Objective:To provide the scientific reference basis in order to establish a perfect cost controlled system for medical consumable. To analyze game factors, such as main resource, the price-off influence and price-off aspiration and so on, from different views, such as medical institutions, patients, manufacturers and other stakeholders and so on, based on game theory and situation of medical consumables management.Methods: To collect the management information of medical consumables cost from a tertiary referral center hospital in Fujian in recent 3 years, and adopt descriptive study to analyze the income situation of medical consumable, medical consumable proportion and medical consumable cost per capita.Results: The medical consumable proportions from 2013-2015 were 14.44%, 16.27% and 18.75%, respectively. Medical consumable cost per patient from 2013-2015 were 3547.95RMB, 4122.3 RMB and 5059.02RMB, respectively. All of these datum contained the drug information.Conclusion: It is obvious that the medical consumable proportions and medical consumable cost per patient were enhancement from 2013 to 2015. The necessity of medical consumable leaded to the direct influence on medical cost. On the other hand, the artificially high price and unreasonable implement of medical consumable are the urgent problems and must be resolved as soon as possible.

Objective:To provide scientific references for establishing a whole informatization management system of reagent used in science and research of hospital based on the root cause analysis (RCA). And to find existed problems and analyze main reasons from reagent management, material, time, financial, personnel and other factors through fishbone chart, and to provide relative management measurement of informatization.Methods: The reagent consumptions used in science and research of a tertiary referral center hospital in Fujian province in 2015 year were collected, and then the purchase amount and ratio of them were analyzed.Results: The purchase amount of reagent for science and research of this tertiary referral center hospital in Fujian province was more than 2.55 million RMB in 2015 year, and the ratio of national clinical key special projects was 23.32% and that of provincial clinical key special projects was 6.85%. The two items spent more purchase amount, and the type and ratio of reagent of various department was different depended on their own various developed situation.Conclusion: The informatization management and service mode of reagent used in science and research have very important role in the construction of soft power of hospital. In present, a series of disadvantages existed in various clinical and research department, for example, the use of reagents are scatter, involved professional span is larger and disperse, and accurate trace, dynamic tracking and flexible deploy machines are difficultly achieved. Therefore, how to promote comprehensive informatization management for relative resource, for optimizing structure configuration, for controlling cost and ensuring quality of reagent has became the most important task for informatization management of reagent used in science and research.

Adolescent ; Anemia, Aplastic ; complications ; therapy ; Anemia, Hemolytic ; chemically induced ; complications ; Antilymphocyte Serum ; adverse effects ; therapeutic use ; Female ; Humans

Objective To investigate the effects of selective cyclooxygenase-2(COX-2) inhibitor nimesulide on the proliferation of colon adenocarcinoma cells in vitro and the expression of matrix metalloproteinase-2(MMP-2).Methods The human colon cancer cell lines HT-29 and HCT-116 were employed in the study,grouped as nimesulide group,DMSO control group and blank control group.After treatment with nimesulide,the inhibitory effect of nimesulide on the proliferation of cancer cells was quantified by MTT assay,and the expression of MMP-2 in the cells was detected by quantitative zymography.Results Nimesulide inhibited the proliferation of HT-29 and HCT-116 cells in time and dose-dependent manners.The inhibitory effect on HT-29 cells was stronger than that on HCT-116 cells.Nimesulide down-regulated the MMP-2 expression in HT-29 cells,whereas the expression in HCT-116 cells remained unchanged.Conclusion Nimesulide can obviously inhibit the growth of colon cancer HT-29 cells with positive COX-2 protein,suggesting that nimesulide may down-regulate the expression of MMP-2 by inhibiting the activity of COX-2.

0.05). Compared with preoperation, serum LH levels were significantly higher after operation in both groups(P0.05). DSA and color Doppler ultrasound examination showed that autografts had good blood supply. Conclusion The modified testicular autotransplantation operation could improve the successful rate of transplantation, and had not obvious influence on testicular function. It can be further applied in homologous testicular transplantation experimental and clinical research.

Objective To analyze the role of dysphagia during the course of sleep apnea in patients who experienced a first-ever ischemic stroke.Methods According to the video-fluoroscopy,50 patients were divided into two groups:dysphagia and non-dysphagia groups.The effect of different sites and severity of infarction on the breathing disturbance was analyzed,and the possible role of dysphagia was discussed.Results Patients with dysphagia showed more number of OAIs at the acute phase(AHI,20.9;OAI,19.1)than that of non-dysphagic patients(AHI,9.5;OAI,8.6).Multiple correlation analysis found the extent of dysphagia had relationship with the AHI,especially OAI(AHI,P=0.018;OAI,P=0.024).Conclusion The number and type of obstructive apneic events may occur at the acute phase of a first-ever cerebral infarction in patients with pharyngeal muscle alterations secondary to the neurological lesion.

We present the binding ability of a new peptide (CMPQVMPMC-) with dental enamel after being evaluated in the present study. Under a standard procedure, the recovery of M13 filamentous phage was greatly enhanced by displaying the peptide in phage coat protein p III. Then the cyclic peptide was synthesized using a solid method. The effect of the cyclic peptide in vitro biomineralization was tested in a single-diffusion microtiter plate gel system. Absorbance at 405 nm of each sample was recorded for 24 h at every 6 h intervals. The relatively increased values of each sample were expressed as percentages relative to the blank group (100%). The cyclic peptide resulted in a concentration-dependent delayed nucleation. In addition, the overall values of peptide groups at the end of 24 h were lower than those in the control group but much higher than those in the BSA control group.
Dental Enamel ; chemistry ; Peptides ; chemical synthesis ; chemistry ; Protein Binding ; Tooth Calcification

Abstract?AIM:To evaluate the effect of combined glaucoma and cataract surgery on the function of eye surface and tear film.?METHODS:This clinical trial involved 75 patients ( 75 eyes ) with glaucoma complicated with cataract undergoing combined glaucoma and cataract surgery.All the eyes were divided into surgical group ( the eyes undergoing operation ) and control group ( the other eyes).The symptoms and signs of dry eye disease, Schirmer's I test, break -up time ( BUT ) , corneal fluorescein staining were tested and marked 1mo after operation.Comparison of the results was made between two groups.?RESULTS:The average intraocular pressure was 16.25± 0.46mmHg at 1mo after operation, compared with 45.29± 4.39mmHg at 3d before surgery, the difference was statistically significant ( P<0.05 ).Preoperative average visual acuity was 0.08±0.06, corrected visual acuity was<0.05 in 23 eyes,≥0.05 to<0.1 in 16 eyes,≥0.1 to<0.3 in 36 eyes.Postoperatively 1mo, corrected visual acuity:<0. 05 for 7 eyes (9%),≥0.05 to <0.1 for 11 eyes (15%), ≥0.1 to<0.3 for 38 eyes (51%), ≥0.3 to <0.5 for 11 eyes (15%) , ≥0.5 for 8 eyes (11%), postoperative visual acuity was 0.15 ±0.1, which was significantly higher than before surgeries ( P<0.05).The postoperative dry eye symptoms of surgical group was higher, compared with that of control group, and the differences of diagnosis of dry eye test indicators between the two groups were statistically significant(P<0.05).? CONCLUSION: Combined cataract and glaucoma surgery may affect postoperative ocular surface and tear film function, make the tear film stability damaged and lead to dry eye disease.

Objective:To compare the interaction of withdrawal behaviors and correlated indices of two morphine dependent models of mice.Method: Mice were given morphine with gradual increasing dose to establish two models of morphine dependence with different dosage sequence, i.e. model 1 for 7 days, model 2 for 5 days. Withdrawal syndrome was precipitated by naloxone and recorded. The weight of heart, liver, lung, kidneys, adrenal glands, testicles of the mice were compared.Result:Both methods (7 days way and 5 days way) successfully established morphine dependent models in mice. In withdrawal, the mice of 7 days model had more average times of jump and “wet-dog” shaking than the mice of 5 days model had, they had also more obvious upper eyelid dropping and anterior claw tremble. The weights of heart, kidneys, testicles of the mice of 7 days model were significantly lower than the counterparts of the mice of 5 days model. Conclusion:Both 7 days way and 5 days way can establish morphine dependent model of mice, the former has advantage of more indexes applicable while the later has advantage of less dose of morphine and short time needed.

Objective:To study the difference of c-fos expression of neurons in subareas of hippocampus of morphine dependent mice.Methods:Mice were given (sc) increasing doses of morphine to form morphine dependent models and withdrawal syndromes were precipitated by naloxone. The intensity of withdrawal syndromes was evaluted accoding to indices ,such as the number of jumping ,the weight loss,et al .The expression of Fos positive neurons in subareas of CA1, dentate gyrus and CA3 of hippocampus of morphine dependent group was observed by immunohistochemistry assay. Results:The number of Fos positive cells in subareas of CA1 and dentate gyrus of morphine dependent group was much higher than that of the normal control group(P