Objective To analyze the relation between the CT changes of ventricular volume and prognosis in newborn of perinatal hypoxic-ischemic brain damage.Methods From Jan. 2002 to Dec. 2006, 105 newborns with perinatal hypoxic-ischemic brain damage (moderate to severe) were divided into 2 groups (group A with nervous system sequel and group B without nervous system sequal). Brain CT was performed in 24 hours after admission in all cases and at 1 and 3-month time point in some cases according to their convalescence condition. Huckman index,the third ventricular width on CT images of various time points were measured and calculated by radiologist. The relations between the values and prognosis were analyzed statistically.Results The huckman index increased gradually with rehabil (P

Defecation ; Humans ; Male ; Middle Aged ; Postoperative Complications ; physiopathology ; therapy ; Spinal Neoplasms ; surgery ; Urination

Objective To obtain the gene of murine Single chain Fv fragment (ScFv) against human cervical cancer and to express it in E. coli. Methods The variable region gene fragments of the heavy and light chains, which were amplified respectively using recombinant DNA techniques from CsA125 hybridoma cells, were spliced together through a flexible linker to ScFv against human cervical cancer. The ScFv genes were then cloned into expression vector pCANTAB 5E and expressed in E. coli HB2151 and TG1 respectively. The soluble ScFv were characterized by SDS PAGE and Western blot. The antigen-binding activities of the soluble and phage displayed ScFv were assayed by ELISA and cell immunohistochemical analysis. Results The expressed ScFv antibodies were soluble and phage displayed. The soluble ScFv secreted and expressed in E. coli HB2151 induced by IPTG were confirmed with SDS-PAGE, Western blot and ELISA. The specific binding capacity of the soluble and phage displayed ScFv to the surface associated antigen of human cervical cancer cell line was further confirmed with immunohistochemical studies. Conclusion The soluble and phage displayed ScFv expressed in E. coli against human cervical cancer showed high, specific affinity for the cervical cancer cell line surface associated antigen.

Objective To evaluate the influence of the preparation-to-colonoscopy interval, diet control and excise on bowel preparation quality in the split-dose method for colonoscopy. Methods Observational study, prospective cohort study design, three bowel preparation methods were set up in different departments for A, B, C 3 groups, each group of 110 cases. In group A, no movement was required. Diet preparation was 3 days earlier than the examination, and take all the medicine in once at 20:00 the day before examination, group B, 1 L medicine required for 20 minutes movement, diet preparation was 1 day earlier than the examination, and take the medicine in twice the day before examination at 14:00, group C, keep walking during taking the medicine, diet preparation was 1 day earlier than the examination, and took all the medicine in once the day before examination at 20:00. The Boston Bowel Preparation Scale was used to evaluate the bowel cleansing quality. A questionnaire was administered to investigate patients′tolerability and safety. Results A total of 330 consecutive patients undergoing colonoscopy. There was no significance among three groups on bowel cleansing quality(P>0.05). However, the number ofexcellently cleanedwas higher in group B than the others, in group A,B and C respectively 30, 46, 29 cases, the difference was significant (χ2=7.627, P=0.022). Group B was superior to the others in terms of comfort, sleep quality, hunger, and discomfort, the difference was significant (χ2=19.425, 6.687 and 8.130, P < 0.01 or 0.05). Group B was also superior to the others in terms of the compliance of completing the preparation under the doctors′advice (χ2=14.756, P=0.001) and exercises (χ2=11.848, P=0.001). Conclusions The new method shortened the time of the diet control and set the exercises time to 20 minutes. So it was found to have a higher level of safety, bowel preparation quality and tolerability.

OBJECTIVE:To establish a method for the main active components in Citrus chacheiensis with different storage time (1-19 years). METHODS:HPLC was conducted to determine the content of hesperidin:the column was Diamonsil C18 with mobile phase of methanol-acetic acid-water(35∶4∶61,V/V/V)at a flow rate of 1.0 ml/min,detection wavelength was 283 nm,col-umn temperature was 25 ℃,and the injection volume was 5 μl;the contents of nobiletin and tangeretin:the column was Diamon-sil C18 with mobile phase of water-methanol(gradient elution)at a flow rate of 1.0 ml/min,detection wavelength was 326 nm,col-umn temperature was 25 ℃,and the injection volume was 10 μl;and the content of synephrine:the column was Diamonsil C18 with mobile phase of methanol-potassium dihydrogen phosphate solution(taking 0.6 g potassium dihydrogen phosphate,1.0 g sodi-um dodecyl sulfate,1 ml glacial acetic acid dissolved to 1 000 ml)(65∶35,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 275 nm,column temperature was 25 ℃,and the injection volume 20 μl. RESULTS:The linear range was 500-4 500 ng for hesperidin(r=0.999 8),38.816-388.16 ng for nobiletin(r=0.999 6),19.936-199.36 ng for tangeretin(r=0.999 5)and 640-2 560 ng for synephrine(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 3%;recoveries were 96.42%-102.75%(RSD=2.54%,n=6),97.42%-99.95%(RSD=2.46%,n=6),99.26%-106.19%(RSD=2.31%,n=6) and 97.47%-99.76%(RSD=1.95%,n=6),respectively. CONCLUSIONS:The method is simple and stable with good reproducibility, and can be used for the contents determination of main active components in C. chacheiensis with different storage time. Pericarpi-um citri“the older the better”may be irrelevant to the change of the contents of the above-mentioned 4 active components,and it is speculated related to the release of volatile oil content to ease dryness.

38 patients with dentition defect in our hospital for magnetic attachment dental treatment were analyzed. At the same time,34 cases of patients with dentition defects were ,being treated with conventional prosthodontics, selected to compare the stability of overdentures and masticatory function with magnetic attachment. The stability of overdentures and masticatory function after restored with magnetic attachment was significantly higher than before(P< 0. 01),while there was no difference after 4 weeks. Cementation language,comfort and appearance satisfaction after 4 weeks were significantly higher than before(P < 0. 05). The stability of overdentures,masticatory function, cementation language,comfort and appearance satisfaction after restored with magnetic attachment was significantly higher than conventional prosthodontics(P < 0. 05). It finally indicated that magnetic attachment had the advanta-ges of comfort,convenience and good effect. The stability of overdentures,masticatory function and application sat-isfaction was superior to conventional prosthodontics,and it had an application prospect.

Objective To investigate allele frequency distributions and haplotype polymorphism of ten Y-chromosome short tandem repeats (STR) loci of Han population in Zhuhai area and evaluate their applicability to forensic cases. Methods DYS393, DYS19, DYS389 Ⅱ, DYS390, DYS391, DYS389 Ⅰ, DYS439、DYS438、DYS392 and DYS385 loci were amplified with Y-plex~(TM) PCR amplification Kit and analyzed by ABI 310 genetic analyzer. 200 unrelated male individuals of Han nationality in Zhuhai were recruited for determination of allele frequencies and haplotype data. Results 5, 6, 6, 5, 4, 5, 5, 5, and 7 alleles were found in 9 of the 10 Y-STR loci, respectively and 44 haplotypes were detected at DYS385 locus. The range of gene diversity (GD) was determined between 0.3904 for DYS391 and 0.9497 for DYS385. Total 161 haplotypes were found at the 10 Y-STR loci, of which 134 occurred once, 20 occurred twice, 3 occurred three times, 3 occurred four times, and 1 occurred five times. The cumulative GD was 0.9948. Conclusion The 10 Y-STR loci are highly polymorphic and suitable for forensic individual identification and paternity testing in Zhuhai Han population.

Objective To explore the antiepileptic effect and mechanism of glucocorticoid. Methods Animal behaviour observation and immunocytochemical staining. Results Major epilepsy was induced by pentylenetetrazole(PTZ).Dexamethasone or diphenylhydantoin(DPH) administered in rat 30?min before administration of PTZ could suppress or inhibit epileptiform.Immunocytochemistry demonstrated that there was a large number of hypertrophic astrocytes with GFAP immunoreaction in cerebral cortex,hippocampal gyrus and dentate gyrus of epileptic rats induced by PTZ.The immunoreaction of GFAP was obviously weakened,the number of positive cells was reduced,the processes were shorter and less in both groups of antiepilepsy by GC or DPH.The expression of Fos protein was in a great quantity in cerebral cortex 1 to 1\^5 hours after seizure induced by PTZ,whereas their expressions were remarkable suppressed in GC or DPH antiepileptic groups. Conclusion 1\^The antiepileptic effect of GC was further proved by comparing with the antiepileptic effects of DPH(a traditional antiepileptic drug). 2\^Inhibited activity of astrocytes might be involved in antiepileptic mechanism of GC. 3\^The change of expression of Fos protein might be closely related to epileptic actions. [

Objective To investigate the distribution of interleukin\|2 receptor (IL\|2R) and glucocorticoid receptor(GR) and identify coexistence of IL\|2R and GR in the rat brain. Methods The double labeling immunocytochemical technique(PAP method combined with ABC method), DAB and BDHC were used in the double labeling immunocytochemical method as the chromogens respectively. The reactive products of former was brown or yellow and later was black blue. Results IL\|2R and GR positive neurons were widely distributed in the cerebral cortex, hippocampus, thalamus, many motor and sense nuclei in the brain stem. The immunoreactive products of IL\|2R were found to be located on cell membrane and GR in nucleus and cytoplasm. There were a lot of positive double labeling neurons in the rat brain. The rate of double labeled cells in the total number of positive cells varied in different regions of brain, such as, 50 percent in cerebral cortex and 30 percent in nucleus of abducent nerve. Conclusion Immunogical cytokines and hormone could regulate the neuronal function through their corresponding receptors which coexisted in the same brain neurons. The present study might provide morphological evidence in the level of receptor for the immuno\|neuro\|endocrine network.

Objective To analyze the clinical effects of continuous epidural anesthesia and psychological intervention in painless labor. Methods From June 2016 to December 2016110 cases of maternal treatment in Ningbo city town of dragon longsai hospital as the research object, randomly divided into control group and observation group, control group of women to provide continuous epidural anesthesia, the observation group in continuous epidural anesthesia combined with psychological intervention measures. Results The two groups of pregnant women at different time scales VAS score, duration and other indicators, the data into the SPSS software, given the corresponding analysis and draw conclusions. Results The two groups of pain VAS score had no significant difference, the observation group each time after analgesia VAS score decreased than the control group(P<0.05); the first stage of the observation group was shorter than the control group (P<0.05), there was no significant difference between the 2 groups of second, third labor time. Conclusion Conclusion Epidural anesthesia and psychological intervention combined with painless labor have good analgesic effect and shorten the first stage of labor. .