Adolescent ; Adult ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Cytarabine ; administration & dosage ; therapeutic use ; Female ; Humans ; Leukemia, Myeloid, Acute ; drug therapy ; Male ; Middle Aged ; Prognosis ; Treatment Outcome ; Young Adult

Air Pollutants, Occupational ; analysis ; Cyanamide ; analysis ; Spectrophotometry ; Workplace

Acute Disease ; Adult ; Female ; Herbicides ; poisoning ; Humans ; Male ; Middle Aged ; Paraquat ; poisoning ; Retrospective Studies ; Young Adult

Animals ; Cells, Cultured ; Female ; Granulosa Cells ; metabolism ; Pituitary Adenylate Cyclase-Activating Polypeptide ; genetics ; metabolism ; Platelet Activating Factor ; pharmacology ; RNA, Messenger ; genetics ; Rats ; Rats, Sprague-Dawley

Objective To evaluate prognostic value of electroencephalogram (EEG) in neonatal with hypoxic ischemic encephalopathy (HIE). Methods Sixty two infants with HIE was studied by doing physical examination, intelligence test, CT and EEG. Their clinical outcome was compared to their EEG in the first month after birth. Results Incidence of sequelae in normal or mild abnormal EEG and severe abnormal EEG were 3%, 29%, respectively. Among three infants of EEG cases, two dead and one developed cerebral palsy infant. Among four of burst suppression found in the EEGs, two cases dead, one developed cerebral palsy, one was normal. In five of hypoactive EEG, one dead, one developed cerebral palsy, and the other was with low IQ. Conlusion The prognosis is related to the background activity of EEG. The presence of a burst suppression EEG pattern and a hypoactive/flat EEG are negative prognostic criteria.

Objective:To analyse the clinical characteristics and to study the effects of thalidomide combined with tripterygium glycosides in the treatment of Behcet disease.Methods:50 cases of Behcet disease were analysed retrospectively.26 of them were treated by thalidomide at 35 mg combined with tripterygium glycosides at 10 mg 3 times a day.24 cases were treated by levamisole at 50 mg 3 times a day with 3-day-treatment and 11-day-interval.All the patients were treated for 2 months.Results:Middle-aged male patients with chronic onset were common,oral ulcer was the first common symptom.The disease could damage all organs,however the oral mucosa,skin,oculus,genitalia and joint were commonly involved.Reaction of needle puncture was highly specific in the diagnosis.The effective ratio of treatment was observed in 92% of the patients treated by the combination of thalidomide with tripterygium glycosides and in 67% of those by levamisole.Conclusion:The clinical manifestations of Behcet disease are complex,its diagnosis mainly depends on symptoms and signs,reaction of needle puncture is helpful in diagnosis.Thalidomide combined with tripterygium glycosides is effective in the treatment of the disease.

Objective To evaluate the application effect of UK manual moving & handling skills in the training of preventing occupational lower back pain among nursing professionals,and explore effective ways of training on preventing occupational lower back pain to nursing professionals.Methods 86 nursing students,94 nurses with 12 months' work experience,and 88 nurses with work experience over 5 years were randomized selected,and they each were divided into the experimental group and the control group.For 86 nursing students,43 students in each group; For 94 nurses with 12 months' work experience,47 nurses in each group; For 88 nurses with work experience over 5 years,44 nurses in each group.A routine taught training was given to both groups,while the experimental group received an extra 25 academic hours' structured OLBP prevention training.Comprehensive proposition was used to assess theoretic learning outcome of all participants,and practical skills of all participants were tested through institutional cases.Ressults There was significant statistical difference between scores of participants from two groups,and the scores of the experimental group in nurses with 12 months' work experience were different from the experimental groups of nursing students and nurses with work experience over 5 years.Conclusions Structured OLBP prevention training should lead occupational health prevention and health promotion concepts,apply numerous teaching approaches,emphasize the content of maintaining physical and psychiatric health and occupational protection management,set new-enrolled nurses as the main focused group of training.

AIM: The aim of the present study is to investigate changes of five biochemical markers of bone turnover during the formation of ovariectomized rat model. METHODS: Three-month-old female SD rats were divided randomly into ovariectomized (OVX) group, sham-operated (sham) group and control group. Five biochemical markers of bone turnover levels, including serum osteocalcin (OC), total alkaline phosphatase (ALP), bone-specific alkaline phosphatase (BALP), tartrate-resistant acid phosphatase (TRAP) and hydroxyproline (HYP) were measured before and at 1, 1.5, 2, 2.5, 3 and 4 months after surgery. The right proximal tibias of rats were excised at the same time point for histopathological observation. RESULTS: Serum OC, ALP, BALP, TRAP and HYP levels in OVX group were significantly higher than that in sham group. As regard to the time of peak level arrived, the sequence of changes was as follows: TRAP/HYP→OC→ALP/BALP. There was a significant positive correlation between five markers. The pathological changes of trabecular bone in OVX group were only observed 3 months after surgery. CONCLUSION: The results suggest that postmenopausal osteoporosis has a high bone turnover rate. During the formation of ovariectomized rat model, changes of bone resorption markers precede changes of bone formation markers. Serum OC, ALP, BALP, TRAP and HYP are sensitive to evaluate the bone loss in the earlier stage of postmenopausal osteoporosis.

Animals ; Apolipoproteins ; blood ; Cattle ; Colostrum ; Esterases ; blood ; Female ; Insulin-Like Growth Factor I ; Lipids ; blood ; Nephrotic Syndrome ; blood ; Pregnancy ; Rats ; Rats, Sprague-Dawley

To study the transport mechanisms of drugs for transplacental treatment of fetal tachyarrhythmia, MDCKII-BCRP and MDCKII cell models was used. MDCKII-BCRP and MDCKII cell monolayer model was used to investigate the bi-direction transport of sotalol, propranolol, propafenone, procainamide and flecainide. Drug concentrations were measured by HPLC-UV or chemiluminescence. The apparent permeability coefficient (P(app)), efflux rate (R(E)) and net efflux rate (R(net)) were calculated. Drugs with R(net) greater than 1.5 were further investigated using cellular accumulation experiments with or without a BCRP inhibitor. The R(net) of sotalol, propranolol, propafenone and procainamide were less than 1.5, while R(net) of flecainide with concentrations of 20 and 5 μmol x L(-1) were 1.6 and 1.9, respectively. The results showed that the transport of flecainide on MDCKII-BCRP cell monolayer could be mediated by BCRP; and the affinity increased when the concentration of flecainide decreased. Cellular accumulation experiments further suggested that accumulation of flecainide in MDCKII-BCRP cells was significantly lower than that in MDCKII cells in a concentration-dependent manner. BCRP inhibitor quercetin (50 μmol x L(-1)) significantly increased the accumulation of flecainide in MDCKII-BCRP cells (P < 0.05). Our preliminary data showed that flecainide but not sotalol, propranolol, propafenone or procainamide can be a substrate of BCRP. Thus the effect of flecainide may be affected by the BCRP in the maternal placental trophoblast membrane layer when treating fetal tachyarrhythmia.