Objective To evaluate the role of PI3K-Akt-HIF-1α signaling pathway in dexmedetomidine-induced reduction of damage to the unventilated lung during one-lung ventilation (OLV) in rats.Methods Forty adult male Sprague-Dawley rats,weighing 250-350 g,were randomly divided into 4 groups (n=10 each) using a random number table:two-lung ventilation TLV) group,OLV + normal saline group (group OLV),OLV + dexmedetomidine group (group D),and OLV + dexmedetomidine +LY294002 group (group DL).Bilateral lungs were ventilated for 2.5 h in group TLV,and the right lung was ventilated for 2 h followed by 0.5 h TLV in the other groups.The rats were sacrificed,the left lung was removed for determination of wet to dry lung weight ratio (W/D ratio),and expression of phosphorylated Akt (p-Akt) and hypoxia-inducible factor-1 alpha (HIF-1α) (by Western blot).Results Compared with group TLV,W/D ratio was significantly increased,the expression of p-Akt was down-regulated,and the expression of HIF-1α was up-regulated in the other three groups (P＜0.05).Compared with group OLV,W/D ratio was significantly decreased,and the expression of p-Akt and HIF-lα was up-regulated in D and DL groups (P＜0.05).Compared with group DL,W/D ratio was significantly decreased,and the expression of p-Akt and HIF-1α was up-regulated in group D (P＜0.05).Conclusion PI3K-Akt-HIF-1α signaling pathway is involved in dexmedetomidine-induced reduction of damage to the unventilated lung during OLV in rats.

Improved medical postgraduate reexamination system is essential to the quality of students. In this study, organization of medical postgraduate reexamination was discussed from the perspectives of preliminary arrangements of medical postgraduate reexamination, fairness of reexami-nation process and improvement of the follow-up after the reexamination. Sufficient attention on the re-examination, effective supervision of the reexamination process and control of human factors were the three main factors for the successful medical postgraduate reexamination and effective guarantee for the improvement of postgraduate quality.

Objective To observe the clinical effect of long-range treatment for elderly patients with reflux esophagitis.Methods 124 reflux esophagitis patients were randomly divided into observation group (62 cases) and control group (62 cases) according to random number method.The observation group received long-range treatment (maintain treatment for 3 months after the end of the routine treatment),while the control group received conventional treatment,symptoms score and endoscopic healing of two groups were compared.Results Before treatment and 3 months after treatment,two groups of reflux symptom scores were not significantly different (t =1.325,1.241,all P ＞ 0.05),6 and 12 months after treatment,reflux symptoms score of the observation group was significantly lower than that of the control group (t =24.325,18.124,P ＜ 0.01),after total treatment,the good treatment effect rate of the observation group was 90.0%,which was higher than that of the contol group(55.7%)(x2 =17.902,P ＜0.01),healing of esophagitis excellent rate of the observation group was 86.7%,which was higher than that of the contol group (57.4%) (x2 =12.844,P ＜0.01).Conclusion Long-range treatment of symptomatic improvement has good effect in elderly the reflux esophagitis,the lesion healing to improve the quality,which is worthy of further promotion research.

Objective To observe the change of Visual Analogue Scale (VAS) and satisfaction with treatment in preventing remissive migraine by puncturing Taiyang (EX-HN 5) at different depths. Methods Forty eligible migraine patients were randomized into a treatment group and a control group, 20 in each group. The treatment group was intervened by deep needling at Taiyang (EX-HN 5) as the predominant treatment, while the control group was by superficial needling at Taiyang (EX-HN 5) as the predominant treatment. The VAS score and satisfaction degree with treatment were evaluated before and after treatment. Results The VAS score was significantly changed after intervention in the treatment group (P<0.01). After intervention, there was a significant difference in comparing the VAS score between the two groups (P<0.01). The satisfaction rate was 100.0% in the treatment group versus 85.0% in the control group, and the difference was statistically insignificant (P>0.05). Conclusions Compared to superficial needling at Taiyang (EX-HN 5), deep needling at Taiyang (EX-HN 5) can significantly reduce the headache degree..

Objective To discuss the medication rules of Glycyrrhizae Radix et Rhizoma in Shang Han Lun based on dose-response relationship;To provide references for clinical application. Methods It systematically arranged the provisions containing Glycyrrhizae Radix et Rhizoma in Shang Han Lun. It statistically analyzed the relationship among Glycyrrhizae Radix et Rhizoma and other various factors by using SPSS 20.0. Results After screening, 70 prescriptions were included, among which 2 (2.86%) contained raw Glycyrrhizae Radix et Rhizoma and 68 (97.14%) contained Glycyrrhizae Radix et Rhizoma Praeparata cum Melia. The taken dose of every time had a close relationship with the number of medicine, water consumption, residual water, water consumption per time and frequency of use, which had a statistical significance (P<0.01). Univariate logistic regression analysis showed whether Glycyrrhizae Radix et Rhizoma as a monarch medicine had the close relationship with the number of medicine (P=0.029). In 48(73.85%) prescriptions contained Glycyrrhizae Radix et Rhizoma Praeparata cum Melia as non-monarch medicine, the taken dose of every time and the number of drugs had statistical differences with that in the prescriptions which as monarch medicine (P=0.000). When Glycyrrhizae Radix et Rhizoma Praeparata cum Melia was in compatibility with other medicine, its dose had a certain rules with the number of medicine. When it was the basic medicine, there was no compatibility with Zingiberis Rhizoma Recens, Jujubae Fructus, and Ginseng Radix et Rhizoma. Conclusion It can be found from the dose-response relationship that Glycyrrhizae Radix et Rhizoma has a widely dose and combination efficacy in different prescriptions. Its flexibility, diversity and scientific nature provide the solid basis for clinical application.

Objective To observe the effect of acupuncture at Back-shu points on chronic fatigue syndrome (CFS).Methods The 45 CFS patients were randomized into two groups. Acupuncture at Back-shu points was used in the treatment group (22 cases), while placebo needling was given to the control group (23 cases). SF-20 and Chalder fatigue scale were used to measure the degree of general health before and after treatment.Results In the treatment group, the scores of SF-20 in physiological functions, social functions, role functions, psychological health, overall health, and pain were significantly increased(P

This paper analyzes the causes of graduates′ breaking contracts in medical colleges.Then it points out some countermeasures such as career guidance and medical ethics education.

OBJECTIVE:To review the current situation and the trend of development of oral hypoglycemic agents in our hospital.METHODS:The defined daily dose DDDs and the sales amount of oral hypoglycemic agents used in our hospital during2001～July,2004were analyzed statistically.RESULTS:The DDDs of sulfonylureas was decreasing,whereas it still accounted for more than70%of the total DDDs;the glipizide ranked the first in DDDs during these years;the DDDs of biguanides kept increasing year by year;the sales amount of?-glucosidase inhibitor and thiazolidine diketone drugs was in?creasing;all the‘Medical Insurance’drugs accounted for more than97%of the total DDDs.CONCLUSION:The oral hy?poglycemic agents used in our hospital is rational on the whole.

Objective:To evaluate the short-term and middle-term therapeutic effects and the safety of deep acupuncture at Tianshu(ST 25) for treatment of slow transit constipation(STC).Methods:60 cases of STC were randomly divided into an acupuncture group and a control group.Cleveland Constipation Score(CCS) and colic transit time(CTT) before treatment,2 weeks and 6 months after the treatment were investigated,and the safety was evaluated by clinical,laboratory and imaging indexes.Results:For the short-term therapeutic effect,the treatment group was better than the control group(P

Objective To evaluate the clinical efficacy of Danhong injection combined with edaravone in the treatment of acute ischemic stroke.Methods 90 patients with acute ischemic stroke were randomly divided into control group and observation group,45 cases in each group.The control group was given edaravone treatment on the basis of conventional treatment,the observation group was treated with Danhong injection combined with edaravone treatment,the two groups were treated for 14 days.The changes of main physiological indices,clinical efficacy and adverse reactions etc.were observed.Results After treatment,in the two groups,the differences were statistically significant in the platelet aggregation and plasma viscosity compared with before treatment (t =4.412,4.215,3.984,4.608,all P ＜ 0.05),and the differences were statistically significant in the improvement of the observation group compared with the control group(t =5.553,4.436,all P ＜ 0.05).The total effective rate of the observation group was 88.9％,that of the control group was 68.9％,the difference was statistically significant between the two groups (x2 =2.702,P ＜0.05).The incidence of adverse reactions in the observation group was 15.6％,which in the control group was 17.8％,the difference was not statistically significant between the two groups (x2 =0.040,P ＞ 0.05).Conclusion On the basis of conventional therapy,Danhong injection combined with edaravone in the treatment of acute ischemic stroke can significantly improve the clinical symptoms of the patients,improve the clinical efficacy and has good safety and great clinical significance.