1.Effects of berberine on Insig-2 and VDR gene expression of HepG2 cell line
Wei CHANG ; Qixin ZHOU ; Junxia YANG
Chinese Traditional Patent Medicine 1992;0(07):-
AIM: To observe the mRNA expressions of Insulin-induced gene 2(Insig-2) and Vitamin D receptor(VDR) in HepG2 cell line and investigate the possible mechanism of berberine on regulating blood lipid. METHODS: HepG2 cells were incubated with Bet at different concentrations(1,3,10,30,100 ?mol/L),and incubated with 3 ?mol/L berberine for various times(0,12,24,48,72 h).Total RNA was extracted and the mRNA expressions of Insig-2 and VDR from HepG2 cells treated by berberine were quantified by RT-PCR.(RESULTS): After exposure to gradient concentrations of berberine for 24 h,the mRNA expressions of Insig-2 and VDR in HepG2 cells increased obviously peaked at 3 ?mol/L concentration,then the up-regulating effect declined gradually.The relative coefficient between them was(0.753)(P
2.Chronic wound:pathogenesis and current treatments
Fei CHANG ; Changwei YANG ; Wei LU
Academic Journal of Second Military Medical University 2000;0(11):-
Chronic wound has very complicated pathogenesis and is difficult to treat;the situation has been improving owing to the progress in wound healing research and the clinical application of new techniques.This article focuses on the pathogenesis of chronic wound and introduces progress in various kinds of treatment,such as skin substitute,growth factors,negative pressure treatment,etc.
3.Study on modulation for immunosupperssion by WWOX in Lewis lung cancer cells
Wei YANG ; Hongyan YUAN ; Yaping CHANG
Chinese Journal of Immunology 1985;0(02):-
Objective:To investigate the mechanism of WWOX regulating immunosuppression in Lewis lung cancer cells.Methods:Transfected pCDNA4.0/myc-WWOX plasmid was transfected into Lewis lung cancer cells to detected the effect of cell supernatant on generation of lymphocytes by MTT method,and mRNA expression of immunosuppression factors by semi-quantitative RT-PCR.Function change of NK,CTL to kill tumor and lymphocte proliferation were analyzed by MTT and tumor volume.Results:The suppression of the supernatant to generation of lymphocytes was present compared with control group(P
4.Construction of recombinant adenovirus vector expressing anti-VEGF hairpin ribozyme and human interleukin-24 and its cytotoxic effect on colon cancer HT-29 cells.
Shu-Jian CHANG ; Ji-Cheng YANG ; Wei-Chang CHEN
Chinese Journal of Oncology 2009;31(6):433-433
Adenoviridae
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genetics
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Cell Death
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Genetic Vectors
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HT29 Cells
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Humans
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Interleukins
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biosynthesis
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genetics
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RNA, Catalytic
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biosynthesis
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genetics
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RNA, Messenger
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metabolism
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Recombinant Proteins
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biosynthesis
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genetics
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Transfection
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Vascular Endothelial Growth Factor A
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metabolism
5.Specific role of synovial macrophages in rheumatoid arthritis
YANG XUE-ZHI ; CHANG YAN ; WEI WEI
Chinese Journal of Pharmacology and Toxicology 2017;31(10):1026-1026
Rheumatoid arthritis (RA) is an autoimmune disease, which is characterized by synovial inflammation. Hyperplasia sublining macrophages found in synovium is an early hallmark of RA and effective treatment results in their diminution. However, the origin of these sublining macrophages in synovium (including infiltrated macrophages and tissue-resident macrophages) are still unknown both in animal models of arthritis and RA patients, let alone the differences and feature of these macro?phages. In rheumatic synovium, macrophages are submitted to a large variety of micro-environmental signals which influence the phenotypic polarization and activation of macrophages. Understanding the mechanisms and functional consequences of the heterogeneous macrophages will contribute to confirm their potential role in synovial inflammation development. Furthermore, research on macrophage plasticity to soft-control their phenotypic polarization could lead to novel therapeutic approaches.
6.Review and Outlook of the Developing Path of Bioethics
Wei YANG ; Ping DU ; Yunli CHANG ; Fang YANG
Chinese Medical Ethics 1995;0(02):-
As a young discipline imported from the West,Chinese bioethics has made tremendous achievements in the last three decades.However,at the same time,there are also certain confusions about its ideaistic origins and academic development.Observing the trends in the growth of western bioethics and the changes in the contemporary real social life,we can see that it is the time to break the current bottleneck for the contemporary development of Chinese bioethics.Specifically,Chinese bioethics should shift its attention to the quality of life,and manifests its thought depth and academic value through reflecting the reality.Advancing with the times,the bioethics is bound to have a brighter future.
7.Effects of Bone Marrow Mesenchymal Stem Cells Transplantation on Expression of Growth Associated Protein-43 in Rats after Spinal Cord Injury
Bingkui LI ; Bin ZENG ; Wei CHANG ; Dayi WANG ; Qi YANG
Chinese Journal of Rehabilitation Theory and Practice 2015;(12):1391-1396
Objective To observe the effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on the expression of growth associated protein-43 (GAP-43) in rats after spinal cord injury (SCI). Methods The SCI models were made by impacting at the level of T11 segment. After modeling, the rats were randomly and equally divided into control group (n=18) and observation group (n=18). 1 week after injury, DMEM/F12 medium 0.1 ml was injected into tail vein of rats in the control group, and an equal volume of BMSCs suspension was injected in the observation group. 1, 2, 4, 8 weeks after SCI, the hindlimb function was evaluated by the Basso-Beattie-Bresnahan (BBB) score. 2, 4, 8 weeks after SCI, the expression of GAP-43 mRNA was detected by RT-PCR, and the expression of GAP-43 was detect-ed by the immunohistochemistry. Results 2, 4, 8 weeks after SCI, compared with the control group, The BBB score increased, the expres-sion of GAP-43 mRNA and GAP-43 increased in the observation group (P<0.05). Conclusion BMSCs transplantation can improve the ex-pression of GAP-43 in gene and protein level, and then promote the repair of SCI.
8.Effects of HMGA1 silence by RNA interference on the apoptosis and cell cycle of hepatocarcinoma cells
Mingguang ZHU ; Xin ZHANG ; Hongyan YUAN ; Wei YANG ; Yaping CHANG
Chinese Journal of Immunology 2001;0(07):-
Objective:To develop HMGA1-silencing SMMC-7721 cell and investigate its impacts on apoptosis and cell cycle in tumor cells.Methods:Constructing HMGA1-silencing SMMC-7721 cell through G418 selection of RNAi plasmid transfected cells;surveying the apoptosis,prliferation and cell cycle of the tumor cells by FACS and MTT.Results:Stable HMGA1-silencing cells were obtained successfully.The apoptosis percentage in RNAi group(29.46?3.04%) was significantly higher than that in the negative control (1.96?0.76%) or control (2.04?0.70%)within each P
9.Study of professional nursing training in promoting nurses' humanistic quality
Fengying XI ; Guihua YANG ; Zongxia CHANG ; Wei YUAN
Chinese Journal of Practical Nursing 2010;26(30):38-39
Objective To integrate nurses' humanistic quality into professional nursing training, in order to improve humanistic quality and nursing quality. Methods Nurses with working years within 6 years in our hospital were chosen from May, 2009 to May, 2010. And through non-professional reading, specified materials reading, multimedia lectures, particularly the refining and essence of reading reports, humanities knowledge and humanistic care consciousness was melted into personal morality, words, deeds and cultivation. Investigation about satisfaction degree was carried out among patients before and after the training. Results Satisfaction degree of patients increased after training, so did the satisfaction degree of nurses to reading reports. Conclusions To integrate nurses' humanistic quality into professional nursing training can improve humanistic quality of nurses and nursing quality. Nurses' professional image will be increasingly standard, overall quality and service quality can be improved, and thought of dedicated service for patients will be consolidated.
10.The significance of introducing registry study in the post-marketing safety research for Chinese medicine and pharmacy.
Xing LIAO ; Yan-Ming XIE ; Wei YANG ; Yan-Peng CHANG
Chinese Journal of Integrated Traditional and Western Medicine 2014;34(3):261-266
There is a new research model named 'registry study/patient registry' in Western medicine, which could be referred to by Chinese medicine researchers, such as active safety surveillance. This article will introduce registry study from different aspects as the developing history, features, and application in order to inform Chinese medicine researchers of future studies.
Marketing
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Medicine, Chinese Traditional
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adverse effects
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economics
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Phytotherapy
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adverse effects
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economics
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Registries
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Safety Management
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methods