OBJECTIVE:To Study the antagonism of Huangshao granule on hepatic fibrosis in rats induced by CCl4.METHODS:60 rats were randomly assigned to blank group,model group,Huangshao granule groups(high,middle and low doses),Dahuang zhechong pill group.All the groups except the blank one were treated with CCl4 to induce hepatic fibrosis model.The primary end points were histopathological change of the liver tissues,activities of ALT and AST,content of albumin,levels of hyaluronic acid(HA),type Ⅲ precollagen(PCⅢ),type Ⅳ collagens(Ⅳ C)and laminins(LN).RESULTS:As compared with model group,in Huangshao granule groups(high and middle doses)and Dahuang zhechong pill group,the hepatic cellular structures showed better improvement,the activities of ALT and AST and serum levels of HA,PCⅢ,Ⅳ C and LN were decreased while the contents of albumin were increased.CONCLUSION:Huangshao granule showed satisfactory antagonism on hepatic fibrosis in rats,and further research and development of which remains to be carried out.

Developing and promoting college students' employment guidance work,setting up the right career ideal and professional concept,helping students make a reasonable career choice in the fierce competition have become the most prior work in the university. The essay mainly studies the present situation of college career guidance and the existing problems,discusses how to improve the employment guidance work in combination with the practice work in the new situation.

AlM: To analyze the effect of corneal limbus stem cell transplantation and amniotic membrane transplantation in treatment of recurrent pterygium with symblepharon.
METHODS:Totally 80 cases of patients with recurrent pterygium and symblepharon were randomly divided into pterygium resection combined with limbal stem cell transplantation group and pterygium excision with amniotic membrane transplantation group, 40 cases in each group. All patients were followed up for 6mo after the operation, the recurrence rate of pterygium and symblepharon were observed.
RESULTS:There were 2 cases of recurrence in the group of corneal limbus stem cell transplantation (5%), 8 cases of recurrence in the group of amniotic membrane transplantation (20%), with statistical difference between two groups (P<0. 05).
CONCLUSlON:Two kinds operation could significantly reduce the recurrence rate of recurrent pterygium, but the corneal limbus stem cell transplantation group has the lower recurrence rate.

Objective To investigate clinical features and the early recurrence factors of watershed infarction(WSI).Methods Two hundred and eighty-three patients with acute anterior circulation vascular infraction confirmed by CT or MRI were collected in Heilongjiang Province Hospital from January 2010 to December 2012.Patients' information including gender,sex,risk factors for stroke and vascular stenosis was colleced.Patients were divided into the lacunar infarction group (n =83),large infarction group(n =60) and the WSI group (n =140).All patients were followed up for 6 months to observe cerebral infarction recurrence status.The national institutes of health stroke scale(NIHSS) test of all patients was performed.Meanwhile the information including disease stage was collected and analysed.Results (1) The recurrent rate in WSI group,large infarction group and lacunar infarction group were 40.0％ (56/140),30.0％ (18/60),9.6％ (8/83)respectively.The difference between recurrent rate and lacunar infarction group was statistically significant(x2 =23.5,x2 =9.7,P ＜ 0.05),and the recurrent rate of WSI was highest.(2)The symptoms of patients with WSI were relatively mild in most patients after the initial stroke.75.7％ (106/140)WSI patients were 0-4 points regarding of NIHSS score,22.9％ (32/140) for 5-9 points and only 1.4％ (2/140) for more than 10 points.The clinical symptoms aggravated obviously in recurrent WSI patients.Of recurrent patients,28.6％ (16/56) were with high NIHSS score (score ≥ 10 points).(3)The difference between recurrent group and unrecurrent group in terms of unstable plaque,baseline systolic blood pressure,degree of stenosis ≥ 70％ was statistically significant(P ＜ 0.05).Conclusion The recurrent rate is higher in WSI group than other infarction type.The clinical symptoms are relatively mild in most of the WSI patients after the initial stroke,but the symptoms turn serious when stoke recurrent and the prognosis is poor.The unstable plaque,baseline systolic blood pressure,degree of stenosis ≥ 70％ may be the risk factors of stroke recurrence.

Objective To explore whether health examination save medical insurance fund or not. Methods To observe the sparing medical cost which 371 health examination people avoid repeated checking and shorten the length of stay during hospitalization from March 2006 to March 2007. To observe the effect of sparing medical cost because of regular health examination, early discovery diseases, pretreatment promptly and avoidance transforming to advanced diseases. Results Because of avoiding repeated checking and shortening the length of stay,everybody save the medical cost about 570 yuan,371 people can save about 21 ten thousands yuan. To statistics the average hospital cost of different courses of eight common diseases and malignant tumors from January to November at 2007, we discovered that the medical cost is large distinction between the earlier period and advanced stage of disease. For example: the medical cost of one stage of hypertension is 6608 yuan ,the two stage is 8838 yuan ,the three stage is 13 869 yuan, and the stage of hypertensive heart disease is 13 033 yuan. Parts of health examination crowd who stay at my hospital from 1996 were visited for five years ,we conformed many chronic diseases by prompt intervention can relieve and even reverse, avoiding the transformation to incurable disease which will consume a large number of strength and money. Conclusion Health examination resource is a medical treatment resource which isn't restricted by social security fund, the utilization can not only save medical cost but also discover and treat diseases earlier, thus we can save the strength and money before the advanced diseases.

Objective To study the preparation,quality control and clinical efficacy of compound of the nose Kang.Methods The compound of the nose Kang was extrasted through recirculation,qualitative identification and clinical efficacy of borneol,berberine and panax notoginseng in this compound was detected by thin layer chromatography(TLC).Results Preparation process is stable,thin-layer chromatography have obvious spots,clinical effect is significant.Conclusion The compound preparation is reasonable,simple process,quality is stable,effective,and has no side effects.

Humans ; Immunity ; drug effects ; physiology ; Infant ; Infant Nutritional Physiological Phenomena ; Nutritional Status ; Vitamin A ; immunology

Objective To evaluate the therapeutic effect and safety of insulin lispro mix 50/50 combined with mefformin in newly diagnosed overweight/obese type 2 diabetic mellitus patients.Methods Sixty-two patients with newly diagnosed overweight/obsess type 2 diabetic mellitus were randomly divided into observation group (32 cases) and control group (30 cases) by systematic sampling method.The observation group received insulin lispro mix 50/50 combined with metformin,and the control group received recombinant human insulin and insulin glargine.The therapeutic effect and safety were compared between the 2 groups.Results There were no statistical differences in the blood glucose before eating,before retiring and at mane primo 3:00 between the 2 groups (P ＞ 0.05).There were no statistical differences in the time of blood glucose standard and rate of hypoglycaemia between the 2 groups (P ＞ 0.05).Daily insulin dosage and costs in the observation group were lower than those in the control group [(0.6 ± 0.1) U/kg vs.(0.8 ± 0.1) U/kg and (15.8 ±2.1) yuan/d vs.(21.3 ±2.6) yuan/d],and there were statistical differences (P ＜0.05).Conclusion Insulin lispro mix 50/50 combined with mefformin provides a convenient,effective and safe therapy for newly diagnosed overweight/obese type 2 diabetic mellitus patients and high cost performance.

Objective:To study the influence of transfer payment on the equalization of medical and health services.Methods:Based on panel data of China's 23 provinces from 2000 to 2014.the medical and health services equalization indexes were measured and developed as the explained index.The explanatory variables such as transfer payment,per capita GDP and health care input were involved.The threshold value of time and transfer payment were applied to developed to conduct the panel threshold regression.Results:Transfer payment significantly promoted the equal distrihntion of health service resources,but the elastic declined if transfered more than 33 billion yuan as the threshold.Meanwhile,there was a threshold effect that the elastic of transfer payment to the equalization of China's medical and health service declined after NCMC in 2003.Conclusion:To further increase transfer payment,it needed to enlarge the new farmers and scope,optimize the equal distribution of health service resources.

Objective To explore whether or not the WS/T 405‐2012 reference intervals for blood cell analysis(WS/T 405‐2012) was suitable for population in this area and testing systems in this laboratory .Methods According to the requirements of documents WS/T 402‐2012 define and determine the reference intervals in clinical laboratory(WS/T 402‐2012 ) and WS/T 405‐2012 ,40 cases of healthy individuals ,including 20 male cases and 20 female cases ,were selected .The blood samples were detec‐ted by using the Sysmex XN‐1000 automatic blood cell analyzer which had met the requirements of performance assessment ,then reference intervals of blood cell analysis from W S/T 405‐2012 were verified .Results The detection results of all items of blood cell analysis involved in this validation met requirements of W S/T 402‐2012 .Conclusion The reference intervals of blood cell analysis from W S/T 405‐2012 could be applied for this laboratory .