OBJECTIVETo investigate whether bortezomib can enhance the sensitivity of human prostate cancer (PCa) cells to natural killer (NK) cell-mediated cytotoxicity, and whether it produces the same effect on different PCa cell lines.

METHODSWe treated androgen-dependent PCa LNCaP cells and androgen-independent PCa DU145 cells with bortezomib at the concentrations of 0, 5, 10, 15, 20 and 25 nmol/L for 24, 48 and 72 hours, and then detected the proliferation and apoptosis of the tumor cells by CCK-8 and Annexin V/PI, respectively.

RESULTSThe proliferation rates of the DU145 cells treated with 15, 20 and 25 nmol/L bortezomib were (82.79 +/-2.04)%, (73.59+/- 2.95)% and (74.16+/- 6. 16)% at 48 hours and (71.24+/- 5.30)%, (51.20+/- 2.91)% and (38.02+/- 2.67)% at 72 hours, and those of the LNCaP cells were (77.04+/- 7.74)% , (42.61 +/- 6.62)% and (23.85 +/-6.04)% at 48 hours and (36.45 +/-7.02)%, (14.94 +/-5.76)% and (11.65 +/-5. 87)% at 72 hours, both significantly inhibited as compared with the control group (P <0.05). At 24 hours, the apoptosis rates of the DU145 cells treated with 15, 20 and 25 nmol/L bortezomib were (14.41 +/- 1.32)% , (16.13 +/- 1.55)% and (14.48 +/- 1.42)% , and those of the LNCaP cells treated with 20 and 25 nmol/L bortezomib were (12.77 +/- 1.28)% and (14. 84 +/- 1.65)% , significantly higher than those of the control group (P <0.05) , and the DU145 cells showed an even higher sensitivity to bortezomib than the LNCaP cells. Bortezomib failed to sensitize these two cell lines to NK cell-mediated cytotoxicity in short-term assay, while long-term assay manifested that the apoptosis rates of DU145 and LNCaP cells after treated with 20 nmol/L bortezomib + NK cells were (41.83 +/- 5.06)% and (30.31 +/- 3.62)% , respectively, significantly higher

CONCLUSIONBortezomib enhances the sensitivity of than those after treated with either bortezomib or NK cells alone (P <0.05). PCa cells to NK cell-mediated cytotoxicity and adds to the effect of current cancer therapies, and it is more efficacious for androgen-independent prostate cancer.

Apoptosis ; drug effects ; Boronic Acids ; pharmacology ; Bortezomib ; Cell Line, Tumor ; Cytotoxicity, Immunologic ; drug effects ; Humans ; Killer Cells, Natural ; drug effects ; Male ; Prostatic Neoplasms ; pathology ; Pyrazines ; pharmacology

Angiotensin Ⅱ and type 1 receptor ( AT1R) are ab-solutely therapeutic targets in the hypertension and cardiovascular disease, and angiotensin Ⅱ gets involved in monocytes chemo-taxis and migration, T lymphocytes activation and proliferation, Th1 and Th17 immune function, and synoviocytes apoptosis inhi-bition in patients with rheumatoid arthritis ( RA) through binding to AT1R in the autocrine and/or paracarine manner. Drugs that limit angiotensin Ⅱ production by angiotensin converting enzyme inhibitors ( ACEIs) or blockade of angiotensin Ⅱ-AT1R action by AT1R blocker ameliorate immune and inflammation responses in RA patients. Additionally, angiotensin Ⅱ type 2 receptor (AT2R) expression increases in RA and experimental model, and activation of AT2R exerts a therapeutic effect on adjuvant-in-duced arthritis. AngiotensinⅡand its receptors in RA and auto-immune diseases are retrospectively reviewed in this paper.

Objective To investigate the effects of bortezomib on cellular migration and invasion ability in cuhured human prostatic cancer cells DU145 and its mechanism.Methods Transwell method was used to detect cell migration.Invasion was assayed by Matrigel-coated invasion chambers.Expressions of FAK and Tyr397 proteins were analyzed by Western blot.Results After treated by bortezomib at the concentration of 10,20 nmol/L for 24 h,the invasion index of DU145 cells were (69.05±10.56) and (52.55±6.98),they were gradually reduced compared with untreated group (81.55±10.56) (P ＜ 0.05).The migration index were (39.35±6.45),(32.05±4.22),they were also reduced compared with untreated group (58.75±5.41) (P ＜ 0.05).The group of treated by bortezomib showed Tyr397 protein expression had been suppressed.However FAK protein had not marked change.Conclusions FAK is involved in the regulation of cellular migration and invasion function.Bortezomib might inhibit cells migration and invasion function by down regulation of Tyr397 expression.

Adrenergic beta-Agonists ; therapeutic use ; Aged ; Albuterol ; analogs & derivatives ; therapeutic use ; Bronchi ; drug effects ; physiopathology ; Bronchodilator Agents ; therapeutic use ; Coal Mining ; Humans ; Male ; Middle Aged ; Pneumoconiosis ; drug therapy ; physiopathology ; Respiratory Function Tests ; Salmeterol Xinafoate ; Treatment Outcome

Objective:To observe the application effect of continuous blood purification(CBP) machine in treatment of multiple organs dysfunction syndromes(MODS) in children.Methods: 26 cases of children with MODS implemented CBP therapy were choosed as the research objects. All the patient’s treatment time, replacement liquid dosage, the quantity of liquid ultrafiltration were recorded. All the patient’s blood pressure and heart rate were monitored before and after CBP therapy. The changes in the renal function indices such as BUN and SCr, the blood gas indexes such as pH, PaO2, PaCO2, HCO3-and BE, the electrolyte indicators such as Na+, K+, Cl- and osmotic pressure were observed and compared before and after CBP therapy.Results: (1)The average period of treatment of 26 cases in children with MODS was (77±53)h. The average amount of fluid replacement was (18.4±15.8)L/d, and the average quantity of liquid of ultrafiltration was (2630±1760)ml/d. The vital signs of all the children were stable in the process of CBP therapy, and the blood pressure and heart rate had no obvious changes before and after treatment. (2)The renal function indices such as BUN and SCr decreased obviously after treatment. The differences were statistically significant (t=50.392,t=96.115;P<0.05). The blood gas indexes such as PH, PaO2/FiO2, HCO3- and BE were improved significantly after treatment (t=4.450,t=20.105,t=11.209,t=54.840;P<0.05). The electrolyte indicators such as Na+, K+, Cl- and osmotic pressure had no obvious change after treatment (P>0.05).Conclusion: CBP therapy can effectively reduce various inflammatory mediators and toxin, improve renal function, help to stabilize the environment in the body and it is an effective, safe and reliable therapeutic method in the treatment of children with MODS.

To evaluate the safety and efficacy of transrectal high-intensity focused ultrasound (HIFU) in the treatment of benign prostatic hyperplasia (BPH), serial studies were conducted in 150 BPH patients before and 30 min, 1, 2, 6 and 12 month(s) after Sonablate-500 HIFU treatment. A silicon-coated indwelling 16F latex catheter was placed during the determination of the therapy zone. Preoperative and postoperative evaluations were made by using the international prostate symptom score (IPSS), quality of life (QOL), uroflowmetric findings and transrectal ultrasound, and incidence of complications. The cystourethrography was done in 23 patients within 1 year postoperatively. The results showed that after HIFU treatment, IPSS and QOL scores were significantly decreased at 1, 2, 6 and 12 month(s) (P<0.01). Maximum urine flow rate (6.0 to 17.2 mL/s, P<0.01), PVR (75.0 to 30.3, P<0.01) and prostatic volume (65.0 to 38.1 mL, P<0.05) were significantly improved 12 months after the operation. Recurrent urinary retention (n=2) and urethrorectal fistula (n=1) occurred at the 15(th) postoperative day. The duration of the HIFU prostate ablation was 25-90 min. The mean time for an indwelling catheter was 3-19 days. These data demonstrate that treatment of BPH with Sonablate-500 HIFU is safe and effective.
Prostatic Hyperplasia/diagnosis ; Prostatic Hyperplasia/*therapy ; Ultrasound, High-Intensity Focused, Transrectal/adverse effects ; Ultrasound, High-Intensity Focused, Transrectal/instrumentation ; Ultrasound, High-Intensity Focused, Transrectal/*methods

Objective]Analysis about how Dai Sigong expounded and treated diarrhea. [Methods]Look up relevant literature,list Dai Sigong ’s expositions about diarrhea’ pathogenesis and treatments, and summarize how Dai Sigong treated diarrhea. [Results] Dai Sigong generalized some methods about diarrhea treatment, such“diaphoresis”“purgative method”“emesis method”“tonifying method”“harmonyzing method”“invigorating spleen for diuresis”“invigorating qi for ascending”“eliminating”dampness”“clearing interior heat”and so on.[Conclusion]Depanding on predecessors’ discourse analysis, Dai Sigong inherited Zhu Danxi’s academic thought, summariied diarrhea’ pathogenesis and treatments, made a breakthrough to a thought that“It’s noneffective not using diuresis to eliminate dampness”and put forword that“there are many ways to cure diarrhea by eliminating dampness”. It provided more means to therapy diarrhea for descendants and had great meanings to consult.

Objective To observe the effect and mechanism of quercetin on the proliferation of rat hepatic stellate cell(HSC)induced by TGF-β1.Methods HSC was isolated by orthotopic hepatic perfusion with collagenase Ⅳ.MTT assay was used to detect the inhibitory effect of quercetin on the proliferation of HSC.The expression of typeⅠcollagen in HSC was detected by ELISA.The effect of quercetin on the expression of typeⅠcollagen,Smad2 and Smad7 mRNA in HSC was detected by real-time quantitative PCR.The expression of type Ⅰ collagen,Smad2 and Smad7 protein in HSC was detected by Western blot.Results Quercetin could inhibit the proliferation of HSC,down-regulated the expression of type Ⅰ collagen,Smad2 mRNA and protein,and Smad7 mRNA and protein expression were significantly up-regulated(all P<0.05).Conclusion The anti-hepatic fibrosis effect of quercetin may be related to its blocking TGF-β1/Smad pathway of HSC and blocking the activation and proliferation of HSC.

Objective To explore the treatment and clinical characteristic of posterior mini-incision surgery for the single segment of the lumbar disc herniation.Methods Clinical data of 260 cases with single segment lumbar disc herniation were analyzed retrospectively.Results Follow-up for 6 months to 5 years,with an average of 24 months.According to Nakai effect criteria,109 cases of excellent,good in 132 cases,17 cases of fair,two cases of poor,12 cases of cerebrospinal fluid leakage,2 cases of wound hematoma,one case of vertebral space infection,resulting in 92.7%.Conclusion Advantages of posterior mini-incision surgery for the single segment of the lumbar disc herniation are small incision,less trauma,shorter operative time,less bleeding,quick recovery and low costs.Stressed the need to accuratel position preoperation.

Nowadays partial splenic embolization (PSE) is an important therapeutic means to treat secondary hypersplenism caused by portal hypertension. By reducing the splenic blood flow and increasing hepatic blood supply, the peripheral blood picture and the liver function indexes as well as the portal hypertension can be effectively improved. This article aims mainly to make a comprehensive review on the therapeutic effect and mechanism of PSE, its clinical efficacy, as well as the factors affecting the clinical results, and to discuss the postoperative complications, the advantages and disadvantages of different embolic materials.