1.LARGE-SCALE YEAST TWO-HYBRID SYSTEM IN THE APPLICATION ON MAP OF PROTEOME INTERACTOME
Hai-Rong MA ; Wei-Qi LI ;
Microbiology 1992;0(06):-
Protein fulfilling the their roles, one of important ways is through protein-protein interaction. In functional genomic era, identifying all of protein-protein interaction in proteome and mapping the protein interactions that have been attracting many scientists' attention , of which large-scale yeast two-hybrid system is one strategy of most widely used. In recent two years, ambitious projects have launched to examine all of the protein-protein interaction in Saccharomyces cer-evisiae using large-scale yeast two-hybrid system. Nevertheless, huge protein network is larger than that we predict and single yeast two-hybrid system cannot solve all the problems, which need be complemented by other wags.
2.Surgical Treatment and Clinical Characteristics of Children′s Thalamic Tumors
li-quan, XU ; wei, XU ; qi-wu, XU ; rong, XU
Journal of Applied Clinical Pediatrics 2004;0(11):-
Objective To study the clinical characteristics and effects of surgical treatment of children with thalamic tumors.Methods The clinical data of 22 cases were retrospectively studied and followed-up 6 months to 9 years.Results There were 13 boys and 9 girls,their ages ranging from 3 to 13 years.The average duration of symptoms before diagnosis about 2 months.Headache and papilledem were the most symptoms and signs,respectively.Most children′s thalamic tumors were low grade tumors with clear verge.In this group,good results were obtained that total remove 9 cases,subtotal remove 8 cases,partial remove 3 cases,biopsy 2 cases and no surgical death.Conclusions Clinical character of children thalamic tumors is distinct and good surgical results in the nearly future.The long results are determined by type of pathology.
3.Studies on gastrointestinal absorption enhancement effect and mechanism of sodium N-8-(2-hydroxybenzyl)amino caprylate to insulin solution.
Rong QI ; Qi-neng PING ; Wei ZHAO
Acta Pharmaceutica Sinica 2003;38(12):953-956
AIMTo study the enhancement effect and mechanism of sodium N-[8-(2-hydroxybenzyl) amino] caprylate (SNAC--a kind of synthetic enhancer) to insulin (INS) solution in gastrointestine.
METHODSTo determine the enhancement effect of SNAC on INS absorption by oral administration to rats and mice; To study the enhancement mechanism of SNAC by three kinds of methods: Delivering SNAC and INS solution to different parts of rats' intestines, adding energy inhibitor 2,4-dinitrophenol (DNP) or P-glycoprotein (P-gp) inhibitor verapamil (Ver) into SNAC and INS solution.
RESULTSSNAC was shown to enhance the gastrointestinal absorption of INS, the intensity of absorption enhancement corresponded to the doses of SNAC. The enhancement of SNAC to INS in different parts of the rat intestine was different (jejunum > colon > ileum). The effect of SNAC on INS absorption increased accordingly.
CONCLUSIONThe enhancement of SNAC to INS absorption presented dose dependence on SNAC; the absorption process needed energy and related to P-gp efflux.
2,4-Dinitrophenol ; pharmacology ; Animals ; Caprylates ; administration & dosage ; pharmacology ; Colon ; metabolism ; Dose-Response Relationship, Drug ; Ileum ; metabolism ; Insulin ; metabolism ; Intestinal Absorption ; drug effects ; Jejunum ; metabolism ; Male ; Mice ; Rats ; Rats, Sprague-Dawley ; Verapamil ; pharmacology
4.DNA aptamer selection in vitro for determining ketamine by FluMag-SELEX.
Mei-Qi SUN ; Fang-Qi CAO ; Xiao-Long HU ; Yu-Rong ZHANG ; Xin-Wei LU ; Li-Bo ZENG
Journal of Forensic Medicine 2014;30(5):346-349
OBJECTIVE:
To select specific DNA aptamer for determining ketamine by FluMag-SELEX.
METHODS:
Based on magnetic beads with tosyl surface modification as solid carrier and ketamine as target, a random ssDNA library with total length of 78 bp in vitro was compounded. After 13 rounds screening, DNA cloning and sequencing were done. Primary and secondary, structures were analyzed. The affinity, specificity and Kd values of selected aptamer were measured by monitoring the fluorescence intensity.
RESULTS:
Two ssDNA aptamers (Apt#4 and Apt#8) were successfully selected with high and specific abilities to bind ketamine as target with Kd value of 0.59 and 0.66 μmol/L. The prediction of secondary structure was main stem-loop and G-tetramer. The stem was the basis of stability of aptamer's structure. And loop and G-tetramer was the key of specific binding of ketamine.
CONCLUSION
FluMag-SELEX can greatly improve the selection efficiency of the aptamer, obtain the ketamine-binding DNA aptamer, and develop a new method for rapid detection of ketamine.
Aptamers, Nucleotide/metabolism*
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DNA
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DNA, Single-Stranded/genetics*
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In Vitro Techniques
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Ketamine/metabolism*
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Oligonucleotides
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SELEX Aptamer Technique/methods*
5.Cationic liposomes loaded with doxorubicin targeting to the tumor neovasculature in vitro.
Wei ZHAO ; Hui-Li MA ; Xian-Rong QI
Acta Pharmaceutica Sinica 2007;42(9):982-988
This study was conducted to investigate the in vitro characteristics of cationic liposomes composed of 3beta-[N-[2-(N', N'-dimethylamino) ethyl] carbamoyl] cholesterol (DC-Chol) and dipalmitoylphosphatidylcholine loaded with doxorubicin (DXR), and to provide useful information for the in vivo tumor vascular targeting of cationic liposomes. Cationic liposomes composed of different amounts of DC-Chol (0 mol%, 10 mol%, 25 mol%, 50 mol%) were loaded with the conventional anti-cancer drug doxorubicin. Their size, zeta potential, encapsulation efficiency, and DXR release in vitro were investigated. Moreover, their uptake by rat aortic endothelial cells (RAECs) was observed at 15 min, 30 min, 1 h, and 4 h of incubation. FITC-Dextran was i.v. injected to the H22 tumor-bearing KM mice to stain the neovasculature. The characteristics of resulting DXR-loaded cationic liposomes were in stable characteristics with particle sizes around 100 - 200 nm and capsulation efficiency greater than 90%. Increased cationic lipid led to enhanced zeta potential, and meanwhile it also resulted in quick release of the loaded drug, indicating increased slits or pores on the membrane upon the addition of DC-Chol. RAECs could more avidly take up DXR-loaded cationic liposomes when the content of DC-Chol increased in the liposomes, and DXR were quickly released in the cytoplasm and transported to the nuclei. The neovasculature stained by FITC-Dextran was clearly observed. DXR-cationic liposomes composed of DC-Chol could be used for tumor vascular targeting in vivo for further study.
Animals
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Aorta
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cytology
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Cell Line, Tumor
;
Cholesterol
;
analogs & derivatives
;
chemistry
;
pharmacokinetics
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Doxorubicin
;
administration & dosage
;
pharmacokinetics
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Drug Carriers
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Drug Delivery Systems
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Endothelial Cells
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metabolism
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Female
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Liposomes
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chemistry
;
pharmacokinetics
;
Liver Neoplasms, Experimental
;
pathology
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Male
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Mice
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Neovascularization, Pathologic
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metabolism
;
Particle Size
;
Rats
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Rats, Sprague-Dawley
6.Investigation of the plasma cortisol and the function of hypothalamic-pituitary-adrenal axis in type 2 diabetic patients
Lihua WANG ; Wei LIU ; Shengxian LI ; Rong HUANG ; Qi CHENG ; Yihua WU
Chinese Journal of Endocrinology and Metabolism 2008;24(5):525-526
The plasma level and the regulation of cortisol in type 2 diabetic patients were invesligated.Plasma and urinary cortisol levels were measured, and dexamethasonc suppression test and oral cortisone test in vivo were performed. Compared with controls, diabetic patients had higher urinary cortisol level. The activity of hepatic 11 β-hydroxy-steroid dehydrogenase-1 (11β-HSD1) in type 2 diabetic patients was decreased, suggesting that the elevated basal cortisol in type 2 diabetic patiens may due to impaired hepatic degradation of cortisol.
7.Effect of M2 macrophage against rejection on islet allografts in diabetic mice
Qi LIANG ; Ting LIU ; Li FENG ; Lingling DENG ; Pengfei RONG ; Wei WANG
Journal of Central South University(Medical Sciences) 2017;42(7):783-789
Objective:To explore the possibility of using peritoneal alternatively activated M2 macrophages to prevent rejection after islet allotransplantation in a murine model.Methods:Peritoneal monocytes from C57BL/6 mice were induced and modulated to M2 and M0 macrophages in vitro,then the phenotype of macrophage was assessed by flow cytometry.C57BL/6 mice were induced diabetic by streptozotocin (STZ) injection and transplanted with islets isolated from BALB/c mice under the left kidney capsule.The recipients were randomly divided to 3 groups (n=8).A total of 2.5× 106 M2 macrophages were injected intravenously at 0,3,7 d after transplantation in islet+M2 group;2.5×106 M0 macrophages were injected intravenously at 0,3,7 d after transplantation in islet+M0 group;the mice in islet+PBS group were injected with PBS.Blood glucose was monitored after transplantation.On day 10 after transplantation,2 recipients in each group were randomly selected and sacrificed,and the left kidneys were resected for pathological examination.Results:Achievement of euglycemia was significantly prolonged after islet transplantation in the islet+M2 group than that in the other two groups (P<0.01).The median survival time of islet allografts in the islet+PBS group,the islet+M0 group,and the islet+M2 group were 6.5 (4-10),7.5 (4-10),and 24(> 15) d,respectively.Pathological examination also showed that the grafts in islet+M2 group remained an intact structure with positive insulin stain and no apparent lymphocytes infiltration,while the graft was rejected in other 2 groups with negative insulin stain and massive lymphocytes infiltration.Conclusion:Peritoneal alternatively activated M2 macrophages can prevent rejection after islet allotransplantation,prolong the survival time of islet allografts and enhance the tolerance of the recipient to blood glucose in mice.
8.Atorvastatin inhibits the expression of ATP-binding cassette transporter A1 mRNA and protein in THP-1 macrophages induced by PMA
Xiuping WANG ; Chunli RONG ; Wei YU ; Ruyi LI ; Yingxin PENG ; Xiaoyong QI
Basic & Clinical Medicine 2006;0(03):-
Objective To investigate the effect of atorvastatin on expression of ABCA1(mRNA,protein) and LXR? mRNA in THP-1 macrophages induced by PMA.Methods Cultured THP-1 cells were induced to differentiate into macrophages by PMA for 48 hours.The macrophages were incubated with atorvastatin in different concentions for 24 or 48 hours.We determined the changes of ABCA1 mRNA,protein and LXR? mRNA by reverse trancriptase polymerase chain reaction(RT-PCR) and immuno-histochemistry.Results The expression level of ABCA1 mRNA(ratio of relative expression 1.21 vs 1.48) and protein as well as LXR? mRNA(0.87 vs1.12) were decreased in THP-1macrophages when cultured with atorvastatin(10 ?mol/L) for 24 h.Conclusion Atorvastatin inhibits the expression of ABCA1 mRNA and protein as well as LXR? mRNA of THP-1macrophages in vitro.
9.Effect evaluation analysis of three years intensive intervention for patients with type 2 diabetes at Xinjiekou Community Center in Beijing
Yuling LI ; Qi YUAN ; Aiwu TANG ; Shuo CHEN ; Wei SUN ; Rong KANG
Chinese Journal of General Practitioners 2013;12(11):899-901
Following the wishes of volunteer patients,316 cases of type 2 diabetics of our community center in 2008 carried out a three-year intervention from 2008 to 2011 on the basis of rational drug therapy plus the interventions of health education,regular review and lifestyle strengthening.And regular monitoring and recording were performed on the parameters of body mass index (BMI),blood pressure,blood lipids,fasting glucose,2 h postprandial blood glucose,glycosylated hemoglobin (HbA1c) and liver & kidney function.After intervention,some indicators changed:BMI (25.2 ±3.5) vs.(25.0 ±3.3) kg/m2,systolic blood pressure (129.1 ± 11.8) vs.(126.2 ±7.9) mm Hg(1 mm Hg=0.133 kPa),fasting glucose (7.80 ±2.81) vs.(7.25 ± 1.96) mmol/L,2 h postprandial blood glucose (11.04 ±4.60) vs.(9.83 ±3.60) mmol/L,HbA1c (7.39 ± 1.61) vs.(7.17 ± 1.65)%,total cholesterol (5.08 ±1.21) vs.(4.74 ± 1.35) mmol/L,low density lipoprotein cholesterol(LDL) (3.09 ± 0.87) vs.(2.85 ±0.83) mmol/L,high density lipoprotein cholesterol (HDL) (1.27 ± 0.33) vs.(1.41 ± 0.32) mmol/L,serum creatinine 65 vs.72 μmol/L,uric acid 300 vs.317 μmol/L.And the differences were statistically significant (P < 0.05).After intervention,blood pressure compliance rate increased from 72.5% to 88.0%,LDL-C compliance rate improved from 27.2% to 38.6%,HDL-C compliance rate of 54.9% increased to 66.9%.And the differences were statistically significant (P < 0.05).The pre-intervention combined compliance rate of 11.4% (n =36) rose to 17.7% (n =56).And there was significant difference (P =0.024).
10.Effect of motor impersistence on functional recovery and prognosis of patients with left hemiplegia
Wei CHEN ; Dong-Mei LIU ; Hai-Tao LU ; Qi ZHANG ; Tong ZHANG ; Shu-Rong JI ;
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(10):-
Objective To investigate the influence of motor impersistence on functional recovery and prog- nosis of patients with left hemiplegia. Methods A total of 64 in-patients with left side hemiplegia who were able to understand the oral instructions were assigned into a motor impersistence group (n = 31 ) and a control group (n = 34) , respectively, according to the assessment with Scale of Motor Impersistence developed by Joynt. Both groups were comparable in terms of the basic clinical characteristic and motor function of patients except motor impersis- tence. The conventional rehabilitation training programs were adopted for all patients in this study. Brunnstrom func- tional category, Ueda Satoshi hemiplegic finger function assessment scale, upper limb function assessment scale, Berg balance scale, Functional ambulatory category, Modified Barthel Index were employed to evaluate the subjects before and after 1 and 2 months of treatment, with regard to the motor function of their upper limbs, walking ability, activities of daily living performance and balance ability. Results After 1 month of treatment, the patients in the M1 group were significantly improved with regard to the motor function of upper limbs, hand function, standing ba- lance and activity of daily living performance( P0.05 ) , while those in the control group were improved with all the parameters except the lower limb function. After 2 months of treatment, patients in both groups were all significantly improved ( P