Objective To evaluate the role of PI3K-Akt-HIF-1α signaling pathway in dexmedetomidine-induced reduction of damage to the unventilated lung during one-lung ventilation (OLV) in rats.Methods Forty adult male Sprague-Dawley rats,weighing 250-350 g,were randomly divided into 4 groups (n=10 each) using a random number table:two-lung ventilation TLV) group,OLV + normal saline group (group OLV),OLV + dexmedetomidine group (group D),and OLV + dexmedetomidine +LY294002 group (group DL).Bilateral lungs were ventilated for 2.5 h in group TLV,and the right lung was ventilated for 2 h followed by 0.5 h TLV in the other groups.The rats were sacrificed,the left lung was removed for determination of wet to dry lung weight ratio (W/D ratio),and expression of phosphorylated Akt (p-Akt) and hypoxia-inducible factor-1 alpha (HIF-1α) (by Western blot).Results Compared with group TLV,W/D ratio was significantly increased,the expression of p-Akt was down-regulated,and the expression of HIF-1α was up-regulated in the other three groups (P＜0.05).Compared with group OLV,W/D ratio was significantly decreased,and the expression of p-Akt and HIF-lα was up-regulated in D and DL groups (P＜0.05).Compared with group DL,W/D ratio was significantly decreased,and the expression of p-Akt and HIF-1α was up-regulated in group D (P＜0.05).Conclusion PI3K-Akt-HIF-1α signaling pathway is involved in dexmedetomidine-induced reduction of damage to the unventilated lung during OLV in rats.

Objective To evaluate the clinical efficacy of Danhong injection combined with edaravone in the treatment of acute ischemic stroke.Methods 90 patients with acute ischemic stroke were randomly divided into control group and observation group,45 cases in each group.The control group was given edaravone treatment on the basis of conventional treatment,the observation group was treated with Danhong injection combined with edaravone treatment,the two groups were treated for 14 days.The changes of main physiological indices,clinical efficacy and adverse reactions etc.were observed.Results After treatment,in the two groups,the differences were statistically significant in the platelet aggregation and plasma viscosity compared with before treatment (t =4.412,4.215,3.984,4.608,all P ＜ 0.05),and the differences were statistically significant in the improvement of the observation group compared with the control group(t =5.553,4.436,all P ＜ 0.05).The total effective rate of the observation group was 88.9％,that of the control group was 68.9％,the difference was statistically significant between the two groups (x2 =2.702,P ＜0.05).The incidence of adverse reactions in the observation group was 15.6％,which in the control group was 17.8％,the difference was not statistically significant between the two groups (x2 =0.040,P ＞ 0.05).Conclusion On the basis of conventional therapy,Danhong injection combined with edaravone in the treatment of acute ischemic stroke can significantly improve the clinical symptoms of the patients,improve the clinical efficacy and has good safety and great clinical significance.

Objective To discuss the medication rules of Glycyrrhizae Radix et Rhizoma in Shang Han Lun based on dose-response relationship;To provide references for clinical application. Methods It systematically arranged the provisions containing Glycyrrhizae Radix et Rhizoma in Shang Han Lun. It statistically analyzed the relationship among Glycyrrhizae Radix et Rhizoma and other various factors by using SPSS 20.0. Results After screening, 70 prescriptions were included, among which 2 (2.86%) contained raw Glycyrrhizae Radix et Rhizoma and 68 (97.14%) contained Glycyrrhizae Radix et Rhizoma Praeparata cum Melia. The taken dose of every time had a close relationship with the number of medicine, water consumption, residual water, water consumption per time and frequency of use, which had a statistical significance (P<0.01). Univariate logistic regression analysis showed whether Glycyrrhizae Radix et Rhizoma as a monarch medicine had the close relationship with the number of medicine (P=0.029). In 48(73.85%) prescriptions contained Glycyrrhizae Radix et Rhizoma Praeparata cum Melia as non-monarch medicine, the taken dose of every time and the number of drugs had statistical differences with that in the prescriptions which as monarch medicine (P=0.000). When Glycyrrhizae Radix et Rhizoma Praeparata cum Melia was in compatibility with other medicine, its dose had a certain rules with the number of medicine. When it was the basic medicine, there was no compatibility with Zingiberis Rhizoma Recens, Jujubae Fructus, and Ginseng Radix et Rhizoma. Conclusion It can be found from the dose-response relationship that Glycyrrhizae Radix et Rhizoma has a widely dose and combination efficacy in different prescriptions. Its flexibility, diversity and scientific nature provide the solid basis for clinical application.

Objective:To analyze the expression pattern and clinical significance of PBK /TOPK in cervical cancer.Methods:50 cases of low-grade cervical intraepithelial neoplasia ( CINI ) , 45 cases of cervical cancer and 40 cases of high-grade cervical intraepithelial neoplasia collected by pathology department during January 2012 to December 2014 in our hospital were selected ,and the expression of PBK/TOPK was detected by immunohistochemistry .The correlation between PBK/TOPK level and pathological characteristics were compared by statistical methods .Results: PBK/TOPK was expressed in 75.6% cervical cancer samples , much higher than that of 16.7%in non cervical cancer samples;the score of IS on CINI organizations was lower than that in the high-grade cervical intraepithelial neoplasia and cervical cancer ( P<0.05 ,P<0.01 ) ,and the score of IS in the high-grade cervical intraepithelial neoplasia was significantly lower than cervical cancer ( P<0.01 );the expression of PBK/TOPK and P53 in cervical cancer tissues were positively correlated by the non-parametric Spearman rank ( r=0.68 ,P=0.008 );the expression level of PBK/TOPK in cervical cancer significantly correlated with TNM stage,invasion and lymph node metastasis(P<0.05),but not with age and differentiation (P>0.05). Conclusion:PBK/TOPK expression was correlated with cervical cancer ,and it may be served as a molecular marker for clinical tumor diagnose and theapy .

Objective To explore whether microsatellite instability (MSI) is exsist in human gastric carcinoma and precancerous lesion.Methods The MSI was detected in gastric carcinoma and precancerous lesion by tissue laser capture microdissection and denaturing high performance liquid chromatogragh (DHPLC).Results MSI was found in 10.5 ％ intestinal metaplasia,21.12 ％ gastric hyperplasia and 43.75 ％gastric carcinoma respectively.In contrast,there was no microsatellite instability in normol gastric mucosa.It displayed accumulation and alteration from normal to cancerous tissues.Conclusion Pure tumor samples are obtained from tissue laser capture microdissection which could avoid the heteroplasmy of tissue.DHPLC is a high sensitive,fast and semiautomated method for identifying MSI.This experiment showed an increasing tendency of MSI incidence from precancerous lesion to gastric carcinoma.MSI could be used as a marker to predicate the occurrence of gastric carcinoma.

Improved medical postgraduate reexamination system is essential to the quality of students. In this study, organization of medical postgraduate reexamination was discussed from the perspectives of preliminary arrangements of medical postgraduate reexamination, fairness of reexami-nation process and improvement of the follow-up after the reexamination. Sufficient attention on the re-examination, effective supervision of the reexamination process and control of human factors were the three main factors for the successful medical postgraduate reexamination and effective guarantee for the improvement of postgraduate quality.

Objective To observe the clinical effect of long-range treatment for elderly patients with reflux esophagitis.Methods 124 reflux esophagitis patients were randomly divided into observation group (62 cases) and control group (62 cases) according to random number method.The observation group received long-range treatment (maintain treatment for 3 months after the end of the routine treatment),while the control group received conventional treatment,symptoms score and endoscopic healing of two groups were compared.Results Before treatment and 3 months after treatment,two groups of reflux symptom scores were not significantly different (t =1.325,1.241,all P ＞ 0.05),6 and 12 months after treatment,reflux symptoms score of the observation group was significantly lower than that of the control group (t =24.325,18.124,P ＜ 0.01),after total treatment,the good treatment effect rate of the observation group was 90.0%,which was higher than that of the contol group(55.7%)(x2 =17.902,P ＜0.01),healing of esophagitis excellent rate of the observation group was 86.7%,which was higher than that of the contol group (57.4%) (x2 =12.844,P ＜0.01).Conclusion Long-range treatment of symptomatic improvement has good effect in elderly the reflux esophagitis,the lesion healing to improve the quality,which is worthy of further promotion research.

Objective To investigate the clinical characteristics and the treatment of the infection of heli-eobacter pylori in gastric stump. Methods The clinical data of 210 patients who were examined by gastroscopy for gastric stump and 200 randomly selected patients who had no history of operation due to gastrointestinal diseases and received gastroscopy and Hp detections were retrospectively analyzed. Results The Hp infective rate in gastric stump was 26.2 %, and the Hp infective rates had no significant difference among different operative causes. The Hp positive rate after Billroth-Ⅰ operation was obviously higher than that after Billroth-Ⅱ type operation;the Hp posi-tive rate in the patients with bile regurgitation was lower than that in the patients without bile regurgitation. The Hp infective rate became lower as the time after subtotal gastrectomy prolonged. The clearance rate of anti-Hp treatment was 87.3 %. Conclusion The Hp infective rate in gastric stump is lower, but the Hp lesion in gastric stump ks ag-gravated. The anti-Hp therapeutic program which is commonly used at present plus gastric mucosa protectant and gastric motive power drugs are suitable for treating Hp infection in gastric stump, and have definite value in clinical application.

Mass spectrometry is an important and powerful tool for protein quantification. With the in-depth development of quantitative proteomics, limitations of classic MS based quantification methods, such as complicated matrix interference and throughput/capacity limitation, start to appear. Recent progress of series novel MS based techniques provide effective solutions for the limitations of relative and absolute proteomic quantification, including synchronous precursor selection ( SPS ) , mass defect isobaric labeling, parallel reaction monitoring ( PRM) , multiplexing acquisition ( MSX) , and various novel data independent acquisition ( DIA) modes. Here we summarized the current limitations of quantitative proteomics, reviewed the latest MS based quantification approaches, and discussed the features and advantages of these novel techniques for quantitative proteomic application.

Objective To observe the change of Visual Analogue Scale (VAS) and satisfaction with treatment in preventing remissive migraine by puncturing Taiyang (EX-HN 5) at different depths. Methods Forty eligible migraine patients were randomized into a treatment group and a control group, 20 in each group. The treatment group was intervened by deep needling at Taiyang (EX-HN 5) as the predominant treatment, while the control group was by superficial needling at Taiyang (EX-HN 5) as the predominant treatment. The VAS score and satisfaction degree with treatment were evaluated before and after treatment. Results The VAS score was significantly changed after intervention in the treatment group (P<0.01). After intervention, there was a significant difference in comparing the VAS score between the two groups (P<0.01). The satisfaction rate was 100.0% in the treatment group versus 85.0% in the control group, and the difference was statistically insignificant (P>0.05). Conclusions Compared to superficial needling at Taiyang (EX-HN 5), deep needling at Taiyang (EX-HN 5) can significantly reduce the headache degree..