OBJECTIVE: To establish a method for the content determination of calycosin-7-O-?-D-glucoside,prim-o-glucosyl cimifugin and 4′ -O-?-D-glucosyl-5-O-methylvisammin in Yupingfeng decoction and to study the influence of different combination on the content of three compositions.METHODS:HPLC was applied and the determination was performed on Hypersil ODS(250 mm?4.0 mm,5 ?m)column.The mobile phase consisted of acetonitrile-water(gradient elution)with detection wavelength of 254 nm.RESULTS: The content of the three compositions represented little change or different deceasing tendency in the decoctions of various compatibilities.The biggest deceasing tendency of content was found in the decoctions containing Astragalus membranaceus,Saposhnikovia divaricata,and Atractylodes macrocephala.CONCLUSIONS: The content of the three compositions of different combination shows a dynamic course,which provides a reference for the rules of the compatibility and material basis of Yupingfeng decoction.

OBJECTIVE:To study the chief chemical components of the ethyl acetate part of Angelicae Sinensis Decoction for Supplementing Blood Apozem.METHODS:The HPLC was adopted in which Hypersil ODS was taken as the chromato?graphic column,the Methanol-0.5%glacial acetic acid was taken as the mobile phase(gradient elution),the detective wave?length was280nm,the flow rate was1ml/min and with the column temperature set at room temperature.RESULTS:There were7characteristic peaks in the ethyl acetate part of Angelicae Sinensis Decoction for Supplementing Blood Decoction,6of which come from isoflavone of Radix Astragali and1come from ferulic acid of Angelicae Sinensis.CONCLUSION:The chief characteristic peak of the ethyl acetate part of Angelicae Sinensis Decoction for Supplementing Blood Apozem was the building up of Radix Astragali peak and angelicae sinensis peak,no other significant composition peaks were emerged.

OBJECTIVE: To establish the GC fingerprints for Grassleaf sweetflag rhizome aetherolea and to control its quality .METHODS: The temperature at the mouth of the sample injector was 250℃ and that of the detector was 280℃, the carrier gas was nitrogen gas and the flow rate was 1.3ml/min, the GC of aetherolea in 10 batches of Grassleaf sweetflag rhizome was analyzed by adopting temperature programming.RESULTS:Altogether 6 peaks were marked out,the sum total of the average peak area of which made up(75.4?13.7)% of the total.CONCLUSION: The method is of high degree of precision, reproducibility,stability,the resolving of each component in the aetherolea is good,the established fingerprints can be used as one of the quality control index for Grassleaf sweetflag rhizome.

AIM: To establish the method of fingerprint analysis in Herba pogostemonis by HPLC. METHODS: HPLC with Hypersil ODS column was used,taking Methanol-0.05% phosphoric acid(gradient elution)as a mobile phase and detection wavelength was set at 270nm. RESULTS: 26 peaks were separated on HPLC fingerprint in Herba pogostemonis. CONCLUSION: The method is reliable,accurate and can be used as a quality control method for Herba pogostemonis.

Objective To establish the method of fingerprint analysis on the fruits of Amomum villosum by HPLC and work out the characteristic fingerprint of methanol extracts in the fruits of A.villosum from Yangchun,Guangdong Province as index component which could be used for the accumulation of data to evaluate the inner quality of the fruits of A.villosum.Methods HPLC with Nucleodur C18 Gravity column was used,the methanol-2% acetic acid(gradient elution)as a mobile phase,detection wavelength at 260 nm,column temperature was 30 ℃,and flow rate was 1.0 mL/min.ResultsCommon peaks(20)were separated on HPLC fingerprint in A.villosum.The similarities of 18 batches of samples were higher.Conclusion The method is reliable and accurate,has a better reproducibility,and provides a reference for the quality control to the fruits of A.villosum.

OBJECTIVE:To establish the method for identification of Changyanling tablet and determination the content of berberine in the tablet METHODS:Rhizoma coptidis,Radix paeoniae alba and Rhizoma corydalis in Changyanling tablet were qualitatively identified by thin layer chromatography method The content of berberine was determined by the TLC-scanning RESULTS:The average recovery of berberine was 98 19% with a RSD of 0 93% CONCLUSION:This method is simple,accurate and reliable,and can be used for the quality control of this preparation

ObjectiveTo assess activated carbon in the standard lymphadenectomy of gastric cancer.Methods183 cases undergoing radical gastrectomy before 1997 served as control group,87 consecutive cases after 1997 received intumour injection of activated carbon(AC) before undergoing gastrectomy.The areas and number of dissected lymph nodes,operative complication and hospital stay in subtotal gastrectomy and total gastrectomy were analyzed respectively.ResultsIn subtotal gastrectomy,the ratio of D 1 ,D 2 ,D 3 and D 3 +PAL over the total cases was 4 7%,10 3%,14 9% and 16 1% respectively in AC group and 25 7%,21 3%,9 9% and 2 7% respectively in control group.In total gastrectomy,the ratio of D 1 ,D 2 ,D 3 and D 3 +PAL was 3 4%,5 7%,21 9% and 23% respectively in AC group and 15 8%,14 8%,8 7% and 1 1% respectively in control group.The differences of the four procedures were all statistically significant between the two groups.The number of dissected lymph nodes in each case in AC group was more than that of control group.ConclusionsLocal injection of activated carbon is a simple,easy and safe method to increase the dissection of lymph nodes in radical gastrectomy.

AIM:To establish the method of fingerprint analysis on Yupingfeng Decoction(Radix Astragali,Radix Saposhnikoviae,Rhizoma Atractylodis macrocephalae),work out the characteristic fingerprint,and study the influence of various compatibilities on fingerprint peaks.METHODS:HPLC with Hypersil ODS was used,acetonitrile-water(gradient elution)as a mobile phase and detection wavelength was at 220 nm,flow rate was 1 mL/min,and column temperature was at 30 ℃.RESULTS:11 common peaks were separated in 10 batches of Yupingfeng Decoction.A little influence on characteristic peaks was found in various compatibilities,but there was no new characteristic peak.The characteristic peaks were the summabilty,peak 2,5,6,8,9,10 were from Radix Astragali,peak 1,3,4,7 were from Radix Saposhnikoviae and peak 11 was from Rhizoma Atractylodis macrocephalae.CONCLUSION:The method is reliable,accurate and provides for further reference compatibility and material base of Yupingfeng Decoction.

Objective To establish the method of fingerprint analysis on Yupingf eng Decoction,and to study the correlation of HPLC fingerprint in Radix Astraga li,Radix Saposhnikovlae,Rhizoma Atractylodis Macrocephalae and Yupingfeng Deco ction. Methods HPLC with Hypersil ODS was used,acetonitrile -water(gradient el ution) as a mobile phase and detection wavelength at 220 nm,flow rate was 1 mL ?min-1,and column temperature was 30 ℃. Results There were 9,8 and 7 common peaks separated from 10 batches of medicinal material of Radix Astragali,Radix Saposhnikovlae,and Rhizoma Atractylodis Macrocephalae,respectively;11 common peaks were separated from 10 batches of Yupingfeng Decoction,of which 6 peaks were shared by Radix Astragali,4 peaks by Radix Saposhnikoviae and 1 peak by Rh izoma Atractylodis Macrocephalae. Conclusion There exists a correlation of Yupin gfeng decoction with the medicinal pieces of Radix Astragali and Radix Saposhnik oviae. The major characteristic fingerprint peaks of Yupingfeng decoction belong to those of the isoflavones from Radix Astragali and chromones from Radix Sapos hnikoviae. This will provide a reference for the rules of the compatibility and component research of Yupingfeng Decoction.

AIM:To establish the method of fingerprint-analyzing Herba pogostemonis by HPLC,and compare the variability of the different parts,including stems and leaves,which could be used for quality evaluation of Herba pogostemonis. METHODS: HPLC with Hypersil ODS column was used,the acetonitrile-0.05% phosphoric acid(gradient elution)as a mobile phase and detection wavelength was at 320 nm,column temperature was at 30 ℃,and flow rate was 1.0 mL/min. RESULTS: 16 common peaks were separated on HPLC fingerprint in the stems and leaves of Herba pogostemonis,there was significant variability between the stems and leaves,the content in the leaves was more than in the stems. CONCLUSION: The method is reliable,accurate and provides a reference for the quality control of Herba pogostemonis.