1.The study of induction and differentiation of leukemic cell by phorbol ester
Xiaoli YUAN ; Quande LIN ; Xudong WEI
Journal of Leukemia & Lymphoma 2008;17(2):155-158
12-O-tetradecanoylphorbol-13-acetate (TPA) is an activator of protein kinase C (PKC)extracted from the croton oil, it is one of the most powerful inducers of differentiation as well as a powerful tumor promoter. It is used by the study of induction and differentiation generally, because of it can induce the differentiation of tumor cell and promote the lymphocyte transformation and secrete of lymphokine, meanwhile,it is able to induce many kinds of cell of leukemic cell strain like HL-60, HEL and so on, Some literature reported that TPA had the function of differentiation of primary leukemic cell. The article mainly explains the induction and differentiation of leukemic cell by phorbol ester as well as its mechanisms, which to provide the theory evidence for prevention and cure of leukemia.
2.Experience of treatment of subacute encephalopathy induced by 1, 2-dichloroethane poisoning.
Yuan-lin ZHOU ; Wei-jun HONG ; Shao-fa KE
Chinese Journal of Industrial Hygiene and Occupational Diseases 2009;27(4):253-254
Acute Disease
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Adult
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Ethylene Dichlorides
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poisoning
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Female
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Humans
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Male
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Neurotoxicity Syndromes
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therapy
3.Epidemiological analysis of occupational diseases in Zhejiang province China during 2006-2010.
Wei-ming YUAN ; Xing-lin FANG ; Bai-qi WU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(4):279-281
Adolescent
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Adult
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Aged
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China
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epidemiology
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Female
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Humans
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Male
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Middle Aged
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Occupational Diseases
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epidemiology
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Young Adult
4.Significance of monitoring indexes of coagulation and fibrinolytic before and after delivery
Liping YUAN ; Wei LIN ; Qingwen ZHU ; Gengqing CHEN
International Journal of Laboratory Medicine 2014;(16):2179-2180
Objective To investigate the significance of detection of blood coagulation and fibrinolytic indexes before and after delivery.Methods 212 cases in the postpartum hemorrhage group(bleeding amount ≥500 mL)and 235 cases in the normal deliver-y group were retrospectively analyzed.The prothrombin time(PT),international normalized ratio(PT-INR),activated partial throm-boplastin time(APTT),thrombin time(TT),fibrinogen(FIB),D-dimer(DD),blood platelets(PLT)and hemoglobin(HB)were de-tected before delivery and at 72 h after delivery.85 women with the physical examination were selected as the healthy group.Results Before delivery,PT/PT-INR,APTT,TT,PLT and HB in the normal delivery group were lower than those in the healthy group, while FIB and DD were higher than those in the healthy group;PT/PT-INR,APTT,TT and DD in the postpartum hemorrhage group were higher than those in the normal delivery group,while FIB and PLT were lower than those in the normal delivery group. At 72 h after delivery,there was no statistically significant differences in PT/PT-INR,APTT,TT and DD between the normal de-livery group and the healthy group(P >0.05),but FIB was higher than that in the healthy group;PT/PT-INR,APTT,TT and DD in the postpartum hemorrhage group were higher than those in the normal delivery group,while FIB,PLT and HB were lower than those in the normal delivery group.Conclusion Dynamically monitoring the change of the blood coagulation and fibrinolysis indexes before and after delivery has important significance in preventing postpartum hemorrhage and thrombosis.
5.Comparison of physiological indicators during living-donor liver transplantation in infants and children
Wei LIU ; Lin BO ; Mao YE ; Yuan SHI
Chinese Journal of Organ Transplantation 2014;35(11):676-680
Objective To explore the change characteristics of physiological indexes between infants and children during living-donor liver transplantation and discuss methods of regulation and control.Method In this study,42 patients were selected and assigned into two groups according to age:infants group (<1 year,n =25),and children group (1-16 years,n =17).The preoperative and peri-operative characteristics,intra-operative operation conditions,internal environment changes before and after re-perfusion,postoperative mechanical ventilation time,ICU time,hospital time,infection rate,additional surgery,complications and survival were analyzed.Result PELD (MELD) score,historical surgery rate and hematokrit were lower in children group than in infants group (P< 0.05).Serum creatinine and lactate concentrations increased significantly in children group as compared with infants group (P<0.05).Intra-operative an-hepatic phase and cold ischemia time were shortened significantly (P < 0.05),and incidence rate of re-perfusion syndrome was reduced in children group as compared with infants group (P<0.05).As compared with pre-re-perfusion,blood lactate concentrations were significantly raised only in infants group and glucose concentrations significantly raised only in children group (P<0.05).The blood levels of K + were decreased after reperfusion in both two groups,and those in infants group were lower than in children group (P< 0.05).Postoperative intensive care unit time was longer in children group than in infants group (P< 0.05),and there was no significant difference in survival rate between two groups.Conclusion There are many differences and change characteristics to physiological indexes between infants and children during the operation of living-donor liver transplantation.Timely management and regulation are critical for the success of surgery according to the differences.
6.Effect of Pirenzepine Injected Intravitreously on Retinal Blood Flow in Cats
yi-kang, DAI ; wei, WU ; lin, ZHANG ; ren-yuan, ZHU
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(03):-
0.05). In the group of pirenzepine, the RBF, velocity and volume significantly increased at 0.5 h and 1 h after injection compared with that before injection (P0.05). Conclusion These results suggest that pirenzepine could increase RBF and oxygen in blood with the peak time at 0.5 h and 1 h after intravitreous injection.
7.Expression and significance of SEL1L and p63 protein in esophageal squamous cell carcinoma and precarcinomacous lesion
lin, YUAN ; jia-wei, CHEN ; hong-hui, HU
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(11):-
Objective To investigate the expression of SEL1L and p63 protein in esophageal squamous cell carcinoma and precarcinomacous lesion.Methods Immunohistochemical staining(EnVision method)was employed to detect the expression of SEL1L and p63 protein in 60 samples of esophageal squamous cell carcinoma,32 samples of high grade esophageal intraepithelial neoplasia,13 samples of low grade esophageal intraepithelial neoplasia and 33 samples of normal esophageal mucosa.Results The positive rate of SEL1L protein expression was 61.5%in low grade intraepithelia neoplasia,90.6%in high grade intraepithelial neoplasia and 96.7%in esophageal squamous cell carcinoma,significantly higher than that in normal esophageal mucosa(6.1%)(P0.05).Conclusion Both the expression of SEL1L and p63 protein increases steadily in the progression of esophageal squamous cell carcinoma,which indicates that the two genes may play a role and cooperate with each other in the carcinogenesis.
8.Application of Cytological Examination of Cerebrospinal Fluid in Diag nosis of Central Nervous System Leukemia
yuan, GAO ; jia-ying, LIU ; lin-chun, JIANG ; wei, JIANG
Journal of Applied Clinical Pediatrics 2004;0(09):-
Objective To investigate the diagnosis value of the cytological examination of the cerebrospinal fluid of central nervous system leukemia.Methods Adopting cell smear centrifugal machine to collect the cerebrospinal fluid cells,the cells were stained and examinated under the microscope.Results Fifty-nine children with different type of leukemia had been examinated by 438 times by cerebrospinal fluid.The positive rates of the cases and samples were 15.3% and 8.7%,respectively.Conclusion The cytological examination of cerebrospinal fluid is especially valuable for the early diagnosis ,therapy and relapse of central nervous system leukemia of monitoring.
9.Expression of TAK1 and p38 genes in patients with acute myeloid leukemia and their clinical significance
Fangfang YUAN ; Ruihua MI ; Lin CHEN ; Xudong WEI
Journal of Leukemia & Lymphoma 2015;24(11):658-661
Objective To investigate the expression levels of TAK1 and p38 genes among different subtypes of acute myeloid leukemia (AML) patients,and to analyze the clinical characteristics of patients with different expression levels of TAK1 and p38 genes.Methods GAPDH was made as an internal reference,14 healthy people as control group.The quantitative real-time PCR was used to detect the expression of TAK1 and p38 in bone marrow samples of 87 AML patients,and the results were analyzed statistically.Results The expression levels of TAK1 and p38 in experiment group were higher than those in control group (0.194± 0.125 vs 0.015±0.008,0.233±0.140 vs 0.010±0.005,P < 0.001).TAK1 expression in M4 was higher than that in M2,M3 and M5 (P =0.005,0.000,0.002),TAK1 expression in M3 was lower than that in M2 (P =0.022).p38 expression in M4 was higher than that in M1,M2,M3 and M5 (P =0.013,0.035,0.000,0.045),as it was higher in M2 and M5 than that in M3 (P =0.001,0.012).The CD56 positive rate cells and the number of peripheral blood leukocytes of the TAK1 high expression group were higher than those of the TAK1 low expression group,the CD19 positive rate of the p38 low expression group was higher than that of the p38 high expression group.Conclusion The expression levels of TAK1 and p38 genes are elevated in AML patients,and the up-regulation may play an important role in the pathogenesis of AML.
10.Variance of Pathogenic Organisms in Purulent Endophthalmitis
Changlin ZHAO ; Lin WEI ; Yuan JING ; Chongde LONG
Chinese Journal of Nosocomiology 1994;0(01):-
OBJECTIVE To investigate the spectrum of organisms causing purulent endophthalmitis and to identify the variance of bacterial spectra and their sensitivities to commonly used antibiotics.METHODS The medical records of patients with purulent endophthalmitis receiving pathogen examination in Zhongshan Ophthalmic Center from Jan 2000 to Dec 2006 were analyzed retrospectively.RESULTS Of the 416 cases,82.0% purulent endophthalmistis were caused by ocular trauma and the organisms were isolated in 181(43.5%)of them.Among the pathogens isolated,14.9%,47.5%,9.9% and 27.6% were fungi,Gram-positive cocci,Gram-positive bacilli and Gram-negative bacilli,respectively.The fungal endophthalmistis was sporadic per annum.The most common bacterial pathogen was staphylococcus epidermidis(33.1%) which increased year by year.The most common Gram-negative bacterium was Escherichia coli(10.5%).Among the common pathogens,the Gram-positive cocci were sensitive to fluoroquinolones and cefoperazone,and the Gram-positive bacilli were sensitive to aminoglycosides and cefoperazone while the Gram-negative bacilli were resistant to various kinds of antibiotics in different degree.CONCLUSIONS The purulent endophthalmistis should be monitored in patients with open ocular injury.Fluoroquinolones with cefoperazone to treat the ocular inflammation are a preferred therapeutic regimen for patients with bacterial endophthalmitis with unknown etiopathogenesis.