Objective To study the expression of T cell immunoglobulin mucin-3(Tim-3)in hepatocellular carcinoma(HCC)cell line and its influence on the oncobiological behavior of HCC cells.Methods The expression of Tim-3 mRNA and protein in human normal liver cell line L02 and HCC cell line HepG2 and SMMC7721 was assessed by FQ-PCR and Western blot.The siRNA Tim-3 fragments were designed to silence the Tim-3 gene expression in HepG2 cel1.HepG2 cells were transfected with siRNA by using LipofectamineTM 2000.The expression of Tim-3 protein was detected after transfection by Western blot to screen the effective siR-NA fragment.The proliferation and migration potential of hepG2 cells was evaluated after Tim-3 gene silence by the cell growth curve MTT assay and the wound healing assay.Results Both expressions of Tim-3 mRNA and protein in human HCC cell line HepG2 and SMMC7721 were significantly higher than those in normal liver cell line L02(P<0.05).After siRNA transfection,the protein expression of Tim-3 in experimental group was significantly lower than that of the control group.Compared with control group,the proliferative activity and the migration ability were decreased significantly.Conclusion Expressions of Tim-3 mRNA and protein are increased in HCC cell line.Tim-3 expression promotes HCC cell proliferation and migration.

60,treated with high dose rmhTNF and low quantity of pleural effusion)present fine clinical effect.

Objective To investigate the effect of early incision of botulinum toxin type A (A Botox) on the recovery of the incision in the small area of the opposite side of the incision.Methods Totally 300 cases of facial small area scar surgery were randomly divided into three groups according to the random number table,with 100 cases in each group.The pations of normal group according to the conventional method,the facial scar surgery,after the surgery,clean cut,did not do special treatment.The pations of control group were treated according to the conventional method for facial scar surgery,postoperative cleaning of incision,from postoperative to postoperative 3 months reduction treatment,were treated with 3M free seam tape.The pations of observation group in the same way for facial scar surgery,a local injection of Botox and from postoperative to postoperative 3 months reduction treatnent,were treated with 3M free seam tape.After 3 months and 1 years of follow-up,the condition of scar recovery were evaluated.After 1 years of follow-up,the treatment efficiency was compared.Results Treatment for 3 months,the VSS,OSAS,and PSAS of observation group were 5.2 ± 1.4,14.7 ± 2.4,15.4 ±3.6,were lower than those of the control group and those of the normal group (P ＜ 0.05).One year after treatment,the score of VSS,OSAS,and PSAS of three groups were reduced.Those of observation group were lower than those in the control group and those in the normal group (P ＜ 0.05).The cure rate of observation group was 82％,which was significantly higher than that of the control group 63％and that of the normal group 52％ (P ＜ 0.05).The total effective rate of observation group was 97％,was significantly higher than that of the control group 85％ and that of the normal group 79％ (P ＜ 0.05).Conclusion The method of BotoxA early incision injection combined with the reduction method can effectively promote the small area of facial scar surgery incision recovery effect,improve the cure rate,to meet the needs of patients with facial beauty.

4 mouse hybridoma cell lines stea-dily secreting monoclonal antibodiesagainst surfaced antigens of mouseleukaemia cells were obtained byfusing mouse SP2/0 myeloma cellswith SW-1 mouse spleen cells im-mnized with viable leukaemia asci-tic clone-1(LAC-1)cells,The McAbwas found especially to bind onlywith LAC-1 cells but not with nor-mal SW-1 mouse thymocytes andConA stimulated thymocytes orot her mouse tumor cells includingL_(6565),L_(783),SRS,L_(7712)mouseleukaemia as well as the leukaemiavirus isolated from LAC-1 cellculture supernatant by ELISA.The McAb lost its binding abilityafter absorption with LAC-1 cells.All of the McAbs were of IgG2b sub-classes and did not show any cytoto-xicity to LAC-1 cells in the presenceof complement in vitro,but theydid block the specific cytotoxicityto the targets affected by LAC-1cells induced Tc in ~(51)Cr releaseassay.

To observe the therapeutic effects of catgut implantation at Weiwanxiashu (Ex-B 3) in the treatment of diabetes, catgut implantation was used to treat 62 cases of diabetes. In group A, 30 cases were treated just by catgut implantation; in group B, 32 cases were treated by catgut implantation and western medicine; in group C, 30 cases were treated just by western medicine. After treatment, the effective rate was 90.0% in group A, 93.7% in group B and 60.0% in group C. The therapeutic effects in groups A and B were obviously better than that in group C (P＜0.01). These are of great significance for diabetic patients in controlling blood sugar and preventing and treating complications.

Craniocerebral Trauma ; surgery ; Humans ; Maxillofacial Injuries ; surgery ; Reconstructive Surgical Procedures ; methods

Child ; Complement C3 ; metabolism ; Cytokinins ; analysis ; metabolism ; Female ; Humans ; Immunoglobulin A ; immunology ; Immunoglobulin G ; immunology ; Interferon-gamma ; immunology ; Interleukin-12 ; analysis ; immunology ; Interleukin-2 ; analysis ; immunology ; Male ; Peptide Fragments ; immunology ; Respiratory Tract Infections ; epidemiology ; immunology ; virology ; Secondary Prevention ; Tuberculin ; analysis

Colorimetry ; methods ; Humans ; Manganese ; urine ; Potassium Permanganate

Objective To investigate the mechanism of liver X receptor(LXR)signal pathway in regulating glucose metabolism by observing the variety of LXR expression and its impacts on regulating the mRNA expression of PEPCK and GCK, the key enzyme of hepatic glucose metabolism in various glucose metabolic status in rats. Methods SD rats were chosen and divided into four groups:the CON group, the induced DM group, the OB group, and the induced OB+DM group. For each group of rats, body weight, blood glucose, serum triglycerides, and cholesterol were measured. Then the rats were sacrificed and the livers were collected and studied. Real-time PGR was used to measure the expressions of LXR mRNA, PEPCK mRNA, and GCK mRNA in the livers. Finally, the Western Blot assay was used to measure the liver LXR protein expression. Results The expression of LXR mRNA was significantly higher in DM,OB, and OB+DM groups than in CON group(P＜0.05).The Western blot results showed that the levels of protein were in accordance with the mRNA expression. Comparing to the CON and the OB groups, the PEPCK mRNA expression of the OB+DM and the DM groups was significantly higher, while the GCK mRNA expression of these two groups was significantly lower(P＜0.05). Comparing to the CON group, the PEPCK mRNA expression of the OB group was significantly lower, while the GCK mRNA expression of OB group was significantly higher(P＜0.05).Conclusions During non-diabetic phase, LXR could act as a protective receptor for glucose metabolism and keep glucose homeostasis by regulating the key enzymes of the hepatic glucose metabolism. While in the diabetic phase, the protective receptor LXR failed to reverse the change of the related enzymes caused by insulin deficiency, and finally the plasma glucose level was raised.

The source, super-antioxidant activity, biological safety and application in medicine, health food and animal feed industry of natural astaxanthin are reviewed in present paper. The industrialization prospect of astaxanthin in China is also proposed.