Objective To evaluate the role of PI3K-Akt-HIF-1α signaling pathway in dexmedetomidine-induced reduction of damage to the unventilated lung during one-lung ventilation (OLV) in rats.Methods Forty adult male Sprague-Dawley rats,weighing 250-350 g,were randomly divided into 4 groups (n=10 each) using a random number table:two-lung ventilation TLV) group,OLV + normal saline group (group OLV),OLV + dexmedetomidine group (group D),and OLV + dexmedetomidine +LY294002 group (group DL).Bilateral lungs were ventilated for 2.5 h in group TLV,and the right lung was ventilated for 2 h followed by 0.5 h TLV in the other groups.The rats were sacrificed,the left lung was removed for determination of wet to dry lung weight ratio (W/D ratio),and expression of phosphorylated Akt (p-Akt) and hypoxia-inducible factor-1 alpha (HIF-1α) (by Western blot).Results Compared with group TLV,W/D ratio was significantly increased,the expression of p-Akt was down-regulated,and the expression of HIF-1α was up-regulated in the other three groups (P＜0.05).Compared with group OLV,W/D ratio was significantly decreased,and the expression of p-Akt and HIF-lα was up-regulated in D and DL groups (P＜0.05).Compared with group DL,W/D ratio was significantly decreased,and the expression of p-Akt and HIF-1α was up-regulated in group D (P＜0.05).Conclusion PI3K-Akt-HIF-1α signaling pathway is involved in dexmedetomidine-induced reduction of damage to the unventilated lung during OLV in rats.

Objective:To analyze the expression pattern and clinical significance of PBK /TOPK in cervical cancer.Methods:50 cases of low-grade cervical intraepithelial neoplasia ( CINI ) , 45 cases of cervical cancer and 40 cases of high-grade cervical intraepithelial neoplasia collected by pathology department during January 2012 to December 2014 in our hospital were selected ,and the expression of PBK/TOPK was detected by immunohistochemistry .The correlation between PBK/TOPK level and pathological characteristics were compared by statistical methods .Results: PBK/TOPK was expressed in 75.6% cervical cancer samples , much higher than that of 16.7%in non cervical cancer samples;the score of IS on CINI organizations was lower than that in the high-grade cervical intraepithelial neoplasia and cervical cancer ( P<0.05 ,P<0.01 ) ,and the score of IS in the high-grade cervical intraepithelial neoplasia was significantly lower than cervical cancer ( P<0.01 );the expression of PBK/TOPK and P53 in cervical cancer tissues were positively correlated by the non-parametric Spearman rank ( r=0.68 ,P=0.008 );the expression level of PBK/TOPK in cervical cancer significantly correlated with TNM stage,invasion and lymph node metastasis(P<0.05),but not with age and differentiation (P>0.05). Conclusion:PBK/TOPK expression was correlated with cervical cancer ,and it may be served as a molecular marker for clinical tumor diagnose and theapy .

Objective To explore whether microsatellite instability (MSI) is exsist in human gastric carcinoma and precancerous lesion.Methods The MSI was detected in gastric carcinoma and precancerous lesion by tissue laser capture microdissection and denaturing high performance liquid chromatogragh (DHPLC).Results MSI was found in 10.5 ％ intestinal metaplasia,21.12 ％ gastric hyperplasia and 43.75 ％gastric carcinoma respectively.In contrast,there was no microsatellite instability in normol gastric mucosa.It displayed accumulation and alteration from normal to cancerous tissues.Conclusion Pure tumor samples are obtained from tissue laser capture microdissection which could avoid the heteroplasmy of tissue.DHPLC is a high sensitive,fast and semiautomated method for identifying MSI.This experiment showed an increasing tendency of MSI incidence from precancerous lesion to gastric carcinoma.MSI could be used as a marker to predicate the occurrence of gastric carcinoma.

Improved medical postgraduate reexamination system is essential to the quality of students. In this study, organization of medical postgraduate reexamination was discussed from the perspectives of preliminary arrangements of medical postgraduate reexamination, fairness of reexami-nation process and improvement of the follow-up after the reexamination. Sufficient attention on the re-examination, effective supervision of the reexamination process and control of human factors were the three main factors for the successful medical postgraduate reexamination and effective guarantee for the improvement of postgraduate quality.

Objective To observe the clinical effect of long-range treatment for elderly patients with reflux esophagitis.Methods 124 reflux esophagitis patients were randomly divided into observation group (62 cases) and control group (62 cases) according to random number method.The observation group received long-range treatment (maintain treatment for 3 months after the end of the routine treatment),while the control group received conventional treatment,symptoms score and endoscopic healing of two groups were compared.Results Before treatment and 3 months after treatment,two groups of reflux symptom scores were not significantly different (t =1.325,1.241,all P ＞ 0.05),6 and 12 months after treatment,reflux symptoms score of the observation group was significantly lower than that of the control group (t =24.325,18.124,P ＜ 0.01),after total treatment,the good treatment effect rate of the observation group was 90.0%,which was higher than that of the contol group(55.7%)(x2 =17.902,P ＜0.01),healing of esophagitis excellent rate of the observation group was 86.7%,which was higher than that of the contol group (57.4%) (x2 =12.844,P ＜0.01).Conclusion Long-range treatment of symptomatic improvement has good effect in elderly the reflux esophagitis,the lesion healing to improve the quality,which is worthy of further promotion research.

Objective To observe the change of Visual Analogue Scale (VAS) and satisfaction with treatment in preventing remissive migraine by puncturing Taiyang (EX-HN 5) at different depths. Methods Forty eligible migraine patients were randomized into a treatment group and a control group, 20 in each group. The treatment group was intervened by deep needling at Taiyang (EX-HN 5) as the predominant treatment, while the control group was by superficial needling at Taiyang (EX-HN 5) as the predominant treatment. The VAS score and satisfaction degree with treatment were evaluated before and after treatment. Results The VAS score was significantly changed after intervention in the treatment group (P<0.01). After intervention, there was a significant difference in comparing the VAS score between the two groups (P<0.01). The satisfaction rate was 100.0% in the treatment group versus 85.0% in the control group, and the difference was statistically insignificant (P>0.05). Conclusions Compared to superficial needling at Taiyang (EX-HN 5), deep needling at Taiyang (EX-HN 5) can significantly reduce the headache degree..

This paper briefly reviewed the history and current development of traditional Chinese medicine(TCM)in the United Kingdom and introduced TCM associations and TCM colleges and universities in detail in this country.This paper provided a reference for comprehensively understanding the current TCM associations.education and researching institutes in the United Kingdom.

Objective To investigate the clinical characteristics and the treatment of the infection of heli-eobacter pylori in gastric stump. Methods The clinical data of 210 patients who were examined by gastroscopy for gastric stump and 200 randomly selected patients who had no history of operation due to gastrointestinal diseases and received gastroscopy and Hp detections were retrospectively analyzed. Results The Hp infective rate in gastric stump was 26.2 %, and the Hp infective rates had no significant difference among different operative causes. The Hp positive rate after Billroth-Ⅰ operation was obviously higher than that after Billroth-Ⅱ type operation;the Hp posi-tive rate in the patients with bile regurgitation was lower than that in the patients without bile regurgitation. The Hp infective rate became lower as the time after subtotal gastrectomy prolonged. The clearance rate of anti-Hp treatment was 87.3 %. Conclusion The Hp infective rate in gastric stump is lower, but the Hp lesion in gastric stump ks ag-gravated. The anti-Hp therapeutic program which is commonly used at present plus gastric mucosa protectant and gastric motive power drugs are suitable for treating Hp infection in gastric stump, and have definite value in clinical application.

Objective To summarize the experiences of logistics support in disaster areas stricken by the catastrophic Wenchuan earthquake with the aim to improve the ability to effectively meet various military missions. Methods The experiences of logistics support in disaster areas stricken by the catastrophic Wenchuan earthquake were reviewed and analyzed retrospectively. With the author's knowledge of commanding the health service in rescue work, a proposal was put forward on the organization of military logistics and health support to effectively meet various military missions in the future. Results The disaster areas stricken by the strong Wenchuan earthquake were about 500 thousand km2, in which about 130 thousand km2 areas were heavily stricken, and about 4.3 million casualties needed to be treated. The peculiarities of the rescue support in the disaster areas were as follows: urgency in mission, difficulty in coordination, urgent and rapid changes in tasks, and difficelty in ensuring supply. For the military logistic service, the specific services in disaster areas were: to strictly carry out the commands from high authorities and determination to carry out the rescue work of health support; to unify the commands, and coordinate as a whole to enhance the efficiency of health service command; to carry out rational deployment of medical teams and rapidly organize a combined military and civilian medical support system; to dexterously utilize every single resource of health service, and continuously enhance the efficiency of health service. By means of high efficiency of health service, the fighting capacity of troops in carrying out the task had been withheld and enhanced, ensuring timely treatment of the injured, and no outbreak of epidemic, which used to prevail after a strong earthquake. Conclusions In view of existing problems in medical support in rescuing the casualties in this disaster, such as insufficient preparedness of health service in dealing with catastrophic natural disaster, imperfect organization and coordination system, and backward in equipments and techniques, and the prospects that the necessity of developing military health service to meet the needs of various military tasks in the future, it is suggested that: the capacity to respond to meet sudden accidents should be honed, capacity of coordinated action in carrying out tasks by combined forces should be enhanced; the levels of surgical expertise and medical support should be elevated, and the construction of logistics forces should be strengthened.

Objective To explore the potential of bone marrow mesenchymal stem cells(MSCs) to be modified by neurotrophin-3(NT-3) gene and pretreated with retinoic acid(RA) to differentiate into neuron-like cells in the transplanted site of the spinal cord injury. Methods MSCs,RA-induced MSCs,LacZ gene modified MSCs,NT-3 gene modified MSCs,and MSCs both modified by NT-3 gene and pretreated with RA were immediately transplanted respectively into the completely transected site(T_(10) spinal segment) of spinal cord.On the 67th day after the operation,the spinal cord segment was removed and frozenly sectioned.The differentiation potential of MSCs was examined by immunofluorescence histochemistry and the percentage was calculated of neuron-like cells that were differentiated from MSCs among all the transplanted cells groups. Results Transplanted MSCs could differentiate into neural stem cells(nestin-positive), neuroglial cells(GFAP-positive) and neuron-like cells(NF and MAP2-positive) in the injured spinal cord.Some of them also differentiated into the neuronlike cells which contained some neurotransmitters(ChAT and 5-HT positive) or had the potential to form a synapse(PSD95-positive).The percentage of neuron-like cells differentiated from MSCs modified by NT-3 gene and pretreated with RA was the highest among all the transplanted cell groups.Conclusion MSCs modified by NT-3 gene and pretreated with RA could better to differentiate into neuron-like cells in the injured spinal cord.