Objective To investigate the changes of psychological state and family environment of the patients with neurosis (including depression and anxiety) in the process of treatment. Methods The Hamilton depression scale (HAMD), Hamilton anxiety scale(HAMA), self-rating depression scale(SDS), self-rating anxiety scale(SAS), family environment scale(FES), and family adaptability and cohesion scale(FACESⅡ-CV) were used to evaluate the changes of psychological state and family relationship in the process of treatment among patients with depression and anxiety hospitalized in general hospitals (n=45) and their family members (n=45). Results Before intervention, values of HAMD, HAMA, SDS and SAS in patients were higher than normal ones, and were in line with the corresponding diagnostic criteria. After psychological intervention and medication for 2 to 3 months, symptoms such as depression and anxiety were improved significantly, and values of the above scales were within normal ranges. Besides, there was also significant improvement in FES and FACESⅡ-CV after intervention among the patients. All the values of the family members were in the normal ranges before intervention, however, significantly positive changes were observed after intervention. Conclusion With the improvement in depression and anxiety after intervention among patients, the psychological pressure of family members is released, and family relationship is partially restored. The rehabilitation of patients plays an important role in the restoration of family function.

Objective:To observe the curative effects of the combined application of domperidone, pantoprazole and hydrotalcite tablets in the treatment of bile reflux gastritis ( BRG) and explore the underlying mechanism. Methods:Totally 80 patients with BRG were randomly divided into the treatment group and the control group with 40 cases in each. The treatment group was received panto-prazole, hydrotalcite tablets and domperidone, and the control group was treated by hydrotalcite tablets and domperidone. The efficacy was observed in both groups after 4 weeks. Results:The effective rate of symptom relief in the treatment group and the control group was respectively 92. 5% and 62. 5% (P<0. 05). After the 4 week treatment, the gastritis healing rate in the treatment group was higher than that in the control group (P<0. 05). The improvement of gastric mucosal hyperemia, edema and erosion in the treatment group was better than that in the control group with significant difference (P<0. 05). Cholic acid in gastric juice and gastric acid se-cretion were significantly reduced in both groups after the treatment, while the reduction of gastric acid secretion in the treatment group was more notable than that in the control (P<0. 05). Conclusion:Pantoprazole can be used to control BRG, and the combination of the three drugs shows better efficacy in the treatment of BRG.

To investigate the different expression of Fas, Fas L and Caspase 3 in hypertrophic scars and normal skins in order to explore their influences on scar formation, the expression intensity and distribution of Fas, Fas L and Caspase 3 were detected by immunohistochemistry and pathological methods in 8 cases of hypertrophic scars and 8 cases of normal skins. In normal skins, Fas and Fas L were mainly located in the cellular membranes and cytoplasms of epidermal cells and some fibroblasts. Caspase 3 was mostly distributed in epidermal basal cells and some fibroblasts. In hypertrophic scars, Fas and Fas L were principally existed in the membranes and cytoplasms of epidermal keratinocytes. The positive cellular ratio of two proteins was significantly reduced ( P

The purpose of this study was to investigate the localization and expression characteristics of phosphorylated form of extracellular-signal regulated-protein kinasel/2 (p-ERKl/2), Ras and C-fos in skin at different development stages and to explore their potential biological significance. Immunohistochemistry and pathological methods were used to detect the expression intensity and distribution of p-ERKl/2, Ras and C-fos in skin of 8 fetuses with different gestational ages (13 to 31 weeks) and 8 adults. Positive immunohistochemical signals of p-ERK1/2, Ras and C-fos. could be found in fetal and postnatal skins. Along with the increment in gestational age, the positive cell rates of p-ERK1/2, Ras and C-fos in the skin elevated progressively. In skins derived from the fetus of late-trimester pregnancy and adult, the positive rates of these three proteins were significantly increased in comparison with skin from the early-trimester fetus (P

Localization and expression of two subunits (IGF ⅠR? and IGF ⅠR?) of insulin like growth factor Ⅰ receptor and phosphorylated tyrosine proteins (P Tyr) in skins at different developmental stages were studied in order to explore their potential biological significance. Immunohistochemistry and pathological methods were used to detect the expression intensity and distribution of IGF ⅠR?, IGF ⅠR? and P Tyr in skins of 12 fetuses with different gestational ages and 8 adults. The results showed that positive immunohistochemical signals of IGF ⅠR?, IGF ⅠR? and P Tyr could be found in fetal and adult skins. Along with growth and development of the fetus, the positive cell rates of IGF ⅠR?, IGF ⅠR? and P Tyr in skins elevated progressively. In adult skins, IGF ⅠR was mainly located in the cell membrane of epidermal cells, while P Tyr was chiefly distributed in epidermal cells and some fibroblasts. These results suggested that IGF ⅠR and its mediating signaling pathway might be involved in cutaneous development at embryonic stage, in cutaneous structure and function maintenance, and in wound healing at postnatal stage.

To explore the change in gene expressions of p38 mitogen-activated protein kinase (p38MAPK) and its upstream signal-regulated molecule (mkk3 and mkk6) in normal skin versus hypertrophic scars underlying its potentially biological significance. Total RNAs were isolated from 8 specimens of hypertrophic scars and 8 specimens of normal skin, then they were purified to mRNAs, and the gene expression of mkk3, mkk6 and p38MAPK was examined with reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the intensities of gene expression of mkk6 and p38MAPK were weak in normal skins, while the expression of these 2 genes was significantly elevated in hypertrophic scars compared with normal skins (P0.05). In hypertrophic scar, the elevation of mkk6 and p38MAPK gene expression, which plays pilot roles in cell proliferation, may be one of the mechanisms controlling the formation of hypertrophic scars.

To investigate the effect of FGF-10 on the secretion of GM-CSF by adult keratinocytes in vitro and to understand the mechanisms involved in the stimulation of granulation tissue formation by FGF-10 during wound healing. Concentrations of FGF-10 used were 4, 16, 125 and 500 ng/ml. Cells were seeded in the amount of 2 500 cells/cm 2 or 5 000 cells/cm 2 in dishes in serum-free medium, and supernatants were collected at 24, 48 and 72 hours after culture. The amounts of GM-CSF in cell culture supernatants were determined using GM-CSF ELISA kits, and cell numbers were counted by haemocytometer. For cells seeded in low density (2 500 cells/cm 2), GM-CSF was not detected at 24 hours. At 48 hours, both in absolute concentrations and on a per-cell basis, the amounts of GM-CSF secreted in cultures with 125 and 500ng/ml FGF-10 were significantly higher than that in negative control (P0.05). At 48 hours, the keratinocytes in the middle area were confluent, and a number of cornified cells were observed, while the productions of GM-CSF in FGF-10 cultures were not higher than that in negative control. There was a clear negative correlation between the secretion production of the growth factor and the total cell number in each dish with a correlated coeffecient of 0.881 (P

Objective To study expression intensity and distribution of phosphorylated forms of extracellular signal-regulated protein kinase1/2 (ERK1/2), stress-activated protein kinase (SAPK) and P38MAPK in normal skin and hypertrophic scars and their potential biological significance. Methods The morphological characteristics of hypertrophic scars in different periods after wound healing and normal skin were examined histopathologically. Protein expression of p-ERK1/2, p-SAPK and p-P38MAPK was also assessed with immunohistochemical technique. Results In normal skin, phosphorylated forms of ERK1/2, SAPK and P38MAPK were mainly located in epidermal basal cells, in which the positive cellular rates of all the three proteins were low. Along with the maturation of hypertrophic scars, protein contents of p-ERK1/2 and p-SAPK were progressively increased. In mature hypertrophic scars, positive signals of these two proteins were mostly distributed in keratinocytes and some fibroblasts. The positive cellular rate of p-P38MAPK ascended in active hypertrophic scar, then decreased to a level which was still higher than that of normal skin. Conclusion The formation of hypertrophic scars may be associated with the alteration in signaling pathways which results in the increment of protein contents of p-ERK1/2, p-SAPK and p-P38MAPK.

Objective To investigate gene expression of epidermal growth factor (EGF), its receptor (EGFR) and two protooncogenes (c-fos and c-myc), in fetal skin at different development stages and children skin and to delineate their potential biological significance. Methods Fetal skin biopsies of human embryo were obtained from spontaneous abortions at different gestational ages from 13 to 32 weeks and children skin specimens were collected from patients undergoing plastic surgery. After morphological characteristics of skin at different development stages were detected with histological methods, gene expressions of EGF, EGFR, c-fos and c-myc in skin at different development stages were examined with reverse transcription-polymerase chain reaction analysis (RT-PCR). Results Gene expression of EGF, EGFR, c-fos or c-myc could all be detected in fetal and childern skins. In fetal skin, the gene expression of these 4 genes was weak. Gene expression of these genes in skin was progressively enhanced with increasing gestational age. In children skin, the mRNA contents of these 4 genes were significantly increased in comparison with those in fetal skin (P

Objective To comprehensively analyse the transcriptional changes of genes and their biologic significance that occurred in the process of development of human fetal skin by using high-density oligonucleotide DNA array. Methods Samples of human fetal skin were obtained from aborted fetuese of 10W, 15W, 24W, 32W EGA (estimated gestational age) respectively. Total RNAs were isolated from of skin specimens of fetuses of different EGA, and mRNAs were purified and labeled with incorporation of fluorescent dUTP to prepare the hybridization probes by using reverse transcription polymerase chain reaction (RT-PCR). Approximately 21 329 human genes were spotted on a chemical-material-coated-glass plate in array. Results According to the hybridization results from oligonucleotide DNA microarray, gene expresion patterns and functions were analysed. Gene-chip disclosed a large scale of information in developmental human fetal skin, rendering a convenient way to investigate the temporal and spatial expressions of gene profile among skin cells. Many specific genes transcription expressed differently at different stages of development of fetal skin, suggesting their key roles in development, differentiation and regulation. Conclusions Microarray or DNA chip technology has revolutioned biological research by empowering to broaden the scope of collecting genomic information. Therefore, microarray-based study is able to reveal a substantial number of genes which might participate in embryogenesis and development of human skin. The present study demonstrated a previously unrecongnized role of gene expression in the control of human fetal skin growth and structure during its developmental stage. A complicated network of skin development process was fairly well characterized.