OBJECTIVE: To explore the relationship between injury age and expressions of erythropoietin (EPO) and its receptor EPOR in the brain tissue of rats after cerebral injury. METHODS: Seventy-two rats were randomly divided into control group (36 rats) and cerebral injury group (36 rats). The rats were sacrificed at 1, 2, 4, 8, 12, 24 h after cerebral injury (6 rats at each time point) and the brain tissues were extracted. The expressions of mRNA and protein of EPO and EPOR at different time points were detected by real-time fluorescent quantitative PCR and Western bloting. RESULTS: The expressions of EPO and EPOR increased within 24 h after injury. The expressions of mRNA and protein of EPO were related to the injury age, and the correlations were 0.875, 0.911, respectively (P < 0.05). The expressions of mRNA and protein of EPOR were related to the injury age, and the correlation coefficients were 0.936, 0.905, respectively (P < 0.05). CONCLUSION The expressions of EPO and EPOR increase gradually in the early stage of the rat's cerebral injury, which are associated with the injury age and could be a useful value for estimating injury age.
Animals ; Brain/metabolism* ; Brain Injuries/pathology* ; Erythropoietin/metabolism* ; RNA, Messenger/metabolism* ; Random Allocation ; Rats ; Receptors, Erythropoietin/metabolism* ; Time Factors

OBJECTIVE To investigate the changes in and clinical significance of C-reactive protein(CRP) and endotoxin in patients who are firstly examined in fever clinic.METHODS To review and study the results of CRP and endotoxin from 81 patients(including 48 patients who were diagnosed as bacterial pneumonia and 33 patients who were diagnosed as a cold) in fever clinic of our hospital in 2005.RESULTS In 81 bacterial infection patients, the analysis indicated the levels of endotoxin between the pneumonia patients and the patients with a cold had significant difference.And the levels in the first group were significantly higher than in the latter one.The levels of CRP had no difference in two groups.CONCLUSIONS The result indicates plasma endotoxin detection helps to determine the early diagnosis of serious infection.It is also of significance to select antibacterial rationally and handle syndrome in time.In fever clinic,the levels of endotoxin may be a method to help diagnosis and treatment.CRP could be help to estimate the bacterial infection or virus infection,but it could not adjudge the seriousness in bacterial infection.

Objective To explore the relationship betw een injury age and expressions of erythropoietin (EPO ) and its receptor EPO R in the brain tissue of rats after cerebral injury. Methods Seventy-tw o rats w ere random ly divided into control group (36 rats) and cerebral injury group (36 rats). The rats w ere sac-rificed at 1, 2, 4, 8, 12, 24 h after cerebral injury (6 rats at each tim e point) and the brain tissues w ere extracted. The expressions of m RNA and protein of EPO and EPO R at different tim e points w ere de-tected by real-tim e fluorescent quantitative PC R and W estern bloting. Results The expressions of EPO and EPO R increased w ithin 24 h after injury. The expressions of m RNA and protein of EPO w ere relat-ed to the injury age, and the correlations w ere 0.875, 0.911, respectively (P<0.05). The expressions of m RNA and protein of EPO R w ere related to the injury age, and the correlation coefficients w ere 0.936, 0.905, respectively (P<0.05). Conclusion The expressions of EPO and EPO R increase gradually in the early stage of the rat’s cerebral injury, w hich are associated w ith the injury age and could be a useful value for estim ating injury age.

OBJECTIVE To study the best timing of tonsillectomy for peritonsillar abscess (PTA).METHODS The clinical data of 326 cases with PTA were reviewed.Among them,167 cases (group 1) underwent tonsillectomy in the period of abscesses,the other 159 cases (group 2) underwent elective tonsillectomy.RESULTS In group 1,the mean unilateral bleeding was (10?2.3)ml during operation,postoperative bleeding rate was 3.0%, mean operation time was (10?1.2)min and mean hospitalization period was (8?1.1)d.While in group 2,the mean unilateral bleeding was (21?2.1)ml during operation,postoperative bleeding rate was 6.9%,mean operation time was (20?1.5)min and mean hospitalization period was (16?1.3)d.There were significant differences between the two groups in the bleeding amount,operation time,postoperative bleeding rate and hospitalization period (P0.05). CONCLUSION For patients with good general conditions and no contradiction for operation, tonsillectomy implemented 3-4 days after drainage of abscess shows obvious advantages than elective operation.

Objective To observe the effects of repetitive transcranial magnetic stimulation (rTMS) on the expression of glial fibrillary acidic protein (GFAP) and OX-42 in hippocampus and dentate gyrus in rats.Methods The rats were treated with 1 Hz, 100 mT TMS 10 min once a day for 14 days, and then the expression of GFAP and OX-42 in hippocampus and dentate gyrus were investigated by ABC technique of immunohistochemistry.Results Compared with the control group, there were no significant difference in the expression of GFAP and OX-42 between the two groups.Conclusion rTMS using our parameters does not cause brain injury in rats.

ObjectiveTo study the effect of nursing quality of bull’s eye pattern on caring cancer patients with religious belief. Methods Three hundred and thirty-six cancer patients with religious beliefs were cared according to nursing quality of bull’s-eye pattern.All patients were evaluated by European organization for research and treatment of cancer quality of life questionnaire core 30(EORTC-QLQ-C30)before and after applications of nursing quality of bull’s-eye pattern and the results were compared.Result The scores of the dimensions of overall quality of life and function in EORTC-QLQ C30 questionnaire among those patients with implementation of nursing quality of bull’s eye patterns were higher than those before implementation of the pattern and the symptom score after the use of the pattern was statistically lower than that before its use in respect of dimensions of cancer symptoms(P<0.01). Conclusion The applications of nursing quality of bull’s-eye pattern on caring cancer patients with religious beliefs can improve the patients’ quality of life.

38 patients with dentition defect in our hospital for magnetic attachment dental treatment were analyzed. At the same time,34 cases of patients with dentition defects were ,being treated with conventional prosthodontics, selected to compare the stability of overdentures and masticatory function with magnetic attachment. The stability of overdentures and masticatory function after restored with magnetic attachment was significantly higher than before(P< 0. 01),while there was no difference after 4 weeks. Cementation language,comfort and appearance satisfaction after 4 weeks were significantly higher than before(P < 0. 05). The stability of overdentures,masticatory function, cementation language,comfort and appearance satisfaction after restored with magnetic attachment was significantly higher than conventional prosthodontics(P < 0. 05). It finally indicated that magnetic attachment had the advanta-ges of comfort,convenience and good effect. The stability of overdentures,masticatory function and application sat-isfaction was superior to conventional prosthodontics,and it had an application prospect.

Objective To assess the possible sequestration of remifentanil by the extracorporeal circuit.Methods Two types of extracorporeal circuits (ECCs) were used-Xijian type I (group A)and AFFINITY(R) NT(group B),while in control group (group C) a glass container was used.The ECC and glass container were filled with priming solution (6%hydroxyethyl starch 1000 ml + lactated Ringer's solution 500 ml).Remifentanil Wag then added to the priming solution with the final concentration of 100 ng/ml.The priming solution was circulated in the closed ECC or stirred the glass container.The concentration of remifentanil in the priming solution was determined at 2,5,10 and 15 min after addition of remifentanil.Results The remifentanil concentration in the priming solution decreased by 75.1%,57.9% and 18.3%at 2 min after addition of the drug in group A,B and C respectively as compared with the baseline value.The remifentanil absorption by ECC was significantly greater in group A than in group B.There was no significant difference in remifentanil concentration at the 4 time points in each group.Conclusion ECC can absorb remifentanil.The remifentanil absorption by Xijian type I is significantly greater than that by AFFINITY(R).

Objective To compare the body fluid count results detected by Sysmex XE-5000 automatic blood cell analyzer and manual method .Methods A total of 300 cases of body fluid specimens (including cerebrospinal fluid and fluid of serous cavity ) were analyzed .RBC ,WBC counting and classification were respectively detected by XE-5000 and manual method of improvement Neubauer counting plate .Results The fresh specimens without contain a large number of cell clusters ,which RBC counts(RBC-BF)(100-10 000)× 106/L ,and WBC counts(WBC-BF) (9-50)× 106/L ,showed there were a linear relationship between the in-strument method(r=0 .998 5 ,0 .986 3) .In the range ,there was no significant difference between XE-5000 and manual method(t=9 .96 ,P>0 .05) .Also in this range the results of instrument correlated with those of manual method(r= 0 .989 3 ,0 .971 7 , 0 .924 9) .For those specimens which contain a large number of cell clusters ,RBC-BF and WBC-BF were a badly linear relationship between the instrument method(r=0 .564 8 ,0 .456 1) .Conclusion Body fluid specimens which are fresh and do not contain a large number of cell clusters ,in the range of RBC-BF (100 -10 000) × 106/L ,WBC-BF (9 -50) × 106/L ,Sysmex XE-5000 automatic blood cell analyzer could ensure the results have good accuracy .

Objective To investigate the training effect and application development of the health industry standard of Nursing Grading, and to explore it'sclinical applicability. Methods An electronic questionnaire survey was conducted among nurses in 30 three general hospitals of Shandong Province from November 10 to December 10 in 2015. The questionnaire mainly included four parts: basic information, training status, application status and the clinical applicability. All the questionnaires were automatically submitted by the nurse′s cell phone or computer, 3386 valid questionnaires were recovered. Results The status of standard training:93.92%(3180/3386) nurses received a comprehensive training about the standard. The answer rates of 4 theoretical examination questions was 88.95%(3012/3386), 84.49%(2861/3386), 85.17%(2884/3386), 65.71%(2225/3386)respectively. 77.26%of the nurses needed to continue training. Application status: 97.67% (3307/3386) nurses in the hospital has begun to apply Nursing Grading standard, and 97.01% (3208/3307) and 97.76% (3231/3307) of the hospital revised the standard of care systems and work processes; 78.23% (2649/3386) nurses divide the level of care based on both the patient's condition and self-care ability presently. 74.45%(2521/3386) nurses did it as follows,the doctor determine the patient′s condition level, the nurse determine the level of patient′s self-care ability, then the nursing grading was determined according to the both. In the clinical applicability survey, the choice proportion of very agree and agreeofHelping nurses to determine the patient′s level of care and It is beneficial to improve the efficiency of nursing management was 86.30% (2922/3386) and 82.66%(2799/3386);The choice proportion of very agree and agree ofHelp nurses to improve the quality of nursing services, reflecting the value of the nursing work, to meet the needs of patients was 79.36% (2687/3386), 76.56% (2592/3386), 80.98% (2742/3386) respectively. Conclusions The hospital attaches great importance to the standard of Nursing Grading, but the training effect needs to be improved;standard has been widely used in Shandong Province Physician-nurse collaboration has become the decision-making main body of nursing grading;the standard application can not only help the nurses to do decision-making correctly and improve the efficiency. It also can help nurses to improve the quality of nursing service, and enhance patients′satisfaction. So it is very suitable for clinical practice.