1.Expression and function of P2X purine receptors in the enteric nervous system
International Journal of Surgery 2011;38(1):37-40
P2X receptors are ligand gated ion channels, extracellular ATP is their natural extracellular ligand. When any of the subtypes of P2X receptors and extracellular ATP are combined, P2X channel opens, allowing cations (calcium, sodium, potassium, etc. ) pass through. Enteric nervous system is composed of the gut myenteric plexus and submucosal plexus, in both of which P2X receptors exist, mediating different effects under physiological and pathological conditions. In this paper, the enteric nervous system,P2X receptor distribution and related functions are reviewed in order to further explore the P2X receptor in the diagnosis and treatment of intestinal diseases.
2.Significance of arylsulfatase A gene detection for the diagnosis of metachromatic leukodystrophy
Juncheng ZHU ; Bing WEI ; Hua WANG
Chinese Journal of Applied Clinical Pediatrics 2015;30(24):1859-1862
Objective To detect genetic causes of seizures and developmental retardation in 60 patients with abnormal head magnetic resonance imaging(MRI) ,and to analyze the clinical manifestations and head MRI manifestations in carriers of arylsulfatase A (ARSA) gene mutation.Methods The blood samples of children and genomic DNA were collected.Sixty cases of children with suspected metachromatic leukodystrophy were tested (MLD) by using the second generation sequencing technology.The genotype and phenotype and head MRI findings were analyzed.Results Of the 60 cases of children, 15 cases with gene mutations.There were 7 kinds of ARSA gene mutations, and 3 of them, c.1178C > G, c.1055A > G and c.883 G > A were pathogenic.The others were single nucleotide polymorphism(SNP), which had no relationship with this disease.One of the patients carried only SNP, and 14 of them were carrying pathogenic mutation, c.1055A > G (53.33%) ,c.1178C > G (40.00%) were more common,and c.1055A > G mutation was in 8 cases, of which 5 cases were late-onset type.One case of the 3 patients who were late infantile type was carrying c.1178C > G mutation at the same time.All the eight cases had retardation.One case had hydrocephalus, and 5 cases had epilepsy.All of the 6 patients with c.1178C > G were late-infantile type, and had retardation, including 4 cases of epilepsy, c.883G > A mutation in 1 case,was late-infantile type,and the first symptom was binaural deafness and mental retardation.Three different types of mutations showed no significant difference in brain MRI.Conclusions There are 14 patients who were diagnosed as MLD.c.1178C > G and c.883G > A were late infantile type,and c.1055A >G was mostly late-onset type.The changes in head MRI caused by different types of ARSA gene mutations were of no significant differences in performance.
3.Laboratory management for biosafety of medical researches
Hua GU ; Wei ZHU ; Jingqing WENG
Chinese Journal of Medical Science Research Management 2012;25(2):88-89,95
Laboratory study is an important part of medical research.In the past few years,many significant biosafety incidents occurred in the medical field in and out of China.We here try to analyse the problem of biosafety management of laboratories,stating the importance of biosafety management in laboratory,and put forward countermeasures.
4.Association between atopy for Platanus Acerifolia pollen and HLA-DRB1 alleles
Ming QI ; Hua WEI ; Qin ZHU ; Aili WANG ; Hua XIONG
Chinese Journal of Clinical Laboratory Science 2006;0(06):-
Objective To develop a PCR-SSP method for detection of HLA-DRB1 alleles in the patients who were hypersensitive to Platanus Acerifolia pollen allergen,and to probe into the association between the atopic subjects to Platanus Acerifolia pollen allergen and HLA-DRB1 alleles.Methods DNA in whole blood was extracted by phenol-chloroform method.Eight pairs of specific primers for alleles were synthesized,and HLA-DRB1*0401,*0402,*0403,*0404,*0405,*0406,*0407,*0408 alleles in 20 atopic patients and 36 healthy individuals of Jiangsu Province with Han nationality were detected by PCR-SSP(polymerase chain reaction-sequence specific primer).Results By optimizing the experimental conditions PCR-SSP methods for detection of the 8 alleles were established and the distributing data of above-mentioned HLA DRB1 were obtained.The frequency of HLA DRB1*0405 and *0406 in the patients group was higher than that of in healthy controls group,while the frequency of HLA DRB1*0402 in the patients group was lower than that in controls.No significant deference for the other 5 alleles was found between the 2 groups.Conclusion HLA-DRB1*0406和*0405 seems to be the likely suspected candidate alleles responsible for susceptibility to Platanus Acerifolia pollen allergen in the atopic patients,while DRB1*0402 might be contribute to the related resistance to the allergen.
5.Percutaneous transluminal abdominal artery cutting balloon angioplasty treatment in two children with Takayasu's arteritis.
Wei-hua ZHU ; Song-ling FU ; Wei WANG
Chinese Journal of Pediatrics 2009;47(2):148-149
Abdominal Cavity
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blood supply
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Adolescent
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Angioplasty, Balloon
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Child
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Female
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Humans
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Male
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Takayasu Arteritis
;
therapy
6.Nasal septal perforation combined with chronic invasive fungal rhinosinusitis: three cases report.
Hua-ming ZHU ; Wei-tian ZHANG ; Yu-jun ZHANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2012;47(8):692-693
Aged
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Humans
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Male
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Middle Aged
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Mycoses
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complications
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Nasal Septal Perforation
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complications
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microbiology
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Sinusitis
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complications
;
microbiology
7.The expression research of osteopontin in nasopharyngeal carcinoma tissues
Shihai XUAN ; Yugui ZHOU ; Wei ZHU ; Guangyin PENG ; Hua HUANG
Chinese Journal of Postgraduates of Medicine 2012;35(15):35-37
ObjectiveTo study the expression and clinical significance of osteopontin in nasopharyngeal carcinoma tissues.MethodsOsteopontin expression was determined in nasopharyngeal carcinoma tissues in 44 cases and normal mucosa tissues in 25 cases by immunohistochemical staining.The relationship between osteopontin expression and pathological features of nasopharyngeal car(e)inoma was analyzed.ResultsThe positive expression rate of osteopontin in nasopharyngeal carcinoma tissues was significantly higher than that in normal tissues and the positive staining area was larger [77.27%(34/44) vs.8.00%(2/25),(60.24 ± 17.51 )% vs.(1.32 ± 0.48)% ](P<0.05).The positive expression of osteopontin was correlated with clinical staging and lymph node metastasis(P < 0.05),while had no relationship with pathological staging(P> 0.05).ConclusionsThere is a close correlation between expression of osteopontin and tumor cell invasion and metastasis.Over-expression of osteopontin may be one of the important factors contributing to the invasion and migration of nasopharyngeal carcinoma.
8.Two cases with crush syndrome complicated with pulmonary edema.
Sheng HAO ; Guang-hua ZHU ; Wei-xun HE
Chinese Journal of Pediatrics 2009;47(5):391-392
Child
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Crush Syndrome
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complications
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Female
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Humans
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Male
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Pulmonary Edema
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complications
9.Neoplasty of multiple cerebrospinal fluid rhinorrhea on combined frontal-nose approach through endoscope.
Wei-Yuan SUN ; Fu-Ming ZHU ; Xin-Hua XU
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2008;43(6):460-462
Cerebrospinal Fluid Rhinorrhea
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surgery
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Craniotomy
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methods
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Endoscopy
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Frontal Sinus
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surgery
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Humans
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Male
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Nose
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surgery
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Reconstructive Surgical Procedures
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methods
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Young Adult
10.Relationship of BRCA1 andβ-tubulinⅢmRNA expression with efficacy of taxanes for advanced esophageal carcinoma
Weimei JIANG ; Wei YE ; Hua JIANG ; Zhixia ZHU
Chinese Journal of Clinical Oncology 2013;(21):1288-1291
Objective:To investigate the relationship of the mRNA expression of BRCA1 andβ-tubulinⅢwith docetaxel-resistance in esophageal cancer. Methods:The genes BRCA1 andβ-tubulinⅢwere determined at the mRNA level using RT-qPCR in 36 esophageal carcinoma specimens. Results:The mRNA expression of BRCA1 andβ-tubulinⅢwas determined in the 36 tumor samples using RT-qPCR. The median BRCA1 mRNA expression level in relation to that ofβ-actin was 6.27. The medianβ-tubulinⅢmRNA expression level in relation to that ofβ-actin was 4.44. The patients were divided into two groups using these cutoff values. The BRCA1 mRNA expression level was not correlated with the sensitivity of esophageal cancer patients to docetaxel (P=0.733). The response rate of the tumors with highβ-tubulinⅢexpression was (38.9%), which is significantly lower than in patients with lowβ-tubulinⅢexpression (83.3%) (P=0.015). Conclusion:Theβ-tubulinⅢexpression levels in the tumor tissues were probably an important biomarker for the efficacy of docetaxel chemotherapy in esophageal cancer patients. Our study may provide new insights into taxane chemotherapy for advanced esophageal cancer patients.