Objective To compare the treatment effects and side events between chemoradiotherapy and radiotherapy alone for patients with primary cervical cancer.Methods Totally 197 patients with primary cervical cancer in our hospital from 2000 to 2006 were studied.They were divided into two groups: radiotherapy alone(RT)or cisplatin-based chemoradiotherapy(CRT).Group of RT included 100 patients who received external irradiation by ~(60)Co and intracavitary irradiation by ~(192)Ir.Group of CRT included 97 patients who received cisplatin-based chemoradiotherapy.The RT regimen was the same as group RT.Results The local control(CR+PR)in two groups had no statistic difference(P=0.500).The total 5 year survival rate in the two groups were 82% and 79%,with no significant difference(P=0.177);however,there was a significant difference for stage Ⅲ squamous cell carcinoma of the cervix,the 5 year survival rate being 56% and 84%,respectively(P

BACKGROUND:A large number of literatures have shown that surgeons expose to a higher radiation dose during puncture and bone cement injection in percutaneous vertebral augmentation. OBJECTIVE:To review the research progress in radiation doses and safeguard procedures in percutaneous vertebral augmentation. METHODS: By using percutaneous vertebral augmentation, radiation doses, radiation protective as key words, we retrieved articles related to radiological protection during percutaneous vertebral augmentation published from January 1995 to December 2014 in Wangfang database and PubMed database. RESULTS AND CONCLUSION:One basic principle of radiation protection is that the radiation dose decreases rapidly with distance from the radioactive sources. Surgeons should maximize the distance from the radioactive sources as far as possible in case there is no effect on the operation. To optimize the setting and position of C-arm machine, wear protective devices and put lead shields as wel as computer navigation and radiation training in surgeons al can help to reduce the radiation doses during percutaneous vertebral augmentation. Remote bone cement injection device is easy to control but not increases the radiation doses. In addition, the influence of surgery bed on radiation dose remains to be further studied. We believe that with the in-depth research on radiological protection, vertebral augmentation technique wil be safer in clinical application.

Objective To discuss the effect of mesenchymal stem cells (MSCs) in modulating immune responses in a rat renal transplantation model.Methods An in vivo trial of cytology was performed in one centre from March to December in 2008.Wistar rat donors and Lewis rat recipients in a renal transplantation model were randomly divided into 4 groups:MSCs (low dose,1 × 106 )therapy,MSCs (high dose,1 × 107) therapy,CsA monotherapy,and no therapy.Biochemistry methods were used to detect the levels of creatinine in serum.The survival time,renal grafting function and pathological changes of transplanted renal tissues were observed.Results Animal survival was significantly prolonged by MSCs (high dose) therapy and CsA monotherapy as compared with the no therapy group (both P＜0.01).Animal survival in the MSCs (low dose) therapy group was prolonged as compared with no therapy group (P＜0.01),but shortened as compared with MSCs (high dose) therapy group (P＜0.05) and CsA monotherapy group (P＜0.05).The MSCs (high dose) therapy and CsA therapy groups showed no special changes in histology,hut the control group showed acute rejection.Conclusion MSCs down-regulated immune responses,reduced production of some inflammatory mediators,preserved graft function in the initial stage after transplantation,and prolonged animal survival,and these effects were the same as those of CsA therapy with 1 × 107/day.

Objective To study the expression and clinical significance of Survivin and PTEN in cervical carcinoma. Methods The expression of Survivin and PTEN in 60 cases of cervical carcinoma, 15 cases of CIN Ⅰ , 15 cases of CIN Ⅱ, 15 cases of CIN Ⅲ and 15 cases of normal cervical tissues were detected by immunohistochemical staining. Results The expression positive rates of Survivin were 86.67 %, 80.00 %,33.33 %, 20.00 % and 0, respectively, and those of PTEN were 21.67 %, 40.00 %, 80.00 %, 86.67 % and 100.00 % in cervical carcinoma, CIN Ⅲ, CIN Ⅱ, CIN Ⅰ and normal cervical tissues, respectively. The expression positive rates of Survivin were increased along with normal cervical epithelium, CIN Ⅰ, CIN Ⅱ,CIN Ⅲ and invasive carcinoma of cervix;while those of PTEN were inverse order.Compared with the expression positive rates of Survivin and PTEN protein in the CIN Ⅱ group, those in the CIN Ⅲ group had significant differences (P <0.01 and P <0.05, respectively). Expressions of Survivin and PTEN were correlated to the size of focus(P <0.05), the depth of tumor and pelvic lymphnode metastasis (P <0.05), but not to clinical staging, pathological types, pathological grading and age(P >0.05). There was a negative correlation between the expression of PTEN and Survivin in cervical carcinoma. Conclusion The expressions of Survivin and PTEN are correlated with invasion and metastasis of cervical carcinoma, and be related to clinical pathotology.Survivin and PTEN may play a important role in the formation, proliferation, differentuation and metastasis of cervical carcinoma. They may be used as markers of early diagnose, efficacy and prognosis evaluation.

Gastric cancer is one of the most common malignant tumors in China, and its pathogenesis is complex. At present, there is no good method for the diagnosis and treatment of gastric cancer. However, studies have shown that microRNA (miRNA) has abnormal expression in gastric cancer, which participates in the regulation of gastric cancer related genes, and has an impact on the occurrence, development, diagnosis and treatment of gastric cancer. This paper aims to review the relationship between miRNA and the diagnosis and treatment of gastric cancer, as well as the drug resistance in the treatment, so as to lay the foundation for the follow-up research and clinical diagnosis and treatment.

Ten glucosyloxybenzyl 2-isobutylmalates and one benzyl alcohol glycoside were isolated from the dry tuber of Pleione bulbocodioides, which is a specie of Orchidaceae family and its dry tuber is one of the main sources of traditional Chinese medicine "shanci-gu", by a combination of various column chromatographic methods, including ODS, macroporous adsorbent resin, Sepheadex LH-20, and preparative HPLC. Their structures were identified on the basis of chemical evidences and spectroscopic analysis asloroglossin (1), grammatophylloside A (2), cronupapine (3), (-)-(2R, 3S)-1-(4-β-D-glucopyranosyloxybenzyl)-4-methyl-2-isobutyltartrate (4), vandateroside II (5), grammatophylloside B (6), bis [4-(β-D-glucopyranosyloxy) -benzyl] (S) -2-isopropylmalate (7), gymnoside I (8), militarine (9), dactylorhin A (10), gastrodin (11). Compounds 1-7 were isolated from this genus for the firt time.
Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Glucosides ; chemistry ; isolation & purification ; Malates ; chemistry ; isolation & purification ; Molecular Structure ; Orchidaceae ; chemistry ; Spectrometry, Mass, Electrospray Ionization

Objective To analyze the clinicaopathological features and treatment of bilateral asynchronous breast cancer.Methods The clinical and pathological data of 13 patients with bilateral asynchronous breast cancer admitted in our hospital from 2010 to 2016 ere retrospectively reviewed.Results The bilateral asynchronous breast cancer accounted for 1.7% of all breast cancer patients admitted n the same period (13/782).The median age of patients at first onset was 47.The modified radical operation was the main surgical lity (12/13 for the first cancer;8/13 for the second cancer).The clinical stage Ⅱ was predominant in all patients (10/13 of the first r;9/13 of the second cancer).Invasive ductal cancer was the most common pathologic type (in 11/13 of the first cancer;11/13 of the cond cancer).Hormone receptors were highly expressed in most of the cases (9/13 of the first cancer;7/13 of the second cancer).The disease-free interval was more than 10 years in most cases (8/13).Among 13 cases local recurrence was detected in 1 case, distant metastases in 2 cases, and 1 case died from distant metastasis.Conclusion The bilateral asynchronous breast cancer has a low incidence;however, a close follow up is necessary for early detection and treatment of the second carcinoma.

Objective:This experiment aims to find a appropriate scaffold for Tissue Engineer- ing.Methods:The chondrocytes were cultured when they were seeded onto PGA+PLA scaffolds coat- ing with Lecithin(LEC)and Poly-l-lysine(PLYS)together and respectively.With light microscope and scanning electron microscope,the observation of the scaffolds in hydrophilia and adsorptivity to chondrocytes and the function of the cells was made.Results:The PGA scaffolds coating with LEC and PLYS have better hydrophilia and adsorptivity to the cells;on which the chodrocytes produce more ma- trices.Conclusion:LEC can chang the hydrophilia of the scaffolds;while PLYS can strengthen the ad- sorptivity of the scaffolds;the PGA coating with LEC and PLYS is an ideal scaffold in Tissue Engineer- ing.

BACKGROUND: Some studies in vitro have reported that there are CD30 positive T cells in immunological response of allogenic transplantation.OBJECTIVE: To detect the relationship between the level of serum CD30 (sCD30) and clinical rejection in the patients with or without kidney transplantation, and analyze the importance of sCD30 in the estimation of immune state, monitor of acute rejection, and judgment of prognosis. DESIGN, TIME AND SETTING: Clinical case analysis study was performed at Jiangsu People's Hospital between April 2004 and March 2007. PARTICIPANTS: 153 kidney transplantation cases comprising 103 males and 50 females, averagely aged 37 years. METHODS: 3 mL peripheral blood was obtained from recipients before transplantation (without immunosuppressive agent) and at 0, 1, 3, 5, 7, 14, 21, and 28 days. Serum was isolated from obtained blood and placed at -20 ℃. Soluble CD30 levels were detected using CD30 cytokine ELISA kit supplied by BenderMedSystems. MAIN OUTCOME MEASURE: The relation between the soluble CD30 levels and rejection prior to and following transplantation.RESULTS: There was a significant relation in the sCD30 level between the patients with (n=17) and without acute rejection (n=136). The CD30 levels were 113.2 U/mL in the rejection group and 83.2 U/mL in the non-injection group (P < 0.01). No significant difference was determined between both groups in 5 days following surgery (P > 0.05). Significant difference were detected between both groups from 5 days following surgery (P < 0.01). There was no relation between the soluble CD30 level and the time of rejection and release after kidney transplantation (P > 0.05). Receiver operating characteristic (ROC) curve demonstrated that soluble CD30 levels on day 5 post-transplantation could predict acute rejection (area under ROC curve: 0.850). Meanwhile, 100 U/mL was the optimal operational cut-off level to predict rejection (specificity: 85.0%; sensitivity: 83.6%). The patients with positive of soluble CD30 level showed a lower survival rate than those with negative CD30 level (P < 0.01). CONCLUSION: The soluble CD30 levels contributed to predictive the acute rejection and prognosis of kidney transplantation.

Fourteen compoumds were isolated from the ethyl acetate portion of the 95% ethanolic extract of Pleione bulbocodioides by a combination of various chromatographic techniques including silica gel, ODS, macroporous adsorbent resin, Sephadex LH-20, and preparative HPLC, of which ten compoumds were phenanthrenes and dihydrophenanthrenes, two compoumds were bibenzyls, one was lignan and a sterol. Their structures were identified on the basis of spectroscopic data as monbarbatain A(1), 2, 7, 2'-trihy-droxy-4, 4', 7'-trimethoxy-1, 1'- biphenanthrene(2), blestriarene A(3), pleionesin B(4), shanciol H(5), 17-hydroxy-7'-(4'-hy-droxy-3 '-methoxyphenyl)- 4-methoxy-9, 10, 7', 8'-tetrahydrophenanthro[2, 3-b]furan-8'-yl methyl acetate(6), 1-p-hydroxybenzyl-4-methoxy phenanthrene-2, 7-diol(7), 1-p-hydroxybenzyl-4-met-hoxy-9, 10-dihydrophenanthrene-2, 7-diol(8), hircinol(9), coelonin( 10), gigantol(11), batatasin 11 (12), syringaresinol(13) and ergosta4, 6, 8 ( 14) , 22-tetraen-3-one (14). Compounds 1-3, 9, 13 and 14 were isolated from this genus for the first time.
Drugs, Chinese Herbal ; chemistry ; Orchidaceae ; chemistry ; Organic Chemicals ; analysis