Objective To analyze the value of two-dimensional ultrasound diagnosis of medullary thyroid carcinoma.Methods The two-dimensional ultrasonographic manifestations of 18 nodules in 13 medullary thyroid carcinoma patients pathologically confirmed were analyzed retrospectively.Results In 18 nodules of medullary thyroid carcinoma,18 nodules were hypoechoic,13 nodules were irregular shaped,14 nodules had unclear boundaries,15 nodules with an aspect ratio less than 1,13 nodules had calcifications and 9 patients had neck lymphatic enlargement.Conclusion Medullary thyroid carcinoma has some two-dimensional ultrasonographic manifestations:hypoechoic nodules,no obvious envelope with coarse calcification and widely distributed,and tiny calcified nodules of hypoechoic,round,non-calcified with an aspect ratio greater than 1 and the diameter less than 10 mm should be wary of medullary thyroid carcinoma.

Objective: To develop parental punishment style the questionnaire. Methods: Based upon previous literature about parental punishment style and expert interview, data were collected for the parental punishment style questionnaire. A sample of 1028 junior high school students were asked to complete the questionnaire and 158 of them was given retest after a month. Results: Taken from the questionnaire by exploratory factor analysis four factors were compatible. The results of confirmatory factor analysis showed a good fit to the data. The internal consistency reliability of father and mother questionnaire was satisfactory, with Cronbach ? coefficient 0.91; and the test-retest reliability was also satisfactory, with test-retest correlation coefficient 0.88 and 0.89, which showed good content validity and high construct validity. Conclusion: The questionnaire has good reliability and validity, and meets the need of psychometrics.

Objective To study the clinical significance of urinary mtcroprotems examination on early diagnosis of kidney damage of children with obesity. Method Urinary microproteins were tested in 40 obese children by enzyme - linked immunosorbent assay and 50 healthy children as controls. Results Urinary microprotem examination were abnormal in 8 obese children. Albumin in 5 cases increased, and transferrin(TRF) with retinol-binding protein in 1 case increased meanwhile. TRF in 3 cases increased. One case was abnormal by kidney biopsy.Conclusion Urinary microprotem examination was the sensitive index in early diagnosis of obesity-related glomerulopathy in children.

Objective To explore the information distribution of PM2.5 Paper in Foreign Language.Methods This article took PM2.5 papers as research object,used GoPubMed as information search and statistical analysis tool to conduct information mining of PM2.5 literature,including subject,time,author,source,distribution and number of publications over time.Results There was steady development of PM2.5 literature published in foreign language from 2004 to 2012,dramatic increase during the year 2013 to 2015.The three top publishing years of PM2.5 literature were 2015,2014 and 2013.Main authors of such literature include Chow J,Watson J,Koutrakis P and so on.Main research countries include United States,China,Canada,etc.Big cities,like Beijing,Shanghai,Boston were main places conducted such PM2.5 research.Related articles mainly published in journals such as J Air Waste Manag Assoc,Environ Sci Technol,Sci Total Environ and so on.Six out of the top ten research journals have higher impact factors than the average level of all journals of SCI in 2014.Main research terminologies include particulate matter,air pollutants,particle size and so on.Conclusions Generally,the PM2.5 literature over the world has a high level academically and China plays a significant role in such research.

AIM: To select differentially the expressed gene,glucose regulated protein 78(GRP78),to observe the expression of GRP78 in atherosclerosis tissue and to elucidate the mechanism for anti-atherosclerosis of puerarin.METHODS: The model was established by using homocysteinemia in vitro.The vascular endothelial cells were incubated with various concentrations of puerarin(10 -7 mol/L,10 -6 mol/L,10 -5 mol/L) for 48 h.The apoptosis rate was detected by flow cytometry using Annexin/PI-staining.GRP78 gene was amplificated by PCR.These differentially expressed fragments were cloned,selected,confirmed,and sequenced.Digoxigenin-labeled DNA Probe and cholesterol-rich rabit model were prepared,GRP78 mRNA was determined by in situ hybridization in atherosclerotic lesions and normal tissue.RESULTS: Three concentrtions of puerarin significantly inhibited the apoptosis of endothelial cells.The early apoptosis rate was decreased magnificently in a dose dependent manner compared with that of HCY group (P

Objective To probe into the status and development trend of ebola,so as to provide reference for further research.Methods This paper took the ebola literature as the object of study,took GoPubMed as the retrieval and statistical tool,and conducted information analysis of the subjects,time,sources,distribution,authors and literature development trends of ebola research.Results The main subjects of ebola literature involved ebola virus and hemorrhagic fever,ebola.There has been a sharp increase on the literature number of ebola in the recent two years,particularly in 2014.The majority of literature came from the United States,and Britain ranked the second.The sources of ebola literature were mainly the SCI journals published in the United States and British.Conclusions GoPubMed can provide a vivid picture of the current situation and development trend of ebola research.Further improvements are needed both in the quality and quantity of ebola research in China.

Objective To probe into the status and development trend of avian influenza,so as to provide reference for further research.Methods The article took the avian influenza literature as the object of study,took Gopubmed as the retrieval and statistical tool,made information analysis of the subjects,time,sources,distribution,authors and literature development trends of avian influenza research.Results The main subjects of avian influenza literature involved influenza in birds and influenza,human.There has been a sharp increase on the literature number of avian influenza in the recent decade with the most in 2006.The United States accounted for the most literature and China ranked the second place.The sources of avian influenza literature were mainly the SCI journals from the United States and European countries.Conclusions Gopubmed can show a vivid picture of the current situation and development trend of avian influenza research.Further improvements are needed both in the quality and quantity of avian influenza research in China

Diabetic neuropathic pain(DNP)is one of the most common complications in clinical, which influenced patients’ daily functions greatly, without clear mechanisms and effective methods.P2X3 receptors play a pivotal role in the formation, transmission and conduction of pain under neuropathic pain models, associated with peripheral sensory nerve excitability enhancement.This paper focuses on the establishment of DNP models, and the effects of P2X3 receptors in diabetes mellitus.

As a novel branch of combinational chemistry, dynamic combinatorial chemistry (DCC) can be viewed as a technique which combines library synthesis and screening in one pot. By addition of molecular target, ligangds, which show binding affinity or strong interaction with the molecular target, can be amplified an young but rapidly growing branch of combinatorial chemistry, has been widely used in organic chemistry, biochemistry, material fields. Ligands in the library can be amplified, since synthesis of the library is screened by a molecular target. Therefore, these structures could be identified easily. Consequently DCC has been widely used in the lead discovery, material chemistry and other fields. On the basis of the principle and method of DCC, this review emphasizes the three factors of DCC, including molecular targets (bio-enzyme, lectin, nucleic acid, organic molecule, inorganic molecule); reaction (disulphide chemistry, ammoniation reduction reaction, hydrazone chemistry, etc.) and analytical method. Meanwhile, limitation, current situation and future development of DCC were also discussed in this paper.