Objective To observe the changes of eosinophils (EOS) in peripheral blood in patients with acute myocardial infarction (AMI).To investingate the association of EOS in peripheral blood and acute myocardial infarction.Methods The single-centre study enrolled 50 acute myocardial infarction patients (23 cases were NSTEMI and 27 cases were STEMI) who were treated in Beijing Tiantan Hospital from January 2014 to July 2014.Fifty medical individuals who were randomly chosen from the same period of time.White blood cell counts,EOS counts and percentages, levels of Troponin Ⅰ of all individuals were determined.Compare White blood cell counts,EOS counts and percentages between two groups.Association of EOS in peripheral blood and Troponin Ⅰ in patients with acute myocardial infarction was evaluated by Pearson correlation.Results The levels of eosinophils counts ((0.02±0.01) × 109/L vs.(0.24±0.14) × 109/L) and percentages ((0.40±0.49) ％ vs.(1.25±0.54)％) in AMI patients were significantly lower than those in the control group (t =11.083, P＜0.01;t=8.242,P＜0.01).There was significant inverse correlation between EOS counts and percentage and levels of Troponin Ⅰ(r=-0.390,-0.387;P＜0.05).Conclusion Patients with acute myocardial infarction presented decreased eosinophils counts and percentages.EOS counts and percentage and levels of Troponin Ⅰ presented negative correlation.It was close relation of EOS and acute myocardial infarction.

Adult ; Female ; Humans ; Lead Poisoning ; complications ; Occupational Diseases ; chemically induced ; Optic Nerve Diseases ; chemically induced

Objective: To investigate the prevalence and clinical significance of Modic changes in endplates of cervical vertebral body in patients with cervical spondylotic myelopathy(CSM). Methods: The T1-weight and T2-weight sagittal MRI scans of 136 CSM patients undergoing anterior operation were retrospectively reviewed. The patients' age, gender, prevalence, precise vertebral levels and specific type of Modic changes were recorded, and the association of axial pain with Modic changes was analyzed. Results: Modic changes were observed in 23 patients (16.9%), including 17(17.7%) male and 6(15%) female. The most frequent cervical spinal levels of Modic changes was C5-6. Of all the patients, 4.4% had type I Modic change, 7.4% had type II, and 5.1% had type III. The incidence of axial pain was 56.5% in Modic change groups and 20.4% in non-Modic change groups; there was significant difference between the two groups (P<0.05). Postoperatively, the axial pain was relieved in 76.9% in Modic change groups and 73.9% in non-Modic change groups (P>0.05). The pre-operation incidences of axial pain in patients with type I, type II and type III Modic change were 83.3%, 60% and 28.6%, respectively; and the post-operation pain-relieving rates were 100%, 66.7% and 50%, respectively. Conclusion: The most common Modic change is type II in the cervical spine, with the C5-6 level being the most frequently involved. The incidence of axial pain is high in patients with Modic change, especially those with type I.

Objective To investigate the effect of Bax and Bcl-X_L expression in newborn rat with moderate hyperoxic exposure. Methods Hyperoxic lung injury model was established by exposure to 60% O_2 in the neonatal period of SD rats. Rats exposed to air were used as control groups, with 8 animals in each group on repeated experiments. The pathology of pulmonary tissues was detected by HE stain. Mean alveolar area and alveolar number per ?m~2 were applied to estimate the pathological effects of prolonged hyperoxia in neonatal rats. The expression of Bax and Bcl-X_L proteins in lung were detected by immunohistochemistry and the expressions of Bax and Bcl-X_L mRNA by RT-PCR. Results In hyperoxia groups, alveolar dysplasia appeared 4 days after hyperoxia, mean alveolar area increased and alveolar number per ?m~2 decreased from the 4th day. Bax and Bcl-X_L protein were mainly expressed on bronchiolar epithelial cells and vascular endothelial cells. Compared with control group, the expression of Bax increased from the 1st day after hyperoxia, Bax mRNA decreased from the 11th day (q=8.4802, P

Gastrointestinal Hemorrhage ; drug therapy ; etiology ; prevention & control ; Humans ; Hypertension, Portal ; complications

Objective To examine the effect of endotoxin on myocardial lipid metabolism and determine it′s possible mechanism Methods Sixty nine male Wistar rats (350 450g) were randomly divided into 2 groups: in vivo group and in vitro group In in vivo group endotoxin 0 1?g?kg -1 (small dose)or 1mg?kg -1 (large dose) was injected intrabdominally 3h after endotoxin administration the animals were anesthetized Heart was excised and passively perfused in a Laugendorff apparatus with oxygenated Krebs Heuseleit buffer at 37℃ Non esterified fatty acids (NEFA)and triacylglycerols(TAG) were used as energy supplying substance when the isolated heart had been stabilized for 15min NEFA or TAG was slowly added to the perfusate circuit 1 ml of perfusate was drawn every 10 min until the end of experiment (80 min ) for determination of the effects of endotoxin on NEFA or TAG oxidation and utilization by isolated heart At the end of experiment heart was harvested and frozen for measurement of myocardial lipids and lipoprotein lipase activity In in vitro group endotoxin was added to perfusate (1mg/ml) after the heart was excised and passively perfused In control group endotoxin was replaced by normal saline Results Large dose of endotoxin administered in vivo decreased NEFA oxidation rate by 30% in perfused isolated heart and caused increased accumulation of lipid in myocardial tissue By contrast endotoxin administered in vitro increased oxidation rate of TAG accompanied by an increase in myocardial lipoprotein lipase activity Administration of endotoxin in vivo and in vitro also caused accumulation of various exogenous lipids in myocardium in both NEFA and TAG perfused heart Conclusions This study shows that endotoxin has a significant effect on myocardial lipid metabolism The effect of endotoxin could be either direct or indirect according to the different lipids available

Objective To explore the reparative effect of bactericidal/permeability-increasing protein(BPI) on the damaged mucosa of rats with otitis media with effusion (OME),and state the pathogenesis of OME.Methods Wistar rats(40 ears) were randomly divided into six groups:normal control group (n=4),BPI control group(n=4),eustachian tube obstruction (ETO) group (n=8),lipopolysaccharide (LPS) injection group (n=8),ETO+LPS group (n=8),ETO+LPS+BPI group (n=8).The experimental OME model was made through eustachian tube obstruction and LPS injection.The rats were killed after 1,2 and 4 weeks and the changes of mucosa of middle ear were observed under light and scanning electron microscope.Results The rats in normal control group and BPI control group had the normal mucosa in the tympanic orifice of the eustachian tube.It consisted of pseudostratified ciliated cubical or columnar epithelium which contained an abundant number of ciliated cells and a few goblet cells,these were the mucociliary clearance system of the middle ear.The hypotympanum consisted of thin,squamous epithelium with few microvillus.Middle ear mucosa was obviouly thickened in LPS injection,ETO and ETO+LPS groups.An increase in goblet cells and a decrease in ciliated cells were observed in the tympanic orifice of the eustachian tube.The epithelial layer in the hypotympanum had become more pseudostratified ciliated cubical epithelium.In ETO+LPS+BPI group,there was thin squamous epithelium in the hypotympanum near normal,which was not thickened and contained few microvillus. Conclusion LPS and ETO can result in the occurrence and protracted courses of OME by mimosa's inflammatory reaction which can reduce the activity of ciliary cells and weaken the function of mucociliary clearance system.BPI could bind avidly to LPS,reduce inflammatory reaction,and break the inflammatory cycle and reestablish an effective mucocillary clearance system.The results suggest that BPI treatment is a potential effective drug for prevention and therapy of chronic OME.

Objective To investigate the efficacy and safety of intravitreous injection with triamcinolone acetonide (TA) for cystoid macular edema (CME) due to central retinal vein occlusion (CRVO). Methods Fourteen eyes of 14 patients with CME due to CRVO underwent intravitreous injection with 0.1 ml TA (40 mg/ml). Best-corrected visual acuity, intraocular pressure (IOP), slitlamp examination, fundus fluorescein angiography, and optical coherence tomography (OCT) were performed on the patients before and after the injection. The follow-up period was 10-22.4 months, with the mean of 15.9 months. Results The average visual acuity was 0.1 before the treatment; while 1 month and 3 months after the injection, the visual acuity of all of the patients improved, including ≥0.2 in 71.43% and 63.6% of the patients, respectively, and ≥0.5 in 42.9% and 27.3%, respectively. After then, the visual acuity of some patients decreased, and in the final visit, 4 eyes (28.6%) had a visual acuity of ≥0.2, and 1 eye (7.1%) of ≥0.5. Compared with that before the treatment, the visual acuity of 10 (71.4%) eyes improved and 4 (28.6%) eyes declined. One month after the treatment, the macular edema disappeared in 10 eyes (71.4%) and alleviated in 4 (28.6%). In the final visit, macular edema disappeared in 4 eyes, alleviated in 9, and aggravated in 1. In the follow-up duration, high IOP [22.3-40.1 mm Hg (1 mm Hg=0.133 kPa)]. In the final visit, posterior subcapsular cataract was found in 7 eyes. Conclusion Intravitreous injection with TA may be effective in reducing CME and enhancing the visual acuity in a short term with high IOP in some eyes. In the long-term follow-up period, the rate of recurrence of CME and incidence of posterior subcapsular cataract is high.

Objective To study the establishment methods of virtual three-dimensional cerebral aneurysm models by using three-dimensional moulding software.Methods The virtual model including the cerebral arteries and the aneurysms were established by using the three-dimensional moulding software of 3D Studio MAX R3 in 20 cases of normal cerebral DSA image and 216 cases of cerebral aneurysms DSA image as the original.Results ①In comparison with DSA image,the virtual cerebral arteries possessed similarity in appearance,and had a benefit for understanding the vascular three-dimensional spatial relation in visual sense.The virtual cerebral arteries could display the virtual guide wire and catheter in semitransparent.The several models of different variant anatomy could be established easily based on the copy files of the virtual cerebral arteries model;②In comparison with DSA image,virtual cerebral aneurysm could display the location of development,direction of growth and feature of appearance correctly.The several aneurysms models of different location,direction of growth and appearance could be established easily based on the copy files of the virtual cerebral aneurysms model.Conclusion The virtual three-dimensional cerebral aneurysm models that were established by the software method can display the three-dimensional spatial relation of the cerebral arterial system and aneurysmal shape distinctly,and would be useful in assisting morphology study and guiding clinical work.

Objective To investigate the effect of propofol and fentanyl on inflammatory response to tissue injury in children undergoing open heart surgery with CPB and the possible mechanism. Methods Twenty HYHA class Ⅱ - Ⅲ children with congenital heart disease, aged 10 month-12yr, undergoing elective open heart surgery were randomly divided into two groups: group F in which anesthesia was maintained with high dose fentanyl (50-80?g? kg-1 ) and group FP in which anesthesia was maintained with propofol infusion (20mg?kg-1?h-1 ) and intermittent iv boluses of fentanyl (