Objective To analyze the correlation between the severity of diabetic foot (DF) and the levels of serum UA,Hcy, HbAlc and LDL-c in Nanjing area, and to provide a basis for evaluating the severity of DF. Methods A total of 628 T2DM patients in Nanjing area from June 2019 to June 2020 were selected as subjects. According to whether patients had DF, they were divided into control group (n=502, without DF) and observation group (n=126,with DF). DF patients were divided into 6 grades (0-5) according to Wagner grading method. There were mild (0-1 grade, n=68), moderate (2-3 grade, n=42) and severe (4-5 grade, n=18). Serum UA, Hcy, HbAlc and LDL-c levels of diabetic foot patients in different severity groups were compared. ROC curve was drawn to analyze the efficacy of Hcy, HbAlc and LDL-C in the differential diagnosis of diabetic foot. Spearman correlation was used to analyze the correlation between the severity of diabetic foot and serum UA, Hcy, HbAlc and LDL-C levels. Results The serum levels of UA, Hcy, HbAlc and LDL-c in observation group were significantly higher than those in control group (P<0.05). The serum levels of UA, Hcy, HbAlc and LDL-C in severe group were significantly higher than those in mild and moderate groups ( P<0.05). The serum levels of UA, Hcy, HbAlc and LDL-c in moderate group were significantly higher than those in mild group (P<0.05). Spearman correlation analysis showed that DF illness severity and UA, Hcy, HbAlc, and there was a positive correlation of LDL-c (r=0.457 , 0.468 , 0.511 , 0.492 , P<0.05 ) ; ROC curve showed that the sensitivity and specificity of the combined detection of serum UA, Hcy, HbAlc and LDL-c in predicting the severity of DF patients were 95.65% and 96.78% respectively. Conclusion: Serum UA, Hcy, HbAlc and LDL-C of DF patients in Nanjing were significantly correlated with the severity of the disease, which has important clinical value for monitoring the progress of DF patients.