OBJECTIVE:To establish the method for simultaneous determination of 9 common inorganic anions in Huanglian shangqing tablet. METHODS:The accelerated solvent extraction-ion chromatography was adopted. Inorganic anions were deter-mined by Ion Pac AS11-HC anion exchange column,protected by Ion Pac AG11-HC column and eluted by hydroxide potassium so-lution(gradient elution)at the flow rate of 1.2 mL/min. The column temperature was set at 30 ℃,elution time was 18 min,and sample volume was 25 mL. RESULTS:The linear ranges of fluorinion,formate ion,nitrite ion,bromide ion,nitrate ion,sulfate ion,oxalate ion and phosphate ion were 0.1-5 mg/L(r=0.9990-0.9999). The limits of quantitation were 0.020,0.078,0.030, 0.058,0.052,0.068,0.084,0.064,0.074 mg/L,and the limits of detection were 0.005,0.024,0.008,0.017,0.015,0.022, 0.026,0.020,0.021 mg/L,respectively. RSDs of precision,stability and repeatability were all lower than 4.0%;recoveries were 80.00%-125.08%(RSD ranged 0.97%-2.47%). CONCLUSIONS:The method is simple,precise,stable and repeatable,and can be used for 9 common inorganic anions in Huanglian shangqing tablet.

Objective To investigate the effect of triptolide on asthmatic airway remodeling and signal transducer and activator of transcription 6 (STAT6), acid neutrophil chemokines (eotaxin) impact. Methods The total of 30 mice with ovalbumin (OVA) model of asthma were randomly divided into three groups, control group, asthma group and triptolide group. After 24 hours of the last shot, lung tissue was stained Bronchial inflammatory cell infiltration was determined by using semi-quantitative method and calculate the proportion of goblet cells in airway epithelial cells. Hydroxyproline was determined by McMillan airway mucus score. The mRNA level and protein level of STAT6 and eotaxin in airway epithelium were determined by RT-PCR and immunohistochemistry. Results Compared with asthma group, peribronchial inflammatory cells infiltration of triptolide group were reduced, which mucus index is (1.31 ± 0.23) and hydroxyproline is (284 ± 13) μmg/100 mg. it had a significant in asthma group (P < 0.05). Besides, the protein level and mRNA level of STAT6 and eotaxin were significantly decreased (P < 0.05). Moreover, it was a positive correlation between STAT6 and eotaxin level in airway epithelial (r = 0.668, P < 0.05). Conclusion Triptolide can inhibit airway remodeling and might through the down regulation of STAT6 and eotaxin expression.

[Objective]To introduce a method to obtain Schwann cells from newly born SD rats massively and purely by immunomagnetic heads method.[Method]SD rats that had been born within 5 to 7 days were used. Their bilateral sciatic nerves were dissected under sterile condition. Under 16?microscope the nerve fascicles and the epineurium were carefully extracted in oder to get the nerve tract without impurity cell. Then the nerve tract was cut into small particle about 1 mm3.Two enzymes (0.25% trypsinase and 0.2% collagenaseⅠ)were used to digest the particle of sciatic nerve specimens twice. After using 20% fetal bovine serum to stop the process of digestion,centrifugate(1000r/min,5 min) and DF12 culture medium was added into the precipitation.Seven days later,Schwann cells were purified with immunomagnetic heads.During the whole process,the change of Schwann cells was observed under the inverted biological microscop and vital force of Schwann cells was evaluated.The growth curve of Schwann cell was drawn with MTT.The Schwann cells was identicated under immunofluorescent test and record the purity.[Result]The cell separated from the SD rat's sciatic nerve and cultured in incubator was affirmed as Schwann cells.Immunomagnetic heads can be used to purify the Schwann cells.The 96% vigor and 98% pure Schwann cell cultures were generated and passaged after two days.[Conclusion]This method can obtain massive purified normal schwann cells to satisfy the need of tissue-engineered bioartificial nerve graft.

[Objective] To investigate the combined effects of different neurotrophin couples of NGF,bFGF,BDNF on rat spinal cord neurons.[Method]Spinal cord neurons were obtained from SD rats born within 1d,and then were seeded in culture plates.Different factors and their couples were added in each chamber while DMEM/F12 served as controls,concentration of NGF,bFGF or BDNF were 50ng/ml.Phase contrast microscope observation was done.At the 3rd and 7th day after incubation,the cells were detected by ?-tubulin3 immunofluorescence and Hoechst staining.The length of neuritis was measured,and the numbers of neuron cells and nuclears were determined.At the 1st,3rd,5th,7th and 9th day MTT method was used,and the growth curve was made according to OD results.[Result] The length of axon and positive rate in the experimental groups were superior to those in the control group(P

Objective To determine the monokine levels of serum and synovial fluid (SF) in patients with JSpAs and estimate the correlation between monokines and inflammatory indices.Methods Serum and SF monokines (IL 1?,IL 1?,IL 6,IL 8,IL 12 and TNF ?) were measured by sandwich ELISA in patients with JSpAs ( n =28) and healthy controls ( n =10).The correlation between serum monokines and inflammatory indeces and between both serum and SF monokine levels was analysed.Results Elevated serum level of IL 6 was found in patients with JSpAs as compared to healthy controls ( P

Radiotherapy is one of the main means of clinical treatment for cancer, although the continuous updating and development of technology and equipment, adverse reactions and side effects can't be avoided yet.Improving the clinical efficacy of radiotherapy and reducing its adverse reactions are the currently facing subjects.A large number of clinical studies showed that using traditional Chinese medicines(TCM)in tumor radiotherapy had significant effects of improving and controlling the clinical symptoms, improving the life quality of cancer patients, improving the sensitization of radiotherapy, preventing the tumor metastasis and recurrence, as well as improving immune function and long-term survival, etc.Meanwhile, TCM also had significant roles in reducing the damage of radioactive inflammation, decreasing the bone marrow suppression to improve peripheral blood, and reducing the toxicity of digestive system, etc.The combination of radiotherapy and TCM provides a broader space for the comprehensive treatment of tumors.

Aged, 80 and over ; Angioplasty, Balloon, Coronary ; Compartment Syndromes ; complications ; therapy ; Female ; Humans ; Radial Artery

Adult ; Aged ; Female ; Fluorodeoxyglucose F18 ; Humans ; Male ; Middle Aged ; Nasopharyngeal Neoplasms ; diagnosis ; diagnostic imaging ; pathology ; Neoplasm Recurrence, Local ; diagnostic imaging ; Neoplasm, Residual ; diagnostic imaging ; Positron-Emission Tomography

OBJECTIVE Passing through the investigation of disposable syringes use condition, to determine amount of syringes used in the hospital and extent of their contact with blood and body fluids, and to assess their waste polluted condition. METHODS By our hospital computer charge system, from May 1, 2006 to Apr 30, 2007 The used syringes were calculated. RESULTS A total of 923 676 plastic disposable syringes were used; from them 6 801 (0.74%) were contaminated by blood, an other body fluids (urine, gastric secretion, cerebrospinal fluid, and wound drainage) or cells, 16 892 (1.83%) were by anticancer drugs and 160 461(17.37%) were with different injections or contrasts. 739 522(80.06%)were utilized for dilute medicine in i.v. drip. CONCLUSIONS Eighty percent of syringes waste is without pollution, it can be used to regard as recycled resources.

OBJECTIVE To explore the efficientcy of washing and sterilization methods for the endoscopic cell brush. METHODS The contaminated cell brush with the mixture of Bacillus subtilis var.niger spore and blood was washed by four methods:manually washing,multienzyme plus manually washing,ultrasonic cleaner washing,and multienzyme plus ultrasonic cleaner washing,and then dipped in glutaraldehyde for 5 h or 10 h,fumigated with glutaraldehyde for 5 h,and sterilized with 3M ethylene oxide box.The positive rates of occult blood test(OB test),the cleaning rates of B.subtilis var.niger spore and the sterilization eligibility ratio of four serilization methods were compared. RESULTS The washing method that multienzyme plus ultrasonic cleaner washing could clear effectively the contaminated cell brush that the positive rates of OB test was 0 and the cleaning rates of B.subtilis var.niger spore was 99.90%.The positive rates of OB test and the cleaning rates of B.subtilis var.niger spore of manually washing was 56.67% and 90.87%.Fumigated in 3M ethylene oxide box or dipped in glutaraldehyde for 10 h could kill all the spores,but dipped in glutaraldehyde for 5 h or fumigated with glutaraldehyde for 5 h could not. CONCLUSIONS The mixed contaminated endoscopic cell brush should be washed effectively by multienzyme+ ultrasonic cleaner washing,and should be sterilized by fumigated in ethylene oxide box or dipped in glutaraldehyde for 10 h.