were morulas while the 4.5-pregnant-day embryos were blastulas.There were no significant differences in A/C,I/C,P1/C,P2/C between 2.5 and 3.5 pregnant days(P ＞ 0.05).The 4.5-pregnant-day indicators mentioned above were significantly greater than those two groups;therefore,4.5-pregnant-day embryos were the best source to culture,clone,isolate and passage ESCs.

Objective:Breast cancer metastasis is a major cause of death. Lymphatic metastasis is one of the two main metastatic ways that is crucial to the metastasis process of breast cancer. To treat breast cancer metastasis, we prepared micelle-based drug carrier for lymphatic delivery. Methods:We used in vivo bioluminescence imaging for real-time dynamic monitoring of the inhibitory effect of polyethylene glycol phospholipid micelle encapsulating vinorelbine on breast cancer metastasis. Results: Compared with the free vinorelbine, vinorelbine-encapsulated micelles (NanoVin) showed a significantly enhanced anti-tumor activity both in primary and met-astatic tumors. Conclusion:Intravenous administration of NanoVin markedly prevented the metastasis of tumor cells through both he-matogenous and lymphatic systems. This study provides a new approach for treatment of metastatic tumors. Bioluminescence imaging is a powerful tool to dynamically monitor tumor metastasis and clinical therapeutic effect of anticancer drugs.

Child ; Diabetes Mellitus, Type 1 ; genetics ; Female ; Humans ; Infant ; Male ; Siblings

Matrix metalloproteinases (MMPs) are a family of Zn~ 2+ -dependent endopeptidases targeting extracellular matrix (ECM) compounds as well as a number of other proteins. Their proteolytic activity acts as an effector mechanism of tissue remodeling in physiologic and pathologic conditions, and as modulator of inflammation. Recently, it has been reported that MMPs play an important role in nervous diseases including cerebral ischemia, Alzheimers disease,multiple sclerosis and Parkinson′s disease.

Drug addiction is a chronic and relapsing brain disease, which causes damage to the health of addicts seriously, and causes huge social problems. Relapse is one of the major characteristics of drug addiction, and is the main problem to be solved. In the past several decades, the mechanisms, high relapse rates, abstinence of addiction were studied. The Results of these research findings are summarized in this review to provide a better overview of the mechanisms and treatments of addition in order to provide ideas for further research.

Objective To synthesize N-galactosylated chitosan as hepatocyte-targeting carrier and prepare loading norcantharidin nanoparticles.Methods N-Galactosylated chitosan was prepared by carbodiimide condensation reaction;loading norcantharidin nanoparticles were achieved by ionic cross-linkage process with N-galactosylated chitosan as carrier.Taking distribution of particle size,entrapment efficiency,and drug-loading capacity as comprehensive indexes,the orthogonal test design was used to optimize the preparation process and the in vitro release was investigated.Results Substitution degree of N-galactosylated chitosan reached to 8.92%.Novel nanoparticles were spherical,average in particle size(118.7?8.84)nm,entrament efficiency(57.92?0.40)%,drug-loading capacity(10.38?0.06)%,and the in vitro release followed Higuchi equation.Conclusion Effect of drug sustained release of galactosylated chitosan nanoparticles is significant.