Aim To investigate the effects of paeonol on lipopolysaccharide ( LPS) and adenosine 5′-triphos-phate ( ATP) induced NLRP3 inflammasome activation in primary rat microglia and the mechanisms responsi-ble for this anti-inflammatory effects. Methods Pri-mary rat microglia were identified immunohistochemi-cally using the cluster of differentiation 11 b ( CD11 b ) antibody. Proinflammatory cytokine IL-1β was deter-mined by ELISA. Western blot was performed to ob-serve the protein expression of NLRP3 , ASC and caspase-1 in cultured primary rat microglia. The level of intracellular reactive oxygen species ( ROS) was mo-nitored by using the fluorescent probe 2′, 7′-dichlo-rofluorescein diacetate ( DCFH-DA ) . Results LPS (0. 5 mg · L-1 )/ATP ( 5 mmol · L-1 ) significantly increased intracellular ROS level and IL-1β secretion and upregulated NLRP3 , ASC and caspase-1 protein expression in primary rat microglia. Paeonol signifi-cantly decreased intracellular ROS level and IL-1β se-cretion, and inhibited LPS/ATP induced overexpres-sion of NLRP3 , ASC and caspase-1 in cultured primary rat microglia. Conclusion Paeonol can inhibit LPS/ATP induced NLRP3 inflammasome activation in pri-mary rat microglia, and this inhibitory effect may be through the suppression of intracellular ROS.

Objective To observe the changes of combined therapy on disci intervertebrales and their surrounding tissues and thus to determine its therapeutic effects on lubar intervertebral disc protrusion with magnafic resonance imaging. Methods After combined therapy including physical agents, drugs, traction, manipulation and remedial exercises had been adopted to treat 40 iubax intervertebral disc protrusion patients for 30 days, morphologic changes of disci intervertebrales and their surrounding tissues of these patients were observed by magnetic resonance imaging. Results The effective rate of combined therapy on lubar intervertebral disc protrusion was 82.5%, there were no obvious changes of thickness, salient degree of disci intervertebrales, anteroposterior diameter, transverse diameter and latero-cryptae extent of vertebral canal after the treatment. Conclusion Though the combined therapy can not change the morphous of lobar intervertebral disc protrusion, it may efficiently relief the clinical symptoms of patients with lobar intervertebral disc protrusion.

The main teaching methods for medical students and residents are didactic teaching,rote learning and apprenticeship.Although these teaching methods can be good for imparting knowledge,they are obviously insufficient for the training of technical skills,team cooperation and complex decision making.Application of biomedical engineering simulation in the anesthesia teaching can partially compensate for the insufficiency.The progress of biomedical engineering simulation in anesthesia teaching is reviewed and the educational trend was explored.

Objective To evaluate a new suture coil in endoscopic submucosal dissection (ESD). Methods Data of 15 patients with early gastrointestinal cancer undergoing ESD were retrospectively ana?lyzed. The edge of the lesion was pulled up by the new coil?assisted suture for the submucosal layer to be full?y revealed, then the submucosa were dissected,and the wound was stitched by suture coil. The success rate and complications were evaluated. Results The lesions were successfully resected in 15 patients. By pull?up, the submucosal blood vessels were completely revealed. No intraoperative uncontrollable bleeding oc?curred. Small perforation occurred in one patient, and was well stitched by the suture coil. The pull?ups were successfully completed in all patients, and the operation time was 5?10 min. The intraoperative slippage of fixed titanium clips occurred in two cases. All the wounds were stitched. Minor bleeding happened in 1 pa?tient, and was controlled by hemostatic clip. Conclusion The new suture coil is easy to use,and can effec?tively prevent and deal with the occurrence of intraoperative and postoperative ESD?related complications.

Retrospective analyses were conducted for the clinical data of two cases with pseudoMeigs' syndrome at our hospital.Both had respiratory symptoms,such as cough and dyspnea.Radiological examinations revealed ascites,pleural effusion and pelvic mass.The definite pathological diagnosis was pelvic malignant tumor.After surgical tumor removal,ascites and pleural effusion disappeared without recurrence.Along with reviewed cases from PubMed in the last decade,a total of 49 cases had pseudoMeigs' syndrome.The age range was 11-73 years.Their clinical manifestations include dyspnea (78％),abdominal distension (69％),cough (14％),abdominal pain (12％),fatigue (10％),weight loss (6％) chest pain (4％),fever (4％),abdominal mass (4％),and oliguria (2％).CA125 was commonly elevated.The primary tumors in pelvic cavity accounted for 78％.And ovarian metastasis from colon cancer was one of the most common in metastatic tumor.

Antibodies, Antiphospholipid ; blood ; immunology ; Antiphospholipid Syndrome ; diagnosis ; etiology ; immunology ; Child, Preschool ; Diagnosis, Differential ; Humans ; Male ; Partial Thromboplastin Time

Adult ; Aged ; Female ; Femur ; injuries ; Humans ; Male ; Manipulation, Orthopedic ; methods ; Middle Aged ; Patellar Dislocation ; diagnostic imaging ; pathology ; physiopathology ; therapy ; Radiography

It has been well known that apoptosis induction and cell cycle arrest are typical biological effects observed in cancer cells after proteasome inhibition. TH2 is a new natural xanthone analogue isolated from the resin of Garcinia hurburyi tree. Here, the cell growth inhibition of TH2 on human hepatocellular carcinoma cell line (Bel-7402) was evaluated in vitro using SRB assay. The treatment of 10 ?mol/L TH2 reduced the surviving fraction from 86% (12 h) to 17.2% (48 h). To assess whether TH2 induce apoptosis, the appearance of sub-G1 peak, a specific fraction for apoptosis was detected by flow cytometry analysis. Progressive increase in the percentage of apoptotic population was observed in a dose-and time-dependent manner. Furthermore, a cleavage of poly (ADP-ribose) polymerase (PARP), a marker of early apoptosis, was observed clearly when the cells exposed to 10 ?mol/L of TH2 for 24 h by immunoblotting analysis. In vitro activities of 20 S proteasome purified from human erythrocytes on fluorogenic peptide substrates revealed that TH2 inhibited the trypsin-like, chymotrypsin-like and peptidylglutamyl peptide hydrolyzing activities in dose-dependent manner. Moreover, the turnover of tumor suppressor p53, a sign of deregulation of cell cycle progression and apoptosis induction by classical proteasome inhibitors, was disrupted in Bel-7402 cells. All these data indicate that TH2 had inhibitory effect on the proliferation of Bel-7402 cells and induction of apoptosis, which might be related to its inhibition of proteasome.

OBJECTIVE To investigate the possible contribution of OprD expression and metallo-?-lactamases(MBLs) in Pseudomonas aeruginosa clinical stains resistant to imipenem.METHODS Clinical strains resistant to imipenem were screened for MBLs production by a disk diffusion synergy test and subjected to PCR assays with primers specific for MBLs.Sequence analysis was provided to identify the prevalence of MBLs gene.Biochemical properties of MBLs were determined by ?-lactamase assays with crude preparations of ?-lactamases.Expression of OprD2 was determined by quantitative RT-PCR and Western blot analysis.RESULTS Among 128 imipenem resistant strains,7(5.4%) and 10(7.8%) isolates were positive for VIM-2 and IMP-1 genes,respectively.Crud extraction of ?-lactamases showed imipenem-hydrolyzing activity and could be inhibited by treatment with EDTA.In these imipenem-resistant clinical isolates,OprD2 protein was low-expressed in 10 isolates(7.8%) and normally expressed in 12 isolates(9.3%) but not expressed in 106 isolates(83.3%).However,17 isolates(13.3%) of MBLs producing strains were all lack of OprD2 expression.CONCLUSIONS Reduced or lack of OprD2 expression is the essential mechanisms for most imipenem-resistant clinical isolates of P.aeruginosa.blaVIM-2 And blaIMP-1 are prevalent in P.aeruginosa clinical resistant strains and may lead to nosocomial infection.

OBJECTIVETo explore the feasibility of the treatment of upper eyelid sulcus using the expanded polytetrafluoroethylene implanted in orbit.

METHODSFrom July 2009 to April 2011, a total of 16 patients with upper eyelid sulcus were treated for recreating youthful periorbital appearance. To correct this kind of clinical characteristic, expanded polytetrafluoroethylene with different size were used as the orbital implant to restore the orbital contents and reinforce the bony support. upper eyelid sulcus was reversed effectively.

RESULTSWith average 2.5 year follow up, upper eyelid sulcus of 16 cases was reversed effectively. No postoperative complication related to globe or affected normal physical function was found.

CONCLUSIONSThe technique of orbital implantation with Expanded Polytetrafluoroethylene is beneficial for the treatment of the upper eyelid sulcus.

Blepharoptosis ; surgery ; Eyelids ; surgery ; Feasibility Studies ; Humans ; Orbit ; Polytetrafluoroethylene ; therapeutic use ; Postoperative Complications