OBJECTIVE: To evaluate the features and application of tissue engineered biomaterials in repairing femur defects, and to search an optimal femur substitution.METHODS: Papers published between January 1993 and October 2009 were searched using computer with key words of "tissue engineering, femur defect, mesenchymal stem cells, cytokines and materials" both in English and Chinese. Documents addressing biomaterials and tissue engineered bone in repairing femur defects were included. Repetitive research or Meta analysis was excluded. After that, 21 literatures were selected to discuss the features and application of tissue engineered biomaterials in repairing femur defects.RESULTS: Stem cells technology is obtaining seed cells from myeloid tissues, amplifying in vitro, combining with scaffold material, and in vitro constructing tissue engineered bones. The cultured cells could maintain osteoblast biological characteristics and fully fused with bone tissues at recipient sites, simultaneously, avoid immunological rejection. The combination of materials or modify the material surface could promote cells adhere to materials and increase the cellular biological activity. When transfecting seed cells to bone defect areas using certain carriers, the transplanted calls could form new bones and secret bone growth factor, induce determined osteogenic precursor cells differentiated into inducible ostegenic precursor cells, thereby, fasten the bone healing. The composite materials prepared by three-dimensional virtual surgical simulation and computer numerical control exhibited advantages of composite materials and histological anatomy conjunction, which had perfect appearance.CONCLUSION: Currently, there is no material can fully meet the requirement of bone tissue engineering. Thus, promoting adhesion between cells and materials, increasing cellular biological activity, and maintaining biological functions by combing materials or modifying material surface are the research focuses.

Humans ; Laryngeal Diseases ; prevention & control ; Larynx ; Pharyngeal Diseases ; prevention & control ; Pharynx ; Pulmonary Disease, Chronic Obstructive ; prevention & control

Decision Making, Organizational ; Disasters ; Earthquakes ; Humans ; Public Health Practice ; Stress Disorders, Post-Traumatic

BACKGROUND: Reports of culture condition of olfactory ensheathing cells (OECs) vary. And some methods have bad reproducibility, not appropriate for actual application.OBJECTIVE: To observe the effect of differential attachment, chemicals in combination with trypsin digestion to purify rat OECs in vitro and to compare the effect with differential attachment or chemicals alone.DESIGN, TIME AND SETTING: In vitro controlled observation of cytology was performed at the Laboratory of Department of Human Anatomy & Histology and Embryology, Fujian Medical University from June 2007 to June 2008.MATERIALS: A total of 8 SD rats, 2 days old, were provided by the Laboratory Animal Center of Fujian Medical University.METHODS: The OECs were dissociated from the postnatal rat olfactory bulbs under sterile condition, and seeded in poly-L-lysine-coated culture flask at a density of 4.0×10~8 /L for primary culture. The cells were divided into 4 groups: control group (not purified); chemicals group (5 μmol/L arabinose); differential attachement group (Nash differential attachement); combination group (Nash differential attachement to eliminate most of the fibroblasts, followed by arabinose; when the cells were confluent at 6 days, the cells were digested with 1.25 g/L trypsin for 1 minute until the processes were shrank, cells became round, with some cells floating).MAIN OUTCOME MEASURES: Morphological changes of the cultured OECs and NGFRp75 immunocytochemistry were observed.RESULTS: The OECs displayed a very characteristic morphological appearance. Most of OECs were bipolar or tripolar with long and slim processes. In the unpurified control group the rapidly proliferating fibroblasts were in the majority (60%) after 7 days in culture, and confluent at day 14. The OECs occupied the most in the other groups, and their appearance remained unchanged.The surviving bipolar or tripolar OECs were positive for NGFRp75. The purifity by chemicals and differential attacehment methods was low (75%), while the combination group was high (85%).CONCLUSION: The method of purifing OECs through a combination of differential attacehment, chemicals and trypsinization is effective.

Objective To observe the preemptive analgesia effect of parecoxib sodium in patients undergoing laparoscopic cervical carcinoma radical resection.Methods Seventy patients undergoing laparoscopic cervical carcinoma radical resection were randomly divided into 2 groups with 35 cases each. Parecoxib sodium 40 mg was injected intravenously 10 min before operation and repeatedly given every 12 h. Equal volume physiological saline was given at same time in the control group. Two groups received postoperative PCIA with morphine. The numerical rating scale （NRS） was used to rate pain intensity at following time points：immediately after extubation,2,6,12,18,and 24 h after operation. Twenty-four hour morphine consumption and side effects were recorded.Results The NRS rating of pain at each time point in the parecoxib group was significantly lower than that of the control group （P0.05）,and the total morphine consumption （10.4±7.6）mg was less than the control （17.7±8.9）mg （P0.05）; correspondingly,the incidences of nausea,vomiting and drowsiness were less,and the number of patients left bed for activity was increased in the parecoxib group than those in the control one （P0.05）. Conclusion Preoperative parecoxib sodium 40 mg can improve the analgesic effect of PCIA with morphine,and reduce morphine consumption and the incidences of side effects.

Objective To investigate the significance of pulse indicating the continuous cardiac output (PICCO) technique in patients with post-trauma capillary leak syndrome (PTCLS). Methods Twenty traumatic patients with PTCLS received PICCO monitor from 2011 to 2014 were enrolled in this study. analyzed, The patients, with twelve males and eight females, aged from twenty three to fifty. And twenty patients in the control group, with ten males and ten females, aged from twenty one to fifty two. Twenty six patients were injuried by traffic, ten patients were injuried by crash and four patients were injuried by sword. No significant differences were found in age、gender、weight、scores of ISS、Murray and APACHEⅡof all patients. All patients were survived. The balance of fluids, Lactate, Central venous oxygen saturation (ScvO2) and the mean artery pressure (MAP) were compared at 0, 24, 48 and 72 h after entering into the ICU. The duration of ICU stay and ventilation were also compared. Results (1)No significant differences in MAP、Lac and ScvO2 were observed in two groups. (2)No significant differences in the balance of fluids when patients entered into ICU, but significant differences were found at 24, 48 and 72 h post-entering into ICU (P < 0.05). (3)Significant differences in the duration of ICU stay and ventilation between the two groups were observed (P < 0.05). Conclusion PICCO technique can provid a quantitative target for PTCLS patients, with decreasing fluid infusion, and reducing the duration of ICU stay and ventilation.

Objective To study the relationship between balance function and attention in children with attention deficit hyperactivity disorder(ADHD),and guide the clinical intervention and training of ADHD.Methods The Tetrax balance system and the CPT were conducted among 124 ADHD children (age 6.0-14 years) and the normal controls.Results (1) The balance function was different between ADHD and normal controls.In junior grade of elementary school,the Fourier score of ADHD children was significantly higher than that of the normal control group in F1,F2-F4 and F5-F6 (P＜0.05).In senior grade of elementary school,the Fourier score of ADHD children was significantly higher than normal controls in F2-F4 and F5-F6 (P＜ 0.05).There was no significant difference between ADHD and normal controls in junior high school.(2) There was correlation between balance function and attention in children with ADHD.In junior grade of elementary school,the full scale attention quotient was negatively correlated with F2-4,F5-6,F7-8 (P=0.018,0.009,0.010; rs =-0.146,-0.203,-0.189),and the visual attention quotient were negatively correlated with F1,F2-4,F7-8 (P =0.043,0.039,0.046 ; r s =-0.062,-0.071,-0.112).In senior grade of elementary school,the full scale attention quotient was negatively correlated with F5-6,F7-8 (P=0.015,0.033; rs =-0.217,-0.172),and the visual attention quotient were negatively correlated with F1,F7-8(P=0.037,0.045; rs =-0.051,-0.101).In junior high school,the negative correlation was existed between the visual attention quotient with F7-8 (P=0.038,r s =-0.114).Conclusion ADHD children have deficit balance.The balance function of ADHD children matures gradually.There is internal connection between balance and attention,especially for visual attention.

AIM: By comparing the aspheric and multifocal Toric intraocular lens and the aspheric intraocular lens in cataract patients,to observe the patients` visual quality,to evaluate the patients` distance visual acuity,near visual acuity,Pseudo-accommodative power,residual astigmatism,the rotational stability of AcrySof IQ Toric Restor intraocular lens (IOLs),contrast the rate of off-distance glass,the rate of off-near glass and patient satisfaction after the implantation of AcrySof IQ Toric Restor IOL in the cataract patients with preexisting corneal astigmatism.METHODS:Carried out in 46 patients (48 eyes) with age-rated cataract who receiving phacoemulsification and implantation of artificial lens in our department during February 2014 to June 2016.All the cases were randomly divided into 2 groups: experimental group: 23 cases (24 eyes),AcrySof IQ ReSTOR Toric IOL was implanted after phacoemulsification;control group: 23 cases (24 eyes),AcrySof IQ was implanted.Uncorrected distance visual acuity (UCDVA) and best corrected distance visual acuity (BCDVA),uncorrected near visual acuity (UCNVA) and best-corrected near visual acuity(BCNVA),Pseudo-accommodative power,residual astigmatism,preoperative and postoperative corneal astigmatism,the rate of off-distance glass,the rate of off-near glass and patient satisfaction were measured at 6mo postoperatively follow-up.RESULTS: BCDVA and BCNVA were not statistically different between the two groups(P<0.05).At 1wk,1,3 and 6mo postoperatively of UCDVA the experimental group were statistically better than the control group (P<0.05).UCNVA were also statistically better than the control group (P<0.05).The mean residual astigmatisms 1wk,1,3 and 6mo postoperatively were statistically differences between the control group and experimental group (P<0.05).At 6mo postoperatively,patients satisfaction of experimental group were significantly better than control group (P<0.05).Spectacles independence for near vision achieved 83% and 17% in experimental group and control group,experimental group were statistically better than control group(P<0.05).Spectacles independence for distance vision achieved 92% and 67% in experimental group and control group,experimental group were statistically better than control group(P<0.05).CONCLUSION:The results indicate that AcrySof IQ ReSTOR Toric IOLs may provide a better uncorrected distance visual acuity and uncorrected near visual acuity,which can significantly reduce preexisting corneal astigmatism in cataract surgery,improve spectacles independence for distance vision and spectacles independence for near vision,have a high patient satisfaction.

Objective To study the value of laparoscopic cholecystectomy (LC) for treating gallbladder benign diseases in aged patients. Methods Laparoscopy cholecystectomy (3-port or 4-port) was performed in 2335 senile patients with gallbladder benign diseases from December 1992 to May 2005. Results Five patients were found as having the Mirizzi syndrome and were given a subtotal cholecystectomy. In 18 patients with a frozen Callot’s triangle, the anterior wall of the gallbladder was resected and the mucous membrane on the posterior wall was electrocauterized. LC was completed smoothly in the remaining 2312 patients. No conversions to open surgery were needed. The operation time was 8~55 min (mean, 19 min), and the intraoperative blood loss was 1~ 50 ml (mean, 8 ml). An abdominal drainage tube was placed in 213 patients, including double-cannula lavage in 56 patients. The length of hospital stay was 4~16 d (mean, 6 d). Follow-up examinations for 10 days ~13 years in 1859 patients showed no biliary stenosis, gallbladder pouch, or residual stones. Conclusions Laparoscopic cholecystectomy is safe and feasible in aged patients, being the first choice of operative method. Proper perioperative management, careful prevention co-morbidities, and control of operation time as short as possible are important for senile patients.

Objective To investigate the effect of somatostatin on pancreatic blood flow and pancreatic function on the patients with acute pancreatitis.Methods 120 patients with acute pancreatitis from March 2015 to March 2017 were collected, and were randomly divided into the observation group and the control group.The control group were given routine treatment, at the basis of this, the observation group were given somatostatin.octreotide 0.6 mg was dripped into intravenous continuously, and was reducted to 0.3 mg, continuous treatment for 7 d.The changes of blood flow and the curative effect of pancreas were compared between the two groups before and after treatment.Results The total effective rate was 96.67%(58/60)in the treatment group and 80.00%(48/60)in the control group.The total effective rate in the observation group was significantly higher than that in the control group, the difference was statistically significant (P<0.05).There was no significant difference in blood flow(BF), blood volume(BV)and capillary surface permeability(PS)between the two groups before treatment.1 and 7 days after treatment, BF, BV in the observation group were higher than those in the control group, the levels of PS were significantly lower than that in the control group, the differences were statistically significant(P<0.05).Before treatment, the differences of(CP), insulin(INS), glucagon(GLU), serum lipase(LIP), amylase(AMY)and trypsinogen-2(TPS)were measured in the two groups were not statistically significant.1 and 7 days after treatment, CP and INS in the observation group were significantly higher than those in the control group, and GLU, LIP, AMY and TPS-2 were significantly lower than those in the control group, the differences between the two groups was statistically significant (P<0.05).Conclusion It has a protective effect on pancreatic blood flow and function which somatostatin was used in the treatment of the patients with acute pancreatitis, it can improve the overall state of pancreas, and has significant therapeutic effect.