According to the project of personnel cultivation in 21st century,we have increased interdisciplinary comprehensive experimental courses to intensify the practical education,optimize operating model and teaching method of experimental courses.Experimental education must be strengthened in order to culture students' creativity.

Industrial culture media for cultivation of marine microalgae Nannochloropsis oculata was optimized by using Random Design software. High eicosapentaenoic acid (EPA) content was obtained over 40% of total fatty acid, and high average specific growth rate of 0.32d -1 was obtained in cells grown in the selected Formula 1. Mass cultivation of this species was practiced in indoor open raceway ponds of 6.12 m 2 and 28.02 m 2, and in outdoor open pond of 83.50 m 2 during May and June in 1999. The average area yield of biomass were obtained at 2.77, 1.62 and 2.52 g /m 2?d, and in October, 1.09 g/ m 2?d was obtained in 83.5m 2 outdoor pond, and the harvest algae powder contained 49.50% of protein, 20.30% of total lipid and 3.00% of EPA of dry weight. With high temperature tolerant species during August in 2000, the average area yield of biomass were increased to 5.44?9.96 and 7.66 g/ m 2?d in above ponds, respectively and 2.50% of EPA of dry weight was contained in the powder. It is indicated that the industrialized cultivation of Nannochloropsis oculata rich in EPA is completely doable on practice, and the algal powder was a good raw material as single cell protein and development of EPA rich products.

Adult ; Cerebral Ventricle Neoplasms ; diagnosis ; metabolism ; Diagnosis, Differential ; Glial Fibrillary Acidic Protein ; analysis ; Humans ; Immunohistochemistry ; Lateral Ventricles ; chemistry ; pathology ; Male

Anion Exchange Protein 1, Erythrocyte ; metabolism ; Antiporters ; metabolism ; Child ; Chordoma ; metabolism ; pathology ; radiotherapy ; surgery ; Female ; Follow-Up Studies ; Humans ; Skull Base Neoplasms ; metabolism ; pathology ; radiotherapy ; surgery ; Vimentin ; metabolism

Adult ; Carbodiimides ; poisoning ; Humans ; Male ; Methylamines ; poisoning ; Occupational Exposure ; Young Adult

Chronic Disease ; Forkhead Transcription Factors ; Hepatitis, Viral, Human ; Humans

Animals ; Epithelial Cells ; cytology ; drug effects ; Humans ; Kidney Tubules ; cytology ; drug effects ; Oleanolic Acid ; analogs & derivatives ; pharmacology ; Podocytes ; drug effects

Objective To investigate the function of donor-derived dendritic cells (DCs) treated with NF-?B decoy in prolonging murine cardiac allograft survival time.Methods Donor bone marrow- derived DCs were treated with NF-?B decoy in vitro.Heterotopic abdominal heart transplantation was performed from BALB/c to C57BL/6 mice.Recipients were grouped according to different pretreat ments as follows:(1) Control group,infusion of PBS (0.2 ml) alone intravenously via the portal vein 7 days before heart transplantation;(2) CsA group,treated with sub-therapeutic CsA only for 7 days (10 mg?kg~(-1)?day~(-1)) through intraperitoneal injection after transplantation,and the same as control group before transplantation;(3) Control DCs group,infusion of only cultured 5th-day recipient DCs untreated with NF-?B ODN decoy;(4) Treated DCs group,infusion of recipient DCs pretreated with NF-~cB ODN decoy;(5) Combined treatment group,infusion of recipient DCs treated with NF-~cB ODN decoy before transplantation and intraperitoneal injection of sub-therapeutic CsA for 7 days (10 mg?kg~(-1)?day~(-1)) after transplantation;(6) Third party donor group,C3H/HeJ mice used as donor, and recipient (C57BL/6) was treated the same as combined treatment group.Every group had 8 mice and graft survival time was observed.Cytokines (IL-2,INF-?,IL-4 and IL-10) in recipient serums were analyzed by ELISA at 7th day after transplantation.Results The graft mean survival time (MST) in control group,CsA group,Control DCs group,treated DCs group,combined treatment group and third party donor group was 7 days,10.3 days,7.6 days,21.4 days,53.6 days and 9 days,respectively.There was significant difference in MST between treated DCs group and control group or control DCs group (P

Objective This study is to identify the presence of superoxide anion-generating system in human lens epithelial cells using arachidonic acid (AA) as the stimulator.Design Experimental study.Participants Human lens epithelial cells B3 (HLE B3). Methods Confluent human lens epithelial cells (HLE B3) were subjected to stimulation by AA and its derivatives.The generation of su- peroxide anion was quantified with a luminometer (LumiStar BMG) immediately upon AA and its derivatives addition.Cells preloaded with superoxide dismutase (SOD) or mannitol were used as negative controls and cells mixed with 3% ethanol (solvent for AA) were used as baseline.Cells were preloaded with inhibitors 30 minutes before luminometer measurement.A time-and concentration-depen- dent study on the AA-stimulated activation of mitogen-activated protein kinase (MAPK) was carried out using western blot analysis. Main Outcome Measures Superoxide generation,phosphorylation of MAPK.Results AA at dosage of 30-150 mM proportionally in- duced luminescence in HLE B3 cells,but was ineffective in cells preloaded with SOD or mannitol.DPI,a non-specific NADPH oxidase inhibitor eliminated AA-induced superoxide anion generation partially.Leinoleic acid,stearic acid,eicosa-11Z,14Z,17Z-trienoic acid (20:3) and eicosa-11Z,14Z-dienoic acid (20:2) were ineffective.The generation of superoxide anion was not contributed by cyclooxyge- nase or the cytochrome p450 pathway since indomethacin (inhibitor for cyclooxygenase) or ketoconazole (inhibitor for cytochrome p450) could not eradicate the stimulatory effect of AA.While CDC,a specific inhibitor for lipoxygenase could eliminate superoxide generation partially.The specific inhibitor for 5-lipoxygenase AA861 completely blocked the generation of superoxide anion.Western blot analysis of the cell lysate showed that AA at the concentrations of 30-150 mM progressively activated ERK and JNK.They were transiently ac- tivated between 2.5-30 minutes.The activations of ERK and JNK were dose-dependent and time-dependent.Conclusions Inhibition of superioxide anion generation may be a new approach to block lens epithelial cell proliferation and post-capsule opacification.

False and negative information has brought forth serious impacts on doctor-patient relationship in the new media era as a result of “Broken window effect”and “Butterfly effect”.The underlying reasons are herd mentality,the public’s need to vent their discontent,the subjectivity of information distribution on the Internet and lack of proper supervision and punishment mechanism.Medical Institutions should pay special attention to the new media,leveraging it to improve doctor-patient relationship by making official voice,guiding public opinion,solving problems,and providing comfortable social environment and rational public opinion environment on the Internet.