Purpose:To evaluate clinical utility of carbon dioxide digital subtraction angiography(CO_2-DSA).Materials anti Methods:CO_2-DSA was performed in 85 patients including hepatic.renal,splenic,and super mesenterie arteriography in 75 cases,abdominal artefiography and extremity arteriography in 3 cases,direct portography in 6 cases, hepatic venography in 1 case.Delivery method met the behavior of the gas combination with optimal imaging technique and postprocessing method were applied in order to obtain image accuracy. The images quality of CO_2-DSA was then compared with that of conventional contrast agent arteriograms.Results:CO_2-DSA provided good visualization of the large vascular trunks and their 1st,2nd.grade branches of the target vessels,which could be beneficial for guiding interventional procedures.It was inferior to the conventional DSA in visualization of small vessels, however,CO_2-DSA was superior to conventional DSA in the detection of arterioportal shunts (APS),tumor blood sinuses(including Cavernous hemangioma),retrograde visualization of portal vein.The side effects were minimal.Conclusion:CO_2-DSA could provide accurate, clinical useful veseular images.CO_2-DSA is believed to be a safe and effective alternative to iodine contrast agent.

0bjective To compare the curative effects of GnRH-a and mifepristone in treatment of endometriosis after laparoscopic operation.Methods 114 cases of patients were selected from March 2012 to December 2014 in our hospital with endometriosis after laparoscopic surgery,randomly divided into mifepristone group,GnRH-a group and untreated group,each group for 38 cases.mifepristone group were treated by oral mifepristone one week after operation,25 mg,qd,continuing to take 6 months;GnRH-a group,post operative 1 week received injection of GnRH-a (Tamiflu)3.75 mg,once every 4 weeks,6 times in a row;The untreated group,fails to drug treatment.To compare the symptoms,relapse rate pregnancy rate and serum hormones including FSH, LH and E2 levels.Results After treatment, the total effective rate of GnRH-a and mifepristone group is significantly higher than the untreated group(P<0.05),and the recurrence rate is lower than the untreated group(P <0.05),the pregnancy rate is higher than the untreated groups(P<0.05).The recurrence rate,the remission rate and the pregnancy rate of mifepristone group are lower than the untreated group,there was no statistical significance.Conclusion Mifepristone and GnRH-a can both,effectively reduce the recurrence rate after EMT laparoscopic surgery,can guarantee the pregnancy rate,reduce the adverse reaction.

0.05) .Macroscopic and microscopic observations of all target organs did not reveal any pathologic changes. Conclusion: Domestic-made CO_2 is a safe vascular contrast agent, but further study is necessary to assess the safety of injecting, CO_2 into the cerebral circulation.

Objective To evaluate diagnosis value of PP65 antigenaemia assay(AA) on cytomegalovirus(CMV) infection in febrile children.Method One hundred and twenty-eight febrile children with febrile duration between one to two weeks,their blood preparations and urine aliquots were screened,indirect IF staining(antigen PP65),ELISA(CMV-IgM) and light microscope(urinary cytomegalic inclusion).Three methods above mentioned were used in all patients.Result The positive rate of PP65 antigenaemia,CMV-IgM and urinary cytomegalic inclusion was 17.2%,8.6%,6.3%,respectively.The positive coincidence rate of PP65 antigen with CMV-IgM and PP65 antigen with urinary cytomegalic inclusion was 50%(11/22),36%(8/22),respectively.Nineteen childrens antigenaemia PP65 became negative with patient′s condition improved.Three children′s PP65 antigenaemia remained positive,when they were clinic cured.Two of them became negative after two weeks and one after three weeks.Conclusion Antigenaemia PP65 is effective in early diagnosis of active CMV infection,predicting patient′s condition and providing early intervention and treatment.

Cell-penetrating peptide (CPP) is a kind of small molecular peptide which can pass through a variety of cell membranes. It can carry bioactive macromolecules into cells. Due to lacking of tissue-selecting and targeting behavior, the application of CPP in the field of tumor treatment is limited. Activatable cell- penetrating peptide (ACPP) has brought the dawn to the application of CPP. This review mainly introduces the applications of ACPP in the targeting antitumor drug delivery which was designed based on the differences between tumor microenvironment and normal tissues as well as the exogenous physical stimulation.
Cell-Penetrating Peptides ; pharmacology ; Drug Delivery Systems ; Humans ; Neoplasms ; drug therapy ; Tumor Microenvironment

s in clinical education and medical education assessment/monitoring system.Authors hold that these factors will contribute to a growing quality momentum in its education and Success in refoms.

Objective To explore the relationship between serum Cystatin C (CysC) ,hypersensitive C-reactive protein (hs-CRP) level and the carotid intima-media thickness(IMT) in patients with type 2 diabetes mellitus(T2DM) .Methods 85 patients with T2DM were divided into two groups according to IM T level :Normal IM T group and IM T increased group .40 healthy people were chosen as control .The level of CysC and hs-CRP were measured and IMT of the carotid artery was determined by color Doppler ul-trasonography in 85 diabetics and 40 normal subjects .Results The CysC levels in IMT increased group was higher than normal IMT group and control group(P<0 .05) .The hs-CRP level in IMT increased group was higher than that in the normal IMT group and control group(P<0 .01) .The analysis of correlation indicated that the level of hs-CRP had positive correlation with IMT .And there was no association between serum CysC level and carotid IMT (r=0 .104 ,P>0 .05) .Conclusion There is no definite correla-tion between serum CysC level and carotid IMT in patients with type 2 diabetes mellitus .Serum CysC level is not predictive index in carotid intima-media thickness of T2DM .Inflammatory play an important role in early carotid atherosclerosis in patients with T2DM .

Cell-penetrating peptide (CPP) is a kind of small molecular peptide which can pass through a variety of cell membranes. It can carry bioactive macromolecules into cells. Due to lacking of tissue-selecting and targeting behavior, the application of CPP in the field of tumor treatment is limited. Activatable cell- penetrating peptide (ACPP) has brought the dawn to the application of CPP. This review mainly introduces the applications of ACPP in the targeting antitumor drug delivery which was designed based on the differences between tumor microenvironment and normal tissues as well as the exogenous physical stimulation.

Objective Recently, a series of studies demonstrated that activation of local renin angiotensin system (RAS) may be related to tissue fibrosis, but the role of RAS in hepatic fibrogenesis has not been evaluated. The present study was designed: (1) to assess the effect of angiotensin converting enzyme (ACE) inhibitor and angiotensin Ⅱ type 1 (AT1) receptor antagonist in preventing hepatic fibrosis induced by CCl 4 administration in rats; (2) to investigate whether or not there are expression of AT1 receptors on hepatic stellate cells. Methods Study was conducted in male Sprague Dawley rats. Except for control group, three treated groups, either enalapril (10 mg/kg), losartan (10 mg/kg) or a combination of enalapril and losartan were given to the fibrotic rats (daily gavage), and saline vehicle was given to the control rats. After 6 weeks, liver fibrosis was assessed directly by hepatic morphometric analysis, which have been considered the gold standard for the quantitative measurement of fibrosis. The expression of AT1 receptors and ? smooth muscle actin (? SMA) in liver tissue were detected by immunohistochemical techniques. Results Compared to the rats of control groups, either enalapril or losartan, or a combination of two drugs can limit the expansion of the interstitium ( P 0.05). Expression of AT1 receptors was found in abundance in hepatic fibrotic interstitium of fibrotic rats, whereas only limited in vasculature in rats of control group to a very slight degree. Conclusions The present results demonstrated that: (1) activation of RAS is related to hepatic fibrosis induced by CCl 4; (2) angiotensin converting enzyme inhibitor and AT1 blocker might slow the propression of hepatic fibrosis; (3) activated hepatic stellate cell expresses AT1 receptors.

Diffuse large B cell lymphoma (DLBCL) is one of the most common non-Hodgkin lymphoma (NHL).Since the appearance of rituximab,the most common first-line therapy is rituximab in combination with chemotherapy.About 70 percent of patients could achieve complete remission (CR) and be cured finally.But one third of patients could not reach CR after frontline treatment or relapse early,especially the elder patients or high risk ones.In the 56th American Society of Hematology (ASH) annual meeting,a plenty of treatments focusing on these patients brought encouraging results,which makes it possible to further improve the CR rate.The progresses in relapse/refractory,high risk and special site DLBCL will be summarized in this paper based on the reports in the 56th ASH annual meeting.